BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0010.p (272 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DHP4.1 B1DHP4_9BACL Calcium/proton antiporter, CaCA family 275 3e-72 C0ZBS2.1 C0ZBS2_BREBN Conserved hypothetical membrane protein 222 3e-56 Q8XKM0.1 Q8XKM0_CLOPE Probable H+/Ca2+ exchanging protein 205 3e-51 B1RSE1.1 B1RSE1_CLOPE Calcium/proton exchanger 205 3e-51 B1BXN7.1 B1BXN7_CLOPE Calcium/proton exchanger 205 3e-51 >B1DHP4.1 B1DHP4_9BACL Calcium/proton antiporter, CaCA family Length = 352 Score = 275 bits (703), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 161/237 (67%), Positives = 187/237 (78%), Gaps = 4/237 (1%) Query: 13 LVITFILSAIGHYADWDHTLQFVLSAISVVFVAGFLGRATESVAHYAGQRLGGFLNATFG 72 L++ F+LSAIGH+A WD TLQF++SAI+VVFVAGFLG+ATESVAHYAGQRLGGFLNATFG Sbjct: 10 LIVMFLLSAIGHFAHWDMTLQFIVSAIAVVFVAGFLGKATESVAHYAGQRLGGFLNATFG 69 Query: 73 NAAELIIAFFLVKEGLFDMVKASLTGSIIGNLLLVLGLSIFAGGMKFKVQNFNVTLAGLN 132 NAAELIIA FLVKEGLFD VKAS+TGSIIGNLLLVLGLS+ AGG+KFK Q FN LA N Sbjct: 70 NAAELIIAIFLVKEGLFDTVKASITGSIIGNLLLVLGLSLLAGGIKFKQQFFNNQLAEHN 129 Query: 133 GSLMIVAVIALFVPAMFFNTHSITEKDTDVLSLVVAGLLIAAYMAWLIFSMITHKKYLAD 192 +LMI+AVI LFVPA+F T LS+ VA +LI +Y+ WL+FSMITHK D Sbjct: 130 ATLMIMAVIGLFVPAVFVGAEHFTLNKDHFLSITVACILIVSYLLWLVFSMITHK----D 185 Query: 193 VTVDNEEELPNEHKPAWSMGRSVIYLILATVMVAFVSEWLVGTLETLTERFGFSELF 249 V DN + +P WS G SVI LI+ATVMVAFVSEWLV TL++ TE+FG S+LF Sbjct: 186 VLADNVMNADHGEEPMWSKGVSVIMLIVATVMVAFVSEWLVHTLDSFTEKFGLSQLF 242 >C0ZBS2.1 C0ZBS2_BREBN Conserved hypothetical membrane protein Length = 356 Score = 222 bits (565), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 126/231 (54%), Positives = 169/231 (73%), Gaps = 9/231 (3%) Query: 19 LSAIGHYADWDHTLQFVLSAISVVFVAGFLGRATESVAHYAGQRLGGFLNATFGNAAELI 78 L+ HY + LQFV ++++++F+A +LG++TESVAHYAG R+GGFLNATFGNAAELI Sbjct: 25 LAVYAHYFSGNEMLQFVTASLAIIFLAAWLGKSTESVAHYAGDRIGGFLNATFGNAAELI 84 Query: 79 IAFFLVKEGLFDMVKASLTGSIIGNLLLVLGLSIFAGGMKFKVQNFNVTLAGLNGSLMIV 138 IAFFLVKEGLFDMVKAS+TG+IIGN+LLVLGLS GG+K+K Q FN LA N SLM + Sbjct: 85 IAFFLVKEGLFDMVKASITGAIIGNMLLVLGLSTLLGGLKYKEQTFNSRLASHNASLMTL 144 Query: 139 AVIALFVPAMFFNTHSITEKDTDVLSLVVAGLLIAAYMAWLIFSMITHKKYLADVTVDNE 198 A++ALF+PA+F + + +++S+V+A LLI AY+ WLIFSMITH +L+D+ Sbjct: 145 AIVALFIPAVFMFDLPVIK--VEIISIVIASLLIIAYILWLIFSMITHSDFLSDIE---- 198 Query: 199 EELPNEHKPAWSMGRSVIYLILATVMVAFVSEWLVGTLETLTERFGFSELF 249 P E W G S++ L ++T VA VSEWLV ++ ++E G+SELF Sbjct: 199 ---PQESDAVWPKGVSLLMLAVSTFFVAVVSEWLVEGVQHVSESLGWSELF 246 >Q8XKM0.1 Q8XKM0_CLOPE Probable H+/Ca2+ exchanging protein Length = 348 Score = 205 bits (522), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 115/246 (46%), Positives = 169/246 (68%), Gaps = 13/246 (5%) Query: 6 KWISPALLVITFILSAIGHYADWDHTLQFVLSAISVVFVAGFLGRATESVAHYAGQRLGG 65 KW L+I +S IG + + TL FVLSA+++V +AG +G ATE ++ Y G +LGG Sbjct: 5 KW-----LLIFIPISIIGEFMHFSPTLMFVLSALAIVPLAGLMGEATEEISFYTGPKLGG 59 Query: 66 FLNATFGNAAELIIAFFLVKEGLFDMVKASLTGSIIGNLLLVLGLSIFAGGMKFKVQNFN 125 FLNATFGNA ELII+FF +K+GLFD+VKAS+ GS+IGN+LLVLG S+ AGG+K+K Q+FN Sbjct: 60 FLNATFGNATELIISFFALKDGLFDVVKASIAGSVIGNILLVLGASMLAGGLKYKTQSFN 119 Query: 126 VTLAGLNGSLMIVAVIALFVPAMFFNTHSITEKDT--DVLSLVVAGLLIAAYMAWLIFSM 183 + + S+++ AVI L +PA+F +T +T + LS++VA ++ A Y+ LIFS Sbjct: 120 KKVMETSSSMLLFAVIGLTIPAIFMHTIKPEFLNTKYESLSVIVAVVMFAIYILSLIFSF 179 Query: 184 ITHKKYLADVTVDNEEELPNEHKPAWSMGRSVIYLILATVMVAFVSEWLVGTLETLTERF 243 THK A V+ +EE + + WS+ +++ L++ATV++A SE+LVG +E +TE Sbjct: 180 FTHKDIYA---VEQDEE---DGEAKWSLKKAITVLVVATVIIAIESEFLVGAVEPMTETL 233 Query: 244 GFSELF 249 G S+LF Sbjct: 234 GLSKLF 239 >B1RSE1.1 B1RSE1_CLOPE Calcium/proton exchanger Length = 348 Score = 205 bits (522), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 115/246 (46%), Positives = 169/246 (68%), Gaps = 13/246 (5%) Query: 6 KWISPALLVITFILSAIGHYADWDHTLQFVLSAISVVFVAGFLGRATESVAHYAGQRLGG 65 KW L+I +S IG + + TL FVLSA+++V +AG +G ATE ++ Y G +LGG Sbjct: 5 KW-----LLIFIPISIIGEFMHFSPTLMFVLSALAIVPLAGLMGEATEEISFYTGPKLGG 59 Query: 66 FLNATFGNAAELIIAFFLVKEGLFDMVKASLTGSIIGNLLLVLGLSIFAGGMKFKVQNFN 125 FLNATFGNA ELII+FF +K+GLFD+VKAS+ GS+IGN+LLVLG S+ AGG+K+K Q+FN Sbjct: 60 FLNATFGNATELIISFFALKDGLFDVVKASIAGSVIGNILLVLGASMLAGGLKYKTQSFN 119 Query: 126 VTLAGLNGSLMIVAVIALFVPAMFFNTHSITEKDT--DVLSLVVAGLLIAAYMAWLIFSM 183 + + S+++ AVI L +PA+F +T +T + LS++VA ++ A Y+ LIFS Sbjct: 120 KKVMETSSSMLLFAVIGLTIPAIFMHTIKPEFLNTKYESLSVIVAVVMFAIYILSLIFSF 179 Query: 184 ITHKKYLADVTVDNEEELPNEHKPAWSMGRSVIYLILATVMVAFVSEWLVGTLETLTERF 243 THK A V+ +EE + + WS+ +++ L++ATV++A SE+LVG +E +TE Sbjct: 180 FTHKDIYA---VEQDEE---DGEAKWSLKKAITVLVVATVIIAIESEFLVGAVEPMTETL 233 Query: 244 GFSELF 249 G S+LF Sbjct: 234 GLSKLF 239 >B1BXN7.1 B1BXN7_CLOPE Calcium/proton exchanger Length = 348 Score = 205 bits (522), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 115/246 (46%), Positives = 169/246 (68%), Gaps = 13/246 (5%) Query: 6 KWISPALLVITFILSAIGHYADWDHTLQFVLSAISVVFVAGFLGRATESVAHYAGQRLGG 65 KW L+I +S IG + + TL FVLSA+++V +AG +G ATE ++ Y G +LGG Sbjct: 5 KW-----LLIFIPISIIGEFMHFSPTLMFVLSALAIVPLAGLMGEATEEISFYTGPKLGG 59 Query: 66 FLNATFGNAAELIIAFFLVKEGLFDMVKASLTGSIIGNLLLVLGLSIFAGGMKFKVQNFN 125 FLNATFGNA ELII+FF +K+GLFD+VKAS+ GS+IGN+LLVLG S+ AGG+K+K Q+FN Sbjct: 60 FLNATFGNATELIISFFALKDGLFDVVKASIAGSVIGNILLVLGASMLAGGLKYKTQSFN 119 Query: 126 VTLAGLNGSLMIVAVIALFVPAMFFNTHSITEKDT--DVLSLVVAGLLIAAYMAWLIFSM 183 + + S+++ AVI L +PA+F +T +T + LS++VA ++ A Y+ LIFS Sbjct: 120 KKVMETSSSMLLFAVIGLTIPAIFMHTIKPEFLNTKYESLSVIVAIVMFAIYILSLIFSF 179 Query: 184 ITHKKYLADVTVDNEEELPNEHKPAWSMGRSVIYLILATVMVAFVSEWLVGTLETLTERF 243 THK A V+ +EE + + WS+ +++ L++ATV++A SE+LVG +E +TE Sbjct: 180 FTHKDIYA---VEQDEE---DGEAKWSLKKAITVLVVATVIIAIESEFLVGAVEPMTETL 233 Query: 244 GFSELF 249 G S+LF Sbjct: 234 GLSKLF 239 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.326 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,523,656,088 Number of extensions: 57755820 Number of successful extensions: 277349 Number of sequences better than 1.0e-05: 524 Number of HSP's gapped: 276864 Number of HSP's successfully gapped: 524 Length of query: 272 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 139 Effective length of database: 1,807,701,208 Effective search space: 251270467912 Effective search space used: 251270467912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)