BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0027.p (175 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIZ2.1 B3KIZ2_9BACI Intracellular protease, PfpI family 308 9e-83 B1DHR4.1 B1DHR4_9BACL Intracellular protease, PfpI family 250 3e-65 Q2B655.1 Q2B655_9BACI Intracellular proteinase 248 1e-64 Q5WGC2.1 Q5WGC2_BACSK ThiJ/PfpI family intracellular protease 248 2e-64 A7Z833.1 A7Z833_BACA2 Putative uncharacterized protein 246 5e-64 >B3KIZ2.1 B3KIZ2_9BACI Intracellular protease, PfpI family Length = 176 Score = 308 bits (790), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 151/175 (86%), Positives = 164/175 (93%), Gaps = 1/175 (0%) Query: 1 MRLTGKKVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKAEYSW 60 MRLTGKKVIALVD+EFEDLELWYPVYRVREEGAEVHLAGLEK KTY GKYGVPA+AEYS+ Sbjct: 1 MRLTGKKVIALVDEEFEDLELWYPVYRVREEGAEVHLAGLEKGKTYTGKYGVPAQAEYSF 60 Query: 61 DELDAADYDGILVPGGWAPDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAKILE 120 +EL++ DYDGILVPGGWAPDK+RR VL+LV++ N +KPIGQICHAGWVLISAKIL+ Sbjct: 61 EELNSKDYDGILVPGGWAPDKLRRYDK-VLQLVKEMNEDQKPIGQICHAGWVLISAKILD 119 Query: 121 GVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDALAGE 175 GVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDALA + Sbjct: 120 GVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDALAQQ 174 >B1DHR4.1 B1DHR4_9BACL Intracellular protease, PfpI family Length = 176 Score = 250 bits (639), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 126/172 (73%), Positives = 143/172 (83%), Gaps = 1/172 (0%) Query: 1 MRLTGKKVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKAEYSW 60 M L K VI L++DEFEDLELWYPVYRVRE GA V AG EK K ++GKYGVPA A+ S+ Sbjct: 2 MSLHNKTVICLLEDEFEDLELWYPVYRVREAGATVLYAGPEKGKKHIGKYGVPAVADLSF 61 Query: 61 DELDAADYDGILVPGGWAPDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAKILE 120 DE+++AD G+LVPGGWAPDK+RR VL+LVQ+ +AA KPIGQICHAGWVLISAKIL+ Sbjct: 62 DEVNSADIAGLLVPGGWAPDKLRRYPK-VLQLVQELDAAGKPIGQICHAGWVLISAKILQ 120 Query: 121 GVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDAL 172 G VTSTPGIRDDMENAGA W DEAVV DG++ISARRPPDLPPY KAF DAL Sbjct: 121 GRKVTSTPGIRDDMENAGAEWFDEAVVVDGNLISARRPPDLPPYAKAFVDAL 172 >Q2B655.1 Q2B655_9BACI Intracellular proteinase Length = 174 Score = 248 bits (633), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 121/172 (70%), Positives = 142/172 (82%), Gaps = 1/172 (0%) Query: 1 MRLTGKKVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKAEYSW 60 MRL GKK+I+LV EFEDLELWYP+ R+REEGA V LAG ++TY+GKYGV AK++Y++ Sbjct: 1 MRLEGKKIISLVHHEFEDLELWYPILRLREEGAIVELAGETANETYLGKYGVQAKSDYAY 60 Query: 61 DELDAADYDGILVPGGWAPDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAKILE 120 E+ A DYD ILVPGGWAPDKIRR V+ L+++ + AKKPIGQICHAGWVLISAKILE Sbjct: 61 SEISADDYDAILVPGGWAPDKIRRFPE-VISLIREMDEAKKPIGQICHAGWVLISAKILE 119 Query: 121 GVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDAL 172 G TVTSTPGI+DDMENAGAIW DE VTDGHI+S+RRPPDLP Y + F AL Sbjct: 120 GRTVTSTPGIKDDMENAGAIWVDEPAVTDGHIVSSRRPPDLPDYMREFIRAL 171 >Q5WGC2.1 Q5WGC2_BACSK ThiJ/PfpI family intracellular protease Length = 174 Score = 248 bits (632), Expect = 2e-64, Method: Compositional matrix adjust. Identities = 119/174 (68%), Positives = 140/174 (80%), Gaps = 1/174 (0%) Query: 1 MRLTGKKVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKAEYSW 60 MRL GK+V+A VD EFEDLELWYPV R++EEGA+V AG E +KTY+GKYGVPA + ++ Sbjct: 1 MRLKGKRVLAAVDAEFEDLELWYPVIRLQEEGADVVFAGAEANKTYIGKYGVPATSTIAF 60 Query: 61 DELDAADYDGILVPGGWAPDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAKILE 120 +E ++DG+LVPGGWAPDK+RR L +++ + KKPIGQICHAGWVLISA IL Sbjct: 61 NEASTDEFDGVLVPGGWAPDKLRRYPE-FLSFIKEMDKQKKPIGQICHAGWVLISAGILN 119 Query: 121 GVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDALAG 174 G VTSTPGIRDDMENAGAIW DE VV DGH+IS+RRPPDL PYGKAF DALAG Sbjct: 120 GKRVTSTPGIRDDMENAGAIWHDEPVVVDGHVISSRRPPDLAPYGKAFADALAG 173 >A7Z833.1 A7Z833_BACA2 Putative uncharacterized protein Length = 174 Score = 246 bits (628), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 122/175 (69%), Positives = 138/175 (78%), Gaps = 1/175 (0%) Query: 1 MRLTGKKVIALVDDEFEDLELWYPVYRVREEGAEVHLAGLEKDKTYVGKYGVPAKAEYSW 60 MRL GKKVIALV D+FEDLELWYPVYR+REEGA VHL G E ++Y GKYGVPA A+Y + Sbjct: 1 MRLEGKKVIALVSDDFEDLELWYPVYRLREEGAIVHLIGEEAGRSYKGKYGVPATADYDF 60 Query: 61 DELDAADYDGILVPGGWAPDKIRRXXXAVLKLVQDFNAAKKPIGQICHAGWVLISAKILE 120 L +YD +LVPGGWAPDK+RR VL +++ N KKPIGQICHAGWVLISA IL Sbjct: 61 KSLIIQEYDAVLVPGGWAPDKLRRYPE-VLDIIRTMNEQKKPIGQICHAGWVLISAGILA 119 Query: 121 GVTVTSTPGIRDDMENAGAIWKDEAVVTDGHIISARRPPDLPPYGKAFCDALAGE 175 G VTSTPGI+DDM NAGA W DEAVVTDGHI+S+RRPPDLP Y KAF D LA + Sbjct: 120 GKKVTSTPGIKDDMTNAGAQWLDEAVVTDGHIVSSRRPPDLPQYAKAFADLLASK 174 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.139 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,129,686,749 Number of extensions: 45428771 Number of successful extensions: 98784 Number of sequences better than 1.0e-05: 1128 Number of HSP's gapped: 97651 Number of HSP's successfully gapped: 1144 Length of query: 175 Length of database: 3,060,696,203 Length adjustment: 126 Effective length of query: 49 Effective length of database: 1,873,648,313 Effective search space: 91808767337 Effective search space used: 91808767337 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)