BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0068.p (232 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1D5V2.1 B1D5V2_9BACL Short-chain dehydrogenase/reductase SDR 286 9e-76 B5YA82.1 B5YA82_DICT6 Oxidoreductase, short chain dehydrogenase/... 149 2e-34 B8E344.1 B8E344_DICTD Short-chain dehydrogenase/reductase SDR 136 2e-30 B8I9D4.1 B8I9D4_CLOCE Short-chain dehydrogenase/reductase SDR 130 1e-28 Q4H4F1.1 Q4H4F1_BACCI Putative oxidoreductase 126 1e-27 >B1D5V2.1 B1D5V2_9BACL Short-chain dehydrogenase/reductase SDR Length = 231 Score = 286 bits (732), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 137/232 (59%), Positives = 174/232 (75%), Gaps = 1/232 (0%) Query: 1 MNILITXXXXXXXXXXXXXXXXXXHGVIAGIRHPGQEPEALAQLAAIHGAKLTIAALDVT 60 MNILIT H V+AG+R + + LA+L +G +L + LDVT Sbjct: 1 MNILITGSNRGLGYEIAAEALERGHHVLAGVRDTAAQGK-LAELVKQYGGQLELVELDVT 59 Query: 61 DEAGIASLAAALKGQGRTLGAIINNAAVLTARDTPIEALDLQDMLTTMDINLYGPMRVVK 120 DEAG+A+LA L+ QG+ +GAI+NNAA+L R+TPIE L+++++ T DINL+GPMRV K Sbjct: 60 DEAGVAALAGKLQQQGKAIGAIVNNAAILVERNTPIEELNIEEVAKTFDINLFGPMRVAK 119 Query: 121 HFLPLLTEPEGSIINISSEAGSISNAYPGDYPYALSKTALNMFSQQLHVYLQERGIQVFS 180 HFLPL+TE +G+IINISSEAGSISNAYPGDYPY +SKTALNMF+QQL ++ERGIQV S Sbjct: 120 HFLPLVTEKQGTIINISSEAGSISNAYPGDYPYGISKTALNMFTQQLLSAVKERGIQVLS 179 Query: 181 VHPGWMHTDMGGAEAPTDPRRSAGGIISLMEQRIAPQGRFRFVDYTGKDMDI 232 VHPGWM TDMGG APTDPR+SA GI+ ++E ++ PQG+F FVDYTGK+M I Sbjct: 180 VHPGWMKTDMGGENAPTDPRQSARGILDMIEGQLVPQGKFNFVDYTGKEMPI 231 >B5YA82.1 B5YA82_DICT6 Oxidoreductase, short chain dehydrogenase/reductase family Length = 233 Score = 149 bits (376), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 83/209 (39%), Positives = 123/209 (58%), Gaps = 8/209 (3%) Query: 25 HGVIAGIRHPGQEPEALAQLAAIHGAKLTIAALDVTDEAGIASLAAALKGQGRTLGAIIN 84 H VIA R ++ E + + L + +DV+DE + + LK + + +IN Sbjct: 28 HKVIATYRKNLEKLEEIK-----NHPNLILHPMDVSDEESVRNTYEYLKNKINYIDILIN 82 Query: 85 NAAV-LTARDTPIEALDLQDMLTTMDINLYGPMRVVKHFLPLLTEPEGS-IINISSEAGS 142 NAAV L + IE +D++ + TM++N GP+RV+K+F L+ + IINISSEAGS Sbjct: 83 NAAVYLEDKSKTIENIDIEKAIVTMNVNSIGPLRVLKYFYSLVEKGNKKLIINISSEAGS 142 Query: 143 ISNAYPG-DYPYALSKTALNMFSQQLHVYLQERGIQVFSVHPGWMHTDMGGAEAPTDPRR 201 ISN + +Y Y +SK+ALNM S L Y +GI+V S+HPGWM TDMGG +A DP+ Sbjct: 143 ISNCWRDREYAYCMSKSALNMLSAILQNYSIPKGIKVLSIHPGWMRTDMGGPDADIDPKE 202 Query: 202 SAGGIISLMEQRIAPQGRFRFVDYTGKDM 230 +A GI +L + P+ + ++DY G M Sbjct: 203 AAEGIYNLSVKDWDPKDKNIYMDYKGNLM 231 >B8E344.1 B8E344_DICTD Short-chain dehydrogenase/reductase SDR Length = 233 Score = 136 bits (342), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 79/209 (37%), Positives = 118/209 (56%), Gaps = 8/209 (3%) Query: 25 HGVIAGIRHPGQEPEALAQLAAIHGAKLTIAALDVTDEAGIASLAAALKGQGRTLGAIIN 84 H VIA R ++ E + + L + +DV+DE + + LK + + +IN Sbjct: 28 HKVIATYRKNLEKLEEIK-----NHPNLILYTMDVSDENSVKNAFDFLKDKISHIDILIN 82 Query: 85 NAAV-LTARDTPIEALDLQDMLTTMDINLYGPMRVVKHFLPLLTEPEGS-IINISSEAGS 142 NAAV L + IE +D+++ +TT++IN GP+RV+K+F + IIN+SSEAGS Sbjct: 83 NAAVYLEDKTKTIENVDIEEAITTLNINSIGPLRVLKYFYSFVERGNKKLIINVSSEAGS 142 Query: 143 ISNAYPG-DYPYALSKTALNMFSQQLHVYLQERGIQVFSVHPGWMHTDMGGAEAPTDPRR 201 ISN + +Y Y +SK+ALNM S L Y +GI+V ++HPGWM TDMGG A P Sbjct: 143 ISNCWRDREYAYCMSKSALNMLSAILQNYSIPKGIKVLAIHPGWMRTDMGGPNADIAPEE 202 Query: 202 SAGGIISLMEQRIAPQGRFRFVDYTGKDM 230 +A GI +L + + ++DY G M Sbjct: 203 AAEGIYNLSMKDWDISDKNIYMDYKGNLM 231 >B8I9D4.1 B8I9D4_CLOCE Short-chain dehydrogenase/reductase SDR Length = 234 Score = 130 bits (326), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 77/193 (39%), Positives = 105/193 (54%), Gaps = 4/193 (2%) Query: 39 EALAQLAAIHGAKLTIAALDVTDEAGIASLAAALKGQGRTLGAIINNAAVLTARDTPI-E 97 E ++ L + + DV+ ++ + A +K TL +IN AA+L I E Sbjct: 37 EKISVLETDYPNQFKFFNTDVSLDSAVEETAKKIKQNSDTLDILINAAAILPTNSANILE 96 Query: 98 ALDLQDMLTTMDINLYGPMRVVKHFLPLLTEPEGS-IINISSEAGSISN--AYPGDYPYA 154 ++ L N GP+RVVK LPL+ + E IINISSEAGS++ Y Y Y Sbjct: 97 DFEISQSLEVFSTNSLGPLRVVKSLLPLIRKGEDKLIINISSEAGSLATHCNYVNRYDYC 156 Query: 155 LSKTALNMFSQQLHVYLQERGIQVFSVHPGWMHTDMGGAEAPTDPRRSAGGIISLMEQRI 214 +SK ALNM S L YL+ GI+V +VHPGW+ T+MGG +AP P SA GI++L E+ I Sbjct: 157 MSKAALNMQSVILQRYLKPDGIKVLAVHPGWVRTEMGGLDAPILPSESAAGIVTLAEKYI 216 Query: 215 APQGRFRFVDYTG 227 G F DY G Sbjct: 217 HKTGEGIFFDYDG 229 >Q4H4F1.1 Q4H4F1_BACCI Putative oxidoreductase Length = 232 Score = 126 bits (317), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 74/207 (35%), Positives = 110/207 (53%), Gaps = 2/207 (0%) Query: 25 HGVIAGIRHPGQEPEALAQLAAIHGAKLTIAALDVTDEAGIASLAAALKGQGRTLGAIIN 84 H V+AG+R+ + + L L + +L+I +DV+ + I + + L AIIN Sbjct: 25 HQVLAGVRNMNRAGQLLP-LKTKYKEELSIIPVDVSSQDSIRQAYSLITAVTPKLDAIIN 83 Query: 85 NAAVLTARDTPIEALDLQDMLTTMDINLYGPMRVVKHFLPLLTEPE-GSIINISSEAGSI 143 NAA R I+ +D+ D T N+ GPM ++K P++ SI+NISSEAGSI Sbjct: 84 NAAREGIRCKKIQEVDIADFEETWKTNVLGPMAIIKSLKPMMESSSVKSILNISSEAGSI 143 Query: 144 SNAYPGDYPYALSKTALNMFSQQLHVYLQERGIQVFSVHPGWMHTDMGGAEAPTDPRRSA 203 S Y Y +SK ALNMF++ L L+ +V +VHPGWM T+MGG +AP +P A Sbjct: 144 SELPLKTYGYCISKAALNMFTRMLERELKPENFKVVAVHPGWMKTEMGGEDAPYEPGEIA 203 Query: 204 GGIISLMEQRIAPQGRFRFVDYTGKDM 230 + + + + F VD+ G M Sbjct: 204 RHLYERLTESPGKKADFNIVDHLGNRM 230 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,252,321,773 Number of extensions: 50231604 Number of successful extensions: 182895 Number of sequences better than 1.0e-05: 6031 Number of HSP's gapped: 188843 Number of HSP's successfully gapped: 6033 Length of query: 232 Length of database: 3,060,696,203 Length adjustment: 131 Effective length of query: 101 Effective length of database: 1,826,543,238 Effective search space: 184480867038 Effective search space used: 184480867038 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)