BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0065.p (424 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KGP0.1 B3KGP0_9BACI Major facilitator superfamily MFS_1 514 e-144 Q2B9G6.1 Q2B9G6_9BACI Multidrug resistance protein 399 e-109 Q9K7Q2.1 Q9K7Q2_BACHD Multidrug resistance protein 393 e-107 A6CHN6.1 A6CHN6_9BACI Multidrug resistance protein 390 e-106 Q5WDZ1.1 Q5WDZ1_BACSK Efflux transporter multidrug resistance pr... 378 e-103 >B3KGP0.1 B3KGP0_9BACI Major facilitator superfamily MFS_1 Length = 426 Score = 514 bits (1325), Expect = e-144, Method: Compositional matrix adjust. Identities = 255/394 (64%), Positives = 307/394 (77%), Gaps = 4/394 (1%) Query: 13 SIPLIMTLGNSMLLPILPQISNELGISAFQVSMIITVYGLIAILMIPIAGYLSDRFGRKK 72 SIPLIMTLGNSML+PILPQIS LGIS+F+VSM+ITVY ++AIL+IPIAGYLSDR+GRKK Sbjct: 13 SIPLIMTLGNSMLIPILPQISKVLGISSFKVSMLITVYAVVAILLIPIAGYLSDRYGRKK 72 Query: 73 VXXXXXXXXXXXXXXXXXXXWFMNGVSAYWVILAGRFIQGIGAAGAFPIVIPFIGDLFKD 132 + W ++G AY+VILAGRF+QGIGAAGAFPIVIP +GD+FK+ Sbjct: 73 IIIPSLIIAALGGAVSAVAAWLLSGTGAYYVILAGRFLQGIGAAGAFPIVIPLVGDMFKE 132 Query: 133 EKEVSKGLGVVETSNTFGKVLSPILGAYLGTVVWYAPFMAIPILCLISFGLVVFLVKKPE 192 E+EVSK LG++ETSNTFGKV+SPILGA LG+ +W+ PF++IPILCLIS LV+FLVK P+ Sbjct: 133 EEEVSKSLGIIETSNTFGKVVSPILGALLGSFIWFLPFISIPILCLISLVLVIFLVKTPK 192 Query: 193 AGEQPEKKALRRFLGEIKDILHERGRWLYAIFAIGGICMFVTFGVLFYLSETLEAKYNLH 252 + +K++LR FL K +LHE+GRWLYAIFAIGGI MF FGVLFYLSETLE+ Y LH Sbjct: 193 SNNN-DKQSLREFLKTTKGVLHEKGRWLYAIFAIGGIAMFGVFGVLFYLSETLESTYKLH 251 Query: 253 GSSKGFXXXXXXXXXXXXSYGSGKIIGQNKALMKWLGFGGMVLLTAAMIITGFNKNIYFM 312 G KG SY +GK G+NKA KW GF GMV+LTA++I+TGF++ IYF+ Sbjct: 252 GVVKGLVLAIPLALLCLSSYLAGKFTGKNKAAKKWTGFAGMVILTASLIVTGFSEQIYFV 311 Query: 313 VGFLSLSGIGIGVALPCMDALITESIEKENRGTITSLYSSMRFIGVALGPPVVSLLMTRG 372 VG ++L GIGIG+ LPCMD L+TE +EKE RGTITSLYSSMRFIGV+LGPPVVSLL+ Sbjct: 312 VGMMTLGGIGIGMVLPCMDTLLTEGVEKEQRGTITSLYSSMRFIGVSLGPPVVSLLLGAN 371 Query: 373 HWVLFGTMAATGAVGGLLTLFAVKPSEGGNKEDK 406 HWV+FGT+AA AVGGLLTLFAVKP KED Sbjct: 372 HWVMFGTLAAVAAVGGLLTLFAVKPEA---KEDD 402 >Q2B9G6.1 Q2B9G6_9BACI Multidrug resistance protein Length = 423 Score = 399 bits (1025), Expect = e-109, Method: Compositional matrix adjust. Identities = 204/389 (52%), Positives = 265/389 (68%), Gaps = 4/389 (1%) Query: 13 SIPLIMTLGNSMLLPILPQISNELGISAFQVSMIITVYGLIAILMIPIAGYLSDRFGRKK 72 SIPL+MTLGNSML+P+LP + ELGIS+FQ SMIITVY ++AI++IP+AGYLSD GRKK Sbjct: 39 SIPLVMTLGNSMLIPVLPVMERELGISSFQSSMIITVYSIVAIILIPLAGYLSDHIGRKK 98 Query: 73 VXXXXXXXXXXXXXXXXXXXWFMNGVSAYWVILAGRFIQGIGAAGAFPIVIPFIGDLFKD 132 V W M YW+IL GR +QGIGAAG PIV+P +GD+FK+ Sbjct: 99 VIIPSLLIAAVGGLISGWASWKM--ADPYWLILIGRSLQGIGAAGGAPIVMPLVGDMFKN 156 Query: 133 EKEVSKGLGVVETSNTFGKVLSPILGAYLGTVVWYAPFMAIPILCLISFGLVVFLVKKPE 192 E+EVS LGV+ETSNTFGKVLSPILGA+L +W+ PF A P+ C IS L+V LVK PE Sbjct: 157 EEEVSSSLGVIETSNTFGKVLSPILGAFLAGFLWFLPFFAFPVFCAISILLMVLLVKTPE 216 Query: 193 AGEQPEKKALRRFLGEIKDILHERGRWLYAIFAIGGICMFVTFGVLFYLSETLEAKYNLH 252 +P + F +IK+I G+WLYAIF G I MFV FGVLF+LS+ LE +Y++ Sbjct: 217 KRTEP--IPFKAFFKKIKEIFKNNGKWLYAIFVAGIILMFVLFGVLFHLSDILEKQYDIK 274 Query: 253 GSSKGFXXXXXXXXXXXXSYGSGKIIGQNKALMKWLGFGGMVLLTAAMIITGFNKNIYFM 312 KG SY +GK+I +NK+LMKW+ F G +LL A++ I GF+ N++F+ Sbjct: 275 DVKKGLFLAIPLGALCLSSYITGKLIKENKSLMKWITFSGSILLAASVGILGFSTNLWFL 334 Query: 313 VGFLSLSGIGIGVALPCMDALITESIEKENRGTITSLYSSMRFIGVALGPPVVSLLMTRG 372 + ++ GIGIGV+LPC+DALITE IEKE RGTI+SLYSSMRFIGVA GPP V++++ + Sbjct: 335 MAMFAVGGIGIGVSLPCLDALITEGIEKEERGTISSLYSSMRFIGVAAGPPAVAIIVKQS 394 Query: 373 HWVLFGTMAATGAVGGLLTLFAVKPSEGG 401 +LF + + L LFA+KP + G Sbjct: 395 DQLLFYILCGVSILAALAVLFAIKPDKEG 423 >Q9K7Q2.1 Q9K7Q2_BACHD Multidrug resistance protein Length = 403 Score = 393 bits (1010), Expect = e-107, Method: Compositional matrix adjust. Identities = 204/388 (52%), Positives = 261/388 (67%), Gaps = 4/388 (1%) Query: 13 SIPLIMTLGNSMLLPILPQISNELGISAFQVSMIITVYGLIAILMIPIAGYLSDRFGRKK 72 SIPL+MTLGNSML+P+LP I EL IS+ QVS++ITVY ++AI+ IPIAGYLSD GRKK Sbjct: 14 SIPLVMTLGNSMLIPVLPIIERELDISSLQVSLLITVYSVVAIIFIPIAGYLSDIIGRKK 73 Query: 73 VXXXXXXXXXXXXXXXXXXXWFMNGVSAYWVILAGRFIQGIGAAGAFPIVIPFIGDLFKD 132 V W S Y +IL GR +QGIGAAGA PIV+P +GDLF+ Sbjct: 74 VIIPSLILAAIGGVISGFAAW--KSPSPYTMILVGRLLQGIGAAGAAPIVMPLVGDLFQQ 131 Query: 133 EKEVSKGLGVVETSNTFGKVLSPILGAYLGTVVWYAPFMAIPILCLISFGLVVFLVKKPE 192 EKEVS+GLG++ETSNTFGKVLSPILGAYL T+VWY PF AIP+ CL S L++FLVK + Sbjct: 132 EKEVSRGLGIIETSNTFGKVLSPILGAYLATIVWYLPFWAIPLFCLFSILLMLFLVKTEK 191 Query: 193 AGEQPEKKALRRFLGEIKDILHERGRWLYAIFAIGGICMFVTFGVLFYLSETLEAKYNLH 252 ++P + F+ IK I GRWLYA+F +G ICMF+ FG+LFYLS LE +Y + Sbjct: 192 ETKEPVP--FQTFIQTIKTIFKREGRWLYAVFVVGCICMFLLFGILFYLSTMLEERYQIE 249 Query: 253 GSSKGFXXXXXXXXXXXXSYGSGKIIGQNKALMKWLGFGGMVLLTAAMIITGFNKNIYFM 312 G KG S +G+ I ++KA MKW+ G+ LLTA+ F+++IY + Sbjct: 250 GVKKGVVLAIPLAALCIASLATGRWIEEDKAKMKWMSVLGLALLTASTAWIIFSESIYVL 309 Query: 313 VGFLSLSGIGIGVALPCMDALITESIEKENRGTITSLYSSMRFIGVALGPPVVSLLMTRG 372 + L +SGIGIG+ LP +DAL+TE I KE RGTITSLY+SMRF+GVA GPP+ +LLM Sbjct: 310 LAGLFISGIGIGMTLPSLDALVTEGIHKEQRGTITSLYNSMRFVGVAFGPPLFALLMKTS 369 Query: 373 HWVLFGTMAATGAVGGLLTLFAVKPSEG 400 H + F A T + LL L+A++P EG Sbjct: 370 HSITFMVNAITAFLTMLLILWAIRPEEG 397 >A6CHN6.1 A6CHN6_9BACI Multidrug resistance protein Length = 428 Score = 390 bits (1001), Expect = e-106, Method: Compositional matrix adjust. Identities = 213/403 (52%), Positives = 279/403 (69%), Gaps = 14/403 (3%) Query: 13 SIPLIMTLGNSMLLPILPQISNELGISAFQVSMIITVYGLIAILMIPIAGYLSDRFGRKK 72 SIPL+MTLGNSML+P+LP + +LGIS FQ SMIITVY L+AIL+IPIAGYLSDRFGRKK Sbjct: 35 SIPLVMTLGNSMLIPVLPTMEKKLGISGFQSSMIITVYSLVAILLIPIAGYLSDRFGRKK 94 Query: 73 VXXXXXXXXXXXXXXXXXXXWFMNGVSAYWVILAGRFIQGIGAAGAFPIVIPFIGDLFKD 132 + W MN AYWV+L GRF+QGIGAAGAFP+VIP +GD+F+D Sbjct: 95 IIIPSLIIAGIGGIISGWASWMMN--DAYWVVLIGRFVQGIGAAGAFPVVIPTVGDMFED 152 Query: 133 EKEVSKGLGVVETSNTFGKVLSPILGAYLGTVVWYAPFMAIPILCLISFGLVVFLVKKPE 192 E EV+KGLG++ETSNTFGKVLSP+LG+ L +W+ PF +IP+ LI+ GLV FLVK P Sbjct: 153 ESEVTKGLGLIETSNTFGKVLSPVLGSLLAAWIWFIPFFSIPLFSLIAIGLVFFLVKVPR 212 Query: 193 AGEQPEK-KALRRFLGEIKDILHERGRWLYAIFAIGGICMFVTFGVLFYLSETLEAKYNL 251 + +K ++++ F+ EIK+IL + G+WL AIF IG + MF+ FG LF+LS LE+KY + Sbjct: 213 KEKSDDKGQSVKEFMKEIKEILAKEGKWLSAIFLIGIVSMFILFGFLFFLSNVLESKYKI 272 Query: 252 HGSSKGFXXXXXXXXXXXXSYGSGKIIGQNKALMKWLGFGGMVLLTAAMIITGFNKNIYF 311 +G KG SY +GK IG+N+++MKW GM+L A + F + Sbjct: 273 NGVLKGLYLAMPLLFLCATSYLTGKFIGKNRSMMKWAVVSGMLLGAGAQVPIFFTDGKWM 332 Query: 312 MVGFLSLSGIGIGVALPCMDALITESIEKENRGTITSLYSSMRFIGVALGPPVVSLLMTR 371 ++ FLSL+ IGIG ALPC+DALITE IEKE+RGTITS+YSSMRFIGVA GPP+V+LL ++ Sbjct: 333 LLIFLSLASIGIGAALPCLDALITEGIEKEHRGTITSIYSSMRFIGVAAGPPIVALL-SK 391 Query: 372 GHWVLFGTMAATGAVGGLLTLFAVKPSEGGNKEDKPLKSGRVR 414 G V F + AV + L+ +KP +P KSG+++ Sbjct: 392 G--VTFMILIGVSAVSAFVALWLIKP--------EPEKSGKMQ 424 >Q5WDZ1.1 Q5WDZ1_BACSK Efflux transporter multidrug resistance protein Length = 406 Score = 378 bits (970), Expect = e-103, Method: Compositional matrix adjust. Identities = 188/387 (48%), Positives = 256/387 (66%), Gaps = 4/387 (1%) Query: 13 SIPLIMTLGNSMLLPILPQISNELGISAFQVSMIITVYGLIAILMIPIAGYLSDRFGRKK 72 S+PL+MTLGNSML+P+LP I +L IS+F VS++ITVY ++AI+ IPIAGY+SD GRK Sbjct: 17 SVPLVMTLGNSMLIPVLPTIGKQLQISSFMVSLLITVYSVVAIVFIPIAGYISDHVGRKP 76 Query: 73 VXXXXXXXXXXXXXXXXXXXWFMNGVSAYWVILAGRFIQGIGAAGAFPIVIPFIGDLFKD 132 V W M+ +AYWVIL GR +QGIGAAGA PIV+PF+GDLFK+ Sbjct: 77 VIVPSLIIAGIGGLMAGLAAWKMD--NAYWVILIGRLLQGIGAAGASPIVMPFVGDLFKN 134 Query: 133 EKEVSKGLGVVETSNTFGKVLSPILGAYLGTVVWYAPFMAIPILCLISFGLVVFLVKKPE 192 ++VS+GLG++ET+NTFGKVLSPI GA L +++WY PF + PI C +S LV+FLV+ P Sbjct: 135 NQDVSEGLGIIETANTFGKVLSPIFGAVLASIIWYLPFFSFPIFCFVSIFLVLFLVESPP 194 Query: 193 AGEQPEKKALRRFLGEIKDILHERGRWLYAIFAIGGICMFVTFGVLFYLSETLEAKYNLH 252 ++P L RFL +K I GRWLY +F +GG+CM++ FGVLFYLS LE +++ Sbjct: 195 LAKKPTN--LTRFLKSMKKIFKREGRWLYVVFFVGGMCMYILFGVLFYLSSFLEEAHHID 252 Query: 253 GSSKGFXXXXXXXXXXXXSYGSGKIIGQNKALMKWLGFGGMVLLTAAMIITGFNKNIYFM 312 G KG SYG+G+IIGQ++ MKW+ G+ LL + + FNK +Y M Sbjct: 253 GIRKGVILAFPLMALCLTSYGTGRIIGQDQIKMKWMTVAGLCLLAVSTFVVSFNKELYVM 312 Query: 313 VGFLSLSGIGIGVALPCMDALITESIEKENRGTITSLYSSMRFIGVALGPPVVSLLMTRG 372 + L +G+GIG ALP +DA +TE I E RG++TS Y+SMRF+GVA+GPP+ +L M Sbjct: 313 LTALFAAGVGIGAALPSLDAFVTEGIPMEQRGSVTSFYNSMRFVGVAIGPPLFALFMKGN 372 Query: 373 HWVLFGTMAATGAVGGLLTLFAVKPSE 399 H+ F A + LL + V+P + Sbjct: 373 HFWTFAGNALLVVLSVLLVFWGVRPHK 399 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.325 0.144 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,288,192,055 Number of extensions: 92203478 Number of successful extensions: 410909 Number of sequences better than 1.0e-05: 1625 Number of HSP's gapped: 411858 Number of HSP's successfully gapped: 1626 Length of query: 424 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 286 Effective length of database: 1,760,596,133 Effective search space: 503530494038 Effective search space used: 503530494038 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 133 (55.8 bits)