BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0031.p (487 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8D4.1 B3K8D4_9BACI PTS system, mannitol-specific IIC subunit 691 0.0 B1DC75.1 B1DC75_9BACL PTS system, mannitol-specific IIC subunit ... 666 0.0 C0Z8I9.1 C0Z8I9_BREBN PTS system IIBC component EC=2.7.1.69 615 e-174 B7GJR8.1 B7GJR8_ANOFW Phosphotransferase system (PTS) mannitol-s... 597 e-169 P50852.1 PTMCB_BACST PTS system mannitol-specific EIICB componen... 591 e-167 >B3K8D4.1 B3K8D4_9BACI PTS system, mannitol-specific IIC subunit Length = 477 Score = 691 bits (1784), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/476 (73%), Positives = 392/476 (82%), Gaps = 14/476 (2%) Query: 14 VRVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLI 73 +RV+VQQ GR+LSGMVMPNIGAFIAWGLITALFIPTGW PN+ L LVDPMIK+LLPLLI Sbjct: 13 IRVRVQQLGRLLSGMVMPNIGAFIAWGLITALFIPTGWFPNEKLGALVDPMIKYLLPLLI 72 Query: 74 GYTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKA 133 GYTGGQMI+GKRG VIGA+AT+GVIVGA IPMFLGAMIVGPLA WVL+ FD+ ++GKIK+ Sbjct: 73 GYTGGQMIHGKRGAVIGAVATVGVIVGASIPMFLGAMIVGPLAAWVLKQFDKLIDGKIKS 132 Query: 134 GFEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPA 193 GFEMLVNNFS AY GIGPLVQG+T +LS GV+ LV+ NLLPLVNIIIEPA Sbjct: 133 GFEMLVNNFSLGIIGGALTLLAYTGIGPLVQGITKVLSAGVDVLVDANLLPLVNIIIEPA 192 Query: 194 KVLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAP 253 KVLFLNNAINHGI SP+A EESARIG+S+L+MLESNPGPGLGILLA+W G+GSA+SSAP Sbjct: 193 KVLFLNNAINHGIFSPLATEESARIGQSVLYMLESNPGPGLGILLAFWFFGRGSARSSAP 252 Query: 254 GAVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYL 313 GAV+IHFLGGIHEIYFPYILM P LILA I GGV+GTFTFQ+LGAGL SPGSI AY Sbjct: 253 GAVVIHFLGGIHEIYFPYILMKPVLILAAIAGGVAGTFTFQILGAGLVGPASPGSILAYF 312 Query: 314 AMTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSKNNEDDVDLEEASAKMREMKXXXXXX 373 AM PKGG P+ AGV+ ATVVSF KT K E++ +LEEASAKM+EMK Sbjct: 313 AMAPKGGLVPVLAGVLAATVVSFVIAALLLKT-GKQTEEEPNLEEASAKMKEMK------ 365 Query: 374 XXXXXXXXXNVITPNVTANVRN--KADVNKIVFACDAGMGSSAMGASVLRKKLQNAGVNI 431 + + P +A V N K +VNKIVFACDAGMGSSAMGASVLRKKLQ AGVNI Sbjct: 366 ----TGGMNDQVAP-ASAAVANLDKDNVNKIVFACDAGMGSSAMGASVLRKKLQQAGVNI 420 Query: 432 TVVNSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERLK 487 TVVNSAVSEIP DADIV+TQKTLT+RAIA NPNAEHISIDNFLKSP+YDELVERLK Sbjct: 421 TVVNSAVSEIPDDADIVITQKTLTERAIAKNPNAEHISIDNFLKSPRYDELVERLK 476 >B1DC75.1 B1DC75_9BACL PTS system, mannitol-specific IIC subunit EC=2.7.1.69 Length = 488 Score = 666 bits (1718), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/475 (68%), Positives = 381/475 (80%), Gaps = 2/475 (0%) Query: 14 VRVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLI 73 +RV VQ+FGR LSGMVMPNIGAFIAWG+ITALFIPTGWLPN+ LA LVDP+IK+LLPLLI Sbjct: 13 IRVGVQKFGRFLSGMVMPNIGAFIAWGIITALFIPTGWLPNETLAKLVDPIIKYLLPLLI 72 Query: 74 GYTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKA 133 GYTGG MI+ +RGGVIGA+ TMG+IVG DIPMFLGAMI GPLA WVL+ FD+++EGKIK+ Sbjct: 73 GYTGGTMIHKQRGGVIGALMTMGIIVGTDIPMFLGAMICGPLAAWVLKQFDKSIEGKIKS 132 Query: 134 GFEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPA 193 GFEMLVNNFS +Y G+GP+++ LT ILSNGVEFLVNHNL+PL NIIIEPA Sbjct: 133 GFEMLVNNFSLGIIGAILTLISYSGVGPVIEQLTKILSNGVEFLVNHNLIPLANIIIEPA 192 Query: 194 KVLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAP 253 K+LFLNNAINHG+LSPIA EE++R G+SILFMLESNPGPGLGILLAY + G+GSAK SA Sbjct: 193 KILFLNNAINHGVLSPIALEEASRTGQSILFMLESNPGPGLGILLAYMIAGRGSAKQSAS 252 Query: 254 GAVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYL 313 GA IIHF GGIHEIYFPYILMNPRLILAVI GGV+GTFTF L GAGL + PSPGSIFAY+ Sbjct: 253 GAAIIHFFGGIHEIYFPYILMNPRLILAVIAGGVTGTFTFSLFGAGLVAPPSPGSIFAYI 312 Query: 314 AMTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSKNNEDDVDLEEASAKMREMKXXXXXX 373 AM PKGG +G++ A +VSF KT+ K ED DLE+ASA+M+EMK Sbjct: 313 AMAPKGGLLAALSGIVTAAIVSFVIAALLLKTTKKEAEDGDDLEQASARMKEMKAAGNTA 372 Query: 374 XXXXXXXXXNVITPNVTAN--VRNKADVNKIVFACDAGMGSSAMGASVLRKKLQNAGVNI 431 + A+ V+ K DV+KIVF+CDAGMGSSAMGAS+LRKK++ AG ++ Sbjct: 373 GAATAVAAPQAVEHAEAASDTVKAKEDVHKIVFSCDAGMGSSAMGASILRKKMKAAGSDV 432 Query: 432 TVVNSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERL 486 TV+N+A+S+IP DADIV+T KTLTDRA P AEHISIDNFLKSP+YD+LV+RL Sbjct: 433 TVINTAISDIPGDADIVITHKTLTDRAKIQAPKAEHISIDNFLKSPEYDKLVDRL 487 >C0Z8I9.1 C0Z8I9_BREBN PTS system IIBC component EC=2.7.1.69 Length = 476 Score = 615 bits (1587), Expect = e-174, Method: Compositional matrix adjust. Identities = 307/474 (64%), Positives = 373/474 (78%), Gaps = 12/474 (2%) Query: 14 VRVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLI 73 +RV VQ+FGR LS MVMPNIGAFIAWGLITALFIPTGW PN++LA LV PMI +LLPLLI Sbjct: 13 IRVSVQKFGRFLSAMVMPNIGAFIAWGLITALFIPTGWRPNEELAKLVGPMITYLLPLLI 72 Query: 74 GYTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKA 133 GYTGG+M++ RGGV+GA+ATMGV+VGADIPMFLGAMI+GPL GWV++ D+ EGKI++ Sbjct: 73 GYTGGKMVHDVRGGVVGAVATMGVVVGADIPMFLGAMIMGPLGGWVIKKVDQLFEGKIRS 132 Query: 134 GFEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPA 193 GFEMLVNNFS A+K IGP+V GL+ L+ GVE +V LLPL +I IEPA Sbjct: 133 GFEMLVNNFSAGIVGGLLTLLAFKAIGPVVVGLSKALAAGVEVIVGAGLLPLASIFIEPA 192 Query: 194 KVLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAP 253 K+LFLNNAINHGILSPI +E+A+IG+S+ F+LESNPGPGLGILLAYWL G+GSAK SAP Sbjct: 193 KILFLNNAINHGILSPIGLDEAAKIGQSVFFLLESNPGPGLGILLAYWLAGRGSAKQSAP 252 Query: 254 GAVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYL 313 GAVIIHF GGIHEIYFPY+LMNPRLILA IGGG++G FTF LLGAGL + PSPGSIFA + Sbjct: 253 GAVIIHFFGGIHEIYFPYVLMNPRLILAAIGGGMAGVFTFTLLGAGLVAPPSPGSIFALI 312 Query: 314 AMTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSKNNEDDVDLEEASAKMREMKXXXXXX 373 AM P+GG P+ AGV+ ATVVSF K S+K +ED +LE+A+ KM+EMK Sbjct: 313 AMAPRGGLLPVLAGVLAATVVSFLIASALLKMSAKTDED--ELEKATEKMQEMK------ 364 Query: 374 XXXXXXXXXNVITPNVTANVRNKADVNKIVFACDAGMGSSAMGASVLRKKLQNAGV-NIT 432 V+ V V ADV K+VF+CDAGMGSSAMGA+ LRKK ++AG+ IT Sbjct: 365 --GKKAQEQEVVEAPVKHEVA-AADVKKVVFSCDAGMGSSAMGAASLRKKFKDAGLAEIT 421 Query: 433 VVNSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERL 486 V+N+A+++IP+DADIV+T K+LT+RA P+AEHISI+NFL SP+YD+LV+RL Sbjct: 422 VINTAINDIPSDADIVITHKSLTERAQVKAPSAEHISIENFLNSPEYDKLVKRL 475 >B7GJR8.1 B7GJR8_ANOFW Phosphotransferase system (PTS) mannitol-specific IIBC component Length = 466 Score = 597 bits (1540), Expect = e-169, Method: Compositional matrix adjust. Identities = 304/473 (64%), Positives = 359/473 (75%), Gaps = 17/473 (3%) Query: 15 RVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLIG 74 RVKVQ+FG LSGM+MPNIGAFIAWG+ITALFIPTGWLPN+ A LV PMI +LLPLLIG Sbjct: 8 RVKVQRFGSYLSGMIMPNIGAFIAWGIITALFIPTGWLPNETFAKLVGPMITYLLPLLIG 67 Query: 75 YTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKAG 134 YTGG+MI RGGV+GA ATMGVIVG+DIPMFLGAMI+GPL G+ ++ FD+ GK+K G Sbjct: 68 YTGGKMIYDVRGGVVGATATMGVIVGSDIPMFLGAMIMGPLGGYAIKQFDKLFHGKVKQG 127 Query: 135 FEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPAK 194 FEMLVNNFS A+KGIGP+V GL L+ GVE +VN LLPL NI IEPAK Sbjct: 128 FEMLVNNFSAGIIGGLLTLVAFKGIGPIVLGLNKTLAAGVETIVNAKLLPLANIFIEPAK 187 Query: 195 VLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAPG 254 VLFLNNAINHGILSP+ E++A+ GKSILF+LE+NPGPGLGILLAYWL GKG AK SAPG Sbjct: 188 VLFLNNAINHGILSPLGVEQAAKTGKSILFLLETNPGPGLGILLAYWLFGKGMAKQSAPG 247 Query: 255 AVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYLA 314 AVIIHFLGGIHEIYFPYILM P LILA I GGVSG FTF L AGL + PSPGSIFA LA Sbjct: 248 AVIIHFLGGIHEIYFPYILMRPVLILAAIAGGVSGVFTFTLFNAGLVAVPSPGSIFALLA 307 Query: 315 MTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSKNNEDDVDLEEASAKMREMKXXXXXXX 374 MTP+G Y + AGVIVA VSF KTS +++ DLE+A+ +M+++K Sbjct: 308 MTPRGHYLGVLAGVIVAAAVSFLVASFFLKTS---KQEEGDLEQATEQMQQLK------- 357 Query: 375 XXXXXXXXNVITPNVTANVRNKADVNKIVFACDAGMGSSAMGASVLRKKLQNAGVNITVV 434 +V + TA R V KIVFACDAGMGSSAMGAS++R K+Q AG++I V Sbjct: 358 ----GKKSSVASALNTAAPR---QVKKIVFACDAGMGSSAMGASIMRNKVQKAGLDIEVT 410 Query: 435 NSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERLK 487 N+A++++PADAD+V+T + LTDRA PNA H+S++NFL SPKYDEL+E LK Sbjct: 411 NTAINQLPADADVVITHQNLTDRAKEKLPNAYHVSVENFLNSPKYDELIEMLK 463 >P50852.1 PTMCB_BACST PTS system mannitol-specific EIICB component Mannitol permease IIC component Mannitol-specific phosphotransferase enzyme IIB component EC=2.7.1.69 Length = 471 Score = 591 bits (1523), Expect = e-167, Method: Compositional matrix adjust. Identities = 299/478 (62%), Positives = 362/478 (75%), Gaps = 24/478 (5%) Query: 15 RVKVQQFGRMLSGMVMPNIGAFIAWGLITALFIPTGWLPNKDLAMLVDPMIKFLLPLLIG 74 RVK+Q+FG LSGM+MPNIGAFIAWG+ITALFIPTGWLPN+ A LV PMI +LLPLLIG Sbjct: 12 RVKIQRFGSYLSGMIMPNIGAFIAWGIITALFIPTGWLPNETFAKLVGPMITYLLPLLIG 71 Query: 75 YTGGQMINGKRGGVIGAIATMGVIVGADIPMFLGAMIVGPLAGWVLRLFDRAVEGKIKAG 134 YTGG+MI RGGV+GA ATMGVIVG+DIPMFLGAMI+GPL G++++ FD+ ++GK+K G Sbjct: 72 YTGGKMIYDVRGGVVGATATMGVIVGSDIPMFLGAMIMGPLGGYLIKKFDQQIQGKVKQG 131 Query: 135 FEMLVNNFSXXXXXXXXXXXAYKGIGPLVQGLTNILSNGVEFLVNHNLLPLVNIIIEPAK 194 FEMLVNNFS A+KG+GP+V ++ L+ GVE +V+ +LLPL NI IEP K Sbjct: 132 FEMLVNNFSAGIIGGLLTLAAFKGVGPVVSAISKTLAAGVEKIVDLHLLPLANIFIEPGK 191 Query: 195 VLFLNNAINHGILSPIAAEESARIGKSILFMLESNPGPGLGILLAYWLVGKGSAKSSAPG 254 VLFLNNAINHGILSP+ E++A+ GKSILF+LE NPGPGLGILLAYWL GKG AK SAPG Sbjct: 192 VLFLNNAINHGILSPLGIEQAAKTGKSILFLLEPNPGPGLGILLAYWLFGKGMAKQSAPG 251 Query: 255 AVIIHFLGGIHEIYFPYILMNPRLILAVIGGGVSGTFTFQLLGAGLTSSPSPGSIFAYLA 314 A+IIHFLGGIHEIYFPY+LM P LILA I GGVSG TF + AGL + PSPGSIFA LA Sbjct: 252 AIIIHFLGGIHEIYFPYVLMRPILILAAIAGGVSGVLTFTIFDAGLVAVPSPGSIFALLA 311 Query: 315 MTPKGGYGPMFAGVIVATVVSFXXXXXXXKTSSKNNEDDVDLEEASAKMREMKXXXXXXX 374 MTPKG Y + AGV+VAT VSF K S+KNNE+D+ +A+ KM+++K Sbjct: 312 MTPKGNYLGVLAGVLVATAVSFFVASIFLK-SAKNNEEDIT--KATEKMQQLKGK----- 363 Query: 375 XXXXXXXXNVITPNVTANVRNK-----ADVNKIVFACDAGMGSSAMGASVLRKKLQNAGV 429 +V A ++N+ A V KIVFACDAGMGSSAMGAS+LR K+Q AG+ Sbjct: 364 -----------KSDVVAVLKNEEKVIPAKVKKIVFACDAGMGSSAMGASILRNKMQKAGL 412 Query: 430 NITVVNSAVSEIPADADIVVTQKTLTDRAIASNPNAEHISIDNFLKSPKYDELVERLK 487 NI V N+A++++P DADIV+T + LTDRA P A HIS++NFL SPKYDEL+E LK Sbjct: 413 NIEVTNTAINQLPEDADIVITHQNLTDRAKEKLPKAFHISVENFLNSPKYDELIEMLK 470 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.139 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,631,897,492 Number of extensions: 112006324 Number of successful extensions: 308118 Number of sequences better than 1.0e-05: 430 Number of HSP's gapped: 307253 Number of HSP's successfully gapped: 471 Length of query: 487 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 347 Effective length of database: 1,741,754,103 Effective search space: 604388673741 Effective search space used: 604388673741 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)