BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0041.p (447 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DIS1.1 B1DIS1_9BACL GerA spore germination protein 489 e-136 B3K634.1 B3K634_9BACI GerA spore germination protein 236 3e-60 B1DM29.1 B1DM29_9BACL GerA spore germination protein 204 1e-50 Q8EMS3.1 Q8EMS3_OCEIH Spore germination protein 199 5e-49 B3K7T2.1 B3K7T2_9BACI GerA spore germination protein 182 6e-44 >B1DIS1.1 B1DIS1_9BACL GerA spore germination protein Length = 400 Score = 489 bits (1259), Expect = e-136, Method: Compositional matrix adjust. Identities = 244/406 (60%), Positives = 300/406 (73%), Gaps = 16/406 (3%) Query: 51 TFTMMNQLAPGEELSSLLHSNSW---------AEDTSSWKVSQYSDALLQGRLVLFLGGR 101 T ++M+Q+ ++ N W + +++ + + +A+LQG L+LF Sbjct: 2 TLSVMSQIGDSQD-------NKWKWEFLNGKEGDASATASLEKLCNAVLQGMLILFGKEG 54 Query: 102 QSVVVDPVAPELSRSVSTTNSENPLLSAFDAFTEDIDINIGLLRKKIVSDRLVIETRTSG 161 Q V++ A +SR V NSENPL SAFDAFTEDIDINIG+LRKK++SD+LVIE R G Sbjct: 55 QVAVIESEAQNISRPVMIPNSENPLQSAFDAFTEDIDINIGILRKKMISDQLVIECRQIG 114 Query: 162 TKSTKKLAVVYLEGAAQPKVVESIKKKLEENRGMELTTVRDLTRILGHPQFTLTPTYISS 221 T+S KKLA+ Y+EG +P+V+ESI+KKL+ENR ELTT+RDLTRILGHP+F LTPTY SS Sbjct: 115 TQSVKKLAIAYMEGVTRPEVIESIRKKLDENRRQELTTIRDLTRILGHPKFALTPTYTSS 174 Query: 222 EMPNETMQNMLNGKVVILIDQFSFAFAFPAIVADLWSTTMDVNYPQLFQNFLRFLRIGAL 281 E+P ETMQN+ NGKVVIL+DQFSFAFAFPAIV DLWST++D YP FQ FLR +R A+ Sbjct: 175 ELPGETMQNIQNGKVVILLDQFSFAFAFPAIVTDLWSTSLDTQYPLPFQMFLRMIRGMAM 234 Query: 282 ILAITLPGLYVVLNSVNPELLRIQLAITVAKSREGVPYPSXXXXXXXXXXXXXXXXATIR 341 +LAITLPGLYVVLNSVNPELLRIQLAI VA++R+GVPYPS ATIR Sbjct: 235 LLAITLPGLYVVLNSVNPELLRIQLAIAVAENRQGVPYPSLIEALLVMLLLEMIIEATIR 294 Query: 342 LPKNIGPTMTMIGGILLGQAIVQAKXXXXXXXXXXXXXXXXXFALTSYMNASGIRIYKYV 401 LPKNIGPT+TMIGGILLGQAI+QAK FALTSYMN+ G+R+YKYV Sbjct: 295 LPKNIGPTITMIGGILLGQAIIQAKLVSNLLIIILVASAIANFALTSYMNSVGVRLYKYV 354 Query: 402 VLLASAFFGIWGLEAAIIWLVLYFASLTISTVPYLSFSVKGEFPDE 447 VLLAS+FFGIWG+E A+IWL+LYF+SLT +VPYLSFS+KG+ DE Sbjct: 355 VLLASSFFGIWGIEVAMIWLMLYFSSLTNCSVPYLSFSLKGKTSDE 400 >B3K634.1 B3K634_9BACI GerA spore germination protein Length = 460 Score = 236 bits (602), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 136/444 (30%), Positives = 236/444 (53%), Gaps = 20/444 (4%) Query: 7 DTFERMEQQLQEHFHKSNDFARKQFKAGEV-CSAYYLSSLVDI-ESTFTMMNQ------- 57 D + + L E+F + D+ + + + L SLVDI +S ++ Q Sbjct: 14 DADMNLNESLAEYFKANADYFNVEIRLLQYHVQVIGLDSLVDIPQSIASLQTQTSNLTET 73 Query: 58 -LAPGEELSSLLHSNSWAEDTSSWKVSQYSDALLQGRLVLF--LGGRQSVVVDPVAPELS 114 LAP + L + K+ +++G L++ G S V+ P++ ++ Sbjct: 74 DLAPADLLK-------IVGELVEPKLEVVVPDIMKGHLIILEPASGACSAVL-PISKTVT 125 Query: 115 RSVSTTNSENPLLSAFDAFTEDIDINIGLLRKKIVSDRLVIETRTSGTKSTKKLAVVYLE 174 RSV++ +EN L + AF EDI+ NIG+LRK L +ET T G K L ++Y + Sbjct: 126 RSVTSPETENTLYGSSSAFLEDINTNIGILRKHCTGSSLQVETYTVGKSHPKSLLLIYQK 185 Query: 175 GAAQPKVVESIKKKLEENRGMELTTVRDLTRILGHPQFTLTPTYISSEMPNETMQNMLNG 234 +P++++SI+ K++ + ++ ++ L ++LG ++L + +E+P ++ G Sbjct: 186 DKIKPRLLQSIQAKIKADLDQDVHHIQQLEKMLGVNTWSLVTNFNMTELPQNAAHSLQQG 245 Query: 235 KVVILIDQFSFAFAFPAIVADLWSTTMDVNYPQLFQNFLRFLRIGALILAITLPGLYVVL 294 K V+++D++ FA P++V D++ D NYP F +R LR+ +++A +PGLYV L Sbjct: 246 KAVLMLDRYPFAIIIPSLVMDMFCMKDDHNYPLPFMYLIRILRVVGVLIATIIPGLYVAL 305 Query: 295 NSVNPELLRIQLAITVAKSREGVPYPSXXXXXXXXXXXXXXXXATIRLPKNIGPTMTMIG 354 SVNPE+LR+QLA+++A SR+ VPYP+ A++RLPK++GPT+TM+G Sbjct: 306 VSVNPEVLRLQLALSIANSRQEVPYPAFVETLLLLIVLELILEASVRLPKSVGPTITMVG 365 Query: 355 GILLGQAIVQAKXXXXXXXXXXXXXXXXXFALTSYMNASGIRIYKYVVLLASAFFGIWGL 414 GI+LGQA V AK + + N+ +R++KY++++ SA +G+ GL Sbjct: 366 GIILGQAAVSAKLVSNLLIIVLAGTTIASSTVVGFQNSVSVRVFKYMLIILSAIYGMLGL 425 Query: 415 EAAIIWLVLYFASLTISTVPYLSF 438 A I+ + Y +PYLS Sbjct: 426 LAGIVVICAYMGHQKSMGIPYLSL 449 >B1DM29.1 B1DM29_9BACL GerA spore germination protein Length = 442 Score = 204 bits (519), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 135/434 (31%), Positives = 222/434 (51%), Gaps = 5/434 (1%) Query: 15 QLQEHFHKSNDFARKQFK-AGEVCSAYYLSSLVDIESTFTMMNQLAPGEELSSLLHSNSW 73 ++ HF D+ ++ + A E + L SL++ T M+ +S L + + Sbjct: 6 EVNSHFSSYPDYVNQKLQFASETFTFAGLKSLINFPQTIYMVRDPLLTATISKLELIDVF 65 Query: 74 AEDTSSWKVSQYS--DALLQGRLVLFLGGRQ-SVVVDPVAPELSRSVSTTNSENPLLSAF 130 + V S ++ G LV +V + PV SR +S EN + Sbjct: 66 ISLGQTITVQPDSIISQIMDGMLVAVHDTSGIAVSIYPVPQPNSRPISLPTMENSIGGPT 125 Query: 131 DAFTEDIDINIGLLRKKIVSDRLVIETRTSGTKSTKKLAVVYLEGAAQPKVVESIKKKLE 190 AF E+++ NI LLR + L I+ G ++ + YLE P +++++ +KLE Sbjct: 126 SAFNENLESNIVLLRSHLNCPDLQIKAYDFGGHVKSRVILCYLEQEVDPILLDNMTQKLE 185 Query: 191 ENRGMELTTVRDLTRILGHPQFTLTPTYISSEMPNETMQNMLNGKVVILIDQFSFAFAFP 250 ++ E++ +++L IL +++ Y++ E+P ++ + NGK V+ I++ F+ P Sbjct: 186 AHQNTEISNMQELAHILKFSKYSFVTHYMTYELPQNAVRALKNGKAVLFIERVPFSIVLP 245 Query: 251 AIVADLWSTTMDVNYPQLFQNFLRFLRIGALILAITLPGLYVVLNSVNPELLRIQLAITV 310 +I +DL+ + D+N+P ++ LR LR +L + LPGLYV L SVNP++LR +LA ++ Sbjct: 246 SIFSDLFISEDDLNHPPIYSWLLRSLRFIGTLLTLILPGLYVALVSVNPDVLRFELAHSI 305 Query: 311 AKSREGVPYPSXXXXXXXXXXXXXXXXATIRLPKNIGPTMTMIGGILLGQAIVQAKXXXX 370 A+SR VPYP+ A IRLP +IGPT+TM+GGI+LGQA+V AK Sbjct: 306 ARSRLDVPYPAIIEALLLLFILELIMEAIIRLPGSIGPTITMVGGIVLGQAVVDAKLVSN 365 Query: 371 XXXXXXXXXXXXXFALTSYMNASGIRIYKYVVLLASAFFGIWGLEAAIIWLVLYFASLTI 430 A+ + N +R++KY++L SAF G+ GL + +I L Y A + Sbjct: 366 LLIIILAATTIANAAVVGFQNTYALRLFKYLLLFMSAFLGVLGLLSGLIVLCAYVAGIRT 425 Query: 431 STVPYLS-FSVKGE 443 VPYL F KGE Sbjct: 426 LGVPYLQLFRAKGE 439 >Q8EMS3.1 Q8EMS3_OCEIH Spore germination protein Length = 443 Score = 199 bits (506), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 135/405 (33%), Positives = 223/405 (55%), Gaps = 8/405 (1%) Query: 42 LSSLVDIESTFTMMNQLAPGEELSSLLHSNSWAEDTSSWKVSQYSD---ALLQGRLVLFL 98 SLVD +T+ M+ L EE+ + +N AE S+WK + ++ L +G+++++ Sbjct: 37 FESLVDFSATWKMIETLIQNEEIP-VQETNVHAEQ-STWKKMRSTEQFKTLYEGKVIIWN 94 Query: 99 GGRQ-SVVVDPVAPELSRSVSTTNSENPLLSAFDAFTEDIDINIGLLRKKIVSDRLVIET 157 ++ S++VDPV+ L RS+ +E+ + A DAFTE + NIGLLR K S L + + Sbjct: 95 PTKEVSLIVDPVSRPLYRSIDAPVNESIIYGARDAFTESLQQNIGLLRSKYKSKELEVLS 154 Query: 158 RTSGTKSTKKLAVVYLEGAAQPKVVESIKKKLEENRGMELTTVRDLTRILGHPQFTLTPT 217 + G ++ +K + + L+G K+V+ IKKK++E E+ V+D+ LG +F L Sbjct: 155 TSHGQRN-RKFSFIVLKGTTNEKMVQKIKKKIDEFPN-EIKQVKDVHVALGFSKFDLVTR 212 Query: 218 YISSEMPNETMQNMLNGKVVILIDQFSFAFAFPAIVADLWSTTMDVNYPQLFQNFLRFLR 277 S+E+P M+ + GKVV +D + F V D++ D NY +F L LR Sbjct: 213 VSSTELPENAMEYIEQGKVVFFMDGYPFGLVLFNTVFDIFKQPSDSNYLSVFVYALYALR 272 Query: 278 IGALILAITLPGLYVVLNSVNPELLRIQLAITVAKSREGVPYPSXXXXXXXXXXXXXXXX 337 I ++L++ LPGLYV L ++NPE+L+IQLA+++ +SR+ VPYP+ Sbjct: 273 IVGIMLSLLLPGLYVALITINPEMLKIQLALSITESRQFVPYPAFIEIMFMLLLIELITE 332 Query: 338 ATIRLPKNIGPTMTMIGGILLGQAIVQAKXXXXXXXXXXXXXXXXXFALTSYMNASGIRI 397 A +RLP +I T+ ++GGI+LGQAIV+A + ++ N +R+ Sbjct: 333 AAVRLPLSISNTIGIVGGIILGQAIVEASLVSGVLIIVIAATTIANGIIVNFENGITLRL 392 Query: 398 YKYVVLLASAFFGIWGLEAAIIWLVLYFASLTISTVPYLSFSVKG 442 KY +++ SA FGI G +++ L Y A +T VPY+ + +G Sbjct: 393 MKYFIVIFSALFGILGHVISLLILCSYIARITNFGVPYVRWKKEG 437 >B3K7T2.1 B3K7T2_9BACI GerA spore germination protein Length = 453 Score = 182 bits (462), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 122/437 (27%), Positives = 218/437 (49%), Gaps = 19/437 (4%) Query: 10 ERMEQQLQEHFHKSNDFARKQFK-AGEVCSAYYLSSLVD-------IESTFTMMNQLAPG 61 ++ + L+E ++ D +++ K E YL S+ D + + F +N L Sbjct: 10 DQAKNCLKEQLNRIGDIEQRELKNEDEHALLVYLKSMCDPAMINDNLINRFYELNSLDLY 69 Query: 62 EELSSLLHSNSWAEDTSSWKVSQYSDALLQGRLVLFLGGRQSVVVDPVAPELSRSVSTTN 121 E+ S++ +D + +L+G + +F+ R +V++ + + + Sbjct: 70 EQFIRSFPSSTEPKDEQ-----ELLRNILRGCVAIFI--RDTVLLFDAVLVNASFIQPAS 122 Query: 122 SENPLLSAFDAFTEDIDINIGLLRKKIVSDRLVIETRTSGTKSTKKLAVVYLEGAAQPKV 181 +EN + D+FTE+I++N+ L+R + + L E T G S ++ ++Y + V Sbjct: 123 TENVIQGPDDSFTENIEVNLNLVRHRYQTTDLKTEFMTVGKISQTRIIIMYDALKVEETV 182 Query: 182 VESIKKKLEENRGMELTTVRDLTRILGHPQFTLTPTYISSEMPNETMQNMLNGKVVILID 241 ++ +KK+L E + + + ++ R HP+F L PT + +E P+ T+ N+ GK+ +L+D Sbjct: 183 LQELKKRLSELKADIVQSASEIERHTMHPRFRLYPTLMLTERPDRTVLNISQGKIAVLMD 242 Query: 242 QFSFAFAFPAIVADLWSTTMD--VNYPQLFQNFLRFLRIGALILAITLPGLYVVLNSVNP 299 +A PAI D + T MD + P + FL+ +R AL + + +P LYV S NP Sbjct: 243 STGYALLLPAIFNDFF-TAMDDKIQLPPIGW-FLKAIRYIALFVTVLMPSLYVAFTSYNP 300 Query: 300 ELLRIQLAITVAKSREGVPYPSXXXXXXXXXXXXXXXXATIRLPKNIGPTMTMIGGILLG 359 E+LR+Q+ + +A SR VPYPS A++RLPK IG T T +GG++LG Sbjct: 301 EILRMQVTLLIAGSRATVPYPSFFEVIFMLLAMEFLTEASVRLPKAIGQTATTVGGLILG 360 Query: 360 QAIVQAKXXXXXXXXXXXXXXXXXFALTSYMNASGIRIYKYVVLLASAFFGIWGLEAAII 419 A +A F + M +SGIR+ KY+ ++ + FFG+ G+ ++ Sbjct: 361 TAATEAGLVSNIMIILVSAVAITNFVIPITMMSSGIRVTKYLFIVLATFFGLVGIVLGVV 420 Query: 420 WLVLYFASLTISTVPYL 436 +++Y SL PYL Sbjct: 421 AMIMYLTSLRSFGKPYL 437 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,238,550,548 Number of extensions: 80374760 Number of successful extensions: 229131 Number of sequences better than 1.0e-05: 1067 Number of HSP's gapped: 226557 Number of HSP's successfully gapped: 1067 Length of query: 447 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 308 Effective length of database: 1,751,175,118 Effective search space: 539361936344 Effective search space used: 539361936344 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)