BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0066.p (472 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KIT2.1 B3KIT2_9BACI Amino acid permease-associated region 467 e-129 Q9KAW5.1 Q9KAW5_BACHD Aromatic amino acid transporter 429 e-118 Q2BDP9.1 Q2BDP9_9BACI Proline-specific permease 410 e-112 B7HWM0.1 B7HWM0_BACC7 Amino acid permease family protein 399 e-109 B5V262.1 B5V262_BACCE Amino acid permease family protein 399 e-109 >B3KIT2.1 B3KIT2_9BACI Amino acid permease-associated region Length = 462 Score = 467 bits (1202), Expect = e-129, Method: Compositional matrix adjust. Identities = 240/422 (56%), Positives = 306/422 (72%), Gaps = 2/422 (0%) Query: 24 VAGTIGTGYFLGSSLAISIGGPAVLLVYILAAAGTYVVFDALSRMTADHPEQGSFRSYAK 83 VA TIGTGYFLGS+L I +GGPA LL ILAA TY VFD L+RMTA+ P++GSFR+YAK Sbjct: 26 VASTIGTGYFLGSALGIRLGGPAFLLSIILAATATYFVFDVLARMTAEDPQKGSFRTYAK 85 Query: 84 KAFGSWAGFASGWVYWFSELLIMGSQLTALAIFSRFWFPAVPMWXXXXXXXXXXXXXXXX 143 KA+G WAGF++GW+YW SE+LIMGSQ+TALAIFSRFWFP VPMW Sbjct: 86 KAYGRWAGFSTGWMYWCSEVLIMGSQMTALAIFSRFWFPGVPMWIFASGYAVLGLFVIWI 145 Query: 144 XNKGFDRVENVLAVIKXXXXXXXXXXXXXXXXXXXXXXKYTPKLPLTAKALFPAGGIGLW 203 KGFDR+ENVLAVIK P +P + ALFP GG G+W Sbjct: 146 GTKGFDRLENVLAVIKVAAIVMFLVIAAAAWMGWLKGGT-KPHVPGSMNALFPTGGKGVW 204 Query: 204 SAFLFAFYAYGGIEVLGIMSYRLNKPEEAPKAGKVMLLTLTAVYVISIGLAVTMVPLSAF 263 S+ +FAFYA+GGIEV+G+M+ RL P+EAPK+GKVM+L L+ VYV+SIG+AV MV + F Sbjct: 205 SSLIFAFYAFGGIEVMGVMAVRLKNPKEAPKSGKVMILLLSIVYVLSIGMAVMMVAWNRF 264 Query: 264 NPKESPFVLALSSDHLAFVPHLFNGVLIIAGFSTMTASLYAVTSMMLTLAQEGDAPRLFS 323 + +SPFV+AL+S L F+PHLFN VLIIAGFSTM ASLYAVT+M++TL+++ DAP+LFS Sbjct: 265 STTKSPFVVALNSYQLPFIPHLFNAVLIIAGFSTMVASLYAVTTMLVTLSEDKDAPKLFS 324 Query: 324 KKL-KGKYPLYALCLISGGLIAAIVMSLLLPGKVYEYITTAAGLMLLYNWSFILLSSGKL 382 +K+ K P+++L L + GLI +IV+SLL+PGK+YEY+TTAAGLMLLYNW FIL+S+G+L Sbjct: 325 RKMFKRDTPIFSLSLTAAGLIVSIVLSLLMPGKIYEYLTTAAGLMLLYNWFFILISAGRL 384 Query: 383 LEPVKWSGLKRWTGLVLIVLAVAGTLFHKLSRPGLYISLLLVALIVGTDLIVHKVRSKAG 442 +E W +KR+ G++LI AV+GT H SRPGL+ISL + LI L + V K+G Sbjct: 385 MELTGWGKVKRYIGMILIAAAVSGTALHPTSRPGLWISLGFILLIGLVVLCMRSVWKKSG 444 Query: 443 SN 444 Sbjct: 445 QG 446 >Q9KAW5.1 Q9KAW5_BACHD Aromatic amino acid transporter Length = 455 Score = 429 bits (1102), Expect = e-118, Method: Compositional matrix adjust. Identities = 225/437 (51%), Positives = 298/437 (68%), Gaps = 4/437 (0%) Query: 24 VAGTIGTGYFLGSSLAISIGGPAVLLVYILAAAGTYVVFDALSRMTADHPEQGSFRSYAK 83 V IGTG+FLGSS+AI + GP+VLL +ILA GTY+VFDAL++MT+ PE+GSFRSYAK Sbjct: 22 VGCIIGTGFFLGSSIAIQMAGPSVLLAFILAGIGTYIVFDALAQMTSADPEKGSFRSYAK 81 Query: 84 KAFGSWAGFASGWVYWFSELLIMGSQLTALAIFSRFWFPAVPMWXXXXXXXXXXXXXXXX 143 KA+G WAGF SGWVYW SE+LIMGSQLTAL+IFSRFWFP VP+W Sbjct: 82 KAYGRWAGFTSGWVYWASEMLIMGSQLTALSIFSRFWFPDVPLWIFASGFAVLGIIVILT 141 Query: 144 XNKGFDRVENVLAVIKXXXXXXXXXXXXXXXXXXXXXXKYTPKLPLTAKALFPAGGIGLW 203 +KGFDR+EN+ AV+K +P T LFP G +GLW Sbjct: 142 GSKGFDRIENLFAVMKVAAIVMFIALAILALFGVFRAGPEGAGIPRTWNGLFPEGFLGLW 201 Query: 204 SAFLFAFYAYGGIEVLGIMSYRLNKPEEAPKAGKVMLLTLTAVYVISIGLAVTMVPLSAF 263 A +F FYA+GGIE++G+++ RL + +A K+G+ MLL LT +YV+S+GLA+ +VPL+ F Sbjct: 202 PALIFGFYAFGGIEIMGLLANRLEEKGDAAKSGRAMLLLLTFLYVVSVGLALILVPLNRF 261 Query: 264 NPKESPFVLALSSDHLAFVPHLFNGVLIIAGFSTMTASLYAVTSMMLTLAQEGDAPRLFS 323 N + SPF+ AL L V HLFNGVLIIAGFSTM ASL+AVTS+++TLA + DAP LF+ Sbjct: 262 NTETSPFITALKPYGLPIVSHLFNGVLIIAGFSTMVASLFAVTSILVTLAADKDAPNLFA 321 Query: 324 KKLKGKYPLYALCLISGGLIAAIVMSLLLPGKVYEYITTAAGLMLLYNWSFILLSSGKLL 383 KK+K K L A+ L + G+ ++V+SLL+PG++YEY TTAAGLMLLYNW FI+ SSGKL Sbjct: 322 KKVKNKIALPAIGLTAIGMALSVVLSLLIPGRIYEYFTTAAGLMLLYNWLFIIASSGKL- 380 Query: 384 EPVKWSGLKRWTGLVLIVLAVAGTLFHKLSRPGLYISL-LLVALIVGTDLIVHKVRSKAG 442 +++ KRW G + I+LAV GTLFH+ SRPG SL LV + + T ++ + + + G Sbjct: 381 --IQYRLTKRWIGTLFILLAVIGTLFHRTSRPGFLFSLGFLVVIGIVTLMMKKRWKQEEG 438 Query: 443 SNGKTASAWLVPQNKSQ 459 K S ++ + S+ Sbjct: 439 DGKKRPSIFVKLKPNSE 455 >Q2BDP9.1 Q2BDP9_9BACI Proline-specific permease Length = 459 Score = 410 bits (1054), Expect = e-112, Method: Compositional matrix adjust. Identities = 214/400 (53%), Positives = 280/400 (70%), Gaps = 2/400 (0%) Query: 24 VAGTIGTGYFLGSSLAISIGGPAVLLVYILAAAGTYVVFDALSRMTADHPEQGSFRSYAK 83 V TIGTGYFL S++ I I GP+++ +ILAA GTY+VF+ L++MTA P++GSF YAK Sbjct: 33 VGCTIGTGYFLASAIGIKITGPSIVFSFILAALGTYIVFNLLAKMTAKDPQEGSFCYYAK 92 Query: 84 KAFGSWAGFASGWVYWFSELLIMGSQLTALAIFSRFWFPAVPMWXXXXXXXXXXXXXXXX 143 KA+G WAGF+ GW YW S +LIMGSQLTAL+I SRFWFP VP+W Sbjct: 93 KAYGKWAGFSCGWNYWTSNILIMGSQLTALSILSRFWFPHVPLWVFAAGYAILSILVVLT 152 Query: 144 XNKGFDRVENVLAVIKXXXXXXXXXXXXXXXXXXXXXXKYTPKLPLTAKALFPAGGIGLW 203 KGFD++E++LAV+K P +P +A A F G G W Sbjct: 153 GTKGFDKIEDILAVVKTAAIIMFIILAAAAMFGWLHSDADRPGIPDSAGAFFEDGFKGFW 212 Query: 204 SAFLFAFYAYGGIEVLGIMSYRLNKPEEAPKAGKVMLLTLTAVYVISIGLAVTMVPLSAF 263 S+ ++AFYAYGGIEV+G+M+ +L K E+APKAG VML+ L ++YVIS+GLAV MVP+ AF Sbjct: 213 SSLIYAFYAYGGIEVIGLMAMQLKKKEDAPKAGSVMLILLASIYVISLGLAVAMVPIDAF 272 Query: 264 NPKESPFVLALSSDHLAFVPHLFNGVLIIAGFSTMTASLYAVTSMMLTLAQEGDAPRLFS 323 + KESPF+ ALS +HL F PH+FNG +IIAGFSTMTASL+ VT++++TLA++GDAP F Sbjct: 273 HEKESPFITALSKNHLPFFPHVFNGAIIIAGFSTMTASLFGVTNLLVTLAKDGDAPAFFG 332 Query: 324 KKLK--GKYPLYALCLISGGLIAAIVMSLLLPGKVYEYITTAAGLMLLYNWSFILLSSGK 381 KK+K PL +L L S GL+ +I+ +LLLPGK+YEYITTAAG++LLYNW FI++SS K Sbjct: 333 KKIKKMKDLPLPSLGLASVGLLLSIITALLLPGKIYEYITTAAGILLLYNWFFIVISSFK 392 Query: 382 LLEPVKWSGLKRWTGLVLIVLAVAGTLFHKLSRPGLYISL 421 LL+ WS L L+LI+ AV+GTL K RPG ++SL Sbjct: 393 LLKAKIWSKLMGSAALLLILAAVSGTLLEKNIRPGFFVSL 432 >B7HWM0.1 B7HWM0_BACC7 Amino acid permease family protein Length = 447 Score = 399 bits (1024), Expect = e-109, Method: Compositional matrix adjust. Identities = 205/420 (48%), Positives = 282/420 (67%) Query: 24 VAGTIGTGYFLGSSLAISIGGPAVLLVYILAAAGTYVVFDALSRMTADHPEQGSFRSYAK 83 + TIGTG+FLGSS+ I IGG +++L+ IL TY+VF+AL++M + P++GSFR Y+K Sbjct: 26 IGCTIGTGFFLGSSIGIKIGGSSIILILILTGFTTYIVFEALAKMAVEDPQKGSFRIYSK 85 Query: 84 KAFGSWAGFASGWVYWFSELLIMGSQLTALAIFSRFWFPAVPMWXXXXXXXXXXXXXXXX 143 KAFG+WAGF+ GW YW SELLIMGSQL+AL IFSR+WFP +P+W Sbjct: 86 KAFGNWAGFSHGWTYWCSELLIMGSQLSALGIFSRYWFPKIPLWIFAAIYGVAAILIIFI 145 Query: 144 XNKGFDRVENVLAVIKXXXXXXXXXXXXXXXXXXXXXXKYTPKLPLTAKALFPAGGIGLW 203 K F+R+E +A+IK Y ++P K FP G G W Sbjct: 146 GVKIFERLEKWMAIIKIAAIMGFIVIAILVILGFIKGGLYKAQIPRNFKDWFPNGLKGTW 205 Query: 204 SAFLFAFYAYGGIEVLGIMSYRLNKPEEAPKAGKVMLLTLTAVYVISIGLAVTMVPLSAF 263 S+ ++AFYA+GGIE++G+M+ RL P++A K+G+VML+ LT +YV+S+GLA+ MVPL+ F Sbjct: 206 SSLIYAFYAFGGIEIMGLMTIRLKDPKDAQKSGRVMLMILTIIYVVSLGLAIMMVPLNQF 265 Query: 264 NPKESPFVLALSSDHLAFVPHLFNGVLIIAGFSTMTASLYAVTSMMLTLAQEGDAPRLFS 323 N KESPFV+AL +L +VP + N VLI+AGFST+ SL+AVTS+++ LA+EGDAP +F+ Sbjct: 266 NTKESPFVIALQDYNLPYVPFILNVVLILAGFSTLVGSLFAVTSVLMNLAEEGDAPAMFA 325 Query: 324 KKLKGKYPLYALCLISGGLIAAIVMSLLLPGKVYEYITTAAGLMLLYNWSFILLSSGKLL 383 KK K AL LI GL A+I+ +LLLP +YEY+TTAAGLMLLY WS ILLS +L+ Sbjct: 326 KKNKKNISFPALILIIMGLGASILSALLLPKNIYEYVTTAAGLMLLYTWSLILLSFWRLM 385 Query: 384 EPVKWSGLKRWTGLVLIVLAVAGTLFHKLSRPGLYISLLLVALIVGTDLIVHKVRSKAGS 443 P W +K GL++I LAV+GTL K +R G +SLL + +++ +IVH V K + Sbjct: 386 NPKGWMQIKSIIGLIMIALAVSGTLLQKTTRFGFLVSLLFLGIVIIVTIIVHFVSRKRNT 445 >B5V262.1 B5V262_BACCE Amino acid permease family protein Length = 447 Score = 399 bits (1024), Expect = e-109, Method: Compositional matrix adjust. Identities = 205/420 (48%), Positives = 282/420 (67%) Query: 24 VAGTIGTGYFLGSSLAISIGGPAVLLVYILAAAGTYVVFDALSRMTADHPEQGSFRSYAK 83 + TIGTG+FLGSS+ I IGG +++L+ IL TY+VF+AL++M + P++GSFR Y+K Sbjct: 26 IGCTIGTGFFLGSSIGIKIGGSSIILILILTGFTTYIVFEALAKMAVEDPQKGSFRIYSK 85 Query: 84 KAFGSWAGFASGWVYWFSELLIMGSQLTALAIFSRFWFPAVPMWXXXXXXXXXXXXXXXX 143 KAFG+WAGF+ GW YW SELLIMGSQL+AL IFSR+WFP +P+W Sbjct: 86 KAFGNWAGFSHGWTYWCSELLIMGSQLSALGIFSRYWFPKIPLWIFAAIYGVAAILIIFI 145 Query: 144 XNKGFDRVENVLAVIKXXXXXXXXXXXXXXXXXXXXXXKYTPKLPLTAKALFPAGGIGLW 203 K F+R+E +A+IK Y ++P K FP G G W Sbjct: 146 GVKIFERLEKWMAIIKIAAIMGFIVIAILVILGFIKGGLYKAQIPRNFKDWFPNGLKGTW 205 Query: 204 SAFLFAFYAYGGIEVLGIMSYRLNKPEEAPKAGKVMLLTLTAVYVISIGLAVTMVPLSAF 263 S+ ++AFYA+GGIE++G+M+ RL P++A K+G+VML+ LT +YV+S+GLA+ MVPL+ F Sbjct: 206 SSLIYAFYAFGGIEIMGLMTIRLKDPKDAQKSGRVMLMILTIIYVVSLGLAIMMVPLNQF 265 Query: 264 NPKESPFVLALSSDHLAFVPHLFNGVLIIAGFSTMTASLYAVTSMMLTLAQEGDAPRLFS 323 N KESPFV+AL +L +VP + N VLI+AGFST+ SL+AVTS+++ LA+EGDAP +F+ Sbjct: 266 NTKESPFVIALQDYNLPYVPFILNVVLILAGFSTLVGSLFAVTSVLMNLAEEGDAPAMFA 325 Query: 324 KKLKGKYPLYALCLISGGLIAAIVMSLLLPGKVYEYITTAAGLMLLYNWSFILLSSGKLL 383 KK K AL LI GL A+I+ +LLLP +YEY+TTAAGLMLLY WS ILLS +L+ Sbjct: 326 KKNKKNISFPALILIIMGLGASILSALLLPKNIYEYVTTAAGLMLLYTWSLILLSFWRLM 385 Query: 384 EPVKWSGLKRWTGLVLIVLAVAGTLFHKLSRPGLYISLLLVALIVGTDLIVHKVRSKAGS 443 P W +K GL++I LAV+GTL K +R G +SLL + +++ +IVH V K + Sbjct: 386 NPKGWMQIKSIIGLIMIALAVSGTLLQKTTRFGFLVSLLFLGIVIIVTIIVHFVSRKRNT 445 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.324 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,330,360,364 Number of extensions: 88863601 Number of successful extensions: 265569 Number of sequences better than 1.0e-05: 4263 Number of HSP's gapped: 260440 Number of HSP's successfully gapped: 6876 Length of query: 472 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 333 Effective length of database: 1,751,175,118 Effective search space: 583141314294 Effective search space used: 583141314294 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)