BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0031.p (588 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGI6.1 B3KGI6_9BACI Phosphoenolpyruvate-protein phosphotransfe... 867 0.0 B1DHR5.1 B1DHR5_9BACL Phosphoenolpyruvate-protein phosphotransfe... 759 0.0 A6TMP7.1 A6TMP7_ALKMQ Phosphoenolpyruvate-protein phosphotransfe... 519 e-145 B7DSU9.1 B7DSU9_9BACL Phosphoenolpyruvate-protein phosphotransfe... 513 e-143 B9MM71.1 B9MM71_ANATD Phosphoenolpyruvate-protein phosphotransfe... 511 e-143 >B3KGI6.1 B3KGI6_9BACI Phosphoenolpyruvate-protein phosphotransferase Length = 587 Score = 867 bits (2240), Expect = 0.0, Method: Compositional matrix adjust. Identities = 421/573 (73%), Positives = 492/573 (85%) Query: 1 MIQXXXXXXXXXXXKAFVLPNWEWSLPDTQVNPVDLAKEFERLYEGIRTSKDEIEFIKRE 60 MIQ KAFVLP+WE LPD++++ VDLAKEFERLYEGIRTSK+EIE+IK E Sbjct: 1 MIQGIGAATGVAIGKAFVLPHWELDLPDSEMDAVDLAKEFERLYEGIRTSKNEIEYIKNE 60 Query: 61 FREVVGPEESSIFDAHLAILDDPVFMSEIRGIIERQYKAAEVAVKEAIDHFVAMFDLLDD 120 F+E+VG EES IFDAHLAIL+DPVFM+EI+GIIERQYKAAEVAVKEAIDHFV+MFDLLDD Sbjct: 61 FKEMVGHEESQIFDAHLAILEDPVFMNEIQGIIERQYKAAEVAVKEAIDHFVSMFDLLDD 120 Query: 121 EYMKERAVDIKDVGNRLLKHLLGAPEVTLPSDTQPYILVAKELSPSQLAHLNPTYVLGIV 180 EYMKERA+DIKDVGNRLLKHLLGAPEV LP+DTQPYILVAKELSPSQL HLNP++VLGIV Sbjct: 121 EYMKERALDIKDVGNRLLKHLLGAPEVKLPADTQPYILVAKELSPSQLVHLNPSHVLGIV 180 Query: 181 TMMGGKTSHSSIMARALGIPLVAGLENKILTPIQTGDLLVMDGETGVIQLYPDELTLKEY 240 TM GGKTSHS+IMARALGIPLV+G+ENK+ PIQTG LV+DG+ G++ L PD L ++ Y Sbjct: 181 TMTGGKTSHSAIMARALGIPLVSGIENKLDKPIQTGQQLVVDGDEGLLYLEPDTLIVERY 240 Query: 241 TSRRAKQQRKKEQLELLATVDAVTKDGVQLRLAGNISSVKELDMALKYGAQGVGLFRTEF 300 R +Q++KKEQL+LLA VDAVTKDG LA N++SVKE+++ALK GAQGVGLFRTEF Sbjct: 241 EGIRDQQRKKKEQLQLLAAVDAVTKDGTYFNLAANMNSVKEMEIALKNGAQGVGLFRTEF 300 Query: 301 LYMDRSSFPTEEEQFEVYKLVAEKVGNETVVIRTLDIGGDKHLDYFQLPDEQNPFLGYRA 360 LYMDR +FPTEEEQFEVYKLVAE G +VVIRTLDIGGDK LDYF LP+E+NPFLGYRA Sbjct: 301 LYMDRHNFPTEEEQFEVYKLVAENAGQASVVIRTLDIGGDKQLDYFALPEEENPFLGYRA 360 Query: 361 IRISLDRKDMFKTQMTAILRASHYGNIKMMFPMISSVEEVQAAKAVLNEVKEELDQQGVP 420 IRISLDR + F+TQ+TAILRAS YG++K+M+PMISSVEEV+ A AVL +LD++G+ Sbjct: 361 IRISLDRTEPFQTQLTAILRASAYGSVKIMYPMISSVEEVKQANAVLRRAMADLDRRGIA 420 Query: 421 YNRNIPVGIMIEVPAAVMIADLLAEEVDFFSIGTNDLVQYVLAVDRMNEQIAHMYHPYHP 480 Y+ NI VGIMIEVPAAV I+DLLAEE DFFSIGTNDLVQYVLAVDRMNEQIAHMYHP+HP Sbjct: 421 YDPNIKVGIMIEVPAAVTISDLLAEEADFFSIGTNDLVQYVLAVDRMNEQIAHMYHPFHP 480 Query: 481 GVLRMIRMTVEAARRVGIDISVCGEMAADERSLPLWLEMGISDLSMSPQALLKVKHRALN 540 +LRM+R TVEAA GI +SVCGE+A DER++PLWLE+G+ DLSMSPQ+LL+VKHR LN Sbjct: 481 AILRMLRTTVEAAHGAGIPVSVCGELAGDERAIPLWLELGVKDLSMSPQSLLRVKHRILN 540 Query: 541 TLASEARETAKSCFRHRTSTETEEILTAFAGRS 573 T A+EA+ A +CF+ RTS+ EE L+AFA RS Sbjct: 541 TNAAEAKGEAAACFKLRTSSAVEERLSAFAERS 573 >B1DHR5.1 B1DHR5_9BACL Phosphoenolpyruvate-protein phosphotransferase Length = 582 Score = 759 bits (1959), Expect = 0.0, Method: Compositional matrix adjust. Identities = 363/569 (63%), Positives = 449/569 (78%) Query: 1 MIQXXXXXXXXXXXKAFVLPNWEWSLPDTQVNPVDLAKEFERLYEGIRTSKDEIEFIKRE 60 MIQ K+FVLPNWEW P+ +V+ D A+EFER+YEGIRTSK EIE IK E Sbjct: 1 MIQGIGASAGIAIGKSFVLPNWEWEFPEQRVDVADFAREFERVYEGIRTSKHEIEQIKDE 60 Query: 61 FREVVGPEESSIFDAHLAILDDPVFMSEIRGIIERQYKAAEVAVKEAIDHFVAMFDLLDD 120 REVVG +E+ IFDAHLAILDDPVFM+EI+GII+RQYKAAEVAVKEAIDHFV MFDLLDD Sbjct: 61 LREVVGSDETQIFDAHLAILDDPVFMNEIQGIIQRQYKAAEVAVKEAIDHFVTMFDLLDD 120 Query: 121 EYMKERAVDIKDVGNRLLKHLLGAPEVTLPSDTQPYILVAKELSPSQLAHLNPTYVLGIV 180 EYMKERA+DIKDVGNRLLKHLLGAPE+TLPSDTQP+IL+A+ELSPSQLAH+NP VLGIV Sbjct: 121 EYMKERALDIKDVGNRLLKHLLGAPEITLPSDTQPFILIARELSPSQLAHINPNNVLGIV 180 Query: 181 TMMGGKTSHSSIMARALGIPLVAGLENKILTPIQTGDLLVMDGETGVIQLYPDELTLKEY 240 T+ G TSHS+IMARALG+PLV G+E K+ PIQTG LV+DG G++ + PD+ ++ Y Sbjct: 181 TLAGSSTSHSAIMARALGVPLVVGVEAKLEEPIQTGQSLVIDGTEGLLFIEPDDSLIEHY 240 Query: 241 TSRRAKQQRKKEQLELLATVDAVTKDGVQLRLAGNISSVKELDMALKYGAQGVGLFRTEF 300 + + + +L +A +VT DG L LA NISS+KEL++AL GA GVGLFRTEF Sbjct: 241 SQLQTSLAEARHRLSGIAGHQSVTPDGKVLGLAANISSLKELEVALSSGAHGVGLFRTEF 300 Query: 301 LYMDRSSFPTEEEQFEVYKLVAEKVGNETVVIRTLDIGGDKHLDYFQLPDEQNPFLGYRA 360 LYMDR+ FP EEEQFEVYK VAEK+ E ++IRTLDIGGDK LDYF +P+E NPFLGYRA Sbjct: 301 LYMDRNRFPKEEEQFEVYKSVAEKLAGEPLIIRTLDIGGDKQLDYFNIPEEDNPFLGYRA 360 Query: 361 IRISLDRKDMFKTQMTAILRASHYGNIKMMFPMISSVEEVQAAKAVLNEVKEELDQQGVP 420 IRISLDRKD+FKTQ+ AILRAS YG +K+M+P+ISSVEEV+AA VL E K+EL+++G+ Sbjct: 361 IRISLDRKDLFKTQLRAILRASSYGQVKVMYPLISSVEEVRAANQVLLEAKQELEREGIA 420 Query: 421 YNRNIPVGIMIEVPAAVMIADLLAEEVDFFSIGTNDLVQYVLAVDRMNEQIAHMYHPYHP 480 Y+ I VGIM+EVPAAVM++DLLA+EVDFFSIGTNDLVQ+ LAVDRMN++I+++Y P+HP Sbjct: 421 YDPGIQVGIMVEVPAAVMLSDLLAQEVDFFSIGTNDLVQFTLAVDRMNDEISYLYEPFHP 480 Query: 481 GVLRMIRMTVEAARRVGIDISVCGEMAADERSLPLWLEMGISDLSMSPQALLKVKHRALN 540 VLRM++ TVEAA+R GI + VCGE+A D R+LP+WL + + ++SMS ++L+VK R L Sbjct: 481 AVLRMLKQTVEAAKRNGIKVGVCGELAGDLRALPIWLGLDVDEISMSSHSILQVKERLLC 540 Query: 541 TLASEARETAKSCFRHRTSTETEEILTAF 569 T ++R R S + + L F Sbjct: 541 TTQKDSRSLLPQLLECRMSADIHQKLKQF 569 >A6TMP7.1 A6TMP7_ALKMQ Phosphoenolpyruvate-protein phosphotransferase Length = 571 Score = 519 bits (1336), Expect = e-145, Method: Compositional matrix adjust. Identities = 251/553 (45%), Positives = 381/553 (68%), Gaps = 3/553 (0%) Query: 15 KAFVLPNWEWSLPDTQVNPVDLAKEFERLYEGIRTSKDEIEFIKREFREVVGPEESSIFD 74 +A V E ++ V L KE ++ I+ SK ++E++K+E + +G +++SIF+ Sbjct: 15 RALVYQEQELNIKKESVE--GLEKELDKFQSSIKKSKVQLEYVKQEAMKKLGADKASIFE 72 Query: 75 AHLAILDDPVFMSEIRGIIERQYKAAEVAVKEAIDHFVAMFDLLDDEYMKERAVDIKDVG 134 AH+ +LDDP +++++ I + +AE AV E I+ F+ +F+ +D+EYMKERA DI+DVG Sbjct: 73 AHIMVLDDPELINQVKDKITNEKVSAEYAVNEVINSFITLFEGMDNEYMKERAADIRDVG 132 Query: 135 NRLLKHLLGAPEVTLPSDTQPYILVAKELSPSQLAHLNPTYVLGIVTMMGGKTSHSSIMA 194 NR+LK+LL + L + T ILVA +L+PS A +N VLG +T +GG+TSH++IMA Sbjct: 133 NRILKNLLNVEIINLATLTDEVILVANDLTPSDTATMNKEKVLGFITNIGGRTSHTAIMA 192 Query: 195 RALGIPLVAGLENKILTPIQTGDLLVMDGETGVIQLYPDELTLKEYTSRRAKQQRKKEQL 254 ++L +P + GL++ I +++GDLL+ DG G + PDE L ++ ++ + + +++L Sbjct: 193 KSLEVPAIVGLKS-ITDEVKSGDLLIFDGGEGFGHINPDEQLLADFREKKQQYEFIRKEL 251 Query: 255 ELLATVDAVTKDGVQLRLAGNISSVKELDMALKYGAQGVGLFRTEFLYMDRSSFPTEEEQ 314 E L + +V+KDG ++ L NI + K++ L G +G+GL+RTEFLYM+R P+EEEQ Sbjct: 252 EKLKSEPSVSKDGRRVELGANIGTPKDVKGVLDNGGEGIGLYRTEFLYMNRDHMPSEEEQ 311 Query: 315 FEVYKLVAEKVGNETVVIRTLDIGGDKHLDYFQLPDEQNPFLGYRAIRISLDRKDMFKTQ 374 +E YK V +K+ + V+IRTLDIGGDK L Y LP E NPFLGYRAIRI LD+ ++FK Q Sbjct: 312 YEAYKEVLQKMEEKPVIIRTLDIGGDKELPYLNLPKELNPFLGYRAIRICLDQPEIFKVQ 371 Query: 375 MTAILRASHYGNIKMMFPMISSVEEVQAAKAVLNEVKEELDQQGVPYNRNIPVGIMIEVP 434 + A+LRAS YGN+K+M+PMISSVEEV+AA +L EV+ ELDQ+G+ Y++ I VGIM+E+P Sbjct: 372 LRALLRASVYGNLKIMYPMISSVEEVRAANEILQEVRGELDQKGIEYSKEIEVGIMVEIP 431 Query: 435 AAVMIADLLAEEVDFFSIGTNDLVQYVLAVDRMNEQIAHMYHPYHPGVLRMIRMTVEAAR 494 A V+I D+L +EVDFFSIGTNDL+QY AVDRMNE+I+H+Y P+HP +LRMI+M ++ A Sbjct: 432 ATVVIGDMLIKEVDFFSIGTNDLIQYTTAVDRMNEKISHLYEPFHPAILRMIKMVIDHAH 491 Query: 495 RVGIDISVCGEMAADERSLPLWLEMGISDLSMSPQALLKVKHRALNTLASEARETAKSCF 554 G +CGEMA D + +P+ L +G+ + SMSP ++L+ + SE ++ A+ Sbjct: 492 GGGKWAGMCGEMAGDPKIVPILLGLGLDEFSMSPISILQARKIIRGLSYSEMKKIAEKAL 551 Query: 555 RHRTSTETEEILT 567 T+ E ++ + Sbjct: 552 NFPTAAEVKKFVA 564 >B7DSU9.1 B7DSU9_9BACL Phosphoenolpyruvate-protein phosphotransferase EC=2.7.3.9 Length = 568 Score = 513 bits (1320), Expect = e-143, Method: Compositional matrix adjust. Identities = 252/548 (45%), Positives = 365/548 (66%), Gaps = 3/548 (0%) Query: 15 KAFVLPNWEWSLPDTQVNPVDLAKEFERLYEGIRTSKDEIEFIKREFREVVGPEESSIFD 74 KA+VL E S+ +V D E+ R+ I ++K+E+E +++ + +G E+ +F Sbjct: 18 KAWVLAPSEPSVERVEV--ADPQAEWARVERAIASAKEELEALRQTALDKLGQAEAQLFV 75 Query: 75 AHLAILDDPVFMSEIRGIIERQYKAAEVAVKEAIDHFVAMFDLLDDEYMKERAVDIKDVG 134 AH +LDDP +IR IE + AE AV+ ID V +FD LDDEYM++RA D++DVG Sbjct: 76 AHAQMLDDPELTGQIRQAIEAERVNAEWAVQSVIDTLVGLFDALDDEYMRQRAADVRDVG 135 Query: 135 NRLLKHLLGAPEVTLPSDTQPYILVAKELSPSQLAHLNPTYVLGIVTMMGGKTSHSSIMA 194 +RLL+HL G +L P +LVA++L+PS L+P V VT +GG+TSH++IMA Sbjct: 136 SRLLRHLQGGEGASLADMVGPVVLVARDLTPSDTVQLDPALVRAFVTDIGGRTSHTAIMA 195 Query: 195 RALGIPLVAGLENKILTPIQTGDLLVMDGETGVIQLYPDELTLKEYTSRRAKQQRKKEQL 254 R+LGIP V GL I +Q G +L +DG G++ + PDE L ++++ +QQ + +L Sbjct: 196 RSLGIPAVVGL-GDITANVQAGQVLAVDGSEGLVVVEPDEAELARFSAQVERQQGARSRL 254 Query: 255 ELLATVDAVTKDGVQLRLAGNISSVKELDMALKYGAQGVGLFRTEFLYMDRSSFPTEEEQ 314 L +++T DG ++ +AGNI + E+ L G +G+GLFR+EFLYM+R + PTEEEQ Sbjct: 255 AALRDAESITPDGRRVEIAGNIGTPAEVAQVLAQGGEGIGLFRSEFLYMNRDAAPTEEEQ 314 Query: 315 FEVYKLVAEKVGNETVVIRTLDIGGDKHLDYFQLPDEQNPFLGYRAIRISLDRKDMFKTQ 374 F YK VAE + V++RTLD+GGDK + Y LP E NPFLGYRAIR+ LD+K++F Q Sbjct: 315 FAAYKEVAEAMAGRPVIVRTLDVGGDKAIPYLGLPHEDNPFLGYRAIRVCLDQKELFAAQ 374 Query: 375 MTAILRASHYGNIKMMFPMISSVEEVQAAKAVLNEVKEELDQQGVPYNRNIPVGIMIEVP 434 + AILRASHYG +++MFPMI++VEEV+AAK L K+ L ++GVP++ +I VGIMIE+P Sbjct: 375 LRAILRASHYGKLRVMFPMIATVEEVRAAKRELEAAKQALREEGVPFDEDIEVGIMIEIP 434 Query: 435 AAVMIADLLAEEVDFFSIGTNDLVQYVLAVDRMNEQIAHMYHPYHPGVLRMIRMTVEAAR 494 AA ++AD LA+EVDFFSIGTNDLVQY LA DR+NE+IAH+Y P +P VLR+++M ++ A Sbjct: 435 AAAVMADRLAKEVDFFSIGTNDLVQYTLACDRLNERIAHLYQPLNPSVLRLVKMVIDGAH 494 Query: 495 RVGIDISVCGEMAADERSLPLWLEMGISDLSMSPQALLKVKHRALNTLASEARETAKSCF 554 G + +CGE+A D R+ + L +G+ + SMS ++LKV+ NT +A+ A Sbjct: 495 AQGKWVGMCGELAGDPRATAILLGLGLDEFSMSAGSILKVRDVVRNTRYEDAKALAARAL 554 Query: 555 RHRTSTET 562 T + Sbjct: 555 EADTEADV 562 >B9MM71.1 B9MM71_ANATD Phosphoenolpyruvate-protein phosphotransferase Length = 550 Score = 511 bits (1317), Expect = e-143, Method: Compositional matrix adjust. Identities = 248/527 (47%), Positives = 373/527 (70%), Gaps = 4/527 (0%) Query: 15 KAFVLPNWEWSLPDTQVNPVDLAKEFERLYEGIRTSKDEIEFIKREFREVVGPEESSIFD 74 +A V+ E+++ T++ D E R + I +K++I IK +E +G + + IFD Sbjct: 16 RAVVIKESEYTIKKTKIEDTD--AELRRFLDSIEKAKEQIRKIKAATQESLGKKNAMIFD 73 Query: 75 AHLAILDDPVFMSEIRGIIERQYKAAEVAVKEAIDHFVAMFDLLDDEYMKERAVDIKDVG 134 AHL ILDDP F++ +RG IE A E A+ E+ F M L+DEYM+ER DIKDV Sbjct: 74 AHLLILDDPEFVNMVRGKIEEGINA-EFAIDESARFFENMLLSLEDEYMRERTNDIKDVA 132 Query: 135 NRLLKHLLGAPEVTLPSDTQPYILVAKELSPSQLAHLNPTYVLGIVTMMGGKTSHSSIMA 194 RL+K+L G ++ L + + IL+A +L+PSQ A +N V G VT GGKTSH++I+A Sbjct: 133 LRLIKNLNGEEQIDLKNLPEDSILIAHDLTPSQTAQINKQNVRGFVTEKGGKTSHTAIIA 192 Query: 195 RALGIPLVAGLENKILTPIQTGDLLVMDGETGVIQLYPDELTLKEYTSRRAKQQRKKEQL 254 R IP V G+E I+ I+ GD L++DG G + + P+E +KEY + ++ ++KE+L Sbjct: 193 RTYEIPAVVGVEG-IVNRIKDGDFLIVDGYEGFVYVNPEEDLIKEYEKKLDEENKRKEEL 251 Query: 255 ELLATVDAVTKDGVQLRLAGNISSVKELDMALKYGAQGVGLFRTEFLYMDRSSFPTEEEQ 314 + V++ T+DG +++L NI+ ++E+D ALK GA+G+GLFRTEFL+MDRS P+E+EQ Sbjct: 252 KSFLYVESKTQDGKRIKLFANIAHIEEIDAALKNGAEGIGLFRTEFLFMDRSQPPSEDEQ 311 Query: 315 FEVYKLVAEKVGNETVVIRTLDIGGDKHLDYFQLPDEQNPFLGYRAIRISLDRKDMFKTQ 374 FEVYK V EK+ + V+IRTLD+GGDK++ Y + E+NPFLGYRAIR+ L K++FKTQ Sbjct: 312 FEVYKTVLEKMEGKPVIIRTLDVGGDKNISYLNIDKEENPFLGYRAIRLCLGNKELFKTQ 371 Query: 375 MTAILRASHYGNIKMMFPMISSVEEVQAAKAVLNEVKEELDQQGVPYNRNIPVGIMIEVP 434 + A+LRAS YG +K+MFPMI+ ++EV AK ++ E KEEL ++ + +++NI +GIMIE P Sbjct: 372 LRALLRASIYGKLKIMFPMITCIDEVYQAKWIIQEAKEELKKENILFSQNIEIGIMIETP 431 Query: 435 AAVMIADLLAEEVDFFSIGTNDLVQYVLAVDRMNEQIAHMYHPYHPGVLRMIRMTVEAAR 494 AA +I+D+L +EVDFFSIGTNDL+QY LA+DR N++++++Y+P HP VLR+I+MTVE A Sbjct: 432 AAAVISDILDKEVDFFSIGTNDLIQYTLAIDRTNDKVSYLYNPLHPAVLRLIKMTVENAH 491 Query: 495 RVGIDISVCGEMAADERSLPLWLEMGISDLSMSPQALLKVKHRALNT 541 + GI++ VCGE+A+++ +P+ + +G+ +LS++P +L VK + L T Sbjct: 492 KRGIEVGVCGEIASNQEFVPVLIGLGVDELSVNPSKILNVKKKILQT 538 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,225,111,468 Number of extensions: 130852525 Number of successful extensions: 437597 Number of sequences better than 1.0e-05: 3215 Number of HSP's gapped: 429486 Number of HSP's successfully gapped: 3507 Length of query: 588 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 446 Effective length of database: 1,722,912,073 Effective search space: 768418784558 Effective search space used: 768418784558 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)