BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0028.p (380 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K8D1.1 B3K8D1_9BACI Mannitol dehydrogenase domain 503 e-140 B1DC78.1 B1DC78_9BACL Mannitol dehydrogenase domain 446 e-123 Q45421.1 MTLD_BACST Mannitol-1-phosphate 5-dehydrogenase EC=1.1.... 350 2e-94 B7GJR4.1 MTLD_ANOFW Mannitol-1-phosphate 5-dehydrogenase EC=1.1.... 344 9e-93 C3J4B5.1 C3J4B5_9BACI Mannitol dehydrogenase domain protein 340 1e-91 >B3K8D1.1 B3K8D1_9BACI Mannitol dehydrogenase domain Length = 389 Score = 503 bits (1294), Expect = e-140, Method: Compositional matrix adjust. Identities = 248/370 (67%), Positives = 288/370 (77%), Gaps = 1/370 (0%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIGLLLSQAGYEVCFVDVNE VS+L+ R EYPVTLASEGQET +VK +TAL+S + Sbjct: 13 RGFIGLLLSQAGYEVCFVDVNEELVSELKARGEYPVTLASEGQETTVVKGITALNSASEP 72 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAVSNA-PLHVIACENAIGGSAQL 130 E A ++AEADLVTTAVGV ILK IAG +A+G++ R+ +A PLH+IACENAIGGS+QL Sbjct: 73 EAAARAVAEADLVTTAVGVNILKFIAGTIAEGLKLRLQTDSASPLHIIACENAIGGSSQL 132 Query: 131 KELVYAKLDGESRSKADVSVAFPNAAVDRIVPLQQHEDILKVVVEPFYEWVIDSSQMIPG 190 KE VY KLD E++ KAD VAFP+AAVDRIVPLQQHED LKVVVEPFYEW +D SQM Sbjct: 133 KEHVYGKLDDETKRKADRLVAFPDAAVDRIVPLQQHEDRLKVVVEPFYEWAVDRSQMFED 192 Query: 191 YVPVAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVREVL 250 YV V G+HYVD LEPYIERKLFTVNTGHCSAAY+G+L+GYETIQ AM DEA+ VR VL Sbjct: 193 YVSVEGIHYVDRLEPYIERKLFTVNTGHCSAAYVGFLKGYETIQDAMKDEAVAGHVRAVL 252 Query: 251 EETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSPAL 310 EETGA LI KH FD ++H YID ILERFRNPALTDEVSRVGRSP+RKLSP DRLVSPA+ Sbjct: 253 EETGAALIHKHAFDPSEHQAYIDTILERFRNPALTDEVSRVGRSPIRKLSPGDRLVSPAM 312 Query: 311 QAYDRGLGYTALTRSMAGALLFDVKDDPXXXXXXXXXXXXXXXXXVTKYTGIAADHTVHQ 370 A++ GL Y +L +SMA ALLFDV +DP ++YTGIA +H + Sbjct: 313 LAFESGLAYKSLAKSMAAALLFDVPEDPESVELQASVRDIGVSDTASQYTGIAGEHPIMG 372 Query: 371 SIMSEYAKLK 380 +M +Y LK Sbjct: 373 EVMEQYEVLK 382 >B1DC78.1 B1DC78_9BACL Mannitol dehydrogenase domain Length = 383 Score = 446 bits (1148), Expect = e-123, Method: Compositional matrix adjust. Identities = 219/365 (60%), Positives = 270/365 (73%), Gaps = 1/365 (0%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIGLLLSQ+GYEV F DVN+A V++LQ+R Y VTLA+E ++ + V VTA+ T Sbjct: 13 RGFIGLLLSQSGYEVIFSDVNQALVTELQKRGAYSVTLANEAKDQLQVTGVTAIDG-TKI 71 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAVSNAPLHVIACENAIGGSAQLK 131 ++V A++AEADLVTTAVGV IL+HIA +A GI KR+ PLH+IACENAIGGS QLK Sbjct: 72 DDVTAAVAEADLVTTAVGVNILRHIAAGIARGIEKRIESGGGPLHIIACENAIGGSTQLK 131 Query: 132 ELVYAKLDGESRSKADVSVAFPNAAVDRIVPLQQHEDILKVVVEPFYEWVIDSSQMIPGY 191 E V+ LD E+++KA +AFP+AAVDRIVP+Q +ED L+V VEPFYEWV+D + M GY Sbjct: 132 EHVFGHLDEETKAKASKLIAFPDAAVDRIVPIQHNEDPLEVTVEPFYEWVVDETAMFEGY 191 Query: 192 VPVAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVREVLE 251 PVAGVHYV NL PYIERKLFTVNTGHC+AAYLG LRGYETIQQAMADEA+ +V+ L Sbjct: 192 KPVAGVHYVSNLMPYIERKLFTVNTGHCAAAYLGSLRGYETIQQAMADEAIVGEVKATLR 251 Query: 252 ETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSPALQ 311 ETGAVL+ ++GFD+A+H +YI+KI+ RFRN LTDEV+RVGRSP+RKLSPNDRLV PALQ Sbjct: 252 ETGAVLVAQYGFDQAEHDRYIEKIIGRFRNDQLTDEVTRVGRSPIRKLSPNDRLVKPALQ 311 Query: 312 AYDRGLGYTALTRSMAGALLFDVKDDPXXXXXXXXXXXXXXXXXVTKYTGIAADHTVHQS 371 A+++G+ L + MA AL F DDP V+ YT I H V+ Sbjct: 312 AHEQGIKPVQLVKVMAAALNFVNADDPEAVELQQSIKDKGIGQTVSHYTSIPEGHPVYGL 371 Query: 372 IMSEY 376 I+ Y Sbjct: 372 IIESY 376 >Q45421.1 MTLD_BACST Mannitol-1-phosphate 5-dehydrogenase EC=1.1.1.17 Length = 382 Score = 350 bits (897), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 171/370 (46%), Positives = 247/370 (66%), Gaps = 1/370 (0%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIG LLSQ+GYEV FVD+N+ V L+E++EY V +A E ++ ++++NV+A++S T Sbjct: 13 RGFIGSLLSQSGYEVVFVDINDELVRLLKEKQEYRVIIADENRQELLIRNVSAVNSQTER 72 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAVSNAPLHVIACENAIGGSAQLK 131 E+V I +A L+TTAVG IL IA ++A+G++KR+ ++ PLH+IACEN IGGS LK Sbjct: 73 EKVIDYITKAHLITTAVGPHILPAIATILAEGLQKRITINKTPLHIIACENMIGGSDVLK 132 Query: 132 ELVYAKLDGESRSKADVSVAFPNAAVDRIVPLQQHEDILKVVVEPFYEWVIDSSQMIPGY 191 V+ K+ + + F N AVDRIVP Q+H+D L VVVEPF+EWVI+ +I Sbjct: 133 SHVFEKISEADKPLFEKYYGFLNCAVDRIVPNQKHDDPLSVVVEPFFEWVIEKRNIIGAI 192 Query: 192 VPVAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVREVLE 251 P+ G H+VD+L+PYIERKLFTVNTGH A+YLGY + +TIQ+AM D+ + + V + L Sbjct: 193 PPIQGAHFVDDLKPYIERKLFTVNTGHAIASYLGYYKKLQTIQEAMCDQEIRSDVEKALH 252 Query: 252 ETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSPALQ 311 E+GAVL++K+G+++ +H YI KI++RF NP+++DEV RV RSP+RKL NDRL+ PA Q Sbjct: 253 ESGAVLVKKYGWNENEHQSYIQKIIQRFINPSISDEVVRVARSPIRKLGANDRLIGPATQ 312 Query: 312 AYDR-GLGYTALTRSMAGALLFDVKDDPXXXXXXXXXXXXXXXXXVTKYTGIAADHTVHQ 370 YD G L + +A LLFD ++D + +Y+ + DH + Sbjct: 313 YYDLFGQVPHGLVKGIAALLLFDYENDEEAVALQKTIQETGVEGALYQYSQLEKDHPLVI 372 Query: 371 SIMSEYAKLK 380 +I ++ LK Sbjct: 373 AIKDQWQHLK 382 >B7GJR4.1 MTLD_ANOFW Mannitol-1-phosphate 5-dehydrogenase EC=1.1.1.17 Length = 379 Score = 344 bits (882), Expect = 9e-93, Method: Compositional matrix adjust. Identities = 171/358 (47%), Positives = 237/358 (66%), Gaps = 3/358 (0%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIG LLSQ+ Y V FVDVNE V LQ++ +Y V +A E +T +V+NV+AL S Sbjct: 13 RGFIGSLLSQSRYNVVFVDVNEKIVQALQQKGQYEVIIAGETTQTQVVRNVSALHSQHQQ 72 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAVSNAPLHVIACENAIGGSAQLK 131 E+ IA ADLVTTAVG IL I+ +A+G++KR+ ++ P+H+IACEN IGGS LK Sbjct: 73 NEIIDQIARADLVTTAVGPNILPLISQTIAEGLKKRL--TDRPVHIIACENMIGGSEHLK 130 Query: 132 ELVYAKLDGESRSKADVSVAFPNAAVDRIVPLQQHEDILKVVVEPFYEWVIDSSQMIPGY 191 V+ L + ++ + F N AVDRIVP Q HED L VVVEPF+EWVI++ ++I Sbjct: 131 TYVWEHLSEQEQTSLEDKCGFLNCAVDRIVPNQTHEDPLTVVVEPFFEWVIETKEVIGNI 190 Query: 192 VPVAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVREVLE 251 + G H+VD+L+PYIERKLFTVNTGH AYLGY + YETI+QAM D+ + A V L Sbjct: 191 PHIIGAHFVDDLQPYIERKLFTVNTGHALVAYLGYRKKYETIRQAMEDQDILADVTNALR 250 Query: 252 ETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSPALQ 311 E+G VL+ ++G+++A+H YI+KI++RF NP++TDEV+RV RSP+RKL PNDRL+ PA++ Sbjct: 251 ESGRVLVHQYGWNEAEHQAYIEKIVQRFINPSMTDEVTRVARSPIRKLGPNDRLIRPAMK 310 Query: 312 AYD-RGLGYTALTRSMAGALLFDVKDDPXXXXXXXXXXXXXXXXXVTKYTGIAADHTV 368 Y+ G +L + +A LLFD + D + KY + ++H + Sbjct: 311 YYECFGEVPASLAKGIAALLLFDYEGDAEAVQLQQTIKESGVEGALEKYAKLPSNHPI 368 >C3J4B5.1 C3J4B5_9BACI Mannitol dehydrogenase domain protein Length = 386 Score = 340 bits (873), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 180/370 (48%), Positives = 235/370 (63%), Gaps = 1/370 (0%) Query: 12 RGFIGLLLSQAGYEVCFVDVNEAFVSQLQERKEYPVTLASEGQETVIVKNVTALSSVTHA 71 RGFIG LL+ +GY+V FVDVNE V L+ER EY V +A E QE V+ V+AL+S T Sbjct: 13 RGFIGSLLAASGYDVVFVDVNEQIVRLLKERGEYRVIVAGEQQEEQWVRGVSALNSQTER 72 Query: 72 EEVAASIAEADLVTTAVGVTILKHIAGVVADGIRKRVAVSNAPLHVIACENAIGGSAQLK 131 E V +IA+ADLVTTAVG IL IA VVA G+++R A+ PLHVIACEN I G+ LK Sbjct: 73 EAVIEAIADADLVTTAVGPPILPAIAPVVAAGLQRRFAIQQKPLHVIACENMIAGTEALK 132 Query: 132 ELVYAKLDGESRSKADVSVAFPNAAVDRIVPLQQHEDILKVVVEPFYEWVIDSSQMIPGY 191 V+ L + + A+ SV F N AVDRIVP Q ++D L V VEPF+EW I++ +I Sbjct: 133 THVFEHLSADEQRLANQSVGFLNCAVDRIVPNQTNDDPLAVTVEPFFEWAIEARNVIGAV 192 Query: 192 VPVAGVHYVDNLEPYIERKLFTVNTGHCSAAYLGYLRGYETIQQAMADEALTAQVREVLE 251 P+ G H+V +L PYIERKLFTVNTGH AAYLGYLR YET+Q+AM DE + V + L Sbjct: 193 PPIQGAHFVADLGPYIERKLFTVNTGHALAAYLGYLRNYETVQEAMNDEDIRWNVEQALS 252 Query: 252 ETGAVLIQKHGFDKAQHSKYIDKILERFRNPALTDEVSRVGRSPLRKLSPNDRLVSPALQ 311 E+GAVL++KHG+D +H YID + RF NP+L+D++ RV RSP+RKL PNDRLV+PA Q Sbjct: 253 ESGAVLVKKHGWDANEHRAYIDTTIGRFMNPSLSDDIIRVARSPIRKLGPNDRLVAPATQ 312 Query: 312 AYDRGLGYT-ALTRSMAGALLFDVKDDPXXXXXXXXXXXXXXXXXVTKYTGIAADHTVHQ 370 Y L + +A L FD D + +Y G+ H + Sbjct: 313 YYALFERVPFGLVKGIAALLRFDDASDAEAAALQQTLKQRGVEEALWQYAGLDRGHPLIA 372 Query: 371 SIMSEYAKLK 380 ++ EY +++ Sbjct: 373 AVKEEYGRME 382 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,945,675,285 Number of extensions: 74390924 Number of successful extensions: 215480 Number of sequences better than 1.0e-05: 1224 Number of HSP's gapped: 212540 Number of HSP's successfully gapped: 1224 Length of query: 380 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 243 Effective length of database: 1,770,017,148 Effective search space: 430114166964 Effective search space used: 430114166964 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)