BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0032.p (346 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGI5.1 B3KGI5_9BACI PTS system, glucose subfamily, IIA subunit 560 e-158 B1DHR6.1 B1DHR6_9BACL PTS system, glucose subfamily, IIA subunit 468 e-130 Q65KB7.1 Q65KB7_BACLD PtsG 259 2e-67 B7GIF8.1 B7GIF8_ANOFW Phosphotransferase system (PTS) glucose-sp... 258 9e-67 Q5L1A4.1 Q5L1A4_GEOKA PTS system, glucose-specific enzyme II, A ... 257 9e-67 >B3KGI5.1 B3KGI5_9BACI PTS system, glucose subfamily, IIA subunit Length = 627 Score = 560 bits (1444), Expect = e-158, Method: Compositional matrix adjust. Identities = 272/346 (78%), Positives = 306/346 (88%) Query: 1 VSEQIEFAFLFASPYLFGLHVVMSGLAMVLTYALGIHHGFSYSAGAIDFFLNMHLSQRAW 60 VSEQIEFAFLFASPYL+ LH +M+G AMVLTYA IHHGFSYSAG ID+ LN HLSQ AW Sbjct: 282 VSEQIEFAFLFASPYLYVLHALMAGFAMVLTYAFDIHHGFSYSAGFIDYVLNFHLSQNAW 341 Query: 61 LLIPXXXXXXXXXXNVFRWAIRRFQIPTPGREEGSELGDWAGNIPYQAPLILEALGGKEN 120 ++IP +FRWAIR F+IPTPGREEGS L AG+IPYQAPLILEALGGK+N Sbjct: 342 IIIPIGAVYGIVYYFLFRWAIRTFEIPTPGREEGSALEGRAGDIPYQAPLILEALGGKDN 401 Query: 121 IVQVQACITRLRLTVHNDRYIDTVALKGLGSAGIIKLGGGNVQVVFGTYSELIREEINKL 180 IVQVQ+C+TRLRLTV+NDR ID+ ALK LGSAGIIKLGGGNVQVVFGTYSELIREEINKL Sbjct: 402 IVQVQSCMTRLRLTVYNDRQIDSDALKLLGSAGIIKLGGGNVQVVFGTYSELIREEINKL 461 Query: 181 LLRDLPQVLFSSPIQGRMMPIEEVPDHIFAAKLVGDGVAFFPEKGELVSPVFGKVMHVYP 240 + +DLP+VLFS+P+QGRM+PI+EVPD IFAAKLVG+GVAF PEKGELVSPV+G VMHVYP Sbjct: 462 MQQDLPRVLFSAPVQGRMLPIDEVPDDIFAAKLVGNGVAFIPEKGELVSPVYGTVMHVYP 521 Query: 241 TMHAVGISTPEGLEVLMHIGIDTSQLKGPFEALVQEGDSVEPGQLLVRFDLTYLRDHAPS 300 TMHA+GISTPEGLEVL+HIGIDTSQLKGPF A+V EGD+VEPGQLLV+FDL YL+ HA S Sbjct: 522 TMHAIGISTPEGLEVLIHIGIDTSQLKGPFTAVVSEGDTVEPGQLLVKFDLAYLKTHAAS 581 Query: 301 LATPMVITNPDRVKSWSYAPFKNVKKGQSSVMSVVLHESNVGGVEA 346 LATPMVITNPD+V+SWSYAPFK VK+GQSSVMSVVL+ESN GG E+ Sbjct: 582 LATPMVITNPDKVRSWSYAPFKTVKRGQSSVMSVVLNESNAGGRES 627 >B1DHR6.1 B1DHR6_9BACL PTS system, glucose subfamily, IIA subunit Length = 628 Score = 468 bits (1203), Expect = e-130, Method: Compositional matrix adjust. Identities = 229/345 (66%), Positives = 276/345 (80%), Gaps = 2/345 (0%) Query: 1 VSEQIEFAFLFASPYLFGLHVVMSGLAMVLTYALGIHHGFSYSAGAIDFFLNMHLSQRAW 60 V+E +EFAFLF +PYLF +H ++SG+ M +TY LGI HGFS+S G ID+ +N HL+ R W Sbjct: 281 VTEPVEFAFLFVAPYLFVVHAILSGVIMWVTYELGIRHGFSFSGGVIDYIINEHLATRGW 340 Query: 61 LLIPXXXXXXXXXXNVFRWAIRRFQIPTPGREEGSELGDWAGNIPYQAPLILEALGGKEN 120 LLIP VFRWAI RF+IPTPGREEGS+L +WAG+IPY+APLIL+A+GGK+N Sbjct: 341 LLIPIGIVVGLVYYLVFRWAINRFRIPTPGREEGSQLDEWAGDIPYRAPLILQAIGGKDN 400 Query: 121 IVQVQACITRLRLTVHNDRYIDTVALKGLGSAGIIKLGGGNVQVVFGTYSELIREEINKL 180 IVQ+++CITRLRL V N++ +D AL+ LG+AG+++LGGGNVQVVFGTYSELIR E+ K Sbjct: 401 IVQMESCITRLRLKVSNEKLLDNNALRNLGAAGVLRLGGGNVQVVFGTYSELIRAEMLKA 460 Query: 181 LLRDLPQVLFSSPIQGRMMPIEEVPDHIFAAKLVGDGVAFFPEKGELVSPVFGKVMHVYP 240 + RD QVLFSSP+QGRM+P+E+VPD IFA KLVG GVAF PEKGELVSPV G VM VYP Sbjct: 461 IQRDQEQVLFSSPVQGRMIPLEDVPDPIFAGKLVGQGVAFIPEKGELVSPVKGTVMIVYP 520 Query: 241 TMHAVGISTPEGLEVLMHIGIDTSQLKGP--FEALVQEGDSVEPGQLLVRFDLTYLRDHA 298 TMHAVGI T EGLEVL+HIGIDTS+L G F+A+V+EG V PG +LVRFDL +R + Sbjct: 521 TMHAVGIRTQEGLEVLLHIGIDTSKLTGKSYFKAVVEEGQEVMPGDMLVRFDLQRVRKES 580 Query: 299 PSLATPMVITNPDRVKSWSYAPFKNVKKGQSSVMSVVLHESNVGG 343 SLATPMVITN D V SW +APFK VKKGQSSV+SVVL E N GG Sbjct: 581 KSLATPMVITNSDLVHSWRFAPFKAVKKGQSSVLSVVLKERNGGG 625 >Q65KB7.1 Q65KB7_BACLD PtsG Length = 698 Score = 259 bits (663), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 142/371 (38%), Positives = 217/371 (58%), Gaps = 41/371 (11%) Query: 1 VSEQIEFAFLFASPYLFGLHVVMSGLAMVLTYALGIHHGFSYSAGAIDFFLNMHLSQRA- 59 ++E IEF+FLF +P LF +H V +GL+ ++ L + G ++S G ID+FL L R Sbjct: 330 ITEPIEFSFLFVAPILFAIHCVFAGLSFMMMDILNVKIGMTFSGGLIDYFLFGILPNRTA 389 Query: 60 -WLLIPXXXXXXXXXXNVFRWAIRRFQIPTPGREEGS-----ELGDWAGNIPYQAPLILE 113 WL+IP FR+AIR+F + TPGRE+ + E + AG++PYQ ILE Sbjct: 390 WWLVIPVGLVLAVIYYFGFRFAIRKFNLKTPGREDAAAEPADEGKEAAGDLPYQ---ILE 446 Query: 114 ALGGKENIVQVQACITRLRLTVHNDRYIDTVALKGLGSAGIIKLGGGNVQVVFGTYSELI 173 A+G +ENI + ACITRLR+TV++ + +D LK LG++G++++G N+Q +FG S+ + Sbjct: 447 AMGDQENIKHLDACITRLRVTVNDQKKVDKERLKKLGASGVLEVGN-NIQAIFGPRSDNL 505 Query: 174 REEINKLLLRDLP-----------------------------QVLFSSPIQGRMMPIEEV 204 + ++ ++ P + +F SP+ G + PI +V Sbjct: 506 KSQMQDIMSGRTPRPEKAPNAAEEVEQQIEEAMVEPLQNESGEEVFVSPLAGELHPIADV 565 Query: 205 PDHIFAAKLVGDGVAFFPEKGELVSPVFGKVMHVYPTMHAVGISTPEGLEVLMHIGIDTS 264 PD +F+ K++GDG A P +G +VSPV GKV++V+PT HA+G+ + G E+L+H GIDT Sbjct: 566 PDQVFSGKMMGDGFAILPAEGTVVSPVRGKVLNVFPTKHAIGLQSDGGREILIHFGIDTV 625 Query: 265 QLKGP-FEALVQEGDSVEPGQLLVRFDLTYLRDHAPSLATPMVITNPDRVKSWSYAPFKN 323 LKG F + V EGD VEPGQ L+ D+ ++D+ PSL TP+V TN +S S N Sbjct: 626 SLKGEGFTSFVSEGDRVEPGQKLLEVDIEKVKDNVPSLMTPIVFTNLQEGESVSLQAEGN 685 Query: 324 VKKGQSSVMSV 334 V Q ++ + Sbjct: 686 VSLTQDDIVRI 696 >B7GIF8.1 B7GIF8_ANOFW Phosphotransferase system (PTS) glucose-specific enzyme IICBA component Length = 663 Score = 258 bits (658), Expect = 9e-67, Method: Compositional matrix adjust. Identities = 138/354 (38%), Positives = 219/354 (61%), Gaps = 26/354 (7%) Query: 1 VSEQIEFAFLFASPYLFGLHVVMSGLAMVLTYALGIHHGFSYSAGAIDFFLNMHLSQRA- 59 ++E IEF+FLF +P LF +H + +GL+ +L + L + G ++S G ID+ L L R Sbjct: 311 ITEPIEFSFLFVAPALFAIHTIFAGLSFMLMHILNVKIGMTFSGGVIDYLLFGVLPGRTA 370 Query: 60 -WLLIPXXXXXXXXXXNVFRWAIRRFQIPTPGREEGSELGDW---AGNIPYQAPLILEAL 115 WL+IP FR+AIR++ + TPGRE+ + AG++P++ IL AL Sbjct: 371 WWLVIPVGLVFAVIYYFGFRFAIRKWDLATPGREKETTETTTTADAGDLPHE---ILAAL 427 Query: 116 GGKENIVQVQACITRLRLTVHNDRYIDTVALKGLGSAGIIKLGGGNVQVVFGTYSELIRE 175 GGKENI + ACITRLR++V++ +++D LK LG+AG++++G N+Q +FG S++++ Sbjct: 428 GGKENIAHLDACITRLRVSVNDIQHVDKDRLKALGAAGVLEVGN-NIQAIFGPKSDILKT 486 Query: 176 EINKLLLRDLPQVLF----------------SSPIQGRMMPIEEVPDHIFAAKLVGDGVA 219 ++ ++ +P +SP+ G+++P+ EVPD +F+ K++GDG A Sbjct: 487 QMKDIMEGRVPTKAVEKAKPVVQATGETEQVASPLTGKVVPLSEVPDQVFSTKMMGDGFA 546 Query: 220 FFPEKGELVSPVFGKVMHVYPTMHAVGISTPEGLEVLMHIGIDTSQLKGP-FEALVQEGD 278 P G +VSPV G +++V+PT HA+GI + G EVL+H GIDT +L G FEALV EG Sbjct: 547 VEPTDGTVVSPVDGTIVNVFPTKHAIGIQSKTGREVLIHFGIDTVKLNGQGFEALVSEGT 606 Query: 279 SVEPGQLLVRFDLTYLRDHAPSLATPMVITNPDRVKSWSYAPFKNVKKGQSSVM 332 V+ GQ ++ DL Y++ HAPS+ TP++ TN ++ + A NV KG+ V+ Sbjct: 607 EVKKGQPILNVDLAYVKQHAPSIVTPIIFTNLQAGETVNIAKQGNVTKGEDVVV 660 >Q5L1A4.1 Q5L1A4_GEOKA PTS system, glucose-specific enzyme II, A component Length = 672 Score = 257 bits (657), Expect = 9e-67, Method: Compositional matrix adjust. Identities = 140/358 (39%), Positives = 219/358 (61%), Gaps = 28/358 (7%) Query: 1 VSEQIEFAFLFASPYLFGLHVVMSGLAMVLTYALGIHHGFSYSAGAIDFFLNMHLSQRA- 59 ++E IEF+FLF +P LF +H V +GL+ ++ + L + G ++S G IDF L L R Sbjct: 318 ITEPIEFSFLFVAPALFAIHCVFAGLSFMIMHLLNVKIGMTFSGGVIDFLLFGVLPNRTA 377 Query: 60 -WLLIPXXXXXXXXXXNVFRWAIRRFQIPTPGREE-----GSELGDWAGNIPYQAPLILE 113 WL+IP FR+AIR++ + TPGRE+ AG++PY+ +L Sbjct: 378 WWLVIPVGLVFAVIYYFGFRFAIRKWDLATPGREKTVEEAPKAEAAAAGDLPYE---VLA 434 Query: 114 ALGGKENIVQVQACITRLRLTVHNDRYIDTVALKGLGSAGIIKLGGGNVQVVFGTYSELI 173 ALGGKENI + ACITRLR++VH+ +D LK LG+AG++++G NVQ +FG S+++ Sbjct: 435 ALGGKENIEHLDACITRLRVSVHDIGQVDKDRLKALGAAGVLEVGN-NVQAIFGPKSDML 493 Query: 174 REEINKLLLRDLP----------------QVLFSSPIQGRMMPIEEVPDHIFAAKLVGDG 217 + +I ++ P +SP+ G ++P+ EVPD +F+ K++GDG Sbjct: 494 KGQIQDIMQGKAPARAEEKPKAAAPEAAKSETIASPMSGEVVPLAEVPDQVFSQKMMGDG 553 Query: 218 VAFFPEKGELVSPVFGKVMHVYPTMHAVGISTPEGLEVLMHIGIDTSQLKGP-FEALVQE 276 A P G +VSPV GK+++V+PT HA+GI + G E+L+H+GIDT +L G FEALV+E Sbjct: 554 FAVMPTDGTVVSPVDGKIINVFPTKHAIGIQSAGGHEMLIHVGIDTVKLNGQGFEALVKE 613 Query: 277 GDSVEPGQLLVRFDLTYLRDHAPSLATPMVITNPDRVKSWSYAPFKNVKKGQSSVMSV 334 GD V+ GQ ++R DL Y++ +APS+ TP++ TN ++ V +G+ +V+++ Sbjct: 614 GDEVKKGQPILRVDLDYVKQNAPSIVTPVIFTNLKAGETVHVNKQGPVARGEDAVVTI 671 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,026,506,538 Number of extensions: 83765535 Number of successful extensions: 189264 Number of sequences better than 1.0e-05: 3188 Number of HSP's gapped: 183972 Number of HSP's successfully gapped: 3236 Length of query: 346 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 210 Effective length of database: 1,779,438,163 Effective search space: 373682014230 Effective search space used: 373682014230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)