BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0547.0007.p (393 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KJ12.1 B3KJ12_9BACI Amidohydrolase EC=3.5.1.14 523 e-147 B1DHP0.1 B1DHP0_9BACL Amidohydrolase EC=3.5.1.14 424 e-117 A4IQN1.1 A4IQN1_GEOTN N-acyl-L-amino acid amidohydrolase-like pr... 357 1e-96 B4BLE5.1 B4BLE5_9BACI Amidohydrolase EC=3.5.1.14 356 2e-96 Q5WE72.1 Q5WE72_BACSK N-acyl-L-amino acid amidohydrolase EC=3.5.... 352 5e-95 >B3KJ12.1 B3KJ12_9BACI Amidohydrolase EC=3.5.1.14 Length = 389 Score = 523 bits (1348), Expect = e-147, Method: Compositional matrix adjust. Identities = 248/388 (63%), Positives = 295/388 (76%), Gaps = 1/388 (0%) Query: 5 MDRLTLEQLLPDMVKWRRHLHRHPELSYQEKETSAFVAAKLIEFGIEVVRSKAGYGLTGI 64 M +Q P MV+WRRHLH HPELS+QEKETSAF+AA+L E G+ V G+G+ G Sbjct: 1 MKEAFWDQWYPRMVEWRRHLHMHPELSFQEKETSAFIAARLQELGLAVKTGVGGHGVIGT 60 Query: 65 LKGKSPGKTVVLRADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXX 124 LKG PG+TVVLR+DMDAL I + EY S+ GVMHACGHDGH SM Sbjct: 61 LKGDKPGRTVVLRSDMDALPIEDGKSCEYKSRVQGVMHACGHDGHASMLLGAAAYYSTFP 120 Query: 125 XXLQGELRFLFQPAEEICPGGALGMIAEGVLEGADAVYGLHLWTPLPVGKVASAPGPLMA 184 +QGE+RF+FQPAEE+CPGGA+ MI +G LEGAD VYGLHLWTPLPVG ASAPGPLMA Sbjct: 121 EEIQGEIRFMFQPAEEVCPGGAVEMIKDGALEGADVVYGLHLWTPLPVGTAASAPGPLMA 180 Query: 185 SADEFFIDITGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQGG 244 +ADEFFIDITG+GGH G PH T D++VA AALV QLQ+IVSR+VDP++P+VV+VGT+Q G Sbjct: 181 AADEFFIDITGRGGHGGMPHVTADALVAGAALVMQLQTIVSRTVDPLQPSVVTVGTMQAG 240 Query: 245 TAQNIVAERCRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGYPPLVND 304 TAQN++A CRITGTVR FDE TR LIR+RIE MT V+ +YG +A + YL+GYPP+VND Sbjct: 241 TAQNVIASSCRITGTVRTFDEPTRALIRERIEHMTRTVSETYGTKAAVRYLVGYPPVVND 300 Query: 305 EAEFQRYFRVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYPH 364 EAE R+FR AP + V K+MPAEDF+YY+KEIPGCFIFVGAGNPDK A+YPH Sbjct: 301 EAETARFFRTAPKVFDAD-QVIVSPKLMPAEDFAYYLKEIPGCFIFVGAGNPDKGAIYPH 359 Query: 365 HHSKFDFDEDALLHGAKVLVAMADSCMN 392 HH FDFDEDA+ +GAK+LV M S N Sbjct: 360 HHPMFDFDEDAMRYGAKLLVEMVSSYQN 387 >B1DHP0.1 B1DHP0_9BACL Amidohydrolase EC=3.5.1.14 Length = 393 Score = 424 bits (1091), Expect = e-117, Method: Compositional matrix adjust. Identities = 196/379 (51%), Positives = 267/379 (70%), Gaps = 1/379 (0%) Query: 10 LEQLLPDMVKWRRHLHRHPELSYQEKETSAFVAAKLIEFGIEVVRSKAGYGLTGILKGKS 69 L++L P+MV+WRRHLH++PELS+QE TS ++A +L FG EV G+GL +KG+ Sbjct: 11 LQRLYPEMVRWRRHLHQNPELSFQEVHTSRWIAEQLKAFGCEVREGVGGHGLLVTIKGEK 70 Query: 70 PGKTVVLRADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXXXXLQG 129 PG + LRAD+DAL I +E EY S+ GVMHACGHDGHTS L G Sbjct: 71 PGPVIALRADIDALPIQDEKECEYRSKVPGVMHACGHDGHTSALLAVASFYQQHKAELAG 130 Query: 130 ELRFLFQPAEEICPGGALGMIAEGVLEGADAVYGLHLWTPLPVGKVASAPGPLMASADEF 189 E R +FQPAEE PGGA+ MI +G L+G DA+YG+HLW+P+P G+ A+ PGP MA+ADEF Sbjct: 131 ERRLIFQPAEEQTPGGAIRMIEDGALDGVDAIYGVHLWSPIPYGQTATKPGPFMAAADEF 190 Query: 190 FIDITGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQGGTAQNI 249 ++I G GGH G PH+TVD+IV +ALV +QSIV R+VDP++PAVV++G+ Q GT N+ Sbjct: 191 TLEIIGLGGHGGMPHKTVDTIVIGSALVQAVQSIVGRNVDPLKPAVVTIGSFQAGTTNNV 250 Query: 250 VAERCRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGYPPLVNDEAEFQ 309 +AERC + GTVR FDEE+R L R+E++ + YGA+ +GYPP++NDE E Sbjct: 251 IAERCAMKGTVRTFDEESRKLTHDRLEQLINHTCSMYGAKYDYHMRIGYPPVINDEQEAD 310 Query: 310 RYFRVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYPHHHSKF 369 R+FRV+ G E ++ R E +M AEDFSYY++++PGCF+FVGAGN + V+ HHH +F Sbjct: 311 RFFRVSGELFGTENTL-RSEAMMVAEDFSYYLEKVPGCFMFVGAGNEETGVVFAHHHPRF 369 Query: 370 DFDEDALLHGAKVLVAMAD 388 D DE A++H A++L+ MA+ Sbjct: 370 DIDERAMVHSAQLLIQMAE 388 >A4IQN1.1 A4IQN1_GEOTN N-acyl-L-amino acid amidohydrolase-like protein Length = 386 Score = 357 bits (915), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 169/372 (45%), Positives = 234/372 (62%), Gaps = 2/372 (0%) Query: 5 MDRLTLEQLLPDMVKWRRHLHRHPELSYQEKETSAFVAAKLIEFGIEVVRSKAGYGLTGI 64 M + + + + +++KWRR+ H++PELS++EK TS V L G+ V + GYG+ Sbjct: 1 MIKNSHQTISTEVIKWRRYFHQYPELSFEEKRTSKVVGEFLKSIGLHVKENVNGYGVVAD 60 Query: 65 LKGKSPGKTVVLRADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXX 124 L G G T+ RADMDAL I EE G +AS+ GVMHACGHDGHT++ Sbjct: 61 LIGSEKGPTIAFRADMDALPIQEETGLPFASKIPGVMHACGHDGHTAILMGAAALLAAQK 120 Query: 125 XXLQGELRFLFQPAEEICPGGALGMIAEGVLEGADAVYGLHLWTPLPVGKVASAPGPLMA 184 L+G +RF+FQPAEE+ PGGA+GMI EGVL G DA++GLHLW+ P G + GP+M+ Sbjct: 121 NKLKGNVRFIFQPAEELSPGGAIGMIREGVLHGVDAIFGLHLWSEFPSGTFWTCYGPMMS 180 Query: 185 SADEFFIDITGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQGG 244 S D F I+I GKGGH G PH+ +DSIV A+ L+ Q I+SR++DP+ V++ G + G Sbjct: 181 STDHFMIEIEGKGGHGGMPHKAIDSIVIASHLIMSAQHIISRNIDPLESGVITFGKLHAG 240 Query: 245 TAQNIVAERCRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGYPPLVND 304 TA NI+A + GTVR+F E R ++ R+EE+ + YGA+ ++Y GYP ++N Sbjct: 241 TAFNIIANNALLEGTVRSFTPEVRKTLQTRLEELIEGLEKIYGAKITMNYRQGYPSVINH 300 Query: 305 EAEFQRYFRVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYPH 364 + E + VA G E + M +M EDFSYY+KEIPG F FVGAG+P+ +YPH Sbjct: 301 DKEVEMVIGVAKEVFGVE-NTRIMRPVMVGEDFSYYLKEIPGAFCFVGAGDPN-HPIYPH 358 Query: 365 HHSKFDFDEDAL 376 HH +F DE L Sbjct: 359 HHPRFQIDESVL 370 >B4BLE5.1 B4BLE5_9BACI Amidohydrolase EC=3.5.1.14 Length = 386 Score = 356 bits (913), Expect = 2e-96, Method: Compositional matrix adjust. Identities = 169/372 (45%), Positives = 234/372 (62%), Gaps = 2/372 (0%) Query: 5 MDRLTLEQLLPDMVKWRRHLHRHPELSYQEKETSAFVAAKLIEFGIEVVRSKAGYGLTGI 64 M + + + + +++KWRR+ H++PELS++EK TS V L G+ V + GYG+ Sbjct: 1 MIKNSHQTISTEVIKWRRYFHQYPELSFEEKRTSKVVGEFLKSIGLHVKENVNGYGVVAD 60 Query: 65 LKGKSPGKTVVLRADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXX 124 L G G T+ RADMDAL I EE G +AS+ GVMHACGHDGHT++ Sbjct: 61 LIGSEKGPTIAFRADMDALPIQEETGLPFASKIPGVMHACGHDGHTAILMGAAALLAAQK 120 Query: 125 XXLQGELRFLFQPAEEICPGGALGMIAEGVLEGADAVYGLHLWTPLPVGKVASAPGPLMA 184 L+G +RF+FQPAEE+ PGGA+GMI EGVL G DA++GLHLW+ P G + GP+M+ Sbjct: 121 NKLKGNVRFIFQPAEELSPGGAIGMIREGVLHGVDAIFGLHLWSEFPSGTFWTCYGPMMS 180 Query: 185 SADEFFIDITGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQGG 244 S D F I+I GKGGH G PH+ +DSIV A+ L+ Q I+SR++DP+ V++ G + G Sbjct: 181 STDHFMIEIEGKGGHGGMPHKAIDSIVIASHLIMSAQHIISRNIDPLESGVITFGKLHAG 240 Query: 245 TAQNIVAERCRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGYPPLVND 304 TA NI+A + GTVR+F E R ++ R+EE+ + YGA+ ++Y GYP ++N Sbjct: 241 TAFNIIANTALLEGTVRSFTPEVRKTLQTRLEELIEGLEKIYGAKITMNYRQGYPAVINH 300 Query: 305 EAEFQRYFRVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYPH 364 + E + VA G E + M +M EDFSYY+KEIPG F FVGAG+P+ +YPH Sbjct: 301 DKEVEMVIGVAKEVFGVE-NTRIMRPVMVGEDFSYYLKEIPGAFCFVGAGDPN-HPIYPH 358 Query: 365 HHSKFDFDEDAL 376 HH +F DE L Sbjct: 359 HHPRFQIDESVL 370 >Q5WE72.1 Q5WE72_BACSK N-acyl-L-amino acid amidohydrolase EC=3.5.1.14 Length = 400 Score = 352 bits (902), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 179/383 (46%), Positives = 235/383 (61%), Gaps = 2/383 (0%) Query: 10 LEQLLPDMVKWRRHLHRHPELSYQEKETSAFVAAKLIEFGIEVVRSK-AGYGLTGILKGK 68 LE L +++WRRHLH +PELS++E ET AF+ KL E G +R G G+ L GK Sbjct: 16 LETLQAQVIEWRRHLHANPELSFEEVETPAFIVQKLKEIGFTDIREHVGGRGVVAKLHGK 75 Query: 69 SPGKTVVLRADMDALAITEENGREYASQNHGVMHACGHDGHTSMXXXXXXXXXXXXXXLQ 128 PG T+ RAD DAL I EEN YAS GVMHACGHDGHT+ L+ Sbjct: 76 KPGPTIAFRADFDALPIHEENDVSYASTKPGVMHACGHDGHTAALLGVAATLFDQVDELR 135 Query: 129 GELRFLFQPAEEICPGGALGMIAEGVLEGADAVYGLHLWTPLPVGKVASAPGPLMASADE 188 G + FLFQ AEE PGGA MIA+G LEG DAV+G H+ + +P+G++ ++PG +MA+ D Sbjct: 136 GTIVFLFQHAEEKPPGGAREMIADGCLEGVDAVFGAHVSSQIPLGQINASPGAVMAAVDA 195 Query: 189 FFIDITGKGGHAGTPHRTVDSIVAAAALVTQLQSIVSRSVDPMRPAVVSVGTIQGGTAQN 248 F + I GKGGH PH T+DSIV + LV LQ+IVSR ++PM AVV+VG Q GTA N Sbjct: 196 FTVHIQGKGGHGAHPHSTIDSIVIGSQLVNDLQTIVSRRINPMDTAVVTVGVFQAGTAFN 255 Query: 249 IVAERCRITGTVRAFDEETRYLIRQRIEEMTAAVAASYGAEAKIDYLMGYPPLVNDEAEF 308 ++A+ RI GTVR F EETR I + I + + IDYL GYPPLVN E E Sbjct: 256 VIADTARIEGTVRTFQEETRAFIEEEIRAIVSGKEHGGHVTCTIDYLNGYPPLVNAEKET 315 Query: 309 QRYFRVAPAALGREVSVERMEKIMPAEDFSYYVKEIPGCFIFVGAGNPDKEAVYPHHHSK 368 + +A G E +V + + EDF+YY++E PGCF VG ++ +PHHH + Sbjct: 316 EVIRDLAKGVFGEE-NVLMLPAALGGEDFAYYLEEKPGCFFHVGGRTEEERTQFPHHHPR 374 Query: 369 FDFDEDALLHGAKVLVAMADSCM 391 FDFDE AL H ++ +A+A+ + Sbjct: 375 FDFDERALFHIGEMFLAIANQYL 397 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,293,920,655 Number of extensions: 92632521 Number of successful extensions: 242966 Number of sequences better than 1.0e-05: 2885 Number of HSP's gapped: 234793 Number of HSP's successfully gapped: 2889 Length of query: 393 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 255 Effective length of database: 1,760,596,133 Effective search space: 448952013915 Effective search space used: 448952013915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)