BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0051.p (304 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KH26.1 B3KH26_9BACI LysR substrate-binding 438 e-121 B1DE07.1 B1DE07_9BACL Transcriptional regulator, LysR family 334 6e-90 P37499.2 YYBE_BACSU Uncharacterized HTH-type transcriptional reg... 213 2e-53 C0Z977.1 C0Z977_BREBN Transcriptional regulator 209 3e-52 A7ZAS3.1 A7ZAS3_BACA2 YybE 204 9e-51 >B3KH26.1 B3KH26_9BACI LysR substrate-binding Length = 295 Score = 438 bits (1127), Expect = e-121, Method: Compositional matrix adjust. Identities = 207/293 (70%), Positives = 249/293 (84%), Gaps = 1/293 (0%) Query: 6 LELTQLEYFITVAKLEHMTKAAKALNLTQPALSHAISKLEDELGLPLFDRGGRNIRLNRY 65 +ELTQLEYF+TV++L+HMTKA+KAL++TQPALSHAISKLE ELG+PLF+R GRN++LNRY Sbjct: 1 MELTQLEYFLTVSRLQHMTKASKALSITQPALSHAISKLESELGVPLFERNGRNVKLNRY 60 Query: 66 GVQFAKRVEEALRSIEGGMQEIREWSDPDTGLISISYLNILGVDLVPNLVQKYMQEHPKV 125 G FA+ VE+AL+ I+ GMQEI E S+PDTG+IS+SYLNILGVDLVP LV+ Y HPKV Sbjct: 61 GSMFARWVEQALQDIQNGMQEIEESSNPDTGIISVSYLNILGVDLVPALVRAYQLAHPKV 120 Query: 126 RFELFQGNLGDLDEHFELGSSDLIITSRESTVMKHEWLIIQKVPLYIVVPARHPFARQSS 185 RFEL QGNLGD+DEH E G SDL+ITSREST+ +H WL+++ +PLYIVV + H A + S Sbjct: 121 RFELHQGNLGDIDEHLEKGHSDLMITSRESTIDQHNWLVMRTMPLYIVVSSHHRLANRPS 180 Query: 186 LSLTKLSGEPFVGLKKNCGLKATITARFENTGFQLASTYDAEDLVTVAGFIKAGLGVSVL 245 LSL LSGEPFVGLK NCGL+ATI +RF++TGF L+STYDAEDL TVAGFIK GLGVSVL Sbjct: 181 LSLMDLSGEPFVGLKHNCGLRATIQSRFQHTGFILSSTYDAEDLATVAGFIKEGLGVSVL 240 Query: 246 PQTPGLMLEGLAWIPIAEEGWVWEIGLKWRKDRYLPAAAKAFINFIE-QLQVS 297 P+T GLMLEGL+WIPI +EGW WEIGLKWR+DRYL AA+ FI ++E QVS Sbjct: 241 PKTAGLMLEGLSWIPITDEGWFWEIGLKWREDRYLSPAARRFIEYVEHHYQVS 293 >B1DE07.1 B1DE07_9BACL Transcriptional regulator, LysR family Length = 292 Score = 334 bits (857), Expect = 6e-90, Method: Compositional matrix adjust. Identities = 155/288 (53%), Positives = 218/288 (75%) Query: 6 LELTQLEYFITVAKLEHMTKAAKALNLTQPALSHAISKLEDELGLPLFDRGGRNIRLNRY 65 +ELTQLEYF+ VA+ EH+T+AA++L++TQPALSH+I KLE ELG+PLF+R GR++++NRY Sbjct: 1 MELTQLEYFLRVARTEHLTRAAESLSITQPALSHSILKLEAELGVPLFERKGRSLKINRY 60 Query: 66 GVQFAKRVEEALRSIEGGMQEIREWSDPDTGLISISYLNILGVDLVPNLVQKYMQEHPKV 125 G+ F+K VE L+ +E G +EI E S+P++G+I ++YLNILGVDL+P L+++Y +P + Sbjct: 61 GMMFSKHVERILQEVERGKKEIEEHSNPESGVIHLAYLNILGVDLIPKLIREYQLINPAI 120 Query: 126 RFELFQGNLGDLDEHFELGSSDLIITSRESTVMKHEWLIIQKVPLYIVVPARHPFARQSS 185 FEL QG+ G + E G +DL++TS + +EW+ + +PLY+VV H A Q S Sbjct: 121 TFELSQGDKGAILNQVEEGHADLMVTSEQPVNDTYEWVPMLSLPLYLVVSKEHRLADQES 180 Query: 186 LSLTKLSGEPFVGLKKNCGLKATITARFENTGFQLASTYDAEDLVTVAGFIKAGLGVSVL 245 + L +++GEPFVGLK NC LK ++ AR ++ F LASTYDAEDL TVAGF+ AGLG+SVL Sbjct: 181 IHLGEITGEPFVGLKHNCSLKNSLLARVHHSNFTLASTYDAEDLQTVAGFVAAGLGISVL 240 Query: 246 PQTPGLMLEGLAWIPIAEEGWVWEIGLKWRKDRYLPAAAKAFINFIEQ 293 P+T GL LEG+ WIPIAEEGW WE+GL +K+R+L A F+ FI++ Sbjct: 241 PRTAGLNLEGIKWIPIAEEGWRWEVGLHLKKERFLSPATARFVRFIKE 288 >P37499.2 YYBE_BACSU Uncharacterized HTH-type transcriptional regulator yybE Length = 292 Score = 213 bits (542), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 109/286 (38%), Positives = 174/286 (60%) Query: 6 LELTQLEYFITVAKLEHMTKAAKALNLTQPALSHAISKLEDELGLPLFDRGGRNIRLNRY 65 +E QLEYF T+A+++H+TKAAK+L++TQPALS +I++LE+ LG+PLFDR GR+I LN+Y Sbjct: 1 MEWEQLEYFQTLARMQHVTKAAKSLSITQPALSRSIARLENHLGVPLFDRQGRSISLNQY 60 Query: 66 GVQFAKRVEEALRSIEGGMQEIREWSDPDTGLISISYLNILGVDLVPNLVQKYMQEHPKV 125 G F +RV+ ++ G +EI+ PD G++S+ +L+ LG LVP+L+ + QE+P + Sbjct: 61 GHIFLRRVQAMMKEYTEGKEEIQALLKPDQGVVSLGFLHTLGTTLVPDLIGSFQQEYPNI 120 Query: 126 RFELFQGNLGDLDEHFELGSSDLIITSRESTVMKHEWLIIQKVPLYIVVPARHPFARQSS 185 F+L Q + L E + G DL + + +W+ + L++ VP HP A + S Sbjct: 121 SFQLKQNHSYWLLERLKSGDLDLCLLASIKPENPIQWIKLWSEELFVFVPNDHPLASRES 180 Query: 186 LSLTKLSGEPFVGLKKNCGLKATITARFENTGFQLASTYDAEDLVTVAGFIKAGLGVSVL 245 ++L +++GE F+ LKK L+ T+ FE Q ++ E+ T AGF+ AGLG+S+L Sbjct: 181 ITLNEIAGERFILLKKGYALRMTVDELFEKANIQPNIMFEGEEATTAAGFVAAGLGISIL 240 Query: 246 PQTPGLMLEGLAWIPIAEEGWVWEIGLKWRKDRYLPAAAKAFINFI 291 P GL + I ++ IG+ W K R+L A+ F ++ Sbjct: 241 PDLKGLDQSKITKIRVSWPECQRVIGIAWIKGRFLSPVAETFKQYV 286 >C0Z977.1 C0Z977_BREBN Transcriptional regulator Length = 294 Score = 209 bits (531), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 104/286 (36%), Positives = 166/286 (58%) Query: 6 LELTQLEYFITVAKLEHMTKAAKALNLTQPALSHAISKLEDELGLPLFDRGGRNIRLNRY 65 +E Q EYF T+A+++H+T AA+AL+++QPALS +I++ EDE+G+PLFDR GR+IRLN+Y Sbjct: 1 MEWQQFEYFQTLARIQHVTHAAEALSISQPALSRSIARFEDEIGVPLFDRQGRSIRLNKY 60 Query: 66 GVQFAKRVEEALRSIEGGMQEIREWSDPDTGLISISYLNILGVDLVPNLVQKYMQEHPKV 125 G F KRVE ++ G QEI + DPD G +S+ +L+ L +P+L+ + +P + Sbjct: 61 GQLFLKRVENMMKEFHEGKQEIMDLLDPDKGEVSLGFLHTLSTSRIPDLLASFRSHYPNI 120 Query: 126 RFELFQGNLGDLDEHFELGSSDLIITSRESTVMKHEWLIIQKVPLYIVVPARHPFARQSS 185 F L QG L + G DL + + W + L+++VP H +A ++S Sbjct: 121 HFRLGQGPSHTLMNQLQGGEFDLCLLAPMDMKSPFVWKPLWDEELFVIVPKDHKYANRTS 180 Query: 186 LSLTKLSGEPFVGLKKNCGLKATITARFENTGFQLASTYDAEDLVTVAGFIKAGLGVSVL 245 ++L +++ E F+ LK+ L+ T+ F+ G T++ E+ TVAG + AGLG+S+L Sbjct: 181 ITLKEIADESFIHLKEGYSLRITVEQLFQEAGITPPITFEGEEADTVAGLVAAGLGISLL 240 Query: 246 PQTPGLMLEGLAWIPIAEEGWVWEIGLKWRKDRYLPAAAKAFINFI 291 P G + IP+ IG+ W + RYL A + F F+ Sbjct: 241 PNLKGTDQSKITQIPVKSPKCQRTIGIAWVEGRYLSPATEKFQQFV 286 >A7ZAS3.1 A7ZAS3_BACA2 YybE Length = 296 Score = 204 bits (519), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 113/286 (39%), Positives = 164/286 (57%), Gaps = 6/286 (2%) Query: 10 QLEYFITVAKLEHMTKAAKALNLTQPALSHAISKLEDELGLPLFDRGGRNIRLNRYGVQF 69 Q+ YF TVA+++H+T+AAK L+++QPALS +ISKLEDELG+ LFDR GRNI LNRYG F Sbjct: 5 QINYFQTVAQVQHITQAAKQLSISQPALSRSISKLEDELGVQLFDRKGRNIYLNRYGKMF 64 Query: 70 AKRVEEALRSIEGGMQEIREWSDPDTGLISISYLNILGVDLVPNLVQKYMQEHPKVRFEL 129 RVE+++R IE G QE+ P++G I +S+L LG+ +VP+++ + +P V F+L Sbjct: 65 LHRVEQSIRQIEIGKQEVWNEIHPNSGTILLSFLPSLGMSMVPDVISSFQHMYPHVNFQL 124 Query: 130 FQGNLGDLDEHFELGSSDLIITSRESTVMKHEWLIIQKV---PLYIVVPARHPFARQSSL 186 Q + + E D+ +TS +++ +I Q + LY+ V A HP A + Sbjct: 125 TQASNQQIIEQLTSREVDIALTSLRD---ENDDVICQPLLTEELYLAVSAEHPLASYDEI 181 Query: 187 SLTKLSGEPFVGLKKNCGLKATITARFENTGFQLASTYDAEDLVTVAGFIKAGLGVSVLP 246 L EPF+ K L I E GF ++ ED+VT +G + A LGVS++P Sbjct: 182 DLKMAEHEPFISFKDTNVLHGMIKELCEKAGFSPDVVFEGEDIVTASGLVGAKLGVSLIP 241 Query: 247 QTPGLMLEGLAWIPIAEEGWVWEIGLKWRKDRYLPAAAKAFINFIE 292 + + + EIGL WRKD YL AA+ FI FI+ Sbjct: 242 DLHVFDKTKVKLLSVTNPICEREIGLAWRKDGYLSPAAENFIAFIQ 287 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,799,653,674 Number of extensions: 74906507 Number of successful extensions: 220942 Number of sequences better than 1.0e-05: 15255 Number of HSP's gapped: 210968 Number of HSP's successfully gapped: 15259 Length of query: 304 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 169 Effective length of database: 1,788,859,178 Effective search space: 302317201082 Effective search space used: 302317201082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)