BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0035.p (287 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value C4C0U1.1 C4C0U1_9FUSO Aldo/keto reductase, diketogulonate reductase 365 2e-99 B1YFS1.1 B1YFS1_EXIS2 Aldo/keto reductase 357 5e-97 C4L5S5.1 C4L5S5_EXISA Aldo/keto reductase 350 1e-94 Q4Q5X1.1 Q4Q5X1_LEIMA Aldehyde reductase, putative (Oxidoreducta... 344 5e-93 A4HJS6.1 A4HJS6_LEIBR Aldehyde reductase, putative (Oxidoreducta... 342 2e-92 >C4C0U1.1 C4C0U1_9FUSO Aldo/keto reductase, diketogulonate reductase Length = 294 Score = 365 bits (937), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 175/286 (61%), Positives = 217/286 (75%) Query: 2 NTNISGTITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDG 61 N N + L+DG+ V +GQGTW+MG+ + R +EI ALRLG++LGM LIDTAEMYG G Sbjct: 9 NENFQRNVELRDGSLVNAIGQGTWHMGEKHSERAKEIKALRLGIDLGMTLIDTAEMYGSG 68 Query: 62 RSEEMVGEAIRGIRDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKV 121 +EE+ GEA++GIRD++F+VSKV+PQNAG R SCE SLKRLGTD++DLYLLHWRG + Sbjct: 69 GAEEVTGEAVKGIRDKIFIVSKVYPQNAGKKRAVLSCENSLKRLGTDYIDLYLLHWRGGI 128 Query: 122 PLEETVKGMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHE 181 PL ETV+ MEQLV GKI RWGVSN DT DM+EL +IP G CAVNQVLYHLGSRGIE++ Sbjct: 129 PLHETVEVMEQLVKEGKIKRWGVSNFDTKDMEELWKIPDGSNCAVNQVLYHLGSRGIEYD 188 Query: 182 LLPWQRTHKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDII 241 LLPW R K+PVMAY P+AQ G LRK + N+TVR IA + G + +Q++LAW++RE DII Sbjct: 189 LLPWCREKKLPVMAYCPVAQGGILRKELLANKTVRRIAGERGISEVQVLLAWTVREKDII 248 Query: 242 AIPKASSRKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 +IPKA H+ ENAE A I LS EE L+ FP P KV LD++ Sbjct: 249 SIPKAVQENHIYENAETAGIFLSGEEIRDLELEFPAPDTKVWLDIV 294 >B1YFS1.1 B1YFS1_EXIS2 Aldo/keto reductase Length = 296 Score = 357 bits (917), Expect = 5e-97, Method: Compositional matrix adjust. Identities = 168/280 (60%), Positives = 213/280 (76%) Query: 8 TITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDGRSEEMV 67 T+TL D T V +GQGTW MG+ + R EI AL +G++ GM LIDTAEMYG+G SE+++ Sbjct: 17 TVTLPDQTHVRALGQGTWRMGEDQSKRQAEIEALHVGLDSGMELIDTAEMYGEGASEQLI 76 Query: 68 GEAIRGIRDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKVPLEETV 127 E ++ RD VFLVSKV+P NAG +++ +CE+SLK LGTD+LDLYLLHWRG +PL+ETV Sbjct: 77 REVLKERRDDVFLVSKVYPHNAGGEKLRAACEQSLKNLGTDYLDLYLLHWRGSIPLQETV 136 Query: 128 KGMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHELLPWQR 187 G+EQL A GKI RWGVSN D +DMKELL + GD+CAVNQVLYHLGSRGIE+EL+P + Sbjct: 137 DGLEQLKAEGKIRRWGVSNFDVSDMKELLSLRNGDQCAVNQVLYHLGSRGIEYELMPLLK 196 Query: 188 THKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDIIAIPKAS 247 H IPVMAY PLA+ G LR + ++ TV +AE+HG P QI+LAW+IR GD+IAIPKA Sbjct: 197 QHGIPVMAYCPLAEGGSLRDQLLDHPTVERLAEEHGVKPAQILLAWAIRSGDVIAIPKAG 256 Query: 248 SRKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 KHV EN +AA + L++EE LD+AFP P K PLD++ Sbjct: 257 QAKHVEENGKAALVELTDEEYAALDEAFPAPTKKEPLDIV 296 >C4L5S5.1 C4L5S5_EXISA Aldo/keto reductase Length = 297 Score = 350 bits (897), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 165/280 (58%), Positives = 211/280 (75%) Query: 8 TITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDGRSEEMV 67 + LKDG + +GQGTW MG+ + D E+ ALR G++ G+++IDTAEMYG+G SE++V Sbjct: 18 VVELKDGREMRALGQGTWRMGEDKSKHDAEVEALRTGLDCGLHVIDTAEMYGEGESEKLV 77 Query: 68 GEAIRGIRDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKVPLEETV 127 GEAI+ R+ VFLVSKV+P NA D++ +C+ SL+RLGTD+LDLYLLHWRG PLEET+ Sbjct: 78 GEAIQDRREDVFLVSKVYPYNAYGDKLVSACDASLERLGTDYLDLYLLHWRGDAPLEETI 137 Query: 128 KGMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHELLPWQR 187 +GMEQL A GKI WGVSN D DM+ELL +P GD+CAVNQVLYHLGSRGIE++LLP+ Sbjct: 138 QGMEQLKADGKIRAWGVSNFDVDDMEELLSLPNGDKCAVNQVLYHLGSRGIEYDLLPYLE 197 Query: 188 THKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDIIAIPKAS 247 IPVMAY+PLA+ ++K V ENETV IAE + A+ QI+LAW+IR G +IAIPKA Sbjct: 198 EQGIPVMAYAPLAEGSDVKKDVFENETVNRIAEAYSASVAQILLAWTIRSGHVIAIPKAG 257 Query: 248 SRKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 +HV ENA A + +SEE+ LD+AFP P K PLD+I Sbjct: 258 QAEHVEENAAALLLDMSEEDFAALDEAFPPPTTKQPLDVI 297 >Q4Q5X1.1 Q4Q5X1_LEIMA Aldehyde reductase, putative (Oxidoreductase, putative) Length = 286 Score = 344 bits (883), Expect = 5e-93, Method: Compositional matrix adjust. Identities = 164/281 (58%), Positives = 209/281 (74%), Gaps = 1/281 (0%) Query: 8 TITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDGRSEEMV 67 + L DGTA +GQG W MG++ R E+AALR G+E GM LIDTAEMYG+GRSE +V Sbjct: 6 VVRLPDGTAAPALGQGVWMMGETPENRTRELAALRAGMEAGMTLIDTAEMYGNGRSERLV 65 Query: 68 GEAIRGI-RDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKVPLEET 126 EAI+ R+++F+VSKV P NA I RSC+ SL+ +GTD+LDLYLLHWRG+VPL ET Sbjct: 66 AEAIKETPRERLFIVSKVLPTNASRAAIFRSCDASLQNIGTDYLDLYLLHWRGRVPLSET 125 Query: 127 VKGMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHELLPWQ 186 V ME+LV SGKI RWGVSN D DMKEL R+PGGD+CAVNQVLYHLGSRGIE++LLPW Sbjct: 126 VTCMEELVKSGKIRRWGVSNFDVDDMKELWRVPGGDKCAVNQVLYHLGSRGIEYDLLPWL 185 Query: 187 RTHKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDIIAIPKA 246 R HK+PVMAY P+AQAG+L+ + ++ VR +A +H AT Q++LA+ +R G +IAIP++ Sbjct: 186 REHKVPVMAYCPIAQAGELKSELYKSTVVRSVAARHNATVTQVLLAFVLRSGHVIAIPRS 245 Query: 247 SSRKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 S+ H ENA A I L+E + +LD AFP P K LD++ Sbjct: 246 SNPAHTEENAAADSIELTEADMAQLDAAFPAPKRKTHLDIV 286 >A4HJS6.1 A4HJS6_LEIBR Aldehyde reductase, putative (Oxidoreductase, putative) Length = 286 Score = 342 bits (878), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 161/281 (57%), Positives = 207/281 (73%), Gaps = 1/281 (0%) Query: 8 TITLKDGTAVTKMGQGTWNMGDSTATRDEEIAALRLGVELGMNLIDTAEMYGDGRSEEMV 67 + L DGT +GQG W MGD+ A R E+AALR GV GM LIDTAEMYG+GRSE +V Sbjct: 6 VVRLPDGTTAPALGQGVWMMGDNPAKRAVELAALRAGVAAGMTLIDTAEMYGNGRSERLV 65 Query: 68 GEAIRGI-RDQVFLVSKVHPQNAGLDRITRSCEESLKRLGTDHLDLYLLHWRGKVPLEET 126 EAI+ R+ +F+VSKV PQNA I R C+ SL+ +GTD+LDLYLLHWRG VPL ET Sbjct: 66 AEAIKETPRESLFVVSKVLPQNASRANIFRCCDASLQNIGTDYLDLYLLHWRGAVPLSET 125 Query: 127 VKGMEQLVASGKIARWGVSNLDTADMKELLRIPGGDRCAVNQVLYHLGSRGIEHELLPWQ 186 V +E+LV SGKI RWGVSN D DM+EL R+PGGD+CAVNQVLYHLGSRGIE++L+PW Sbjct: 126 VTCLEELVKSGKIRRWGVSNFDVNDMEELWRVPGGDKCAVNQVLYHLGSRGIEYDLIPWL 185 Query: 187 RTHKIPVMAYSPLAQAGKLRKGVTENETVREIAEKHGATPLQIMLAWSIREGDIIAIPKA 246 H +PVMAY P+AQAG+L+ + ++ V+ +A +H AT Q++LA+ +R G +IAIP++ Sbjct: 186 TAHNVPVMAYCPIAQAGELKAELYKSAVVQSVAARHSATVAQVLLAFVLRSGHVIAIPRS 245 Query: 247 SSRKHVTENAEAAKIRLSEEECWKLDDAFPQPAWKVPLDMI 287 S+ H ENAEA I L+EE+ +L+ AFP P K PLD++ Sbjct: 246 SNPAHTKENAEAGGIELTEEDMVELNAAFPAPKCKTPLDIV 286 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,729,972,704 Number of extensions: 71812854 Number of successful extensions: 214765 Number of sequences better than 1.0e-05: 8838 Number of HSP's gapped: 196044 Number of HSP's successfully gapped: 8880 Length of query: 287 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 153 Effective length of database: 1,798,280,193 Effective search space: 275136869529 Effective search space used: 275136869529 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)