BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0033.p (281 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGI5.1 B3KGI5_9BACI PTS system, glucose subfamily, IIA subunit 265 4e-69 B1DHR6.1 B1DHR6_9BACL PTS system, glucose subfamily, IIA subunit 176 2e-42 Q0BBN6.1 Q0BBN6_BURCM PTS system, N-acetylglucosamine-specific I... 126 2e-27 B9CBV1.1 B9CBV1_9BURK Pts system, N-acetylglucosamine-specific i... 126 2e-27 B9BU54.1 B9BU54_9BURK Pts system, N-acetylglucosamine-specific i... 126 2e-27 >B3KGI5.1 B3KGI5_9BACI PTS system, glucose subfamily, IIA subunit Length = 627 Score = 265 bits (677), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 139/231 (60%), Positives = 167/231 (72%), Gaps = 1/231 (0%) Query: 1 LGRAIMLPTMVLPAAAILLSLGSLPWSAWGLSSVSEVTTYAGQGIFYFMPYLFAVGVAWG 60 LGRA+MLPTM LPAAA+LLS+GSLPWSAWG ++ E+ AG G+FY+MPYLFA+GVAWG Sbjct: 10 LGRAVMLPTMALPAAALLLSVGSLPWSAWGFGTLGEIAQAAGHGVFYYMPYLFAIGVAWG 69 Query: 61 LSNQXXXXXXXXXXXXFTYDRIVSNMGDGAVQPATXXXXXXXXXXXXXHNRFKNIKLPEA 120 LSNQ F YD+++S +GDG+VQP+T +NRFK+IKLPEA Sbjct: 70 LSNQGGPAGLAALAGMFIYDQVISRLGDGSVQPSTLIGIIFGVMSGYVYNRFKHIKLPEA 129 Query: 121 IQFFGGSRFVLLFMGLFSALFAWVMLGLSPVLQHGLDILFRDIQATXXXXXXXXXXXXXX 180 IQFFGGSRFVLLFMGLFS +FAW+MLG +P++Q GLD + Sbjct: 130 IQFFGGSRFVLLFMGLFSTIFAWIMLGTAPLVQRGLDAFYELTVHMGAFGLFLYGILYRV 189 Query: 181 LTAFGLHHILNNVFWFQLGTFTTPDGSAVVQGDLPRFFAGDPTAGIFMAGL 231 LTAFGLHH+LNNV+WFQLG++ T DG+ VVQGDLPRFFAGDPTAG FMAGL Sbjct: 190 LTAFGLHHLLNNVYWFQLGSYETEDGT-VVQGDLPRFFAGDPTAGDFMAGL 239 >B1DHR6.1 B1DHR6_9BACL PTS system, glucose subfamily, IIA subunit Length = 628 Score = 176 bits (447), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 97/231 (41%), Positives = 133/231 (57%), Gaps = 2/231 (0%) Query: 1 LGRAIMLPTMVLPAAAILLSLGSLPWSAWGLSSVSEVTTYAGQGIFYFMPYLFAVGVAWG 60 LGRA MLP +VLPAAAI L L LPW G+ + ++ AG IF F+PY+FA+GV+ G Sbjct: 10 LGRAFMLPMIVLPAAAIFLFLSQLPWDLLGIPNAADYLQAAGHAIFTFLPYIFALGVSLG 69 Query: 61 LSNQXXXXXXXXXXXXFTYDRIVSNMGDGAVQPATXXXXXXXXXXXXXHNRFKNIKLPEA 120 L+ Y V+ D ++P + RFKN++LPE Sbjct: 70 LTGNALAAGMAALAGMVIYVG-VTGTADPQIEPTVFVGSIIGMISGFSYERFKNLRLPEY 128 Query: 121 IQFFGGSRFVLLFMGLFSALFAWVMLGLSPVLQHGLDILFRDIQATXXXXXXXXXXXXXX 180 IQFFGG RFV LF+ + + + + M+G++P++Q L+ L + + Sbjct: 129 IQFFGGPRFVPLFVSVVTVILSMAMIGVAPLIQRMLEGLGDIVASGGGFGVFLFGFLYRI 188 Query: 181 LTAFGLHHILNNVFWFQLGTFTTPDGSAVVQGDLPRFFAGDPTAGIFMAGL 231 L FGLHH+L++VFWFQ+G + T DGS +V GDLPRFFAGDPTAG FMAG+ Sbjct: 189 LVVFGLHHLLSHVFWFQVGGYETADGS-MVYGDLPRFFAGDPTAGAFMAGM 238 >Q0BBN6.1 Q0BBN6_BURCM PTS system, N-acetylglucosamine-specific IIBC subunit Length = 585 Score = 126 bits (317), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 81/230 (35%), Positives = 110/230 (47%), Gaps = 6/230 (2%) Query: 1 LGRAIMLPTMVLPAAAILLSLGSLPWSAWGLSSVSEVTTYAGQGIFYFMPYLFAVGVAWG 60 LGRA+MLP VLP A ILL LG + + +++ AG IF +P LFA+GVA G Sbjct: 12 LGRALMLPIAVLPVAGILLRLGQ--PDVFNIKMIAD----AGGAIFDNLPLLFAIGVAVG 65 Query: 61 LSNQXXXXXXXXXXXXFTYDRIVSNMGDGAVQPATXXXXXXXXXXXXXHNRFKNIKLPEA 120 + + + + D + +NR+K+IKLP+ Sbjct: 66 FAKDNNGVAALAGAIGYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDY 125 Query: 121 IQFFGGSRFVLLFMGLFSALFAWVMLGLSPVLQHGLDILFRDIQATXXXXXXXXXXXXXX 180 + FFGG RFV + GL + V + +QH +D + + Sbjct: 126 LAFFGGKRFVPIITGLVCVILGIVFGYVWQPVQHAIDAAGQWLTTAGAIGAFVFGFLNRL 185 Query: 181 LTAFGLHHILNNVFWFQLGTFTTPDGSAVVQGDLPRFFAGDPTAGIFMAG 230 L GLHHI+N++ WF G FT P G +V GDL RFFAGDPTAG FMAG Sbjct: 186 LLVTGLHHIINSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAG 235 >B9CBV1.1 B9CBV1_9BURK Pts system, N-acetylglucosamine-specific iibc component EC=2.7.1.69 Length = 596 Score = 126 bits (316), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 81/230 (35%), Positives = 110/230 (47%), Gaps = 6/230 (2%) Query: 1 LGRAIMLPTMVLPAAAILLSLGSLPWSAWGLSSVSEVTTYAGQGIFYFMPYLFAVGVAWG 60 LGRA+MLP VLP A ILL LG + + +++ AG IF +P LFA+GVA G Sbjct: 12 LGRALMLPIAVLPVAGILLRLGQ--PDVFNIKMIAD----AGGAIFDNLPLLFAIGVAVG 65 Query: 61 LSNQXXXXXXXXXXXXFTYDRIVSNMGDGAVQPATXXXXXXXXXXXXXHNRFKNIKLPEA 120 + + + + D + +NR+K+IKLP+ Sbjct: 66 FAKDNNGVAALAGAIGYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDY 125 Query: 121 IQFFGGSRFVLLFMGLFSALFAWVMLGLSPVLQHGLDILFRDIQATXXXXXXXXXXXXXX 180 + FFGG RFV + GL + V + +QH +D + + Sbjct: 126 LAFFGGKRFVPIITGLVCVVLGIVFGYVWQPIQHAIDAAGQWLTTAGAIGAFVFGFLNRL 185 Query: 181 LTAFGLHHILNNVFWFQLGTFTTPDGSAVVQGDLPRFFAGDPTAGIFMAG 230 L GLHHI+N++ WF G FT P G +V GDL RFFAGDPTAG FMAG Sbjct: 186 LLVTGLHHIINSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAG 235 >B9BU54.1 B9BU54_9BURK Pts system, N-acetylglucosamine-specific iibc component EC=2.7.1.69 Length = 596 Score = 126 bits (316), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 81/230 (35%), Positives = 110/230 (47%), Gaps = 6/230 (2%) Query: 1 LGRAIMLPTMVLPAAAILLSLGSLPWSAWGLSSVSEVTTYAGQGIFYFMPYLFAVGVAWG 60 LGRA+MLP VLP A ILL LG + + +++ AG IF +P LFA+GVA G Sbjct: 12 LGRALMLPIAVLPVAGILLRLGQ--PDVFNIKMIAD----AGGAIFDNLPLLFAIGVAVG 65 Query: 61 LSNQXXXXXXXXXXXXFTYDRIVSNMGDGAVQPATXXXXXXXXXXXXXHNRFKNIKLPEA 120 + + + + D + +NR+K+IKLP+ Sbjct: 66 FAKDNNGVAALAGAIGYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDY 125 Query: 121 IQFFGGSRFVLLFMGLFSALFAWVMLGLSPVLQHGLDILFRDIQATXXXXXXXXXXXXXX 180 + FFGG RFV + GL + V + +QH +D + + Sbjct: 126 LAFFGGKRFVPIITGLVCVVLGIVFGYVWQPIQHAIDAAGQWLTTAGAIGAFVFGFLNRL 185 Query: 181 LTAFGLHHILNNVFWFQLGTFTTPDGSAVVQGDLPRFFAGDPTAGIFMAG 230 L GLHHI+N++ WF G FT P G +V GDL RFFAGDPTAG FMAG Sbjct: 186 LLVTGLHHIINSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAG 235 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.143 0.456 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,550,477,459 Number of extensions: 58730164 Number of successful extensions: 152123 Number of sequences better than 1.0e-05: 893 Number of HSP's gapped: 151122 Number of HSP's successfully gapped: 1004 Length of query: 281 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 147 Effective length of database: 1,798,280,193 Effective search space: 264347188371 Effective search space used: 264347188371 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)