BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0049.p (278 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KH24.1 B3KH24_9BACI Short-chain dehydrogenase/reductase SDR 322 2e-86 B1D5G8.1 B1D5G8_9BACL Short-chain dehydrogenase/reductase SDR 271 3e-71 B9XHR3.1 B9XHR3_9BACT Short-chain dehydrogenase/reductase SDR 240 9e-62 A8L3Q6.1 A8L3Q6_FRASN Short-chain dehydrogenase/reductase SDR 231 5e-59 A9F422.1 A9F422_SORC5 Putative 3-oxoacyl-[acyl-carrier-protein] ... 228 6e-58 >B3KH24.1 B3KH24_9BACI Short-chain dehydrogenase/reductase SDR Length = 264 Score = 322 bits (825), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 171/264 (64%), Positives = 195/264 (73%) Query: 15 MSAVYKDPLHEFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTTARTLPADLQEPGLFVQ 74 M ++ P +EFEGKRVLVTGGTRGMG+AI KRL+ GA VLTTAR+ P+ GLFVQ Sbjct: 1 MKHRHEGPWNEFEGKRVLVTGGTRGMGQAIVKRLSDGGAAVLTTARSEPSVRVGSGLFVQ 60 Query: 75 ADIATPEGVEQVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXXV 134 ADIAT GV++VI+GV+E L GIDI+V+ VGGS+TPPGGAL LTDED V Sbjct: 61 ADIATSTGVDKVIQGVQEHLHGIDIVVHCVGGSTTPPGGALALTDEDWVQALNWNLLAAV 120 Query: 135 RLDRGLLPMMLEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRGI 194 RLDRGLLP M+EQG GAI+HI+SIQRRLPLYE GLSNEFSPRGI Sbjct: 121 RLDRGLLPYMVEQGSGAIIHITSIQRRLPLYETTLAYAAAKAALANYSKGLSNEFSPRGI 180 Query: 195 RINTVAPGFIQTQAAEAMIDRIVQAAGSREAALQQLMDSXXXXXXXXXXXXEEVGELVAF 254 RIN V+PGFIQT+AA+AMIDRI +GSRE+ALQ+LMDS EEV ELVAF Sbjct: 181 RINAVSPGFIQTEAADAMIDRIASVSGSRESALQELMDSLGGIPLGRPGLPEEVAELVAF 240 Query: 255 LVSDRAASITGSEYVIDGGTIPTV 278 LVSDRA+SITGSEYVIDGGTIPTV Sbjct: 241 LVSDRASSITGSEYVIDGGTIPTV 264 >B1D5G8.1 B1D5G8_9BACL Short-chain dehydrogenase/reductase SDR Length = 263 Score = 271 bits (694), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 154/255 (60%), Positives = 175/255 (68%), Gaps = 1/255 (0%) Query: 25 EFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTTARTLPADLQEPGL-FVQADIATPEGV 83 EF+GKR LVTGGT+GMG AI KRLA +GATVLTTAR +P++ P + FVQADI PEG Sbjct: 9 EFQGKRALVTGGTQGMGLAIVKRLADAGATVLTTARNIPSEYHIPNVHFVQADITKPEGT 68 Query: 84 EQVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXXVRLDRGLLPM 143 + + VKE LGGIDIL+N VGGS +P GGAL LTDE +RLDR LLP+ Sbjct: 69 KAIFDAVKEKLGGIDILINTVGGSGSPAGGALALTDEHWLEELNMNLLGSIRLDRSLLPI 128 Query: 144 MLEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRGIRINTVAPGF 203 ML QG G I+H+SSIQR LPLYE GLSNEF+PRGIR+N +APGF Sbjct: 129 MLGQGKGVIIHVSSIQRTLPLYESTLAYAAAKAALATYSKGLSNEFAPRGIRVNAIAPGF 188 Query: 204 IQTQAAEAMIDRIVQAAGSREAALQQLMDSXXXXXXXXXXXXEEVGELVAFLVSDRAASI 263 IQT+AA +IDR+ AGSREAAL QLMDS EEV EL AFL SDRAASI Sbjct: 189 IQTEAANRLIDRLAVTAGSREAALNQLMDSLGGIPFGRPGLPEEVAELAAFLASDRAASI 248 Query: 264 TGSEYVIDGGTIPTV 278 GSEYVIDGGTIPTV Sbjct: 249 HGSEYVIDGGTIPTV 263 >B9XHR3.1 B9XHR3_9BACT Short-chain dehydrogenase/reductase SDR Length = 265 Score = 240 bits (613), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 134/266 (50%), Positives = 166/266 (62%), Gaps = 3/266 (1%) Query: 15 MSAVYKDPLH-EFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTTARTLPADLQEPGLFV 73 M+A P EF+GKRVLVTGGT+G G+AIA R GATV+ TAR+ P + + F+ Sbjct: 1 MNAAKNTPYESEFDGKRVLVTGGTKGAGKAIADRFQRGGATVIITARSAPEEKTD-NHFI 59 Query: 74 QADIATPEGVEQVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXX 133 Q D++ PEG +VI + GIDI+V+NVGGSS P GG + +TDE Sbjct: 60 QTDVSNPEGTSKVINEILNRFHGIDIIVHNVGGSSAPSGGFITVTDEIWQQNINENLYPA 119 Query: 134 VRLDRGLLPMMLEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRG 193 VRLDRGLLP M+E+G G I+HISSIQR LPLY+ LSNE SP+G Sbjct: 120 VRLDRGLLPSMIERGSGVIIHISSIQRALPLYDSTIAYAAAKAALSNYSKALSNEVSPKG 179 Query: 194 IRINTVAPGFIQTQAAEAMIDRIVQAAGSREA-ALQQLMDSXXXXXXXXXXXXEEVGELV 252 IR+ TV+PGFI+T AA MIDR+ + S A A Q+LMD EV ELV Sbjct: 180 IRVVTVSPGFIETDAATRMIDRMAEKDKSDYATARQKLMDMLGGIPLGRPNSPHEVAELV 239 Query: 253 AFLVSDRAASITGSEYVIDGGTIPTV 278 AF+ SDRA+SITG+E+VIDGGTIPTV Sbjct: 240 AFVASDRASSITGTEFVIDGGTIPTV 265 >A8L3Q6.1 A8L3Q6_FRASN Short-chain dehydrogenase/reductase SDR Length = 261 Score = 231 bits (589), Expect = 5e-59, Method: Compositional matrix adjust. Identities = 124/255 (48%), Positives = 159/255 (62%), Gaps = 1/255 (0%) Query: 25 EFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTTARTLPADLQEPGLFVQADIATPEGVE 84 +G+ LVTG ++G G AIA RL +GA VL TART P+ P LF+ AD+ T EGV Sbjct: 6 RLDGRPALVTGASKGTGAAIADRLREAGAIVLATARTRPSGQAGPDLFIPADLTTTEGVA 65 Query: 85 QVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXXVRLDRGLLPMM 144 V+ V+ G +DI+V+ +GGS PPGG LTD+ VRLDR LLP M Sbjct: 66 AVVDQVRNRFGTLDIVVHTLGGSQAPPGGFAALTDQHWDHELRLNLLAAVRLDRALLPAM 125 Query: 145 LEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRGIRINTVAPGFI 204 ++ G GA+VH+SSIQRR+PLYE GL+NE PRG+R+N V+PGFI Sbjct: 126 IDAGGGAVVHVSSIQRRMPLYEATLGYAAAKAALTTYSKGLANEVGPRGVRVNVVSPGFI 185 Query: 205 QTQAAEAMIDRIVQA-AGSREAALQQLMDSXXXXXXXXXXXXEEVGELVAFLVSDRAASI 263 QT A+ +IDRI + A SR AL+Q+MDS EV ELVAFLVSDRAA+I Sbjct: 186 QTAGADGLIDRIAASQAISRGQALEQVMDSLGGIPLGRPAQPTEVAELVAFLVSDRAAAI 245 Query: 264 TGSEYVIDGGTIPTV 278 TG+E+ +DGGT+PT+ Sbjct: 246 TGAEHTVDGGTVPTI 260 >A9F422.1 A9F422_SORC5 Putative 3-oxoacyl-[acyl-carrier-protein] reductase EC=1.1.1.100 Length = 267 Score = 228 bits (580), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 134/260 (51%), Positives = 170/260 (65%), Gaps = 4/260 (1%) Query: 21 DPLHEFEGKRVLVTGGTRGMGEAIAKRLAASGATVLTTART-LPADLQEPGLFVQADIAT 79 DP EF GKRVLVTGGT+G GEAI +RL A+GA TTAR+ LPA Q P +FVQAD+AT Sbjct: 10 DP-EEFAGKRVLVTGGTKGAGEAIVRRLGAAGAVTATTARSALPAG-QSPNVFVQADLAT 67 Query: 80 PEGVEQVIRGVKEVLGGIDILVNNVGGSSTPPGGALVLTDEDXXXXXXXXXXXXVRLDRG 139 GV+ V+R V LGGIDILV++VGGSS P GG LT++ VRLDR Sbjct: 68 VAGVDAVVRAVLGELGGIDILVHSVGGSSAPGGGFASLTEDIWHDELSLNLMAAVRLDRA 127 Query: 140 LLPMMLEQGYGAIVHISSIQRRLPLYEXXXXXXXXXXXXXXXXXGLSNEFSPRGIRINTV 199 LLP M+ +G G ++H+SSIQRRLPL++ LS E P+G+R+N++ Sbjct: 128 LLPHMIARGAGVVLHVSSIQRRLPLHDATIAYASAKAALSTYSKALSKELGPKGVRVNSI 187 Query: 200 APGFIQTQAAEAMIDRIVQAAGSRE-AALQQLMDSXXXXXXXXXXXXEEVGELVAFLVSD 258 APG+I+T AA+A++ R+ AG+ E A Q +M++ EEV ELVAFL SD Sbjct: 188 APGWIRTTAADALVKRLADGAGTDEDTARQGIMNALGGIPIGRPAWPEEVAELVAFLASD 247 Query: 259 RAASITGSEYVIDGGTIPTV 278 RAASI GSEY+IDGGT+PTV Sbjct: 248 RAASIHGSEYLIDGGTVPTV 267 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.135 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,357,524,395 Number of extensions: 47394159 Number of successful extensions: 253555 Number of sequences better than 1.0e-05: 11221 Number of HSP's gapped: 246599 Number of HSP's successfully gapped: 11230 Length of query: 278 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 144 Effective length of database: 1,798,280,193 Effective search space: 258952347792 Effective search space used: 258952347792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)