BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0064.p (621 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K6L2.1 B3K6L2_9BACI Alpha amylase catalytic region 723 0.0 B1DH60.1 B1DH60_9BACL Alpha amylase catalytic region 719 0.0 Q84AP9.1 Q84AP9_9BACL Cyclodextrinase enzyme 623 e-176 B3K4I1.1 B3K4I1_9BACI Alpha amylase catalytic region 623 e-176 O69007.1 O69007_9DEIN Maltogenic amylase 608 e-172 >B3K6L2.1 B3K6L2_9BACI Alpha amylase catalytic region Length = 582 Score = 723 bits (1865), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/577 (58%), Positives = 431/577 (74%), Gaps = 2/577 (0%) Query: 26 EALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEHYHEEHRMQKIASDQ 85 EAL+HVP WAY YD T HLR+RT K ++D V A+TGDKYDW+ + E M+K ASD Sbjct: 4 EALYHVPRDKWAYAYDTHTIHLRIRTKKNDIDSVVAMTGDKYDWDRTYFEITMEKAASDD 63 Query: 86 FFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYYERPYIHEADLFK 145 FDYWE V+P+ KR SY FR+ AGDET++M ++G + P PP GY+E PYIHE D+FK Sbjct: 64 MFDYWECGVRPKYKRLSYGFRIHAGDETVYMVDSGIGWDYPYPPAGYFEFPYIHEVDVFK 123 Query: 146 APEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDTPTAESFFGGDLQGIMDHLDYLARL 205 PEWAK AVFYQIM +RFANG+P P AWG P ++FFGGDLQG++DHLD L L Sbjct: 124 VPEWAKEAVFYQIMTERFANGNPAISPDGTEAWGGKPEVDNFFGGDLQGVLDHLDDLVDL 183 Query: 206 GITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVVLDAVFNHI 265 GI AIY TP+F +PSNHKYDT DY++VDP+FGD +LLKK+V T HAKGIRV+LDAVFNH Sbjct: 184 GINAIYFTPLFLSPSNHKYDTVDYKQVDPHFGDNDLLKKVVDTCHAKGIRVILDAVFNHC 243 Query: 266 SAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLNTTNPEARK 325 S + P FQ V++HGE+S+YAGWFH++ FP ++ DG P YD FGFF +MPK NT NPE + Sbjct: 244 SEEFPPFQDVLKHGEKSKYAGWFHVNRFPCEIKDGIPTYDTFGFFGNMPKFNTANPEVKA 303 Query: 326 YLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVWSDSMRWLN 385 YLL A YW++E+ +DGWRLDVANEIDH FWRDFR++VK NPEA+I+GEVWSDS+ WL Sbjct: 304 YLLGVAEYWIKEIKLDGWRLDVANEIDHHFWRDFRQVVKKANPEAYIVGEVWSDSLIWLQ 363 Query: 386 GDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNLLASHDTPR 445 GDQFDSVMNYP +++E+ NG+++ F ++G++LMRYPQQ NEV+FNLL SHDTPR Sbjct: 364 GDQFDSVMNYPFADKVLEFF-NGNMDGLTFANRIGSILMRYPQQTNEVVFNLLCSHDTPR 422 Query: 446 ILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEGERQNRDLF 505 +LT++G D ++KL FLFTF GTPCIFYGDEIGL G+GDPDCRKCM W+ +++N++L+ Sbjct: 423 LLTRVGEDIRRMKLTVVFLFTFMGTPCIFYGDEIGLTGDGDPDCRKCMEWDPKKRNQELY 482 Query: 506 ESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSEQPATLRLP 565 YQ +I LRK AL+ GRFR L A+ VYER DE HF++ +N S+ TL P Sbjct: 483 AFYQGMIALRKEHKALRQGRFRFLQANLHDPCIVYERADETMHFIVWINNSDWKRTLSHP 542 Query: 566 L-EDRFQDALNGEKLIPSDGQMVLDLAPYSYKILIKE 601 + + DA E + P +G M +DL PY+Y+IL +E Sbjct: 543 IVTTDWIDAETQEPVKPVNGTMHIDLEPYAYRILCRE 579 >B1DH60.1 B1DH60_9BACL Alpha amylase catalytic region Length = 582 Score = 719 bits (1857), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/582 (56%), Positives = 428/582 (73%), Gaps = 1/582 (0%) Query: 23 IQREALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEHYHEEHRMQKIA 82 +QRE+++H P W+Y YD TFHLR++T +++V++V A+ GDKYDW+H+++E M+ IA Sbjct: 1 MQRESIYHSPSHRWSYAYDPGTFHLRLKTKRSDVERVVAIAGDKYDWDHHYQEVEMELIA 60 Query: 83 SDQFFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYYERPYIHEAD 142 +D DYWE ++PE KRFSY FR+ +G+ET W+TE GF E P P GY+E YIH D Sbjct: 61 ADGMHDYWEAVIRPEFKRFSYGFRLHSGEETAWLTEDGFSEEQPTPAGGYFEAHYIHPID 120 Query: 143 LFKAPEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDTPTAESFFGGDLQGIMDHLDYL 202 LF+APEWAK A+FYQI P+RF NGD DP + WG+ P ESFFGGDLQGI++ + +L Sbjct: 121 LFEAPEWAKEAIFYQIYPERFENGDTTNDPEGTSPWGEQPEGESFFGGDLQGIINRIGHL 180 Query: 203 ARLGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVVLDAVF 262 LG+ A+YLTP+F +PSNHKYDTTDYR+VDP+FGD +LLK LV H GIRVVLDAVF Sbjct: 181 NELGVNAVYLTPVFRSPSNHKYDTTDYREVDPHFGDKDLLKMLVEVCHKHGIRVVLDAVF 240 Query: 263 NHISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLNTTNPE 322 NH S Q P FQ V+E G+QSE+ WFH++ FPV+V DG NYD FGF+ +MPKLNT NP+ Sbjct: 241 NHASEQFPPFQDVLEKGDQSEFKDWFHLNGFPVEVQDGIANYDTFGFYGNMPKLNTANPD 300 Query: 323 ARKYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVWSDSMR 382 + YL++TA W++E GIDGWRLDVANEIDH FWRDFR +KD N EAFIIGEVWSDS+R Sbjct: 301 VKNYLIETAVNWMKETGIDGWRLDVANEIDHHFWRDFRAAIKDANKEAFIIGEVWSDSLR 360 Query: 383 WLNGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNLLASHD 442 WL GDQFDSVMNYP T ++ + + I RF ++ LLMRYPQQ NE LFNLL+SHD Sbjct: 361 WLLGDQFDSVMNYPFTELVLSFFADRAIPPARFAEKMNRLLMRYPQQTNETLFNLLSSHD 420 Query: 443 TPRILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEGERQNR 502 PR++T+ GGD +LK A ++ T G PC++YGDE G+EG DPDCR+CM+W E QNR Sbjct: 421 IPRVMTRCGGDTKRLKQAVVYMMTSMGLPCVYYGDEFGMEGGEDPDCRRCMVWNEEDQNR 480 Query: 503 DLFESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSEQPATL 562 +LF+ Y+ LI LRK AL++GRFR+L A+ + +YER+D HF I +N S++PA L Sbjct: 481 ELFDFYKLLISLRKERAALRSGRFRVLLAEEDNGSLIYERLDGKEHFTIWMNRSDEPAEL 540 Query: 563 RLPL-EDRFQDALNGEKLIPSDGQMVLDLAPYSYKILIKETD 603 P+ E + DAL GE++ DG++V+ L P Y+I+ ++ + Sbjct: 541 SHPMNEGGWSDALTGERIDNPDGKLVIGLEPNGYRIVWRKIE 582 >Q84AP9.1 Q84AP9_9BACL Cyclodextrinase enzyme Length = 653 Score = 623 bits (1607), Expect = e-176, Method: Compositional matrix adjust. Identities = 294/588 (50%), Positives = 396/588 (67%), Gaps = 7/588 (1%) Query: 26 EALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEHYHEEHRMQKIASDQ 85 EA++H P NW+Y Y+ T HLR+RT K ++ V+AL GDKY W+H E M K+A+D+ Sbjct: 4 EAVYHRPRKNWSYAYNGTTVHLRIRTKKDDMTAVYALAGDKYMWDHTMEYVPMTKLATDE 63 Query: 86 FFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYYERPYIHEADLFK 145 FDYWE V P +R Y F +Q G E WMTE F TE PA P+ +E P+I+ D+F+ Sbjct: 64 LFDYWECEVTPPYRRVKYGFLLQQGHEKRWMTEYDFLTEPPANPDRLFEYPFINPVDVFQ 123 Query: 146 APEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDT-PTAESFFGGDLQGIMDHLDYLAR 204 P W K A+FYQI P+RFANGD DP WG PT FFGGDLQG++DHLD+L++ Sbjct: 124 PPAWVKDAIFYQIFPERFANGDTRNDPEGTLPWGSADPTPSCFFGGDLQGVIDHLDHLSK 183 Query: 205 LGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVVLDAVFNH 264 LG+ A+Y TP+F+A +NHKYDT DY ++DP FGD + LKKLV H +GIRV+LDAVFNH Sbjct: 184 LGVNAVYFTPLFKATTNHKYDTEDYFQIDPQFGDKDTLKKLVDLCHERGIRVLLDAVFNH 243 Query: 265 ISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLNTTNPEAR 324 P F V+++GE+S+Y WFHI + P++V DG P YD F F MPKLNT +PE + Sbjct: 244 SGRTFPPFVDVLKNGEKSKYKDWFHIRSLPLEVVDGIPTYDTFAFEPLMPKLNTEHPEVK 303 Query: 325 KYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVWSDSMRWL 384 +YLL A YW+ E GIDGWRLDVANE+ H FWR+FR++VK NP+A+I+G+VW +S WL Sbjct: 304 EYLLKAAEYWIRETGIDGWRLDVANEVSHQFWREFRRVVKQANPDAYILGDVWHESSIWL 363 Query: 385 NGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNLLASHDTP 444 GDQFD+VMNYP T+ ++++ + +A +F LG L YP+QA+EV+FNLL SHDT Sbjct: 364 EGDQFDAVMNYPFTNAVLDFFIHQIADAEKFSFMLGKQLAGYPRQASEVMFNLLDSHDTA 423 Query: 445 RILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEGERQNRDL 504 R+LTQ GD K+KLA F FT+ GTPCI+YGDE+GL+G DP CRKCM W+ + ++DL Sbjct: 424 RLLTQADGDKRKMKLAVLFQFTYFGTPCIYYGDEVGLDGGHDPGCRKCMEWDETKHDKDL 483 Query: 505 FESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSEQPATLRL 564 F YQ++IRLR+A AL+TG F+ L+A+ R Y R D+ ++ +N + TL L Sbjct: 484 FAFYQTVIRLRQAHAALRTGTFKFLTAEKNSRQIAYLREDDQDTILVVMNNDKAGHTLTL 543 Query: 565 PLED-RFQDALNGEKLIPSDGQMVLDLAPYSYKILIKETD-----RPP 606 P+ ++ N + L + GQ+ + L Y + +L +D RPP Sbjct: 544 PVRHAQWTHLWNDDVLTAAHGQLTVKLPAYGFAVLKASSDWSSWGRPP 591 >B3K4I1.1 B3K4I1_9BACI Alpha amylase catalytic region Length = 582 Score = 623 bits (1606), Expect = e-176, Method: Compositional matrix adjust. Identities = 296/575 (51%), Positives = 389/575 (67%), Gaps = 2/575 (0%) Query: 26 EALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEHYHEEHRMQKIASDQ 85 EA++H P NW+Y Y+ T HLR+RT K ++ +V+AL GDKY W+ E M K++SD+ Sbjct: 4 EAIYHRPRKNWSYAYNGTTIHLRIRTKKDDLTEVYALAGDKYLWDQTMEYIPMTKLSSDE 63 Query: 86 FFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYYERPYIHEADLFK 145 FDYWE P +R Y F +Q GDE WMTE F TE PA P+ +E P+I+ D+F Sbjct: 64 MFDYWECEATPPYRRLKYGFLLQQGDEKRWMTEYDFLTEPPANPDRLFEYPFINPDDVFH 123 Query: 146 APEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWGDT-PTAESFFGGDLQGIMDHLDYLAR 204 P W K A+FYQI P+RFANGD DP WG PT ++FFGGDLQG++DHLD+L+ Sbjct: 124 PPAWVKDAIFYQIFPERFANGDTSNDPEGALPWGSAEPTPKNFFGGDLQGVIDHLDHLSE 183 Query: 205 LGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVVLDAVFNH 264 LG+ AIY TP+F A +NHKYDT DY ++DP FGD + LKKLV HA+GIRV+LDAVFNH Sbjct: 184 LGVNAIYFTPLFTATTNHKYDTEDYMEIDPQFGDKDTLKKLVDLCHARGIRVLLDAVFNH 243 Query: 265 ISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLNTTNPEAR 324 F V ++G S+Y WF++ + P++V DG P YD F F MPKLNT NPE R Sbjct: 244 SGRTFAPFVDVQKNGLNSKYKDWFYVRSLPLEVVDGIPTYDTFAFEPLMPKLNTENPEVR 303 Query: 325 KYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVWSDSMRWL 384 YLL A YW++E GIDGWRLDVANE+ H+FWR+FR++VK NP+A+I+GEVW +S WL Sbjct: 304 AYLLKAAEYWIKETGIDGWRLDVANEVGHSFWREFRQVVKKANPDAYILGEVWHESSIWL 363 Query: 385 NGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNLLASHDTP 444 GDQFD+VMNYP T+ ++++ N +A +F LG L YP+QA+EV+FNLL SHDT Sbjct: 364 EGDQFDAVMNYPFTNAVLDFFINRTADAEKFSFMLGKQLAGYPRQASEVMFNLLDSHDTA 423 Query: 445 RILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEGERQNRDL 504 R+LTQ GD +KLAA F FT+ GTPCI+YGDEIG++G DP CRKCM W+ + ++DL Sbjct: 424 RLLTQANGDKRLMKLAAMFQFTYFGTPCIYYGDEIGMDGGHDPGCRKCMEWDESKHDKDL 483 Query: 505 FESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSEQPATLRL 564 F YQ+LI+LRK AL+TG F+ L A+ Y R D+N +I++N + T+ L Sbjct: 484 FHFYQTLIKLRKEHSALRTGTFKFLKAEKNGGKIAYLREDDNETILIAVNNDKAGNTMTL 543 Query: 565 PLEDR-FQDALNGEKLIPSDGQMVLDLAPYSYKIL 598 P+ DR + + NGE L GQ+ + L Y + IL Sbjct: 544 PVLDRQWTNLWNGEGLSAKQGQLTVKLPAYGFAIL 578 >O69007.1 O69007_9DEIN Maltogenic amylase Length = 588 Score = 608 bits (1569), Expect = e-172, Method: Compositional matrix adjust. Identities = 296/588 (50%), Positives = 387/588 (65%), Gaps = 9/588 (1%) Query: 23 IQREALHHVPHSNWAYLYDKDTFHLRVRTGKANVDQVFALTGDKYDWEH---YHEEHRMQ 79 +++EA+HH N+AY YD +T HLR++T K +VD V L GD Y+W + M+ Sbjct: 1 MRKEAIHHRSTDNFAYAYDSETLHLRLQTKKNDVDHVELLFGDPYEWHDGAWQFQTMPMR 60 Query: 80 KIASDQFFDYWETAVKPENKRFSYAFRMQAGDETLWMTETGFFTELPAPPEGYY-ERPYI 138 K SD FDYW VKP +R Y F ++AG E L TE GF+ E P+ YY P++ Sbjct: 61 KTGSDGLFDYWLAEVKPPYRRLRYGFVLRAGGEKLVYTEKGFYHEAPSDDTAYYFCFPFL 120 Query: 139 HEADLFKAPEWAKSAVFYQIMPDRFANGDPGKDPVNVAAWG-DTPTAESFFGGDLQGIMD 197 H DLF+AP+W K V+YQI P+RFANG+P P WG + PT SFFGGDLQGI+D Sbjct: 121 HRVDLFQAPDWVKDTVWYQIFPERFANGNPAISPKGARPWGSEDPTPTSFFGGDLQGIID 180 Query: 198 HLDYLARLGITAIYLTPIFEAPSNHKYDTTDYRKVDPNFGDVELLKKLVTTAHAKGIRVV 257 HLDYLA LGIT IYLTPIF APSNHKYDT DY ++DP+FGD E LK LV H KGIRV+ Sbjct: 181 HLDYLADLGITGIYLTPIFRAPSNHKYDTADYFEIDPHFGDKETLKTLVKRCHEKGIRVM 240 Query: 258 LDAVFNHISAQSPQFQSVIEHGEQSEYAGWFHIHNFPVKVTDGKPNYDAFGFFADMPKLN 317 LDAVFNH + FQ V+++G S Y WFHI FP++ T+ +PNYD F F MPKLN Sbjct: 241 LDAVFNHCGYEFAPFQDVLKNGAASRYKDWFHIREFPLQ-TEPRPNYDTFAFVPHMPKLN 299 Query: 318 TTNPEARKYLLDTAGYWLEEVGIDGWRLDVANEIDHTFWRDFRKLVKDINPEAFIIGEVW 377 T +PE ++YLLD A YW+ E IDGWRLDVANEIDH FWR+FR+ VK + P+ +I+GE+W Sbjct: 300 TAHPEVKRYLLDVATYWIREFDIDGWRLDVANEIDHQFWREFRQAVKALKPDVYILGEIW 359 Query: 378 SDSMRWLNGDQFDSVMNYPLTSRLIEYLNNGDINAGRFGGQLGNLLMRYPQQANEVLFNL 437 D+M WL GDQFD+VMNYPL + + D++A F +L ++L YP+Q NE FNL Sbjct: 360 HDAMPWLRGDQFDAVMNYPLADAALRFFAKEDMSASEFADRLMHVLHSYPKQVNEAAFNL 419 Query: 438 LASHDTPRILTQLGGDPGKLKLAAAFLFTFTGTPCIFYGDEIGLEGEGDPDCRKCMIWEG 497 L SHDTPR+LT GGD K+KL F TFTG+PCI+YGDEIG+ G DP+CRKCM+W+ Sbjct: 420 LGSHDTPRLLTVCGGDVRKVKLLFLFQLTFTGSPCIYYGDEIGMTGGNDPECRKCMVWDP 479 Query: 498 ERQNRDLFESYQSLIRLRKAFPALQTGRFRILSADPQQRAFVYERVDENAHFVISLNPSE 557 E+QN++L+E + LI LRK + AL+ G L+AD + VY + D N +I +N S Sbjct: 480 EKQNKELYEHVKQLIALRKQYRALRRGDVAFLTADDEVNHLVYAKTDGNETVMIIINRSN 539 Query: 558 QPATLRLPLEDR---FQDALNGEKLIPSDGQMVLDLAPYSYKILIKET 602 + A + +P++ R + L GE+ + + L PY + + E+ Sbjct: 540 EAAEIPMPIDARGKWLVNLLTGERFAAEAETLCVSLPPYGFVLYAVES 587 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.138 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 4,232,142,403 Number of extensions: 194949730 Number of successful extensions: 407172 Number of sequences better than 1.0e-05: 5259 Number of HSP's gapped: 395158 Number of HSP's successfully gapped: 5526 Length of query: 621 Length of database: 3,060,696,203 Length adjustment: 142 Effective length of query: 479 Effective length of database: 1,722,912,073 Effective search space: 825274882967 Effective search space used: 825274882967 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)