BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0038.p (354 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DIR9.1 B1DIR9_9BACL Germination protein, Ger(X)C family 523 e-146 B3K636.1 B3K636_9BACI Germination protein, Ger(X)C family 204 1e-50 B1DM31.1 B1DM31_9BACL Germination protein, Ger(X)C family 196 4e-48 Q8EMS5.1 Q8EMS5_OCEIH Putative uncharacterized protein OB2766 128 8e-28 B1DHB8.1 B1DHB8_9BACL Putative uncharacterized protein 89 6e-16 >B1DIR9.1 B1DIR9_9BACL Germination protein, Ger(X)C family Length = 359 Score = 523 bits (1347), Expect = e-146, Method: Compositional matrix adjust. Identities = 245/354 (69%), Positives = 302/354 (85%), Gaps = 7/354 (1%) Query: 1 VMLVMSLLLSGCWDTKDINKEYMPAVMGIGKGKTEKYRVILQVPNATGRTLFLEKEAKSI 60 V+L M +LL GCWDTKDINK+Y+P VMGIGKG+T KYR+ILQVP+ GRT FL+KEA S+ Sbjct: 11 VVLCM-MLLGGCWDTKDINKQYLPVVMGIGKGRTAKYRIILQVPSLKGRTQFLDKEAGSL 69 Query: 61 TKAVNLIQTDAEKDISLVHLRLLLIDKELAEEGISNIVDFAVRANDISIKGMMAVVDGDF 120 +KA++LI+TD+EK I+LVHLRLLL D+ELA EGI +++DFAVRANDISIKGM+AVVDGDF Sbjct: 70 SKAIDLIRTDSEKSINLVHLRLLLFDQELAREGIHDLIDFAVRANDISIKGMIAVVDGDF 129 Query: 121 EKTLYHEISPTPEVSSYDFFSEEAGWTPNQSIIRIWEAYRSLNSYSEDMAIPMLKNGEHT 180 ++TLYH ISPTPE+SSYDFFSEEAGWTPNQSI+RIWEAYRS NSYSEDMAIPMLK+G+ T Sbjct: 130 KQTLYHVISPTPELSSYDFFSEEAGWTPNQSIVRIWEAYRSQNSYSEDMAIPMLKSGKRT 189 Query: 181 LFVFKGTAVMKEDRMIGRLDQEETLLYNLFKGKYTGGTIELARNTSVLVENAKIKHHREW 240 L+ F+GTA+M EDRM+G+L+QEETLLYNLFKGKYTGGTIE+A NTSVL++ AKIKHH W Sbjct: 190 LYTFQGTAIMLEDRMVGKLNQEETLLYNLFKGKYTGGTIEVAENTSVLIQKAKIKHHCRW 249 Query: 241 SKEGPSLTSDITLNVVIAESPKGVSNDQIEKEVWKQLDRKFNETLKKITGYHSDVLGVGL 300 + EGP+L +DI L+VV++ESP+G S QIE+E+ +QL+R FN+T KI YHSDVLGVGL Sbjct: 250 TNEGPNLLTDINLDVVVSESPRGKSKQQIEEEIKQQLERNFNKTADKIHKYHSDVLGVGL 309 Query: 301 LYRPLLSETEITAWKSRWFPALKQQINVKVNVLNEIYFKNTDQDRNQGGLLKSR 354 ++RP LS+ I W+S+W+P L+Q+INV VNV NEIY K + G+ KSR Sbjct: 310 IFRPKLSDANIKDWRSKWYPKLEQKINVHVNVQNEIYLK------EEAGVKKSR 357 >B3K636.1 B3K636_9BACI Germination protein, Ger(X)C family Length = 360 Score = 204 bits (518), Expect = 1e-50, Method: Compositional matrix adjust. Identities = 104/339 (30%), Positives = 192/339 (56%), Gaps = 8/339 (2%) Query: 8 LLSGCWDTKDINKEYMPAVMGIGKGKTEKYRVILQVP--NATGRTLFLEKEAKSITKAVN 65 LL+GCWD KDIN +P +MGI + +Y+V L +P N T + +I + ++ Sbjct: 23 LLAGCWDNKDINHRSLPVIMGISLTEENQYKVFLDIPVTNETSTVNIVSDTGNTINEIID 82 Query: 66 LIQTDAEKDISLVHLRLLLIDKELAEEGISNIVDFAVRANDISIKGMMAVVDGDFEKTLY 125 + + E + L+HL+++++DK A +G+ +I+ R+ DIS K ++A+ D + ++ + Sbjct: 83 HMSMNMETQVDLLHLKIVIVDKNFASKGMEDIISAFNRSRDISSKTLLAICDEELDQ-FF 141 Query: 126 HEISPTPEVSS---YDFFSEEAGWTPNQSIIRIWEAYRSLNSYSEDMAIPMLKNGEHTLF 182 ++ E S YDFF + AGW P + RIW+ +RS++SY+ D+ +P++++G T Sbjct: 142 SKMQEKSEYSGSIVYDFFEKNAGWNPQLADTRIWQMFRSIHSYTHDVIVPIIRSGRSTSI 201 Query: 183 VFKGTAVMKEDRMIGRLDQEETLLYNLFKGKYTGGTIELARNTSVLVENAKIKHHREWSK 242 G+A++K RM GR+ +ETL+ N F GK G +E+ + +V + + ++ HR W K Sbjct: 202 ECVGSAIIKNGRMTGRIGPDETLVANAFYGKSAFGKVEVMNSATVQIISNRLT-HRSWMK 260 Query: 243 EG-PSLTSDITLNVVIAESPKGVSNDQIEKEVWKQLDRKFNETLKKITGYHSDVLGVGLL 301 +G P L S + L V I +S + +QI ++ + L + N+ K+ +D+L +G Sbjct: 261 DGRPYLRSHLRLKVTILDSRGHPTEEQINGKLEELLTTRLNQMFKQTQKEQADILALGQH 320 Query: 302 YRPLLSETEITAWKSRWFPALKQQINVKVNVLNEIYFKN 340 +R L+ ++ W++ ++P L +++V + N KN Sbjct: 321 FRNHLTREQLQDWRTTYYPDLDFKLSVTTVIENRGNLKN 359 >B1DM31.1 B1DM31_9BACL Germination protein, Ger(X)C family Length = 351 Score = 196 bits (497), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 103/337 (30%), Positives = 182/337 (54%), Gaps = 8/337 (2%) Query: 4 VMSLLLSGCWDTKDINKEYMPAVMGIGKGKTEKYRVILQVPNATGRT---LFLEKEAKSI 60 V +LL GCWD KDIN +P MGI + + Y V L +P + + + + SI Sbjct: 10 VFVILLGGCWDNKDINHRALPIAMGISYQQGD-YEVFLDIPKVSYKNEGIQVISAKGNSI 68 Query: 61 TKAVNLIQTDAEKDISLVHLRLLLIDKELAEEGISNIVDFAVRANDISIKGMMAVVDGDF 120 + AVN I + E + L+HL+++++DK A G+S I +R+ +S K M + D Sbjct: 69 SAAVNNISMNLENQVDLLHLKVIIVDKAFATRGLSEITSNIIRSRHVSPKTMFVISDEPL 128 Query: 121 EKTLYHEISPTPEVSS---YDFFSEEAGWTPNQSIIRIWEAYRSLNSYSEDMAIPMLKNG 177 ++ + I+ T + YDFF + +GW P + +W+ +RS++SY+ D+ +P++K+G Sbjct: 129 DQ-FFKGINNTSNGTGNEIYDFFQKNSGWNPEIAHTPVWKVFRSIHSYTNDVVLPIIKSG 187 Query: 178 EHTLFVFKGTAVMKEDRMIGRLDQEETLLYNLFKGKYTGGTIELARNTSVLVENAKIKHH 237 + T G+ ++K +M+ RL+ E+TLLYN+F G G IE+ +V + ++ I + Sbjct: 188 DSTAIQSMGSGIIKNGKMVSRLNNEQTLLYNVFDGNSLDGKIEVMGKATVQIVSSHIHNE 247 Query: 238 REWSKEGPSLTSDITLNVVIAESPKGVSNDQIEKEVWKQLDRKFNETLKKITGYHSDVLG 297 ++ P L S+++L V+I + + + I+ E+ L N+ KI +D+ G Sbjct: 248 SKFVNNLPVLNSELSLKVIILDVLGEPTMEVIKMELQNLLQHILNQIFLKIERVKADIFG 307 Query: 298 VGLLYRPLLSETEITAWKSRWFPALKQQINVKVNVLN 334 +G YR L TE+ W++++ P K + VK + N Sbjct: 308 IGQHYRDDLPRTELAQWRTKYLPEAKINLEVKTTIRN 344 >Q8EMS5.1 Q8EMS5_OCEIH Putative uncharacterized protein OB2766 Length = 368 Score = 128 bits (322), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 85/344 (24%), Positives = 172/344 (50%), Gaps = 14/344 (4%) Query: 10 SGCWDTKDINKEYMPAVMGIGKGKTEKYRVILQVPNAT---GRTLF-LEKEAKSITKAVN 65 SGCWD++DIN MP +MG+ K + + + V +P + R L ++ + ++ A Sbjct: 20 SGCWDSRDINYRSMPIMMGVTK-EEDLFHVYFIIPVSVVSEQRNLAKVDGKGATVLDATE 78 Query: 66 LIQTDAEKDISLVHLRLLLIDKELAEEGISNIVDFAVRANDISIKGMMAVVDGDFEKTLY 125 I+ E I L H+ ++ID++L EEGI I++ +R D+ +A +D D K + Sbjct: 79 QIRKKIETTIDLEHIDFVVIDEKLGEEGIEKIINTFIRMRDMPASPAVAFIDEDMNKYME 138 Query: 126 HEISPTPEVSS--YDFFSEEAGWTPNQSIIRIWEAYRSLNSYSEDMAIPMLKNGEHTLFV 183 IS EV + + F ++ GW + +WE YR +NSY+ D+A+P++ + + Sbjct: 139 MMISNFEEVGTPIFGFLEKQVGWDIYDPEVSLWELYRGINSYTTDIAVPLIGSNKDLTMD 198 Query: 184 FKGTAVMKEDRMIGRLDQEETLLYNLFKGKYTGGT-IELARNTSVLVENAKIKHHREWSK 242 + G +V+K +++ + E ++ NL + T + + T+V + + + + Sbjct: 199 YLGASVLKNGKIVDTISSVEVMIMNLLDNEEVIKTFVTIDDTTNVELVDRDVNYDYNKRG 258 Query: 243 EGPSLTSDITLNVVIAESPKGVSNDQIEKEVWKQLDRKFNETLKKITGYHSDVLGVGLLY 302 E LT + +++AE+ +++ I++ ++++R+ + ++K+ D+LG+ Sbjct: 259 EEIDLTIKLEFVLIVAEAEDDLADKDIKQMFEEKIERETLQLVQKLQEQQVDILGISNRL 318 Query: 303 RPLLSETEITAWKSRWFPALKQQINVKVNVLNEIYFKNTDQDRN 346 R + E+ + + +P N+ VNV E +N + RN Sbjct: 319 RTRYTNAELKNFVNEVYP------NINVNVEVEAVIENNGKLRN 356 >B1DHB8.1 B1DHB8_9BACL Putative uncharacterized protein Length = 252 Score = 89.0 bits (219), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 51/186 (27%), Positives = 92/186 (49%), Gaps = 11/186 (5%) Query: 8 LLSGCWDTKDINKEYMPAVMGIGKGKTEKYRVILQVPNATGRT-------LFLEKEAKSI 60 ++SGCWD+KD++ M V+G+ K V + P G L + +++ Sbjct: 20 IVSGCWDSKDVDNRMMVGVIGLEKSTDSSLNVWFRFPLPKGTQENKKDDFFTLSQYGETV 79 Query: 61 TKAVNLIQTDAEKDISLVHLRLLLIDKELAEEGISNIVDFAVRANDISIKGMMAVVDGDF 120 + A++ ++ K + R +L+D+ LA+EG+ ++FA+R + + ++AV+ GD Sbjct: 80 SDAMSKVKYKLPKALDPSSTRAILLDESLAKEGLDPYLEFAIRERSVPLDAVVAVIRGDM 139 Query: 121 EKTLYHEISPTPEVSSYD---FFSEEAGWTPNQSIIRIWEAYRSLNSYSEDMAIPMLKNG 177 E+ ++ +PT E+S FF AG P ++ +WE Y L S IP+L G Sbjct: 140 ER-IFTNTNPTGELSGIYTKLFFETYAGGIPRKNKTMLWEVYSKLYSPFHTNLIPLLTEG 198 Query: 178 EHTLFV 183 + F Sbjct: 199 KQNXFC 204 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,070,939,098 Number of extensions: 85496073 Number of successful extensions: 221343 Number of sequences better than 1.0e-05: 156 Number of HSP's gapped: 221412 Number of HSP's successfully gapped: 156 Length of query: 354 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 217 Effective length of database: 1,770,017,148 Effective search space: 384093721116 Effective search space used: 384093721116 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)