BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0029.p (144 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8D2.1 B3K8D2_9BACI Phosphoenolpyruvate-dependent sugar phosph... 211 2e-53 B1DC77.1 B1DC77_9BACL Phosphoenolpyruvate-dependent sugar phosph... 179 8e-44 C0Z8I7.1 C0Z8I7_BREBN PTS system IIA component EC=2.7.1.69 159 7e-38 C0H3V2.1 PTMA_BACSU Mannitol-specific phosphotransferase enzyme ... 140 4e-32 Q65NA2.1 Q65NA2_BACLD Putative uncharacterized protein 137 3e-31 >B3K8D2.1 B3K8D2_9BACI Phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2 Length = 146 Score = 211 bits (536), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 99/142 (69%), Positives = 123/142 (86%) Query: 2 SILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAIP 61 +ILS++KV++N A K EAI MAG LLV AGHV+ +YV KMLEREE+ STYMGGGLAIP Sbjct: 3 NILSKDKVVLNAKADSKSEAIRMAGNLLVQAGHVSADYVDKMLEREEIASTYMGGGLAIP 62 Query: 62 HGTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTEDD 121 HGTKEA+ + STGLS++RFP+GVDFGGDEPAF+VIGIAAAG HME+LTNVAMIFT+D+ Sbjct: 63 HGTKEAKGMVISTGLSIVRFPEGVDFGGDEPAFIVIGIAAAGGDHMEILTNVAMIFTDDE 122 Query: 122 AIERVMNAPTPADVIAIFEGGL 143 +IE+VMNA + +++AIFEGG+ Sbjct: 123 SIEKVMNASSEEEILAIFEGGM 144 >B1DC77.1 B1DC77_9BACL Phosphoenolpyruvate-dependent sugar phosphotransferase system EIIA 2 Length = 144 Score = 179 bits (453), Expect = 8e-44, Method: Compositional matrix adjust. Identities = 84/144 (58%), Positives = 112/144 (77%) Query: 1 MSILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAI 60 MSILS +KV +N A KDK+EA+ +AG+LLV+AGH YV M+ERE+ +STYMGGGLAI Sbjct: 1 MSILSTDKVKLNAAVKDKFEAVRLAGELLVNAGHADPSYVESMVEREQSLSTYMGGGLAI 60 Query: 61 PHGTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTED 120 PHGT E++ IKSTGLS+++ P GVDFG A +VIGIAA GD H+++LTNVAM+ ++D Sbjct: 61 PHGTNESKGSIKSTGLSIVQIPAGVDFGEGNTAHLVIGIAAVGDDHLDILTNVAMVCSDD 120 Query: 121 DAIERVMNAPTPADVIAIFEGGLE 144 D +ER++ A T ++I IFE G+E Sbjct: 121 DNLERILKASTAEEMIEIFESGME 144 >C0Z8I7.1 C0Z8I7_BREBN PTS system IIA component EC=2.7.1.69 Length = 144 Score = 159 bits (403), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 68/138 (49%), Positives = 109/138 (78%) Query: 2 SILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAIP 61 +IL+ K+++N +K EAI +AG+LLV AGHVTE Y+ KM ERE++ +TY+G G+AIP Sbjct: 3 NILTAEKIMLNAKVANKEEAIRLAGQLLVRAGHVTEAYLKKMQEREQLATTYIGSGVAIP 62 Query: 62 HGTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTEDD 121 HGT E++ I+STG+S+I+ P+GVDFG A++++GIAA GD H+EVL+N+A++ +E++ Sbjct: 63 HGTNESKAEIQSTGISIIQVPEGVDFGEGNTAYLLVGIAAVGDEHLEVLSNIAILCSEEE 122 Query: 122 AIERVMNAPTPADVIAIF 139 ++R++NA + ++IA+F Sbjct: 123 NVKRIVNAASAEEIIAMF 140 >C0H3V2.1 PTMA_BACSU Mannitol-specific phosphotransferase enzyme IIA component EC=2.7.1.- Length = 143 Score = 140 bits (353), Expect = 4e-32, Method: Compositional matrix adjust. Identities = 62/139 (44%), Positives = 100/139 (71%) Query: 1 MSILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAI 60 M +L++ + +N K EAI +AG+ L+D G+VTE+Y+ KM EREE ST+MG +AI Sbjct: 1 MQVLAKENIKLNQTVSSKEEAIKLAGQTLIDNGYVTEDYISKMFEREETSSTFMGNFIAI 60 Query: 61 PHGTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTED 120 PHGT+EA+ + +G+S+I+ P+GV++G A VV GIA + H+++L+N+A+I +E+ Sbjct: 61 PHGTEEAKSEVLHSGISIIQIPEGVEYGEGNTAKVVFGIAGKNNEHLDILSNIAIICSEE 120 Query: 121 DAIERVMNAPTPADVIAIF 139 + IER+++A + D+IAIF Sbjct: 121 ENIERLISAKSEEDLIAIF 139 >Q65NA2.1 Q65NA2_BACLD Putative uncharacterized protein Length = 146 Score = 137 bits (346), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 62/140 (44%), Positives = 101/140 (72%) Query: 3 ILSENKVIMNGAAKDKYEAITMAGKLLVDAGHVTEEYVPKMLEREEVVSTYMGGGLAIPH 62 +L++ + +N +A + EAI +AGK LVDAG+VT +Y+ KMLERE + STYMG +AIPH Sbjct: 4 VLAKENIHLNQSAATQEEAIRLAGKALVDAGYVTADYIEKMLEREGLTSTYMGNAIAIPH 63 Query: 63 GTKEARPFIKSTGLSVIRFPDGVDFGGDEPAFVVIGIAAAGDGHMEVLTNVAMIFTEDDA 122 GT++++ + +G+SVI+ P+GV++G A V+ GIA D H+E+L+ +A++ +E D Sbjct: 64 GTEDSKQDVLHSGISVIQLPEGVEYGDGNIAKVIFGIAGKNDEHLEILSKIAIVCSEADN 123 Query: 123 IERVMNAPTPADVIAIFEGG 142 +ER++NA +++A+FE G Sbjct: 124 VERIINAKNEDELMAVFEEG 143 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 883,318,348 Number of extensions: 36939174 Number of successful extensions: 86881 Number of sequences better than 1.0e-05: 1030 Number of HSP's gapped: 87418 Number of HSP's successfully gapped: 1030 Length of query: 144 Length of database: 3,060,696,203 Length adjustment: 107 Effective length of query: 37 Effective length of database: 2,052,647,598 Effective search space: 75947961126 Effective search space used: 75947961126 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)