BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0048.p (440 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGH9.1 B3KGH9_9BACI 6-phosphogluconate dehydrogenase, decarbox... 805 0.0 B1DHS1.1 B1DHS1_9BACL 6-phosphogluconate dehydrogenase, decarbox... 743 0.0 Q2B6E0.1 Q2B6E0_9BACI 6-phosphogluconate dehydrogenase, decarbox... 698 0.0 Q5KXF7.1 Q5KXF7_GEOKA 6-phosphogluconate dehydrogenase, decarbox... 696 0.0 P80859.4 6PGD2_BACSU 6-phosphogluconate dehydrogenase, decarboxy... 694 0.0 >B3KGH9.1 B3KGH9_9BACI 6-phosphogluconate dehydrogenase, decarboxylating EC=1.1.1.44 Length = 473 Score = 805 bits (2079), Expect = 0.0, Method: Compositional matrix adjust. Identities = 380/438 (86%), Positives = 408/438 (93%) Query: 1 VYNRSPEKTHDLINEAEGKNLTGTFSIEEFVESLEVPRKILIMVQAGKATDATIEQLLPH 60 VYNRSP+KT D++ E EGK + GTFSIEEFV+SLE PRKILIMVQAG+ATDATIEQL+PH Sbjct: 35 VYNRSPQKTEDMLKETEGKQVKGTFSIEEFVDSLETPRKILIMVQAGQATDATIEQLIPH 94 Query: 61 LDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFIGTGVSGGEEGALKGPSIMPGGQESAYK 120 LDQGDIIIDGGNAYFPDT RR+KEL KGF FIG GVSGGEEGALKGPSIMPGGQESAYK Sbjct: 95 LDQGDIIIDGGNAYFPDTQRRSKELAEKGFNFIGAGVSGGEEGALKGPSIMPGGQESAYK 154 Query: 121 LVEPILTAISAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLIGEAYHLLKDVLGLD 180 LVEPILTAISAKVN EPCCTYIGPDGAGHYVKMVHNGIEYGDMQLI EAY LLKDVLGLD Sbjct: 155 LVEPILTAISAKVNEEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLICEAYQLLKDVLGLD 214 Query: 181 AKELHSIFADWNSGELDSYLIEITKDIFAEYDEETGKPMVDVILDAAGQKGTGKWTSQSS 240 AK+LH IF +WN GELDSYLIEIT DIF++YDEETGKPMVDVILD AGQKGTGKWTSQSS Sbjct: 215 AKDLHGIFTEWNQGELDSYLIEITADIFSKYDEETGKPMVDVILDTAGQKGTGKWTSQSS 274 Query: 241 LDLGVPLSMITESVFSRFLSAMKEERVAASKVLSGPVTEPFQGDKAEFIENVRKALFASK 300 LDLGVPLSMITESVFSRFLSAMKEERVAASK+L+GP T F GDKAEFIENVRKALFASK Sbjct: 275 LDLGVPLSMITESVFSRFLSAMKEERVAASKILNGPKTAAFDGDKAEFIENVRKALFASK 334 Query: 301 IVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLLL 360 IVSYAQGFAQLRVAS+EY W+L+YGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLLL Sbjct: 335 IVSYAQGFAQLRVASDEYGWNLQYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLLL 394 Query: 361 DPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFSSALAYYDSYRTERLPANLLQAQRDYFG 420 DPFF +I+++YQ AWR+VV++AV+ G+PVPGFSSALAYYDSYRT LPANLLQAQRDYFG Sbjct: 395 DPFFTDIIESYQDAWRKVVASAVSLGIPVPGFSSALAYYDSYRTANLPANLLQAQRDYFG 454 Query: 421 AHTFKRVDKEGVFHHNWL 438 AHTFKRVDKEG FH+NW+ Sbjct: 455 AHTFKRVDKEGTFHYNWM 472 >B1DHS1.1 B1DHS1_9BACL 6-phosphogluconate dehydrogenase, decarboxylating EC=1.1.1.44 Length = 470 Score = 743 bits (1917), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/439 (79%), Positives = 396/439 (90%) Query: 1 VYNRSPEKTHDLINEAEGKNLTGTFSIEEFVESLEVPRKILIMVQAGKATDATIEQLLPH 60 VYNRS EKT +LI E+ GKNL +++EEFV+SLE PRKILIMVQAG TDATI+ L+PH Sbjct: 31 VYNRSREKTDELIKESAGKNLAPAYTVEEFVQSLESPRKILIMVQAGAGTDATIDALVPH 90 Query: 61 LDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFIGTGVSGGEEGALKGPSIMPGGQESAYK 120 LD+GDIIIDGGNAYFPDTVRR+K LE +G RFIGTGVSGGEEGALKGPSIMPGGQESAYK Sbjct: 91 LDKGDIIIDGGNAYFPDTVRRSKALEEQGIRFIGTGVSGGEEGALKGPSIMPGGQESAYK 150 Query: 121 LVEPILTAISAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLIGEAYHLLKDVLGLD 180 LVEPILTAISAKV + CCTYIGPDGAGHYVKMVHNGIEYGDMQLI EAY LLK+ L L+ Sbjct: 151 LVEPILTAISAKVGDDACCTYIGPDGAGHYVKMVHNGIEYGDMQLICEAYDLLKNTLDLN 210 Query: 181 AKELHSIFADWNSGELDSYLIEITKDIFAEYDEETGKPMVDVILDAAGQKGTGKWTSQSS 240 A+ELHSIF +WN GELDSYLIEIT DIF++YD ETGKPMVDVILD+AGQKGTGKWTSQSS Sbjct: 211 AEELHSIFTEWNKGELDSYLIEITADIFSKYDPETGKPMVDVILDSAGQKGTGKWTSQSS 270 Query: 241 LDLGVPLSMITESVFSRFLSAMKEERVAASKVLSGPVTEPFQGDKAEFIENVRKALFASK 300 LDLGVPLS+ITESVF+RFLSAMK+ER+AASKVL+GP +PF+GDK FIE+VRKALFASK Sbjct: 271 LDLGVPLSIITESVFTRFLSAMKDERIAASKVLNGPEKKPFEGDKVAFIESVRKALFASK 330 Query: 301 IVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLLL 360 I SYAQGFAQ+R ASEEY+W+L+YG++A I+RGGCIIR+RFLQNI DAY+ + L+NLLL Sbjct: 331 ICSYAQGFAQMRAASEEYDWNLRYGDIAMIFRGGCIIRARFLQNIKDAYDRDANLRNLLL 390 Query: 361 DPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFSSALAYYDSYRTERLPANLLQAQRDYFG 420 DP+F+ I+D+YQ AWR+VV AVA GVPVP F+SAL+Y+DSYRTERLPANLLQAQRDYFG Sbjct: 391 DPYFQGIVDSYQGAWREVVGTAVANGVPVPAFASALSYFDSYRTERLPANLLQAQRDYFG 450 Query: 421 AHTFKRVDKEGVFHHNWLS 439 AHTFKR+DKEG FHHNW++ Sbjct: 451 AHTFKRLDKEGSFHHNWMN 469 >Q2B6E0.1 Q2B6E0_9BACI 6-phosphogluconate dehydrogenase, decarboxylating EC=1.1.1.44 Length = 468 Score = 698 bits (1802), Expect = 0.0, Method: Compositional matrix adjust. Identities = 325/438 (74%), Positives = 380/438 (86%) Query: 1 VYNRSPEKTHDLINEAEGKNLTGTFSIEEFVESLEVPRKILIMVQAGKATDATIEQLLPH 60 VYNRS EKT +++ E+EGKN+TGT+SIEEFV SLE PRKI++MV+AG TDATIEQL PH Sbjct: 31 VYNRSSEKTEEMLKESEGKNITGTYSIEEFVNSLEKPRKIMLMVKAGGPTDATIEQLKPH 90 Query: 61 LDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFIGTGVSGGEEGALKGPSIMPGGQESAYK 120 L++GDI+IDGGN +F DT RRNKEL G FIGTGVSGGEEGALKGPSIMPGGQ+ AY Sbjct: 91 LEKGDILIDGGNTFFADTQRRNKELSELGIHFIGTGVSGGEEGALKGPSIMPGGQKEAYD 150 Query: 121 LVEPILTAISAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLIGEAYHLLKDVLGLD 180 LV PI ISAKVNGEPC TYIGPDGAGHYVKMVHNGIEYGDMQLI E+Y LLK+VLGL Sbjct: 151 LVAPIFKDISAKVNGEPCTTYIGPDGAGHYVKMVHNGIEYGDMQLISESYFLLKNVLGLS 210 Query: 181 AKELHSIFADWNSGELDSYLIEITKDIFAEYDEETGKPMVDVILDAAGQKGTGKWTSQSS 240 A+ELH++FADWN GELDSYLIEIT DIF + DEETGKP+VDVILD AGQKGTGKWTSQS+ Sbjct: 211 AEELHTVFADWNKGELDSYLIEITADIFTKKDEETGKPLVDVILDTAGQKGTGKWTSQSA 270 Query: 241 LDLGVPLSMITESVFSRFLSAMKEERVAASKVLSGPVTEPFQGDKAEFIENVRKALFASK 300 LDLGVPL +ITESVF+RFLSAMKEERV AS++L GP + FQG++ FIE+VRKAL+ SK Sbjct: 271 LDLGVPLPIITESVFARFLSAMKEERVHASRLLKGPEAKSFQGNREAFIESVRKALYMSK 330 Query: 301 IVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLLL 360 I SYAQGFAQ+R AS+EY+WDLKYG++A I+RGGCIIR++FLQ I +AY+ P LKNLLL Sbjct: 331 ICSYAQGFAQMRAASDEYSWDLKYGDIAMIFRGGCIIRAQFLQKIKEAYDREPGLKNLLL 390 Query: 361 DPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFSSALAYYDSYRTERLPANLLQAQRDYFG 420 DP+FK I ++YQ+A R+++S+AV G+PVP FS+AL+YYDSYRTE LPANLLQAQRDYFG Sbjct: 391 DPYFKEIAESYQAALREIISSAVQNGIPVPCFSAALSYYDSYRTETLPANLLQAQRDYFG 450 Query: 421 AHTFKRVDKEGVFHHNWL 438 AHT++R DKEG FH NW+ Sbjct: 451 AHTYQRTDKEGTFHTNWI 468 >Q5KXF7.1 Q5KXF7_GEOKA 6-phosphogluconate dehydrogenase, decarboxylating EC=1.1.1.44 Length = 469 Score = 696 bits (1795), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/438 (73%), Positives = 380/438 (86%) Query: 1 VYNRSPEKTHDLINEAEGKNLTGTFSIEEFVESLEVPRKILIMVQAGKATDATIEQLLPH 60 VYNRS EKT + + EA+GKN+ GT+SIEEFV +LE PRKIL+MV+AG TDATIEQL PH Sbjct: 31 VYNRSREKTDEFLQEAKGKNIVGTYSIEEFVNALEKPRKILLMVKAGAPTDATIEQLKPH 90 Query: 61 LDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFIGTGVSGGEEGALKGPSIMPGGQESAYK 120 L++GDI+IDGGN YF DT RRNKEL G FIGTGVSGGEEGALKGPSIMPGGQ+ A++ Sbjct: 91 LEKGDIVIDGGNTYFKDTQRRNKELAELGIHFIGTGVSGGEEGALKGPSIMPGGQKEAHE 150 Query: 121 LVEPILTAISAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLIGEAYHLLKDVLGLD 180 LV PI AI+AKV+GEPC TYIGPDGAGHYVKMVHNGIEYGDMQLI EAY LLK VLG+D Sbjct: 151 LVRPIFEAIAAKVDGEPCTTYIGPDGAGHYVKMVHNGIEYGDMQLIAEAYFLLKHVLGMD 210 Query: 181 AKELHSIFADWNSGELDSYLIEITKDIFAEYDEETGKPMVDVILDAAGQKGTGKWTSQSS 240 A ELH +FADWN GEL+SYLIEIT DIF + DEETGKP+VDVILD AGQKGTGKWTSQ++ Sbjct: 211 AAELHEVFADWNKGELNSYLIEITADIFTKIDEETGKPLVDVILDKAGQKGTGKWTSQNA 270 Query: 241 LDLGVPLSMITESVFSRFLSAMKEERVAASKVLSGPVTEPFQGDKAEFIENVRKALFASK 300 LDLGVPL +ITESVF+RFLSAMK+ERV ASKVL+GP +PF+GD+A FIE VR+AL+ SK Sbjct: 271 LDLGVPLPIITESVFARFLSAMKDERVKASKVLAGPAVKPFEGDRAHFIEAVRRALYMSK 330 Query: 301 IVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLLL 360 I SYAQGFAQ++ ASEEYNW+L+YG++A I+RGGCIIR++FLQ I +AY+ +P L NLLL Sbjct: 331 ICSYAQGFAQMKAASEEYNWNLRYGDIAMIFRGGCIIRAQFLQKIKEAYDRDPALSNLLL 390 Query: 361 DPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFSSALAYYDSYRTERLPANLLQAQRDYFG 420 DP+FK+I++ YQ A R++V+ A +G+PVPGF+SALAYYDSYRT LPANL+QAQRDYFG Sbjct: 391 DPYFKDIVERYQDALREIVATAAMRGIPVPGFASALAYYDSYRTAVLPANLIQAQRDYFG 450 Query: 421 AHTFKRVDKEGVFHHNWL 438 AHT++RVDKEG+FH WL Sbjct: 451 AHTYERVDKEGIFHTEWL 468 >P80859.4 6PGD2_BACSU 6-phosphogluconate dehydrogenase, decarboxylating 2 EC=1.1.1.44 Length = 469 Score = 694 bits (1792), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/438 (74%), Positives = 374/438 (85%) Query: 1 VYNRSPEKTHDLINEAEGKNLTGTFSIEEFVESLEVPRKILIMVQAGKATDATIEQLLPH 60 VYNRS KT + + EA+GKN+ GT+SIEEFV+SLE PRKIL+MV+AG ATDATI+ LLPH Sbjct: 31 VYNRSSSKTEEFLQEAKGKNVVGTYSIEEFVQSLETPRKILLMVKAGTATDATIQSLLPH 90 Query: 61 LDQGDIIIDGGNAYFPDTVRRNKELEAKGFRFIGTGVSGGEEGALKGPSIMPGGQESAYK 120 L++ DI+IDGGN Y+ DT RRNKEL G FIGTGVSGGEEGALKGPSIMPGGQ+ A++ Sbjct: 91 LEKDDILIDGGNTYYKDTQRRNKELAESGIHFIGTGVSGGEEGALKGPSIMPGGQKEAHE 150 Query: 121 LVEPILTAISAKVNGEPCCTYIGPDGAGHYVKMVHNGIEYGDMQLIGEAYHLLKDVLGLD 180 LV+PIL AISAKV+GEPC TYIGPDGAGHYVKMVHNGIEYGDMQLI E+Y +LK VLGL Sbjct: 151 LVKPILEAISAKVDGEPCTTYIGPDGAGHYVKMVHNGIEYGDMQLISESYFILKQVLGLS 210 Query: 181 AKELHSIFADWNSGELDSYLIEITKDIFAEYDEETGKPMVDVILDAAGQKGTGKWTSQSS 240 A ELH +FA+WN GELDSYLIEIT DIF + DEETGKP+VDVILD AGQKGTGKWTSQS+ Sbjct: 211 ADELHEVFAEWNKGELDSYLIEITADIFTKKDEETGKPLVDVILDKAGQKGTGKWTSQSA 270 Query: 241 LDLGVPLSMITESVFSRFLSAMKEERVAASKVLSGPVTEPFQGDKAEFIENVRKALFASK 300 LDLGVPL +ITESVF+RF+SAMKEERV AS +LSGP +P +K E IE VRKALF SK Sbjct: 271 LDLGVPLPIITESVFARFISAMKEERVKASGLLSGPEVKPVTENKEELIEAVRKALFMSK 330 Query: 301 IVSYAQGFAQLRVASEEYNWDLKYGNLAKIWRGGCIIRSRFLQNITDAYENNPELKNLLL 360 I SYAQGFAQ++ ASEEYNWDLKYG +A I+RGGCIIR+ FLQ I +AY+ PEL NLLL Sbjct: 331 ICSYAQGFAQMKAASEEYNWDLKYGEIAMIFRGGCIIRAAFLQKIKEAYDREPELDNLLL 390 Query: 361 DPFFKNIMDTYQSAWRQVVSAAVAQGVPVPGFSSALAYYDSYRTERLPANLLQAQRDYFG 420 D +FKNI+++YQ A RQV+S AVAQGVPVP FSSALAYYDSYRT LPANL+QAQRDYFG Sbjct: 391 DSYFKNIVESYQGALRQVISLAVAQGVPVPSFSSALAYYDSYRTAVLPANLIQAQRDYFG 450 Query: 421 AHTFKRVDKEGVFHHNWL 438 AHT++R DKEG+FH W+ Sbjct: 451 AHTYERTDKEGIFHTEWM 468 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,746,715,560 Number of extensions: 120862747 Number of successful extensions: 331052 Number of sequences better than 1.0e-05: 2199 Number of HSP's gapped: 327019 Number of HSP's successfully gapped: 2231 Length of query: 440 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 301 Effective length of database: 1,751,175,118 Effective search space: 527103710518 Effective search space used: 527103710518 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)