BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0018.p (521 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ01.1 B3KJ01_9BACI Diguanylate cyclase 654 0.0 B1DHP9.1 B1DHP9_9BACL Diguanylate cyclase with GAF sensor 528 e-148 C0ZHK6.1 C0ZHK6_BREBN Putative uncharacterized protein 359 4e-97 B7GGP8.1 B7GGP8_ANOFW Signal transduction diguanylate cyclase (G... 299 5e-79 C1P9Q5.1 C1P9Q5_BACCO Diguanylate cyclase 282 7e-74 >B3KJ01.1 B3KJ01_9BACI Diguanylate cyclase Length = 663 Score = 654 bits (1687), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/505 (62%), Positives = 391/505 (77%) Query: 11 LYIAEAMSLHFQACFYRRFEHMFMSDLAGLHLHAERESSRRSLLFQIVQRMHDNIDVDAV 70 L + E +L F++CFYR+ E++F DL +H AERE RRS+LFQ V+RMH I+VD+V Sbjct: 155 LMLTETYALLFRSCFYRQMEYIFADDLEKVHRQAEREVQRRSILFQYVKRMHVQINVDSV 214 Query: 71 LTEVIDSISAMYPGARLELFMSQDHRSANPLVKPLPLHWGDDDVCARAFKDGRVSLYAGR 130 L E IDS+ A+YP A++ELFMSQDH S +PLV+PL LH ++V R F +G ++++ Sbjct: 215 LVEAIDSVLALYPKAKVELFMSQDHESVHPLVRPLLLHHWQEEVYVRTFMEGSLTIHEHE 274 Query: 131 EDNHTVEIGLPLGGKQGVYGVFHMVMDKPAFPEVDLRFLSMVADTAGTAFENAKLYERSN 190 E + VEIG+P+GGKQGVYGV H+ +DK P++DL L M+AD AGTAFENAKLYE+SN Sbjct: 275 EPDTLVEIGIPMGGKQGVYGVLHVEVDKSELPDMDLELLGMMADAAGTAFENAKLYEQSN 334 Query: 191 QLIRELRMSNELTQRLNQSLRLGDIFQFAFEELLEMFDADYCCILHMNEDKGGLEAIACN 250 +I ELRM NELTQRLN+SL L +IFQFA ELL++ +ADYCCIL NE+ GGLE ++CN Sbjct: 335 LMIHELRMINELTQRLNKSLHLAEIFQFANHELLKILEADYCCILIHNEELGGLEVVSCN 394 Query: 251 YHPLQXXXXXXXXXXXXRVYTTGEALILSDYRNADGSYSRLMNATGSNSVIATPLNVGGE 310 L VY + E LILSDY+ + SRLM +T S+S+IATPL V GE Sbjct: 395 AEWLAKEVFEKDYGLGGLVYHSKEPLILSDYQEERPAESRLMESTRSSSMIATPLMVNGE 454 Query: 311 VRGAIMLTHREPHFFSYDSYRLLQAMAGHIGLAVGNARLHAEVRRLANRDSLTGLYARHY 370 VRG ++LTH + H+FSYDSYRLLQA+ HIGLA+GNA LHAEVRR+ANRD LT LYARHY Sbjct: 455 VRGVVLLTHAKRHYFSYDSYRLLQALTSHIGLAIGNAFLHAEVRRMANRDMLTELYARHY 514 Query: 371 LDEIIKDKQITEFCGSLIVVDIDQFKMVNDTYGHQKGDKILRQVSEIVKSSIRQGDIAAR 430 LDEII + Q +FCGSLIVVDID+FK VNDT+GHQ+GDKIL+QVS IVK++IR DI AR Sbjct: 515 LDEIIHECQNRDFCGSLIVVDIDEFKQVNDTFGHQRGDKILKQVSSIVKTTIRHSDIPAR 574 Query: 431 WGGEELAVYLPQLGVQQAVFVAERIRKQVMSETDPRVTVSCGIAEWSWTDDKVSVESLFY 490 WGGEELAVYLP LG+QQAV VAERIR +V ETDPRVTVSCGI+EW+WT+DK+SVESLFY Sbjct: 575 WGGEELAVYLPGLGIQQAVQVAERIRLRVAEETDPRVTVSCGISEWNWTNDKISVESLFY 634 Query: 491 RADMALYEAKNNGRNQVVLDTKIAE 515 RADMALYEAKN GRN++V++ AE Sbjct: 635 RADMALYEAKNGGRNRIVVERAEAE 659 >B1DHP9.1 B1DHP9_9BACL Diguanylate cyclase with GAF sensor Length = 653 Score = 528 bits (1361), Expect = e-148, Method: Compositional matrix adjust. Identities = 256/497 (51%), Positives = 354/497 (71%), Gaps = 2/497 (0%) Query: 13 IAEAMSLHFQACFYRRFEHMFMSDLAGLHLHAERESSRRSLLFQIVQRMHDNIDVDAVLT 72 IA+ +LHF+ CFYR+FE +F+ DL ++E+SRR LF +R++D DV +VL+ Sbjct: 155 IAQLSALHFRTCFYRKFEQLFIKDLLLQQKRTDKEASRRDALFLAAKRLYDQFDVTSVLS 214 Query: 73 EVIDSISAMYPGARLELFMSQDHRSANPLVKPLPLHWGDDDVCARAFKDGRVSLYAGRED 132 E++ S+ +YP + +L++SQDH + + VKPL D+ A++F DG+ L ++ Sbjct: 215 EMLSSLEQLYPNSVAQLYLSQDHVNGDSRVKPLVFKNAAHDLVAKSFLDGK--LVTEQDR 272 Query: 133 NHTVEIGLPLGGKQGVYGVFHMVMDKPAFPEVDLRFLSMVADTAGTAFENAKLYERSNQL 192 + T + +P+ GKQ YGV + ++ ++ + DL ++ADTAGTAFENAKLYE+SN L Sbjct: 273 DGTKRLAVPMTGKQAAYGVLCLTVNASSWDDADLPAFVLLADTAGTAFENAKLYEQSNLL 332 Query: 193 IRELRMSNELTQRLNQSLRLGDIFQFAFEELLEMFDADYCCILHMNEDKGGLEAIACNYH 252 I ELR+ NELT+RLNQSL+L +IF+FA ELL +FDADYCC+L +N++K +A N Sbjct: 333 INELRLINELTKRLNQSLKLKEIFKFASSELLSVFDADYCCVLQLNKEKNQFVVMASNIP 392 Query: 253 PLQXXXXXXXXXXXXRVYTTGEALILSDYRNADGSYSRLMNATGSNSVIATPLNVGGEVR 312 + V+ T E LI+SDY + S+LM+ TG+ S+IATP+ V EV Sbjct: 393 AMSSEHFSPEYGFCGIVHRTKEPLIISDYWHTRVVNSKLMDNTGARSLIATPILVDSEVV 452 Query: 313 GAIMLTHREPHFFSYDSYRLLQAMAGHIGLAVGNARLHAEVRRLANRDSLTGLYARHYLD 372 G IM+ H+ +FFSYD+Y+LLQ M+ HIGLA+ NA LHAEVRR+ D+LTGL+ARHYL+ Sbjct: 453 GVIMVAHKSANFFSYDNYKLLQVMSTHIGLAITNASLHAEVRRMVITDNLTGLHARHYLN 512 Query: 373 EIIKDKQITEFCGSLIVVDIDQFKMVNDTYGHQKGDKILRQVSEIVKSSIRQGDIAARWG 432 E I+ +Q + G+LI+VDID FK +NDT+GHQ GD+IL QVS I++SSIRQGDIAARWG Sbjct: 513 EQIQSRQRKDPLGALILVDIDHFKQINDTFGHQVGDQILVQVSSIIRSSIRQGDIAARWG 572 Query: 433 GEELAVYLPQLGVQQAVFVAERIRKQVMSETDPRVTVSCGIAEWSWTDDKVSVESLFYRA 492 GEELAVYLP + +QA +AERIR V +ET P VTVSCG++EW++ ++K+SVESLFYRA Sbjct: 573 GEELAVYLPGIRSEQAYRIAERIRTHVEAETQPVVTVSCGVSEWTFENEKISVESLFYRA 632 Query: 493 DMALYEAKNNGRNQVVL 509 DMALYEAKNNGRN++ + Sbjct: 633 DMALYEAKNNGRNRIYI 649 >C0ZHK6.1 C0ZHK6_BREBN Putative uncharacterized protein Length = 746 Score = 359 bits (922), Expect = 4e-97, Method: Compositional matrix adjust. Identities = 193/467 (41%), Positives = 288/467 (61%), Gaps = 8/467 (1%) Query: 46 RESSRRSLLFQIVQRMHDNIDVDAVLTEVIDSISAMYPGARLELFMSQDHRSANPLVKPL 105 RE+S++ LL+Q++ ++ +IDV+ VL E++ SI ++YP ELF++ + + P VK L Sbjct: 284 REASKKDLLYQLMSKIQASIDVNDVLHEIVTSIPSLYPYLSAELFLTVEPNATTP-VKQL 342 Query: 106 PLHWGDDDVCARAFKDGR-VSLYAGREDNHTVEIGLPLGGKQGVYGVFHMVMD-KPAFPE 163 + +A+ +GR ++ G + I PL GKQG+YGV + D + + Sbjct: 343 SFQEVEPSTSTKAYLEGRLIAEEVGEGEQQVTVIAAPLLGKQGIYGVLQVTSDQRITLNQ 402 Query: 164 VDLRFLSMVADTAGTAFENAKLYERSNQLIRELRMSNELTQRLNQSLRLGDIFQFAFEEL 223 + ++S++ADTAGTAFENA+LY++S LIRELR+ NE+ ++LN+SL L +I F + Sbjct: 403 HEKDYISILADTAGTAFENAQLYQQSRNLIRELRLINEMARQLNRSLDLKEILDFVTTMM 462 Query: 224 LEMFDADYCCILHMNEDKGGLEAIACNYHPLQXXXXXXXXXXXXRVYTTGEALILSDYRN 283 FDA++C IL + ++ + ++ +AL+L+ Sbjct: 463 RATFDAEFCAILRKTPGEDCFHVLSSSIPAFNGKVIDSKEKPFQQILQNKQALLLAQ--- 519 Query: 284 ADGSYSRLMNATGSNSVIATPLNVGGEVRGAIMLTHREPHFFSYDSYRLLQAMAGHIGLA 343 G+ + S + PL V GE+ G +++ HFFS+D Y+LL+ H LA Sbjct: 520 -PGAGPLPFSLFSCCSFMGVPLVVEGEISGVLLVADSRYHFFSFDDYKLLEIFGQHASLA 578 Query: 344 VGNARLHAEVRRLANRDSLTGLYARHYLDEIIKDKQITEFCGSLIVVDIDQFKMVNDTYG 403 + NA LH E+ R+ D+LTGLY R +L+E ++ + GSLI++DID FK VNDT+G Sbjct: 579 MTNAVLHNEMERMVITDNLTGLYTRRHLNERVRGSLEKDNYGSLILIDIDYFKTVNDTFG 638 Query: 404 HQKGDKILRQVSEIVKSSIRQGDIAARWGGEELAVYLPQLGVQQAVFVAERIRKQVMSET 463 HQ GD++L QVS +++ SIR DIAARWGGEELAVYLP++ A VAERIR+ V ET Sbjct: 639 HQVGDEVLIQVSNLIRHSIRDSDIAARWGGEELAVYLPRVDKNTAHSVAERIRECVEQET 698 Query: 464 DPRVTVSCGIAEWS-WTDDKVSVESLFYRADMALYEAKNNGRNQVVL 509 P+VT+SCG+A+WS D +SVE+LF++AD+ALYEAKN+GRNQVVL Sbjct: 699 SPQVTISCGLAKWSREMDVNLSVEALFHQADIALYEAKNSGRNQVVL 745 >B7GGP8.1 B7GGP8_ANOFW Signal transduction diguanylate cyclase (GAF-GAF-GGDEF domains) Length = 618 Score = 299 bits (765), Expect = 5e-79, Method: Compositional matrix adjust. Identities = 171/469 (36%), Positives = 272/469 (57%), Gaps = 19/469 (4%) Query: 47 ESSRRSLLFQIVQRMHDNIDVDAVLTEVIDSISAMYPGARLELFMSQDHRSANPL-VKPL 105 E R L + ++H ++++ VL E++ ++ +YP LF+S D + L +K L Sbjct: 160 EEKRYEQLHRAATKIHASMNIGDVLEEIVKTLKEVYPTFTCHLFLSYDSTTDRDLPIKLL 219 Query: 106 PLHWGDDDVCA-RAFKDGRVSLYAGREDNHTVEIGLPLGGKQGVYGVFHMVMDKPA-FPE 163 D+ + A +A+ G++ + +V I P+ G QG+YGV M+ PE Sbjct: 220 TFENEDEHMGAMQAYVTGQIQFHDSLVHKKSV-IYAPIKGAQGIYGVIEMIAPTVMELPE 278 Query: 164 VDLRFLSMVADTAGTAFENAKLYERSNQLIRELRMSNELTQRLNQSLRLGDIFQFAFEEL 223 ++RF+S++A+TAG+A ENAKLYE+S +LI +L++ NE LN +LR D QF E++ Sbjct: 279 REMRFISLLANTAGSALENAKLYEQSKRLIADLQLINETIHHLNTNLRFQDALQFMIEKI 338 Query: 224 LEMFDADYCCILHMNEDKGGLEAIACNYHPLQXXXXXXXXXXXXRVYTTGEALILSDYRN 283 FDA+ + + E + + + ++ R+ + L L D + Sbjct: 339 KTSFDAEEVGFIILQEKERKVLPGSTSFFQ-SREAKAYVDFVFHRIQHPRDTLFLGDVK- 396 Query: 284 ADGSYSRLMNATGSN---SVIATPLNVGGEVRGAIMLTHREPHFFSYDSYRLLQAMAGHI 340 +++ G+ S++A P+ + G ++G ++ H+E + FS+D ++LLQ++ H Sbjct: 397 --------IHSNGTKHFRSLMAVPM-MRGMMKGVAIVLHQEAYHFSFDMFKLLQSLIHHS 447 Query: 341 GLAVGNARLHAEVRRLANRDSLTGLYARHYLDEIIKDKQITEFCGSLIVVDIDQFKMVND 400 LA NA L E+ R+ D LT LYARHYLDE I+ + G+ I++DID FK VND Sbjct: 448 TLAFTNAALREELERMVITDRLTNLYARHYLDERIQRSMEEDGLGTFILIDIDNFKKVND 507 Query: 401 TYGHQKGDKILRQVSEIVKSSIRQGDIAARWGGEELAVYLPQLGVQQAVFVAERIRKQVM 460 TYGHQ GD+I+ QV+ I+K++IRQ DI ARWGGEELA+YLP++ + V +A R+ ++V Sbjct: 508 TYGHQVGDEIIIQVANIIKANIRQNDIGARWGGEELAIYLPKVSIHAGVSIANRLVQKVR 567 Query: 461 SETDPRVTVSCGIAEWSWTDDKVSVESLFYRADMALYEAKNNGRNQVVL 509 T+P VT+SCG++ W + SV+ LF RAD ALY AK G+N+VV+ Sbjct: 568 ELTNPPVTISCGVSYWK-KNRADSVKELFNRADEALYTAKRTGKNRVVV 615 >C1P9Q5.1 C1P9Q5_BACCO Diguanylate cyclase Length = 622 Score = 282 bits (721), Expect = 7e-74, Method: Compositional matrix adjust. Identities = 165/470 (35%), Positives = 273/470 (58%), Gaps = 15/470 (3%) Query: 44 AERESSRRSLLFQIVQRMHDNIDVDAVLTEVIDSISAMYPGARLELFMSQD-HRSANPLV 102 E + R LFQ+ + H ++D D +L E+I ++ +YP L +S D H + + + Sbjct: 156 VEADRKRYIELFQVTGKFHSSMDTDTILAEIIYTLKRLYPVFEYTLMLSDDYHHNLDLPI 215 Query: 103 KPLPLHWGDDDVCARAFKDGRVSLYAGREDNHTVEIGLPLGGKQGVYGVFHMV-MDKPAF 161 K L + D++ ++ +G+V L N T+ + PL GKQGVYGV ++ F Sbjct: 216 KSLQ-YDAVDEMALESYVNGQVHLATVWRTNETM-LYAPLRGKQGVYGVLQVITFANYEF 273 Query: 162 PEVDLRFLSMVADTAGTAFENAKLYERSNQLIRELRMSNELTQRLNQSLRLGDIFQFAFE 221 DL F+S++A+TAG+A ENAKLY++S Q I ELR+ N ++++LN+S L + F E Sbjct: 274 NRSDLEFISLLANTAGSALENAKLYQQSMQSIEELRLINSVSRQLNKSANLKETVDFLME 333 Query: 222 ELLEMFDADYCCILHMNEDKGGLEAIACNYHPLQXXXXXXXXXXXXRVYT-TGEALILSD 280 ++ F+ + M + G ++ + + + R + T E+L +S+ Sbjct: 334 QISSSFNTQAIGFVMM--ENGKMDLLEGSSPIFEEKEGIRYLDFANRSFADTKESLFISN 391 Query: 281 Y-RNADGSYSRLMNATGSNSVIATPLNVGGEVRGAIMLTHREPHFFSYDSYRLLQAMAGH 339 + G R +S++A P+ +++G ++ H P+ FS+D ++L Q++ Sbjct: 392 LNQRFPGRNYRF------SSLMAVPIIQEDDLKGFCLVLHEAPYHFSFDRFKLFQSLIYQ 445 Query: 340 IGLAVGNARLHAEVRRLANRDSLTGLYARHYLDEIIKDKQITEFCGSLIVVDIDQFKMVN 399 G+A+ N+ L + ++ D LT L+A++YL+ ++ + G+LI++DID FK VN Sbjct: 446 SGMALTNSMLRERLEKMVITDHLTQLFAKNYLNSRMETSIGEDEQGTLILLDIDDFKKVN 505 Query: 400 DTYGHQKGDKILRQVSEIVKSSIRQGDIAARWGGEELAVYLPQLGVQQAVFVAERIRKQV 459 DTYGHQ GD+IL QV++++K +IR DI ARWGGEE+A+YLP + +Q V VA+R+ V Sbjct: 506 DTYGHQVGDEILIQVAKLIKENIRSTDIGARWGGEEMAIYLPGVPLQTGVQVAKRVLAAV 565 Query: 460 MSETDPRVTVSCGIAEWSWTDDKVSVESLFYRADMALYEAKNNGRNQVVL 509 T+P VT+SCG++ W+ ++ +VESLF RAD LY AK+ G+NQVV+ Sbjct: 566 EKNTNPPVTISCGVSYWNKEMNE-TVESLFNRADAGLYHAKSLGKNQVVI 614 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,889,679,023 Number of extensions: 115294283 Number of successful extensions: 288807 Number of sequences better than 1.0e-05: 13848 Number of HSP's gapped: 272285 Number of HSP's successfully gapped: 13880 Length of query: 521 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 381 Effective length of database: 1,741,754,103 Effective search space: 663608313243 Effective search space used: 663608313243 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)