BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0023.p (210 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIZ7.1 B3KIZ7_9BACI GCN5-related N-acetyltransferase 303 4e-81 B1DHQ8.1 B1DHQ8_9BACL Acetoin dehydrogenase 294 4e-78 B4BPT8.1 B4BPT8_9BACI Acetoin utilization protein 256 8e-67 C5D6A4.1 C5D6A4_9BACI Acetoin utilization protein 254 4e-66 B7GGP3.1 B7GGP3_ANOFW Acetyltransferase (GNAT family), involved ... 253 8e-66 >B3KIZ7.1 B3KIZ7_9BACI GCN5-related N-acetyltransferase Length = 210 Score = 303 bits (777), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 145/211 (68%), Positives = 169/211 (80%), Gaps = 2/211 (0%) Query: 1 MEHRKIPVSHIL-PHHRSLITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLP 59 MEH K+ SH+L P R LI + GP+ P+ L L MHPDL AFR+P EQ EAL+EIAGLP Sbjct: 1 MEHCKVYQSHVLFPEDRRLI-IEGPVPPEQLSELTMHPDLKAFRRPAEQHEALVEIAGLP 59 Query: 60 EGRVIAAVVDQVIAGYVTFHYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITS 119 EGR+I +I GYVTFH+PDE E WS+G MEDLIELGA+EVA +R +GL + +I + Sbjct: 60 EGRIIITRDRDMIVGYVTFHHPDEQERWSEGNMEDLIELGAIEVAKDYRSLGLGQRMIKT 119 Query: 120 AFEREQLENCIVFTTEYYWHWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHP 179 AFE Q+E IVFTTEYYWHWDLKGSGL+VW YR+MME+LM+ VDM WYATDDPEICSHP Sbjct: 120 AFEEGQMERYIVFTTEYYWHWDLKGSGLNVWDYRKMMERLMKVVDMEWYATDDPEICSHP 179 Query: 180 ANCLMVRIGREVPMSSQETFDRVRFRQRFMY 210 ANCLMVRIGR VP+SSQE FDR+RFRQRFMY Sbjct: 180 ANCLMVRIGRHVPLSSQEQFDRIRFRQRFMY 210 >B1DHQ8.1 B1DHQ8_9BACL Acetoin dehydrogenase Length = 211 Score = 294 bits (752), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 138/211 (65%), Positives = 165/211 (78%), Gaps = 1/211 (0%) Query: 1 MEHRK-IPVSHILPHHRSLITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLP 59 MEHRK I + + GP+ P++LQ L MH DLDAFR+P++Q EAL+EIAGL Sbjct: 1 MEHRKKYQRQQIELQDGRICIIEGPVPPESLQTLAMHADLDAFRRPKDQHEALVEIAGLE 60 Query: 60 EGRVIAAVVDQVIAGYVTFHYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITS 119 EGR+I A +D++I GYVTFHYPDELE W + GM DL+ELGA+EVAD +R GL K +I Sbjct: 61 EGRIILARLDELIVGYVTFHYPDELERWYEAGMPDLVELGAIEVADQYRSYGLGKKMIQV 120 Query: 120 AFEREQLENCIVFTTEYYWHWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHP 179 AF EQLEN IVFTTEYYWHWDL+G+ L VW YR +MEKLM++V MVWYATDDPEICSHP Sbjct: 121 AFADEQLENMIVFTTEYYWHWDLEGTKLSVWEYRGIMEKLMKSVGMVWYATDDPEICSHP 180 Query: 180 ANCLMVRIGREVPMSSQETFDRVRFRQRFMY 210 ANCLMVRIG+E+P S+E FDR+RFRQRFMY Sbjct: 181 ANCLMVRIGKEIPQESEEQFDRIRFRQRFMY 211 >B4BPT8.1 B4BPT8_9BACI Acetoin utilization protein Length = 210 Score = 256 bits (654), Expect = 8e-67, Method: Compositional matrix adjust. Identities = 119/210 (56%), Positives = 152/210 (72%) Query: 1 MEHRKIPVSHILPHHRSLITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLPE 60 MEH+K + L + + + GP++P+AL + HPDL +FR+P +Q EALIEIA LPE Sbjct: 1 MEHKKTYNAKELKTPKGTLLIEGPVTPEALASYEFHPDLTSFRQPSKQHEALIEIAKLPE 60 Query: 61 GRVIAAVVDQVIAGYVTFHYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITSA 120 GR+I A I GYVTF YPD LE WS+G ME+LIELGA+EV FRG G+ K L+ + Sbjct: 61 GRIIIARRGGTIVGYVTFLYPDPLERWSEGKMENLIELGAIEVIPEFRGFGVGKNLLIVS 120 Query: 121 FEREQLENCIVFTTEYYWHWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHPA 180 + +E+ I+ TTEYYWHWDLKG+GL+VW YR++MEK+M +VWYATDDPEICSHPA Sbjct: 121 MMDDAMEDYIIITTEYYWHWDLKGTGLNVWEYRKVMEKMMNAGGLVWYATDDPEICSHPA 180 Query: 181 NCLMVRIGREVPMSSQETFDRVRFRQRFMY 210 NCLMVRIG+ V S + FDR+RF R+MY Sbjct: 181 NCLMVRIGKRVDPESIQRFDRLRFMNRYMY 210 >C5D6A4.1 C5D6A4_9BACI Acetoin utilization protein Length = 210 Score = 254 bits (648), Expect = 4e-66, Method: Compositional matrix adjust. Identities = 118/210 (56%), Positives = 151/210 (71%) Query: 1 MEHRKIPVSHILPHHRSLITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLPE 60 MEH+K + L + + + GP+S + L + H DL +FR+P +Q +ALIEIA LPE Sbjct: 1 MEHKKTYNAKELKTPKGTLIIEGPVSAEKLASYEFHHDLTSFRQPPQQHKALIEIAKLPE 60 Query: 61 GRVIAAVVDQVIAGYVTFHYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITSA 120 GR+I A +Q I GYVTF YPD LE WSQG ME+LIELGA+EV FRG G+ K L+ + Sbjct: 61 GRIIIARHNQTIVGYVTFLYPDPLERWSQGNMENLIELGAIEVIPEFRGYGVGKNLLIVS 120 Query: 121 FEREQLENCIVFTTEYYWHWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHPA 180 + +E+ I+ TTEYYWHWDLKG+GL+VW YR++MEK+M +VWYATDDPEICSHPA Sbjct: 121 MMDDAMEDYIIITTEYYWHWDLKGTGLNVWEYRKLMEKMMNAGGLVWYATDDPEICSHPA 180 Query: 181 NCLMVRIGREVPMSSQETFDRVRFRQRFMY 210 NCLMVRIG+ V S + FDR+RF R+MY Sbjct: 181 NCLMVRIGKRVDQESIQKFDRLRFMNRYMY 210 >B7GGP3.1 B7GGP3_ANOFW Acetyltransferase (GNAT family), involved in acetoin utilization Length = 210 Score = 253 bits (646), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 119/210 (56%), Positives = 148/210 (70%) Query: 1 MEHRKIPVSHILPHHRSLITVSGPLSPDALQGLQMHPDLDAFRKPQEQLEALIEIAGLPE 60 MEH+K + L + + + GP+SP+ L + H DL AFR+P +Q ALIEIAGLPE Sbjct: 1 MEHKKTYNAKQLKTPKGTLIIEGPISPEKLASYEFHHDLTAFRQPHQQHRALIEIAGLPE 60 Query: 61 GRVIAAVVDQVIAGYVTFHYPDELELWSQGGMEDLIELGAVEVADGFRGIGLAKLLITSA 120 GR+I A I GYVTF YPD LE WSQG ME+LIELGA+EV FRG G+ K L+ A Sbjct: 61 GRIIIARHGHTIVGYVTFLYPDPLERWSQGKMENLIELGAIEVIPDFRGCGVGKNLLKVA 120 Query: 121 FEREQLENCIVFTTEYYWHWDLKGSGLDVWAYRQMMEKLMQTVDMVWYATDDPEICSHPA 180 + +E+ I+ TTEYYWHWDLK +GL+VW YR++MEK+M +VWYATDDPEICSHPA Sbjct: 121 MMDDAMEDYIIITTEYYWHWDLKRTGLNVWEYRKVMEKMMNAGGLVWYATDDPEICSHPA 180 Query: 181 NCLMVRIGREVPMSSQETFDRVRFRQRFMY 210 NCLM RIG+ V S + FD++RF RFMY Sbjct: 181 NCLMARIGKRVDQQSIQKFDQLRFMNRFMY 210 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.323 0.139 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,328,208,747 Number of extensions: 54522494 Number of successful extensions: 127165 Number of sequences better than 1.0e-05: 131 Number of HSP's gapped: 127160 Number of HSP's successfully gapped: 131 Length of query: 210 Length of database: 3,060,696,203 Length adjustment: 130 Effective length of query: 80 Effective length of database: 1,835,964,253 Effective search space: 146877140240 Effective search space used: 146877140240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)