BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0011.p (727 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFX7.1 B3KFX7_9BACI Transglutaminase domain protein 518 e-145 B1DBJ7.1 B1DBJ7_9BACL Transglutaminase domain protein 331 2e-88 A7Z234.1 A7Z234_BACA2 YebA 167 4e-39 C0ZK56.1 C0ZK56_BREBN Conserved hypothetical membrane protein 163 6e-38 C2RMB8.1 C2RMB8_BACCE Membrane-bound protease 162 8e-38 >B3KFX7.1 B3KFX7_9BACI Transglutaminase domain protein Length = 746 Score = 518 bits (1334), Expect = e-145, Method: Compositional matrix adjust. Identities = 258/566 (45%), Positives = 354/566 (62%), Gaps = 12/566 (2%) Query: 2 VIARQWVSYTEPYWXXXXXXXXXXXXXXXXXIEILVPVKMIYRLLLEAVTGVYLTYRMIL 61 ++ QWVS+TE W E L+ +K YR++L+ + +Y Y ++ Sbjct: 24 IMGMQWVSFTETIWLSETTAIVIAALTITAVTEALLQIKQGYRVVLQFLLILYCVYSLLK 83 Query: 62 HYGTYVPDPWLPGLRERLPDIMSHMTPYIWFALGAXXXXXXXXXXXXXKPRILMFTGMNI 121 G VP + G L + + ++ PY+WFA+ A K R+L+ G+++ Sbjct: 84 TSGNPVP---VFGAESFLTEELLYLFPYLWFAIAAWAMFLFLARWAKTKGRVLLVVGIHV 140 Query: 122 AAFAALDSFTSSILWQEVAWTVFAGMGWLVTQHLRSFQLHYPRGWTQLLKYPFKIAVNVA 181 +FA LDSFT +LW E+AW V AGMGWLV+ H F+ +P+GW L KYPFKI N+ Sbjct: 141 ISFAILDSFTKIVLWDEIAWVVGAGMGWLVSSHFNRFRRRFPQGWVNLSKYPFKIIANIL 200 Query: 182 IIFSLVIITGVNMPEVRPTLTDPYTAWQEWNXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 241 +IFSL+I+ GVNMP V+PTLTDPYTAW+EWN Sbjct: 201 VIFSLIIVAGVNMPHVKPTLTDPYTAWREWNGVPLSGVGTSGTGTLIESASESMSGYGRE 260 Query: 242 XXXXXXXXLGGGFNFDYSPVMTVTADLRTYMRGETRSVYSGTGWSDDNPFTRGDLKGGVV 301 LGGGFNFDYSPVM++T++ R+Y RGETR+VYSGTGW+D +R G + Sbjct: 261 DND-----LGGGFNFDYSPVMSITSEERSYWRGETRAVYSGTGWAD-GASSRRSGTGVDI 314 Query: 302 GQPLEQNKASKVQTRELKQTVKILGGNSYPVLFGGLSISKVNSVNGADTSSSLLWRSEGS 361 G L + V T++L+Q+V +L YPVLFG S+ ++ S++ L W +EG+ Sbjct: 315 GDSLPGEAGTSVPTKKLEQSVTMLSDTVYPVLFGAYSVRQLESLDEDIDPGRLSWVTEGA 374 Query: 362 ELLWESVENSRAYPREYVLTSQVPVIPIQELNKQTYEQLYGGLDIDQQYLQLPGNFPERV 421 E+ + V ++ YP+ Y LTS+VPVIPI EL+ +TY++LY G D+ Y+Q+P NFP+RV Sbjct: 375 EMHLDDVRDAD-YPKSYTLTSEVPVIPIDELSARTYDELYSG-SFDEAYVQMPRNFPDRV 432 Query: 422 RELAEKVTAQGKTPYEKIALLQQYLQQSFPYTNQPDLSRRKSKDLVESFLFEIMEGYCDY 481 +LAE++TA G TPYEK+ LLQQYL ++ YTN PDLS +KS D V++FLFEIMEGYCDY Sbjct: 433 SDLAEEITADGDTPYEKVMLLQQYLTSNYTYTNTPDLSLKKSDDFVDAFLFEIMEGYCDY 492 Query: 482 YSTALVTMARSLDIPARWVKGYAPGEQAELPDNLAVQQGGAVLNNNYTITNADAHSWAEV 541 +ST++V M RSLDIPARWVKGYAPG + + V ++ +NYT+TNADAHSW EV Sbjct: 493 FSTSMVMMTRSLDIPARWVKGYAPGRMTA-SEYVPVDGQTSIEPDNYTVTNADAHSWVEV 551 Query: 542 YFGDYGWIPVEATPGFTAPLLAQSED 567 YFG+YGW+P+EATPGF+ P+L D Sbjct: 552 YFGEYGWVPIEATPGFSMPILTADAD 577 Score = 100 bits (249), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 46/89 (51%), Positives = 65/89 (73%) Query: 636 GQPLSAVQKVEAETARWVRYARRKGMLKEEHETLRESVDRWSHERPAASGNLTALLRMFE 695 G+PL+A KV AET RW+R +RKG++++EHETLRESV RW++ P L LL+ FE Sbjct: 646 GKPLTAADKVIAETERWIRSVQRKGLVRQEHETLRESVRRWAYAHPELEAELRPLLQQFE 705 Query: 696 RAKYSPEVIEDKDWRSVYTEALRLRKSIK 724 A+YSP ++++ WRSV +A +L+KSIK Sbjct: 706 AARYSPAEVKEEQWRSVQQDAEKLKKSIK 734 >B1DBJ7.1 B1DBJ7_9BACL Transglutaminase domain protein Length = 756 Score = 331 bits (848), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 191/494 (38%), Positives = 266/494 (53%), Gaps = 30/494 (6%) Query: 85 HMTPYIWFALGAXXXXXXXXXXXXXKPRILMFTGMNIAAFAALDSFTSSILWQEVAWTVF 144 + P+IW + + R+ F +NI DSFT LW EVAW VF Sbjct: 121 QLYPFIWISGALLVLYILFDMWITTRMRLFGFISVNILVLTIADSFTPIWLWDEVAWIVF 180 Query: 145 AGMGWLVTQHLRSFQLHYPRGWTQLLKYPFKIAVNVAIIFSLVIITGVNMPEVRPTLTDP 204 G+ WLV HL H+P W +L +YP ++ + + I+ S+++ G+ MP V P + DP Sbjct: 181 LGLLWLVANHLHKLASHHPNSWKELTEYPLQLLMPIVIVLSVLMTAGILMPTVSPIIQDP 240 Query: 205 YTAWQEWNXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLGGGFNFDYSPVMTV 264 YT W+E LGGGF+FDYSPVMT+ Sbjct: 241 YTMWKE---SKGETVNVFLGDKGIAAPVDVSSSNTKSGYGRDDSELGGGFDFDYSPVMTI 297 Query: 265 TADLRTYMRGETRSVYSGTGWSDDNPFTRGDLKGGVVGQ----PLEQNKASKVQTRELKQ 320 T + R+Y RGET++ Y+G GW+ ++ R L VG+ PL + + K +T ++ Q Sbjct: 298 TTNHRSYWRGETKTEYTGAGWTVNDETERNRLAS--VGKDWELPLIEGR-EKAETMKVDQ 354 Query: 321 TVKILGGNSYPVLFGGLSISKVNSVNGADTS---SSLLWRSEGSELLWESVENSRAYPRE 377 V++ N YPVLF I+ V+ V+ D S SL W EL+W S + R +P+ Sbjct: 355 QVRMSNNNIYPVLFAASPITAVHWVDDPDASVLPKSLKWAVNEQELIW-SDKPLRRFPQS 413 Query: 378 YVLTSQVPVIPIQELNKQTYEQLYGGLDIDQQYLQLPGNFPERVRELAEKVTAQGKTPYE 437 Y +TS+V ++ + L K T +L + +QYLQLP + P RVR LA +TA Y+ Sbjct: 414 YAVTSEVTILDEEGLRK-TEAELPDPAET-KQYLQLPSSLPGRVRSLAVSLTASAVNDYD 471 Query: 438 KIALLQQYLQQSFPYTNQPDLSRR--KSKDLVESFLFEIMEGYCDYYSTALVTMARSLDI 495 K L++ YL+Q+F YTN+PD SR SKD V+ FLFE+ EGYCDY+STA+ M+RS+ + Sbjct: 472 KAKLIETYLRQNFMYTNEPDKSRLTGDSKDFVDQFLFELKEGYCDYFSTAMAVMSRSIGL 531 Query: 496 PARWVKGYAPGEQAELPDNLAVQQGGAV-----LN----NNYTITNADAHSWAEVYFGDY 546 P RWVKG+APG LP + V G + LN YT+ N+DAHSW E+YF Y Sbjct: 532 PTRWVKGFAPG---VLPASEYVPSAGGIPSEAELNPDGAGTYTVRNSDAHSWVEIYFDGY 588 Query: 547 GWIPVEATPGFTAP 560 GWIP E T GFT P Sbjct: 589 GWIPFEPTSGFTFP 602 >A7Z234.1 A7Z234_BACA2 YebA Length = 735 Score = 167 bits (423), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 136/461 (29%), Positives = 200/461 (43%), Gaps = 45/461 (9%) Query: 112 RILMFTGMNIAAFAALDSFTSSILWQEVAWTVFAG---MGWLVTQHLRSFQLHYPRGWTQ 168 +IL F M +A LD+FT V VF G MG L + +R + H Sbjct: 141 KILFFFIMTVAYITVLDTFTPYDAAYAVIRIVFIGFLMMGLLYMERIRLTE-HIAFKKRL 199 Query: 169 LLKY--PFKIAVNVAIIFSLVIITGVNMPEVRPTLTDPYTAWQEWNXXXXXXXXXXXXXX 226 +LK+ P + + +++F G+ P+ P DP ++ Sbjct: 200 ILKWFLPLMLFICASVVF------GLAAPKSDPNWPDPVPFLKK---------------- 237 Query: 227 XXXXXXXXXXXXXXXXXXXXXXXLGGGFNFDYSPVMTVTADLRTYMRGETRSVYSGTGWS 286 LGG F D +PV T R Y R ET+ Y+G GW Sbjct: 238 -ITNHKEVPAAESKIGYGNHDETLGGPFQQDDTPVFTWKGKERAYFRVETKDTYTGKGWV 296 Query: 287 DDNPFTRGDLKGGVVGQPLEQNKASKVQTRELKQTVKILGGNSYPVLFGGLSISKVNSVN 346 + + L GG V + + +++ VQTR V I Y + + +S + + Sbjct: 297 ETDQGMSYKLNGGNV-EDIWFDRS--VQTRRYTAQVSIDENYLYNHVMYPIGVSNIKPKH 353 Query: 347 GADTSSSLLWRSEGSELLWESVENSRAYPREYVLTSQVPVIPIQELNKQTYEQLYGGLDI 406 + +E + E V N R R Y +T Q PV + +L K ++ Sbjct: 354 SIQLE--MNGNTEQITPVSEQVGNIRNMGR-YTVTFQAPVFKLNDLRKVKVKRYREQNQF 410 Query: 407 DQQYLQLPGNFPERVRELAEKVTAQGKTPYEKIALLQQYLQQS-FPYTNQPDLSRRKSKD 465 + +YLQ+P + P+RVR+LA+++T ++K ++ YL S F Y Q ++D Sbjct: 411 NDRYLQVPDSLPKRVRQLADRLTRDYHNMFDKAKAIEDYLGSSAFSYNTQDVAVPEHNQD 470 Query: 466 LVESFLFEIMEGYCDYYSTALVTMARSLDIPARWVKGYAPGEQAELPDNLAVQQGGAVLN 525 V+ FLFE GYCD +S+A+ + RS IPARWVKGY GE D G + Sbjct: 471 YVDQFLFETKRGYCDNFSSAMAVLLRSAGIPARWVKGYTSGEYKGAGD-----HNGHI-- 523 Query: 526 NNYTITNADAHSWAEVYFGDYGWIPVEATPGFTAPLLAQSE 566 Y +TN +AHSW EVYF D GW+ E T GFT P SE Sbjct: 524 --YEVTNNNAHSWVEVYFPDQGWVTFEPTKGFTNPTEFTSE 562 >C0ZK56.1 C0ZK56_BREBN Conserved hypothetical membrane protein Length = 748 Score = 163 bits (412), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 105/337 (31%), Positives = 168/337 (49%), Gaps = 46/337 (13%) Query: 250 LGGGFNFDYSPVMTVTADLRTYMRGETRSVYSGTGWSDDNPFTRGDLKGGVVGQPL---- 305 LGG F D + V + +Y RG+++ VY+G GW +GD + + P Sbjct: 262 LGGPFLQDNTLVFIAKTNEHSYWRGDSKDVYTGVGWE------KGDREYESILDPQTYTW 315 Query: 306 EQNKASKVQTRELKQTVKILGGNSYPVLFGGLSISKVN-------SVNGADTSSSLLWRS 358 E ++T++++ T++ G +P +F + KV+ +V + L R+ Sbjct: 316 ENTLFQGLETKKVQATLEFKGPQQFPTVFYQGQLKKVSNYAPPEATVVYDKMNQQLEVRA 375 Query: 359 EGSELLWESVENSRA---------YPREYVLTSQVPVIPIQELNKQ--TYEQLYGGLDID 407 L+ S +N R +Y + ++VP++ + + Y DI Sbjct: 376 GKINLVQLSEKNGRPEYGPNPLLLKLNQYKVETEVPIVSEKAVTNAGTNYPD-----DIK 430 Query: 408 QQYLQLPGNFPERVRELAEKVTAQGKTPYEKIALLQQYLQQS--FPYTNQPDLSRRKSKD 465 ++YLQ+P + P RVRELA +T KTPY+K+ ++ YL+ S + Y + + +D Sbjct: 431 ERYLQVPASVPPRVRELAATITKDAKTPYDKVRAIENYLRSSGKYKYETKDVPVAPEGQD 490 Query: 466 LVESFLFEIMEGYCDYYSTALVTMARSLDIPARWVKGYAPGEQAELPDNLAVQQGGAVLN 525 V+ FLF+ + GYCD++ST++ M R+LDIP RWVKG+APGE+ G N Sbjct: 491 FVDHFLFDSLRGYCDHFSTSMAIMLRTLDIPTRWVKGFAPGERV----------GTDAFN 540 Query: 526 -NNYTITNADAHSWAEVYFGDYGWIPVEATPGFTAPL 561 + N DAHSW EVYF GW+P EAT F +P+ Sbjct: 541 YETVEVRNKDAHSWVEVYFPGVGWVPFEATSTFMSPV 577 >C2RMB8.1 C2RMB8_BACCE Membrane-bound protease Length = 596 Score = 162 bits (411), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 114/314 (36%), Positives = 158/314 (50%), Gaps = 21/314 (6%) Query: 250 LGGGFNFDYSPVMTVTADLRTYMRGETRSVYSGTGWS-DDNPFTRGDLKGGVVGQPLEQN 308 LGG F D + V T + Y R ET+ Y+G GW +NP V EQN Sbjct: 113 LGGPFKADPTIVFTARTQNKHYWRVETKDFYTGKGWGISENPKKISFKNKNDVVSWYEQN 172 Query: 309 KASKVQTRELKQTVKILGGNSYPVLFGGLSISKVNSVNGADTSSSLLWRSEGSELLWESV 368 +K +T E T++ SYP L + V + +D S S+ SE ++ Sbjct: 173 --TKTETTEATITMQ----KSYPHLTYPAGLVSVEA--SSDVSYSV---DPFSEKIYTMN 221 Query: 369 ENSRAYPREYVLTSQVPVIPIQELNK-QTYEQLYGGLDIDQQYLQLPGNFPERVRELAEK 427 E+S Y +T ++P I+ L +T E +Y QLP + P+RV++LA Sbjct: 222 EDSSTTLHSYKVTYEIPEFSIENLKAVKTNEDQETNPYFMTKYTQLPESLPQRVKDLAVN 281 Query: 428 VTAQGKTPYEKIALLQQYL-QQSFPYTNQPDLSRRKSKDLVESFLFEIMEGYCDYYSTAL 486 +T Y+K+ ++ Y SF Y + L KS+D V+ FLF+ GYC+ +ST++ Sbjct: 282 LTNDKDNRYDKVLAIENYFTDNSFTYESTNVLFPAKSQDYVDQFLFDTKSGYCNNFSTSM 341 Query: 487 VTMARSLDIPARWVKGYAPGEQAELPDNLAVQQGGAVLNNNYTITNADAHSWAEVYFGDY 546 + + RS IPARWVKGY G L + LA +G V YTITN +AHSW EVYF Y Sbjct: 342 IVLLRSAGIPARWVKGYTEG---TLDNTLASAEGADV----YTITNDNAHSWVEVYFPGY 394 Query: 547 GWIPVEATPGFTAP 560 GWIP E T GFT P Sbjct: 395 GWIPFEPTKGFTNP 408 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,795,367,695 Number of extensions: 144165976 Number of successful extensions: 390517 Number of sequences better than 1.0e-05: 435 Number of HSP's gapped: 389852 Number of HSP's successfully gapped: 438 Length of query: 727 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 584 Effective length of database: 1,713,491,058 Effective search space: 1000678777872 Effective search space used: 1000678777872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 135 (56.6 bits)