BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0018.p (344 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFX3.1 B3KFX3_9BACI Stage V sporulation protein AD 518 e-145 B1DBJ2.1 B1DBJ2_9BACL Stage V sporulation protein AD 483 e-134 A6TVT6.1 A6TVT6_ALKMQ Stage V sporulation AD family protein 443 e-122 B1DBI9.1 B1DBI9_9BACL Stage V sporulation protein AD 440 e-122 B0TIA6.1 B0TIA6_HELMI Stage v sporulation protein ad 437 e-121 >B3KFX3.1 B3KFX3_9BACI Stage V sporulation protein AD Length = 337 Score = 518 bits (1333), Expect = e-145, Method: Compositional matrix adjust. Identities = 242/341 (70%), Positives = 288/341 (84%), Gaps = 4/341 (1%) Query: 1 MKQLGSQTWQYTTRPVILGSSAVVGPEEGEGPLTSDFDFIYDSLKMGEKTWEKAERTLFE 60 MK +G QTWQ+ +PVI+G+ +VGPEEGEGPL +DFDF+YD++++ EKTWEKAER L E Sbjct: 1 MKVIG-QTWQFENKPVIIGTGTIVGPEEGEGPLANDFDFVYDNIEINEKTWEKAERKLLE 59 Query: 61 KASKLALINASLEQEQLEFFVGGDLMNQIISSSFAARQLGVPYLGVFGACSTSMESLAIA 120 AS+ A+ A ++++QL+FFVGGDLMNQIISSSFAARQ+GVPY+GVFGACSTSMESLA+A Sbjct: 60 DASRKAVEKAGIKEDQLQFFVGGDLMNQIISSSFAARQMGVPYIGVFGACSTSMESLAVA 119 Query: 121 SMIVDSGGGKYALAGTSSHNCTVEKQFRYPTEYGSQKPPTAQYTVTGSGCAVIGRDDGTS 180 SM+VDSGG +Y +AGT+SHNCTVEKQFRYPTEYGSQKPP AQYT+TG+GC+V+ Sbjct: 120 SMLVDSGGAQYVMAGTASHNCTVEKQFRYPTEYGSQKPPYAQYTITGAGCSVVSH---KG 176 Query: 181 AGPHVVMATMGRIMDLGLKDPFNMGXXXXXXXXDTITAHFRDTALSPGHYDLIVTGDLAS 240 GP V AT+G+++DLG+KDPFNMG DTIT HF+DT YDLIVTGDLAS Sbjct: 177 DGPAVTYATIGKVVDLGIKDPFNMGAAMAPAAADTITKHFKDTGRQAKDYDLIVTGDLAS 236 Query: 241 VGLPIAKDLLAKEGIPMEQTDFKDCGLMIYDLDKQKYVIAGGSGCGCSATVTYGHILKRM 300 VGLPIAKDLL + + M+ T F DCGLMIYD++KQKYV AGGSGCGCSA VTYGHILKRM Sbjct: 237 VGLPIAKDLLQQNDVIMDGTVFADCGLMIYDMEKQKYVNAGGSGCGCSAVVTYGHILKRM 296 Query: 301 KKGELKRVLIVATGALLSPLSYQQGETIPCVAHAVALEMGG 341 +KGELK+VL+VATGALLSPLSYQQGE+IPC+AHAVALEMGG Sbjct: 297 RKGELKKVLVVATGALLSPLSYQQGESIPCIAHAVALEMGG 337 >B1DBJ2.1 B1DBJ2_9BACL Stage V sporulation protein AD Length = 337 Score = 483 bits (1242), Expect = e-134, Method: Compositional matrix adjust. Identities = 225/336 (66%), Positives = 277/336 (82%), Gaps = 4/336 (1%) Query: 5 GSQTWQYTTRPVILGSSAVVGPEEGEGPLTSDFDFIYDSLKMGEKTWEKAERTLFEKASK 64 G QTW++ RPVI+G++ VVGP+EG+GPL DFD ++ L + +K+WEKAER L E+AS+ Sbjct: 4 GKQTWKFENRPVIIGAATVVGPDEGDGPLAGDFDLVHPELDLQQKSWEKAERLLLEQASE 63 Query: 65 LALINASLEQEQLEFFVGGDLMNQIISSSFAARQLGVPYLGVFGACSTSMESLAIASMIV 124 AL NA + ++QL+F+VGGDLMNQIIS+SFAAR LGVP LGVFGACSTSMESLAIAS++V Sbjct: 64 FALNNAKIAKDQLQFYVGGDLMNQIISNSFAARTLGVPNLGVFGACSTSMESLAIASLMV 123 Query: 125 DSGGGKYALAGTSSHNCTVEKQFRYPTEYGSQKPPTAQYTVTGSGCAVIGRDDGTSAGPH 184 DSG G+Y LAGT SHNCT EKQFRYPTEYG+QKPPTAQYTVTG+G V+ + GP Sbjct: 124 DSGAGQYVLAGTCSHNCTAEKQFRYPTEYGAQKPPTAQYTVTGAGAVVVA---ASGEGPV 180 Query: 185 VVMATMGRIMDLGLKDPFNMGXXXXXXXXDTITAHFRDTALSPGHYDLIVTGDLASVGLP 244 V AT+G+++DLG+KDPFNMG DTI AHF DT +P YDLIVTGDLASVG P Sbjct: 181 VEYATIGKVIDLGVKDPFNMGAAMAPAAVDTIQAHFNDTGRTPKDYDLIVTGDLASVGHP 240 Query: 245 IAKDLLAKEGIPMEQTDFKDCGLMIYDLDKQKYVIAGGSGCGCSATVTYGHILKRMKKGE 304 +AK+LL ++G+PM++T FKDCGLM++D++KQK V AGGSGCGCSA VTYGHILK++ G+ Sbjct: 241 LAKELLIRDGVPMDETTFKDCGLMVFDVEKQK-VQAGGSGCGCSAVVTYGHILKKIANGD 299 Query: 305 LKRVLIVATGALLSPLSYQQGETIPCVAHAVALEMG 340 LKRVL+VATGALLSPLS+QQGE+IPC+AHAVA+ G Sbjct: 300 LKRVLVVATGALLSPLSFQQGESIPCIAHAVAISGG 335 >A6TVT6.1 A6TVT6_ALKMQ Stage V sporulation AD family protein Length = 334 Score = 443 bits (1140), Expect = e-122, Method: Compositional matrix adjust. Identities = 208/335 (62%), Positives = 264/335 (78%), Gaps = 4/335 (1%) Query: 5 GSQTWQYTTRPVILGSSAVVGPEEGEGPLTSDFDFIYDSLKMGEKTWEKAERTLFEKASK 64 G QTW + ++P I+ S+AV GP E +GPL DFD +++ + +G+ ++EKAER L E+A + Sbjct: 4 GRQTWIFDSKPTIVASAAVGGPFEAQGPLREDFDILHEDIWLGQDSFEKAERKLLEQACE 63 Query: 65 LALINASLEQEQLEFFVGGDLMNQIISSSFAARQLGVPYLGVFGACSTSMESLAIASMIV 124 +A+ A +++E + FF+ GDLMNQI SSSFAAR LG+PYLGVFGACS+SMESLA+A++IV Sbjct: 64 IAIDKAGMKKEDIHFFLSGDLMNQITSSSFAARTLGMPYLGVFGACSSSMESLALAALIV 123 Query: 125 DSGGGKYALAGTSSHNCTVEKQFRYPTEYGSQKPPTAQYTVTGSGCAVIGRDDGTSAGPH 184 DSG GK ALA SSHN VEKQFRYPTEYG+QKPPTAQ+TVTG+G AV+ RD GP Sbjct: 124 DSGAGKNALAAASSHNAAVEKQFRYPTEYGAQKPPTAQWTVTGAGAAVVARD---GDGPE 180 Query: 185 VVMATMGRIMDLGLKDPFNMGXXXXXXXXDTITAHFRDTALSPGHYDLIVTGDLASVGLP 244 ++ AT+GR++D+G+ DPFNMG DTI AHFRDT L P +YDLI+TGDL +VG Sbjct: 181 ILWATIGRVVDMGISDPFNMGAAMAPAAVDTIEAHFRDTKLDPSYYDLIITGDLGTVGHH 240 Query: 245 IAKDLLAKEGIPMEQTDFKDCGLMIYDLDKQKYVIAGGSGCGCSATVTYGHILKRMKKGE 304 IA DLL + G+ + + F DCGL+IYD +KQ VIAGGSGC SATVTYGH++KRM KGE Sbjct: 241 IAGDLLQEHGLNISKERFSDCGLLIYDREKQP-VIAGGSGCASSATVTYGHLMKRMGKGE 299 Query: 305 LKRVLIVATGALLSPLSYQQGETIPCVAHAVALEM 339 LKR+L+VATGALLSP+SYQQ E+IPC+AHAVA+EM Sbjct: 300 LKRILVVATGALLSPMSYQQKESIPCIAHAVAIEM 334 >B1DBI9.1 B1DBI9_9BACL Stage V sporulation protein AD Length = 339 Score = 440 bits (1132), Expect = e-122, Method: Compositional matrix adjust. Identities = 200/334 (59%), Positives = 259/334 (77%), Gaps = 4/334 (1%) Query: 5 GSQTWQYTTRPVILGSSAVVGPEEGEGPLTSDFDFIYDSLKMGEKTWEKAERTLFEKASK 64 G Q+W + PVI G++A+VGP EG+GPL DFD ++ L++G+ +WEKAERTL E+A+ Sbjct: 4 GHQSWLFENHPVIAGTAAIVGPFEGQGPLAHDFDIVHGDLRLGQDSWEKAERTLLEEAAN 63 Query: 65 LALINASLEQEQLEFFVGGDLMNQIISSSFAARQLGVPYLGVFGACSTSMESLAIASMIV 124 LA+ A + +E + F VGGDLMNQIISS+FAAR L +PY+GVFGACSTSMESLAIA+ +V Sbjct: 64 LAIEKAQITKESINFLVGGDLMNQIISSTFAARTLQIPYIGVFGACSTSMESLAIAANLV 123 Query: 125 DSGGGKYALAGTSSHNCTVEKQFRYPTEYGSQKPPTAQYTVTGSGCAVIGRDDGTSAGPH 184 +S +Y ++ SHN T EKQFRYPTEYGSQKPPTAQY+VTG+G +V+ + GP+ Sbjct: 124 NSRSAQYVMSSACSHNATAEKQFRYPTEYGSQKPPTAQYSVTGAGVSVLTQQGD---GPY 180 Query: 185 VVMATMGRIMDLGLKDPFNMGXXXXXXXXDTITAHFRDTALSPGHYDLIVTGDLASVGLP 244 V ATMGR++D+G+ DPFNMG DTI AHFRD + PG+YDLIVTGDL+ VG Sbjct: 181 VTSATMGRVVDMGISDPFNMGAAMAPAAVDTIQAHFRDFRIEPGYYDLIVTGDLSKVGYA 240 Query: 245 IAKDLLAKEGIPMEQTDFKDCGLMIYDLDKQKYVIAGGSGCGCSATVTYGHILKRMKKGE 304 IA +L K P+ QT +KDCG+M+Y+ +KQ+ V AG SG GCSATVTYGH+L+RM++ E Sbjct: 241 IANELFEKHNFPIHQTVYKDCGMMMYNYEKQQ-VQAGASGAGCSATVTYGHLLRRMREKE 299 Query: 305 LKRVLIVATGALLSPLSYQQGETIPCVAHAVALE 338 L R+L+VATGALLSP+S+QQGETIPC+AHAV++E Sbjct: 300 LNRILVVATGALLSPISFQQGETIPCIAHAVSIE 333 >B0TIA6.1 B0TIA6_HELMI Stage v sporulation protein ad Length = 336 Score = 437 bits (1123), Expect = e-121, Method: Compositional matrix adjust. Identities = 205/338 (60%), Positives = 262/338 (77%), Gaps = 3/338 (0%) Query: 3 QLGSQTWQYTTRPVILGSSAVVGPEEGEGPLTSDFDFIYDSLKMGEKTWEKAERTLFEKA 62 Q G Q+W++ +PVI+ S+AV GP E GPL+ DFD ++ L +G+ ++EKAER L E+A Sbjct: 2 QQGHQSWRFDNKPVIVASAAVGGPFEARGPLSKDFDLLHGDLWLGQASFEKAERKLLEEA 61 Query: 63 SKLALINASLEQEQLEFFVGGDLMNQIISSSFAARQLGVPYLGVFGACSTSMESLAIASM 122 ++A+ A LE++ ++FF+ GDLMNQI +SSFAAR + VPYLG+FGAC+TSME LA+AS+ Sbjct: 62 CEVAIGKAGLEKKDIDFFLCGDLMNQITASSFAARTMAVPYLGLFGACATSMEGLALASL 121 Query: 123 IVDSGGGKYALAGTSSHNCTVEKQFRYPTEYGSQKPPTAQYTVTGSGCAVIGRDDGTSAG 182 +V+S G+Y L GTSSHN T EKQFRYPTEYG QKPPTAQ+TVTG+G AV+ + G Sbjct: 122 LVNSRAGRYVLCGTSSHNATAEKQFRYPTEYGGQKPPTAQWTVTGAGVAVVAPE---GKG 178 Query: 183 PHVVMATMGRIMDLGLKDPFNMGXXXXXXXXDTITAHFRDTALSPGHYDLIVTGDLASVG 242 P V AT+GRI+D+G+ DPFNMG DTITAHFRD + YDLI TGDL VG Sbjct: 179 PRVTGATIGRIVDMGITDPFNMGAAMAPAAVDTITAHFRDFGIFHEEYDLIATGDLGKVG 238 Query: 243 LPIAKDLLAKEGIPMEQTDFKDCGLMIYDLDKQKYVIAGGSGCGCSATVTYGHILKRMKK 302 IAKDL AK G+ + + F DCGL+IYDLD+Q+ VIAGGSGCGCSATVTYGH+L RMK+ Sbjct: 239 HHIAKDLFAKHGMALPEEKFVDCGLLIYDLDRQEEVIAGGSGCGCSATVTYGHLLNRMKQ 298 Query: 303 GELKRVLIVATGALLSPLSYQQGETIPCVAHAVALEMG 340 GEL+++LIVATGALLSP++YQQ ++IPC+AHAVA+E G Sbjct: 299 GELRKILIVATGALLSPITYQQNDSIPCIAHAVAIERG 336 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,048,670,226 Number of extensions: 81684053 Number of successful extensions: 202868 Number of sequences better than 1.0e-05: 303 Number of HSP's gapped: 202115 Number of HSP's successfully gapped: 303 Length of query: 344 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 208 Effective length of database: 1,779,438,163 Effective search space: 370123137904 Effective search space used: 370123137904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)