BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0021.p (359 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFX1.1 B3KFX1_9BACI Glutamyl aminopeptidase EC=3.4.11.7 587 e-166 Q9K7V2.1 Q9K7V2_BACHD Endo-1,4-beta-glucanase 493 e-137 A7GTW6.1 A7GTW6_BACCN Glutamyl aminopeptidase EC=3.4.11.7 492 e-137 C2WD90.1 C2WD90_BACCE Deblocking aminopeptidase 492 e-137 C3BR68.1 C3BR68_9BACI Deblocking aminopeptidase 491 e-137 >B3KFX1.1 B3KFX1_9BACI Glutamyl aminopeptidase EC=3.4.11.7 Length = 357 Score = 587 bits (1513), Expect = e-166, Method: Compositional matrix adjust. Identities = 276/359 (76%), Positives = 317/359 (88%), Gaps = 2/359 (0%) Query: 1 MNPETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENG 60 MN TL+LF+TLTEFPAAPGFERELR++VKDAL+PYTDEFVQDRLGSL VLRG + G Sbjct: 1 MNESTLELFRTLTEFPAAPGFERELRSWVKDALSPYTDEFVQDRLGSLF--AVLRGNDEG 58 Query: 61 PKIMVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTP 120 PK+MVAGH DEVGFMVTGIT+ GM+RFQPLGGW + AV +QRLQIIT G + GVVGS P Sbjct: 59 PKVMVAGHMDEVGFMVTGITEAGMVRFQPLGGWWSQAVLAQRLQIITDNGPVVGVVGSIP 118 Query: 121 IHLQSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKA 180 HL + +R +P DI MYIDIGAD++ EA+SFG+ PG Q++P+C FTPLANPKKIMAKA Sbjct: 119 THLLDEAQRSKPVDINTMYIDIGADNKAEAESFGIRPGLQVVPICEFTPLANPKKIMAKA 178 Query: 181 WDNRYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASA 240 WDNRYGVGLAIELVQALHG+KLPNTV+AGATVQEE GLRGART+ANL+QPDI+F LDASA Sbjct: 179 WDNRYGVGLAIELVQALHGEKLPNTVYAGATVQEELGLRGARTSANLIQPDIFFGLDASA 238 Query: 241 AADMTGDRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQYFVSQGGTDAG 300 A DM GD+ FGQLGQGALLRI+DPTM+THRG++EYVQD A+T+KIKYQYFVS GGTDAG Sbjct: 239 ANDMMGDKSQFGQLGQGALLRIFDPTMLTHRGMVEYVQDTAETNKIKYQYFVSPGGTDAG 298 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTILENS 359 QVH+SGIGVPS +IGICSRYIHT+SSIIH+DDY AAKELLIKLV+GLDRTTL TIL+ + Sbjct: 299 QVHLSGIGVPSTIIGICSRYIHTSSSIIHTDDYEAAKELLIKLVKGLDRTTLQTILDRA 357 >Q9K7V2.1 Q9K7V2_BACHD Endo-1,4-beta-glucanase Length = 357 Score = 493 bits (1268), Expect = e-137, Method: Compositional matrix adjust. Identities = 231/358 (64%), Positives = 286/358 (79%), Gaps = 2/358 (0%) Query: 1 MNPETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENG 60 MN ET LFKTLTE APGFE +R +V+ L YT+E VQDRLGS+ GV RG E G Sbjct: 1 MNQETQSLFKTLTELQGAPGFEHHIRRFVRGELEKYTNEIVQDRLGSIF--GVKRGNEQG 58 Query: 61 PKIMVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTP 120 PK+MVAGH DEVGFMVT I +KG+IRFQ LGGW + + +QR+QI+T +G + GV+GSTP Sbjct: 59 PKVMVAGHMDEVGFMVTSINEKGLIRFQTLGGWWSQVLLAQRVQIMTDEGPVIGVIGSTP 118 Query: 121 IHLQSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKA 180 HL + +RK+P D++ MYIDIGAD +E+AQ G+ PGQQI+P+CPFTPLAN KKIMAKA Sbjct: 119 PHLLEEAQRKKPMDVKNMYIDIGADDKEDAQKIGIKPGQQIVPICPFTPLANEKKIMAKA 178 Query: 181 WDNRYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASA 240 WDNRYGVGLAIEL++ L G+ PN +++GATVQEE GLRGA T+A +++PDI++ALDAS Sbjct: 179 WDNRYGVGLAIELLKELQGETTPNILYSGATVQEEVGLRGAATSAQMIEPDIFYALDASP 238 Query: 241 AADMTGDRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQYFVSQGGTDAG 300 A D T + AFGQLG+GAL+RIYD TM+THRG+ ++V D A+T I YQ+F+SQGGTDAG Sbjct: 239 ANDATAGKDAFGQLGKGALVRIYDRTMVTHRGIRDFVLDTAETENIPYQFFISQGGTDAG 298 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTILEN 358 +VH+SG GVPSAVIGICSRYIHTA+SIIH DDYAAAK LL+KLV+ D+ + TIL N Sbjct: 299 RVHLSGNGVPSAVIGICSRYIHTAASIIHVDDYAAAKALLVKLVKTTDKAAVETILAN 356 >A7GTW6.1 A7GTW6_BACCN Glutamyl aminopeptidase EC=3.4.11.7 Length = 357 Score = 492 bits (1266), Expect = e-137, Method: Compositional matrix adjust. Identities = 228/355 (64%), Positives = 287/355 (80%), Gaps = 2/355 (0%) Query: 1 MNPETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENG 60 MN ETL+LF+TLTE A GFE ++R ++K L+ Y DE VQD LGS+ G+ +G+E+G Sbjct: 1 MNKETLELFRTLTELQGASGFEHDVRRFMKQELSKYADEIVQDGLGSIF--GLKKGDESG 58 Query: 61 PKIMVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTP 120 P+++VAGH DEVGFMVT IT+ GMIRFQ LGGW + + +QR+QI+T G + GV+GS P Sbjct: 59 PRVLVAGHMDEVGFMVTQITENGMIRFQTLGGWWSQVLLAQRVQIMTKNGPIVGVIGSIP 118 Query: 121 IHLQSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKA 180 HL SD +R +P DI+ M IDIGADS EEA GV PGQQI+P+CPFTP+AN KKIMAKA Sbjct: 119 PHLLSDAQRAKPMDIKNMLIDIGADSYEEALEIGVKPGQQIVPICPFTPMANEKKIMAKA 178 Query: 181 WDNRYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASA 240 WDNRYG GLAIEL++ L + LPN +++GATVQEE GLRGA+TAAN++QPDI++ALDAS Sbjct: 179 WDNRYGCGLAIELLKELKDETLPNILYSGATVQEEVGLRGAQTAANMIQPDIFYALDASP 238 Query: 241 AADMTGDRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQYFVSQGGTDAG 300 A D +GD++ FGQLG+GALLRIYD TM+THRG+ E++ D A+TH I YQYF+SQGGTDAG Sbjct: 239 ANDASGDKEQFGQLGKGALLRIYDRTMVTHRGMREFILDTAETHNIPYQYFISQGGTDAG 298 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTI 355 +VH S G+PSAVIG+C+RYIHT +SI+H DDYAAAKELL+KLV+ D+TTL TI Sbjct: 299 RVHTSNSGIPSAVIGVCARYIHTHASILHVDDYAAAKELLMKLVKATDKTTLETI 353 >C2WD90.1 C2WD90_BACCE Deblocking aminopeptidase Length = 363 Score = 492 bits (1266), Expect = e-137, Method: Compositional matrix adjust. Identities = 229/359 (63%), Positives = 286/359 (79%), Gaps = 2/359 (0%) Query: 1 MNPETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENG 60 +N ETL+LF+TLTE GFE ++R ++K L+ Y DE VQD LGS+ G+ +G+ENG Sbjct: 7 VNKETLELFRTLTELQGTSGFEHDVRRFMKQELSKYADEIVQDGLGSVF--GLKKGDENG 64 Query: 61 PKIMVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTP 120 P+++VAGH DEVGFMVT IT GMIRFQ LGGW + + +QR+QI+T G + GVVGS P Sbjct: 65 PRVLVAGHMDEVGFMVTQITKNGMIRFQTLGGWWSQVLLAQRVQIMTKNGPIVGVVGSIP 124 Query: 121 IHLQSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKA 180 HL SD +R +P DI+ M IDIGADS E+A GV PGQQI+P+CPFTP+AN KKIMAKA Sbjct: 125 PHLLSDAQRAKPMDIKNMLIDIGADSYEDALEIGVKPGQQIVPICPFTPMANEKKIMAKA 184 Query: 181 WDNRYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASA 240 WDNRYG GLAIEL++ L +KLPNT+++GATVQEE GLRGA+TAAN++QPDI++ALDAS Sbjct: 185 WDNRYGCGLAIELLKELKDEKLPNTLYSGATVQEEVGLRGAQTAANMIQPDIFYALDASP 244 Query: 241 AADMTGDRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQYFVSQGGTDAG 300 A D +GD+ FGQLG+GALLRIYD TM+THRG+ E++ D A+TH I YQYF+SQGGTDAG Sbjct: 245 ANDASGDKTQFGQLGKGALLRIYDRTMVTHRGMREFILDTAETHNIPYQYFISQGGTDAG 304 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTILENS 359 +VH S G+PSAVIG+C+RYIHT +SI+H DDYAAAKEL+ KLV D+TTL TI N+ Sbjct: 305 RVHTSNSGIPSAVIGVCARYIHTHASILHVDDYAAAKELITKLVRATDKTTLETIKNNA 363 >C3BR68.1 C3BR68_9BACI Deblocking aminopeptidase Length = 363 Score = 491 bits (1264), Expect = e-137, Method: Compositional matrix adjust. Identities = 228/359 (63%), Positives = 287/359 (79%), Gaps = 2/359 (0%) Query: 1 MNPETLDLFKTLTEFPAAPGFERELRAYVKDALAPYTDEFVQDRLGSLXXXGVLRGEENG 60 +N ETL+LF+TLTE GFE ++R ++K+ L+ Y DE VQD LGS+ G+ +G+ENG Sbjct: 7 VNKETLELFRTLTELQGTSGFEHDVRRFMKEELSKYADEIVQDGLGSVF--GLKKGDENG 64 Query: 61 PKIMVAGHFDEVGFMVTGITDKGMIRFQPLGGWLASAVASQRLQIITPKGTLTGVVGSTP 120 P+++VAGH DEVGFMVT IT GMIRFQ LGGW + + +QR+QI+T G + GVVGS P Sbjct: 65 PRVLVAGHMDEVGFMVTQITKNGMIRFQTLGGWWSQVLLAQRVQIMTKNGPVVGVVGSIP 124 Query: 121 IHLQSDEERKQPGDIRKMYIDIGADSREEAQSFGVAPGQQILPVCPFTPLANPKKIMAKA 180 HL SD +R +P DI+ M IDIGADS E+A GV PGQQI+P+CPFTP+AN KKIMAKA Sbjct: 125 PHLLSDAQRAKPMDIKNMLIDIGADSYEQALEIGVKPGQQIVPICPFTPMANEKKIMAKA 184 Query: 181 WDNRYGVGLAIELVQALHGKKLPNTVFAGATVQEEAGLRGARTAANLLQPDIYFALDASA 240 WDNRYG GLAIEL++ L + LPNT+++GATVQEE GLRGA+TAAN++QPDI++ALDAS Sbjct: 185 WDNRYGCGLAIELLKELKDETLPNTLYSGATVQEEVGLRGAQTAANMIQPDIFYALDASP 244 Query: 241 AADMTGDRQAFGQLGQGALLRIYDPTMITHRGLIEYVQDMADTHKIKYQYFVSQGGTDAG 300 A D +GD+ FGQLG+GALLRIYD TM+THRG+ E++ D A+TH I YQYF+SQGGTDAG Sbjct: 245 ANDASGDKTQFGQLGKGALLRIYDRTMVTHRGMREFILDTAETHNIPYQYFISQGGTDAG 304 Query: 301 QVHVSGIGVPSAVIGICSRYIHTASSIIHSDDYAAAKELLIKLVEGLDRTTLHTILENS 359 +VH S G+PSAVIG+C+RYIHT +SI+H DDYAAAKEL+ KLV+ D+TTL TI N+ Sbjct: 305 RVHTSNSGIPSAVIGVCARYIHTHASILHVDDYAAAKELITKLVKATDKTTLETIKNNA 363 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,168,006,058 Number of extensions: 91565206 Number of successful extensions: 208506 Number of sequences better than 1.0e-05: 1082 Number of HSP's gapped: 205890 Number of HSP's successfully gapped: 1083 Length of query: 359 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 222 Effective length of database: 1,770,017,148 Effective search space: 392943806856 Effective search space used: 392943806856 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)