BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0054.p (188 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C0BCG0.1 C0BCG0_9FIRM Putative uncharacterized protein 105 2e-21 C3EMK6.1 C3EMK6_BACTK Transcriptional regulator, TetR 102 1e-20 C2XDL4.1 C2XDL4_BACCE Transcriptional regulator, TetR 102 1e-20 C2P0K2.1 C2P0K2_BACCE Transcriptional regulator, TetR 102 1e-20 C0FR56.1 C0FR56_9FIRM Putative uncharacterized protein 102 2e-20 >C0BCG0.1 C0BCG0_9FIRM Putative uncharacterized protein Length = 188 Score = 105 bits (262), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 53/173 (30%), Positives = 95/173 (54%), Gaps = 1/173 (0%) Query: 10 LKCFGAKGTRFTTEDLARELGTSKRTVYTYFSSKDEMIEKTIDFVFSQIIQSDTDILEDE 69 +K F KG +FT +DLA LG SK+T+YT F K+ ++ + +D+ FS I +S+ +L+D Sbjct: 15 IKAFQKKGLKFTMDDLASLLGISKKTIYTVFPDKNSLVLEMVDYCFSSIKESEQKVLQDL 74 Query: 70 GLPIQEKIRLYFQNIPDAYSIGTIIRHMDDFQRYYPRLYEKANHHLDRIWDGVIELVEEG 129 L KIR +P+ Y +R + + YP +YEK L+ W+ I L+E+G Sbjct: 75 SLDTVGKIRAILGVLPEGYR-ELDLRQLYQLKERYPEVYEKVKSRLETGWESTISLLEQG 133 Query: 130 MNRGELHKVDTVILKLMLNETLKKLLDYEFIAGHQVSFESGIKAMSDIVLYGL 182 + G++ + ILK M+ TL++ + + + +S+ + + I++ G+ Sbjct: 134 ITEGKIRNIQIPILKTMMEATLEQFFQRDVLVQNGISYHEALDEVVSILIDGI 186 >C3EMK6.1 C3EMK6_BACTK Transcriptional regulator, TetR Length = 186 Score = 102 bits (254), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 53/182 (29%), Positives = 101/182 (55%), Gaps = 1/182 (0%) Query: 1 MDNQVYLKILKCFGAKGTRFTTEDLARELGTSKRTVYTYFSSKDEMIEKTIDFVFSQIIQ 60 M +++ + F +G +FT DLAR+LG SK T+Y +F SKDE+I ++ + + Sbjct: 2 MKDRIAKTAIALFHVRGIKFTMSDLARDLGVSKSTLYEHFPSKDELIGYVVEQINEEAQI 61 Query: 61 SDTDILEDEGLPIQEKIRLYFQNIPDAYSIGTIIRHMDDFQRYYPRLYEKANHHLDRIWD 120 +I+++ L I EK++ +P + + +R + + +RY+P + K ++ W+ Sbjct: 62 RFREIIDNSNLTIPEKLKGILLIVPKDFQ-HSFVRLLIELRRYHPEQWAKIEQGINEEWE 120 Query: 121 GVIELVEEGMNRGELHKVDTVILKLMLNETLKKLLDYEFIAGHQVSFESGIKAMSDIVLY 180 + LVEEG+N G L +D +L L +K + + IA +Q++ + + M+DI+L+ Sbjct: 121 VITGLVEEGINSGVLRSMDIPVLIQTLKTLMKSIFEQGSIASNQITLQESLAMMADIILH 180 Query: 181 GL 182 G+ Sbjct: 181 GI 182 >C2XDL4.1 C2XDL4_BACCE Transcriptional regulator, TetR Length = 186 Score = 102 bits (254), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 53/182 (29%), Positives = 101/182 (55%), Gaps = 1/182 (0%) Query: 1 MDNQVYLKILKCFGAKGTRFTTEDLARELGTSKRTVYTYFSSKDEMIEKTIDFVFSQIIQ 60 M +++ + F +G +FT DLAR+LG SK T+Y +F SKDE+I ++ + + Sbjct: 2 MKDRIAKTAIALFHVRGIKFTMSDLARDLGVSKSTLYEHFPSKDELIGYVVEQINEEAQI 61 Query: 61 SDTDILEDEGLPIQEKIRLYFQNIPDAYSIGTIIRHMDDFQRYYPRLYEKANHHLDRIWD 120 +I+++ L I EK++ +P + + +R + + +RY+P + K ++ W+ Sbjct: 62 RFREIIDNSNLTIPEKLKGILLIVPKDFQ-HSFVRLLIELRRYHPEQWAKIEQGINEEWE 120 Query: 121 GVIELVEEGMNRGELHKVDTVILKLMLNETLKKLLDYEFIAGHQVSFESGIKAMSDIVLY 180 + LVEEG+N G L +D +L L +K + + IA +Q++ + + M+DI+L+ Sbjct: 121 VITGLVEEGINSGVLRSMDIPVLIQTLKTLMKSIFEQGSIASNQITLQESLAMMADIILH 180 Query: 181 GL 182 G+ Sbjct: 181 GI 182 >C2P0K2.1 C2P0K2_BACCE Transcriptional regulator, TetR Length = 186 Score = 102 bits (254), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 53/182 (29%), Positives = 101/182 (55%), Gaps = 1/182 (0%) Query: 1 MDNQVYLKILKCFGAKGTRFTTEDLARELGTSKRTVYTYFSSKDEMIEKTIDFVFSQIIQ 60 M +++ + F +G +FT DLAR+LG SK T+Y +F SKDE+I ++ + + Sbjct: 2 MKDRIAKTAIALFHVRGIKFTMSDLARDLGVSKSTLYEHFPSKDELIGYVVEQINEEAQI 61 Query: 61 SDTDILEDEGLPIQEKIRLYFQNIPDAYSIGTIIRHMDDFQRYYPRLYEKANHHLDRIWD 120 +I+++ L I EK++ +P + + +R + + +RY+P + K ++ W+ Sbjct: 62 RFREIIDNSNLTIPEKLKGILLIVPKDFQ-HSFVRLLIELRRYHPEQWAKIEQGINEEWE 120 Query: 121 GVIELVEEGMNRGELHKVDTVILKLMLNETLKKLLDYEFIAGHQVSFESGIKAMSDIVLY 180 + LVEEG+N G L +D +L L +K + + IA +Q++ + + M+DI+L+ Sbjct: 121 VITGLVEEGINSGVLRSMDIPVLIQTLKTLMKSIFEQGSIASNQITLQESLAMMADIILH 180 Query: 181 GL 182 G+ Sbjct: 181 GI 182 >C0FR56.1 C0FR56_9FIRM Putative uncharacterized protein Length = 185 Score = 102 bits (254), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 49/175 (28%), Positives = 97/175 (55%), Gaps = 1/175 (0%) Query: 10 LKCFGAKGTRFTTEDLARELGTSKRTVYTYFSSKDEMIEKTIDFVFSQIIQSDTDILEDE 69 ++ F KG +FT +D+A LG SK+T+YT F K+ ++ + +D+ F I +S+ +L D Sbjct: 12 IQAFNKKGLKFTMDDIAALLGISKKTIYTVFQDKNTLVSEMVDYCFDSIKESEQKVLSDT 71 Query: 70 GLPIQEKIRLYFQNIPDAYSIGTIIRHMDDFQRYYPRLYEKANHHLDRIWDGVIELVEEG 129 L KIR +P+ Y R + + YP++Y+K L+ W+ I L+++G Sbjct: 72 SLDTVGKIRAILGVLPEGYK-DIDFRQLYLLKDKYPKIYKKVEQRLETGWETTIALIQQG 130 Query: 130 MNRGELHKVDTVILKLMLNETLKKLLDYEFIAGHQVSFESGIKAMSDIVLYGLIK 184 ++ G + + ILK M+ TL++ + + +Q+S+ ++ + +I++ G+ + Sbjct: 131 IHEGTVRPIQIPILKTMMEATLEQFFQRDILITNQISYNEALEEVVNILVDGITR 185 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.322 0.140 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,073,034,347 Number of extensions: 43190173 Number of successful extensions: 130380 Number of sequences better than 1.0e-05: 133 Number of HSP's gapped: 130591 Number of HSP's successfully gapped: 133 Length of query: 188 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 60 Effective length of database: 1,854,806,283 Effective search space: 111288376980 Effective search space used: 111288376980 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)