BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0024.p (231 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIZ6.1 B3KIZ6_9BACI Adenosylhomocysteine nucleosidase EC=3.2.2.9 337 5e-91 C0ZHL6.1 C0ZHL6_BREBN Probable MTA/SAH nucleosidase EC=3.2.2.9 281 2e-74 B1DHR0.1 B1DHR0_9BACL Adenosylhomocysteine nucleosidase EC=3.2.2.9 280 1e-73 Q79B62.1 Q79B62_BACPF 5'-methylthioadenosine/S-adenosylhomocyste... 233 1e-59 Q9K7X0.1 Q9K7X0_BACHD 5'-methylthioadenosine/S-adenosylhomocyste... 218 3e-55 >B3KIZ6.1 B3KIZ6_9BACI Adenosylhomocysteine nucleosidase EC=3.2.2.9 Length = 233 Score = 337 bits (864), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 162/228 (71%), Positives = 193/228 (84%), Gaps = 1/228 (0%) Query: 2 SEIIGLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVTT 61 +++IGLIGAMDEE++LLL +E+K +TV+AGV Y SGTI G++VV+CKSGVGKVNAAVTT Sbjct: 3 TQVIGLIGAMDEEVELLLGQLENKETTVRAGVTYASGTIHGKQVVVCKSGVGKVNAAVTT 62 Query: 62 QILIDAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIPYQEVSS 121 QILID FGV+++LFTGVAGA+HP+L IGDIVISS C+QHDMDVT LGY RGVIPYQE+S Sbjct: 63 QILIDFFGVSKILFTGVAGALHPDLNIGDIVISSSCMQHDMDVTPLGYARGVIPYQELSD 122 Query: 122 FPAAPDLVQLAEEACRALK-QKSVTGVVLSGDQFIASAAVVAELREQLDGACAEMEGAAV 180 FPA L+ LAEEAC+ + G VLSGDQFIAS V L E+L+GACAEMEG+AV Sbjct: 123 FPADDSLIHLAEEACKEQAVNHYIVGKVLSGDQFIASRDTVQTLYEELNGACAEMEGSAV 182 Query: 181 AQVCYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERSHAILEYML 228 AQVCYMNG+P+V+IRSMSDKADGSAHVN+ EFTV AS+RSH I+ YML Sbjct: 183 AQVCYMNGVPYVVIRSMSDKADGSAHVNFPEFTVKASKRSHEIVNYML 230 >C0ZHL6.1 C0ZHL6_BREBN Probable MTA/SAH nucleosidase EC=3.2.2.9 Length = 310 Score = 281 bits (720), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 140/227 (61%), Positives = 174/227 (76%), Gaps = 2/227 (0%) Query: 6 GLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVTTQILI 65 G+IGAMDEEI L LE+M++ + KAG+ YY+G + G+ VVLCKSGVGKVNAAVTTQILI Sbjct: 4 GIIGAMDEEIALYLEAMQETTTATKAGITYYTGKMEGKDVVLCKSGVGKVNAAVTTQILI 63 Query: 66 DAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIPYQEVSSFPAA 125 D+F V RV+FTGVAGAVHPEL IGDIV+S+ CIQHD+DVT LG+ G IP+ E ++ A Sbjct: 64 DSFQVERVIFTGVAGAVHPELNIGDIVVSTDCIQHDIDVTPLGFEPGQIPFTEQWTWQAD 123 Query: 126 PDLVQLAEEACRALKQ--KSVTGVVLSGDQFIASAAVVAELREQLDGACAEMEGAAVAQV 183 L+Q A +A + L+ + V+G +LSGDQF+AS V L EQ C EMEGA+V QV Sbjct: 124 AGLMQQAIDAGKELEAGVQVVSGRILSGDQFVASREKVQWLYEQFAAHCTEMEGASVGQV 183 Query: 184 CYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERSHAILEYMLKA 230 C MNG+PFV++RSMSDKADGSAHVN+ EFT AS+RS+AI+ ML A Sbjct: 184 CAMNGVPFVVVRSMSDKADGSAHVNFVEFTKLASQRSYAIVRNMLTA 230 >B1DHR0.1 B1DHR0_9BACL Adenosylhomocysteine nucleosidase EC=3.2.2.9 Length = 232 Score = 280 bits (715), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 144/226 (63%), Positives = 172/226 (76%), Gaps = 2/226 (0%) Query: 5 IGLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVTTQIL 64 IG+IGAM EEI+LL +++ S KAG+ YY G + + VV CKSGVGKVNAAV TQIL Sbjct: 6 IGIIGAMVEEIELLHQNVNVTNSVTKAGITYYEGQLHEKTVVFCKSGVGKVNAAVCTQIL 65 Query: 65 IDAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIPYQEVSSFPA 124 ID GV VLFTGVAGA+ P L IGDIV+SS C+QHDMDVT LG+ +GVIPYQE S F A Sbjct: 66 ID-LGVDSVLFTGVAGALDPRLNIGDIVVSSSCVQHDMDVTPLGFAKGVIPYQERSEFTA 124 Query: 125 APDLVQLAEEAC-RALKQKSVTGVVLSGDQFIASAAVVAELREQLDGACAEMEGAAVAQV 183 LV+LA EA R + +S+ G VLSGDQFIAS V EL E + GACAEMEGA+VA V Sbjct: 125 DDRLVKLASEASERLFEGRSLIGKVLSGDQFIASRDTVRELYENMQGACAEMEGASVAHV 184 Query: 184 CYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERSHAILEYMLK 229 C +N +PFV+IRSMSDKADGSAHVNY EFTVTA+ S+AI++ M++ Sbjct: 185 CDLNEVPFVVIRSMSDKADGSAHVNYAEFTVTAANNSYAIIDDMIQ 230 >Q79B62.1 Q79B62_BACPF 5'-methylthioadenosine/S-adenosylhomocysteine EC=3.2.2.16 Length = 233 Score = 233 bits (593), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 119/229 (51%), Positives = 164/229 (71%), Gaps = 2/229 (0%) Query: 5 IGLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVTTQIL 64 + +IGAM+EEI+ + + M + + A V +Y G + G +VLCKSGVGKVNAA+TTQIL Sbjct: 3 VAVIGAMNEEIERMQKEMSVEETITFAKVPFYVGKMCGIDIVLCKSGVGKVNAALTTQIL 62 Query: 65 IDAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIP-YQEVSSFP 123 ID F V+ +LFTGVAGA+ L +GD+VIS+ +QHD+D + LG+ RG IP Y S F Sbjct: 63 IDRFEVSHILFTGVAGALDESLEVGDLVISTSAMQHDLDASPLGFKRGEIPMYDGDSDFK 122 Query: 124 AAPDLVQLAEEACRALKQKSVT-GVVLSGDQFIASAAVVAELREQLDGACAEMEGAAVAQ 182 A+ LV++A+EA +L + V G +LSGDQFIA+ V +L+E +G C EMEGA+VA Sbjct: 123 ASTRLVEIAKEAADSLTDRQVVCGRILSGDQFIANKEEVKQLKELFNGVCVEMEGASVAH 182 Query: 183 VCYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERSHAILEYMLKAM 231 V MN +PFVIIRSMSDKA+G A ++++EFT ASE+SH +++ MLK + Sbjct: 183 VSDMNDVPFVIIRSMSDKANGEAQISFQEFTDIASEQSHLLVKAMLKEL 231 >Q9K7X0.1 Q9K7X0_BACHD 5'-methylthioadenosine/S-adenosylhomocysteine nuclosidase EC=3.2.2.16 Length = 233 Score = 218 bits (555), Expect = 3e-55, Method: Compositional matrix adjust. Identities = 120/229 (52%), Positives = 154/229 (67%), Gaps = 2/229 (0%) Query: 5 IGLIGAMDEEIKLLLESMEDKVSTVKAGVAYYSGTIFGQRVVLCKSGVGKVNAAVTTQIL 64 IGLIGAM EE+ L+ E M+ + A V +Y GTI G RVVL KSGVGKVNAA+TTQ+L Sbjct: 4 IGLIGAMKEELALIKEQMDVISESTLATVCFYEGTIHGARVVLVKSGVGKVNAAMTTQLL 63 Query: 65 IDAFGVARVLFTGVAGAVHPELGIGDIVISSHCIQHDMDVTALGYPRGVIP-YQEVSSFP 123 ID F V ++FTGVAGA+ P L + D+VIS+ HDMD T LG+ RG IP ++ S F Sbjct: 64 IDHFHVDAIIFTGVAGALSPSLEVADLVISTSLQHHDMDGTPLGFKRGEIPMFERSSDFL 123 Query: 124 AAPDLVQLAEE-ACRALKQKSVTGVVLSGDQFIASAAVVAELREQLDGACAEMEGAAVAQ 182 A L +A + A +K V G ++SGDQFIA V EL E+ D C EMEGAAVAQ Sbjct: 124 ADEWLASVAYDVATNETGRKVVKGRIVSGDQFIADRHRVRELAEKFDAVCVEMEGAAVAQ 183 Query: 183 VCYMNGIPFVIIRSMSDKADGSAHVNYREFTVTASERSHAILEYMLKAM 231 V +N IPFV+IRS+SDKA+ A++++ EFT ASE+S I+ +LK + Sbjct: 184 VAALNQIPFVVIRSISDKANEEANISFAEFTKIASEQSQLIVSGVLKKL 232 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,230,120,565 Number of extensions: 46912910 Number of successful extensions: 127140 Number of sequences better than 1.0e-05: 1294 Number of HSP's gapped: 125955 Number of HSP's successfully gapped: 1294 Length of query: 231 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 100 Effective length of database: 1,826,543,238 Effective search space: 182654323800 Effective search space used: 182654323800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)