BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0040.p (275 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DIS0.1 B1DIS0_9BACL Spore germination protein 353 1e-95 B3K635.1 B3K635_9BACI Spore germination protein 128 6e-28 B1DM30.1 B1DM30_9BACL Spore germination protein 100 3e-19 Q8EMS4.1 Q8EMS4_OCEIH Putative uncharacterized protein OB2767 64 1e-08 B1DHB7.1 B1DHB7_9BACL Spore germination protein 60 3e-07 >B1DIS0.1 B1DIS0_9BACL Spore germination protein Length = 356 Score = 353 bits (905), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 168/257 (65%), Positives = 216/257 (84%) Query: 1 MNKIQTLTLFVLLHLTSIFAFFPERIIAATSKGHWMAITFLFAVELGILWVYLKALSLAP 60 MNK++T+ +F LLHL SIFA FPER++++ +GHWMA+ LF EL LW YLKALS P Sbjct: 1 MNKLKTIIMFFLLHLASIFAIFPERMMSSAPRGHWMAVLLLFLAELLALWFYLKALSYFP 60 Query: 61 GKTVIDICREAMGTWITRLIMLPFMAFLIIELVMLTYYQAVEIKVVLLQRTPVAAISALF 120 ++++++CR A+G+W TRLI+LPF+ FLIIELV+LTYY+++EIK VLLQ+TP++A SALF Sbjct: 61 ERSIVEVCRAAVGSWGTRLIILPFLCFLIIELVLLTYYESIEIKTVLLQKTPISATSALF 120 Query: 121 VMLCMYGAWKGLVVMVRASLGWIFLFMPFILFSMFISIRNFRFDYIFPLWDADLSFLFRS 180 V +C+Y AWKGL VM+RAS+GW L MPF +FSM ISI+NF F YIFP+WD+ + FLF Sbjct: 121 VAICLYAAWKGLTVMIRASIGWFLLCMPFFIFSMLISIKNFEFRYIFPMWDSGMHFLFHP 180 Query: 181 DFYVGTVIFAGFLFLGMTATKTKIGFGKAAAAVGIVYLFAIASVYIPLLIFGQETVLHLQ 240 DFYVGTVIFAG+LFLGMT + +IGFG A+AA+G+V+L A+ASVYIPLLIFGQET +H++ Sbjct: 181 DFYVGTVIFAGYLFLGMTTSHFRIGFGLASAAIGLVFLCALASVYIPLLIFGQETAVHME 240 Query: 241 YPMLMASDTIDLEWVVF 257 YPMLMASDT+DLEWVVF Sbjct: 241 YPMLMASDTVDLEWVVF 257 >B3K635.1 B3K635_9BACI Spore germination protein Length = 364 Score = 128 bits (321), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 85/260 (32%), Positives = 138/260 (53%), Gaps = 6/260 (2%) Query: 2 NKIQTLTLFVLLHLTSIFAFFPERIIAATSKGHWMAITFLFAVELGILWVYLKALSLAPG 61 N Q L+V+ H+ IF +P + + GHW+ I+ +A+ L++Y+K L A Sbjct: 4 NGYQATILYVVCHMGLIFFLYPSDMFSGMDMGHWIGISISYALHAAALYMYIKGLKWAAQ 63 Query: 62 KTVIDICREAMGTWITRLIMLP-FMAFLIIELVMLTYYQAVEIKVVLLQRTPVAAISALF 120 + V+D+ R G ++ +++LP F F I +V L Y + + +V L TP+ A+ L Sbjct: 64 RNVVDMFRST-GKYLAWILLLPAFFYFGIAIIVTLRAYSEM-LTLVFLSSTPLWAVQLLL 121 Query: 121 VMLCMYGAWKGLVVMVRASLGWIFLFMPFILFSMFISIRNFRFDYIFPLWDADLSFLF-- 178 + + + AW+G+ M R S+ + LFM ILF + + +N + Y+ PL D + SF F Sbjct: 122 IAIGLLMAWQGMPSMARTSVLLVVLFMFPILFVLCLGFQNVDWYYLLPLIDREQSFNFIA 181 Query: 179 RSDFYVGTVIFA-GFLFLGMTATKTKIGFGKAAAAVGIVYLFAIASVYIPLLIFGQETVL 237 + DF V ++A G FLG+ I K ++ SVY+PLL FGQ T Sbjct: 182 KPDFLVSLFVYAGGSFFLGLLPPSIHISLKKMMLGCLLLLPMFFLSVYLPLLTFGQSTAE 241 Query: 238 HLQYPMLMASDTIDLEWVVF 257 Q+PMLM DT+++ W++F Sbjct: 242 LYQFPMLMTIDTVNITWLLF 261 >B1DM30.1 B1DM30_9BACL Spore germination protein Length = 364 Score = 99.8 bits (247), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 80/267 (29%), Positives = 139/267 (52%), Gaps = 19/267 (7%) Query: 2 NKIQTLTLFVLLHLTSIFAFFPERIIAATSKGHWMAITFLFAVELGILWVYLKALSLAPG 61 +Q T +++ HL IF +P +I +T++ HWM + F + ++++YLK LS G Sbjct: 3 KNLQVSTTYIMTHLGLIFFLYPIDVIDSTNEAHWMPVLMGFLFHISVIFIYLKGLSYFKG 62 Query: 62 KTVIDIC--REAMGTWITRLIMLPFMAFLIIELVMLTYYQAVEIKVVLLQRTPVAAISAL 119 + +++I + W+ L +M+++I+ L + + + + I + L TP+ + L Sbjct: 63 QNIVNILMKKNKFLAWVVLFPALLYMSWIIV-LAVRAFSEVLSIAI--LSNTPLWILMLL 119 Query: 120 FVM----LCMYGAWKGLVVMVRASLGWIFLFMPFILFSMFISIRNFRFDYIFPLWDADLS 175 F++ + ++G GL +R S+ FLF+ ++F M S +N + Y FPLW + Sbjct: 120 FLVVPGFMILHGQIGGL---LRLSVLLSFLFVVPVVFVMATSFQNIDWHYFFPLWPGQGA 176 Query: 176 FLF--RSDFYVGTVIF-AGFLFLGMTATKTKIGFGKAAAAVGIVYLFA--IASVYIPLLI 230 F F S FY + GFL LG T + F A +G + L + +VY+PLL Sbjct: 177 FTFMAHSSFYKSLFAYTGGFLLLGFLPTYAE--FKSRAIYLGSLILLPAYLIAVYVPLLT 234 Query: 231 FGQETVLHLQYPMLMASDTIDLEWVVF 257 G+ET LQ+P L DTI++ W++F Sbjct: 235 LGEETTRQLQFPYLFIVDTIEINWLMF 261 >Q8EMS4.1 Q8EMS4_OCEIH Putative uncharacterized protein OB2767 Length = 358 Score = 64.3 bits (155), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 65/267 (24%), Positives = 118/267 (44%), Gaps = 19/267 (7%) Query: 1 MNKIQTLTLFVLLHLTSIFAFFPERIIAATSKGHWMAIT---FLFAVELGILWVYLKALS 57 +N + +T F+++++ +F +P I+ GHW+ + F + V LG V+L +S Sbjct: 3 INNLLVITYFLIVNIGLMFLLYPTLILDTVENGHWLVVLLHGFSYMVLLG---VFLYGMS 59 Query: 58 LAPGKTVIDICREAMGTWITR-LIMLPFMAFLIIELVMLTYYQAVEIKVVLLQRTPVAAI 116 +++ + WI L+++P F+I ++ A V+ L +TP + Sbjct: 60 KLKQPNILEYVPK--NRWIVSILVLVPISLFIIGTVITAIRTYAEFTTVLFLNQTPFLFV 117 Query: 117 SALFVMLCMYGAWKGLVVMVRASLGWIFLFMPFILFSMFISIRNFRFDYIFPLWDADLSF 176 LF++ Y + + R S I I+ F+S N + Y FP + F Sbjct: 118 MLLFIIAATYIGLGSIGSIFRTSFLIIATSSLIIVVIFFLSFFNAEWYYFFPAKVSSFDF 177 Query: 177 LFRSDFYVGTVIFA-GFLFLGMTATKT-----KIGFGKAAAAVGIVYLFAIASVYIPLLI 230 L ++ F G +I FLFL + K F + + I++ S Y P+L+ Sbjct: 178 LIQTPFITGFLILTPTFLFLAFLPKRRGDYNFKTIFWSSMILIPIIF----VSAYSPILV 233 Query: 231 FGQETVLHLQYPMLMASDTIDLEWVVF 257 FG E + +P + T+D+EW +F Sbjct: 234 FGYEVAKNFTFPYIEMLATVDIEWSIF 260 >B1DHB7.1 B1DHB7_9BACL Spore germination protein Length = 374 Score = 59.7 bits (143), Expect = 3e-07, Method: Compositional matrix adjust. Identities = 49/240 (20%), Positives = 109/240 (45%), Gaps = 5/240 (2%) Query: 22 FPERIIAATSKGHWMAITFLFAVELGILWVYLKALSLAPGKTVIDICREAMGTWITRLIM 81 +P I+ +T +W+ IT A + W+Y + L G+ ++ ++G + T LI Sbjct: 35 YPSFIVRSTEGAYWIPITIWVACAIFSSWIYSRLLIRLNGELLLRRIHASIGLFWTCLIC 94 Query: 82 LPFMAFLIIELVMLTYYQAVEIKVVLLQRTPVAAISALFVMLCMYGAWKGLVVMVRASLG 141 LP + F + LV++ + I + +L TP++ ++ + + G++ +VRA+ Sbjct: 95 LPVLLFFLTALVIMLRAYSEVITMTMLPTTPISFLNGMLIAPAALAL-AGIMPIVRAARV 153 Query: 142 WIFLFMPFILFSMFISIRNFRFDYIFPLWDADLSFLFRSDFYVGTVIFAGFLFLGMTATK 201 + L + + + + + + + P + F + FY G+ ++ GF+ Sbjct: 154 FFLLTLLLTVLLLIVGLSDVHWTLGAPWIRTNGDFFYDKRFYAGSFMWMGFVITSFIGPY 213 Query: 202 TKIGFGKAAAAVGIVYLFAI----ASVYIPLLIFGQETVLHLQYPMLMASDTIDLEWVVF 257 T+ A ++ + L + +Y+P+L FG++ L P + D++ W++F Sbjct: 214 TRQSARSAISSYTVAILCTLPLIAGYIYLPVLTFGRDLSKRLTVPFISKMDSVSHYWILF 273 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.336 0.146 0.466 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,462,680,707 Number of extensions: 54672028 Number of successful extensions: 251103 Number of sequences better than 1.0e-05: 70 Number of HSP's gapped: 251266 Number of HSP's successfully gapped: 70 Length of query: 275 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 141 Effective length of database: 1,798,280,193 Effective search space: 253557507213 Effective search space used: 253557507213 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.7 bits) S2: 130 (54.7 bits)