BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0009.p (375 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFX9.1 B3KFX9_9BACI Nitric-oxide synthase EC=1.14.13.39 583 e-165 B1DBJ9.1 B1DBJ9_9BACL Nitric-oxide synthase EC=1.14.13.39 520 e-146 Q2B1B3.1 Q2B1B3_9BACI GTP-binding protein 399 e-109 Q65H33.1 Q65H33_BACLD YqeH 395 e-108 A7GT29.1 A7GT29_BACCN Nitric-oxide synthase EC=1.14.13.39 394 e-108 >B3KFX9.1 B3KFX9_9BACI Nitric-oxide synthase EC=1.14.13.39 Length = 375 Score = 583 bits (1504), Expect = e-165, Method: Compositional matrix adjust. Identities = 287/365 (78%), Positives = 324/365 (88%) Query: 11 EKCSGCGIKLQTTDKDLPGYIPGVAFEREPVICQRCFRIKNYNEASSVSVDQDEFLHLLS 70 +KCSGCGI LQ+ +D PGY+P A++R+PVICQRCFRIKNYNEASSV+VDQDEFL LL Sbjct: 11 KKCSGCGIPLQSAAQDKPGYLPEKAWDRDPVICQRCFRIKNYNEASSVAVDQDEFLRLLG 70 Query: 71 GVGEKNALVIHIVDLFDFEGSLISGLQRFVGNNPVILAVNKSDLLPKVTNWNKLRNWMQQ 130 +G KNALVIHIVDLFDFEGSLISGLQRFVGNNPVILAVNK+DLLPKVTNWNK+ NW+Q+ Sbjct: 71 QIGGKNALVIHIVDLFDFEGSLISGLQRFVGNNPVILAVNKTDLLPKVTNWNKVLNWVQK 130 Query: 131 RCKEHGLRTAEIVLCSAKRNQGFDRLLEAVSELRGQRDVYVVGATNVGKSTLINRLISDY 190 +CKEHGLRT +IVLCSAK+NQGF+RLLE V+ RG RDVYVVGATNVGKS+LINRLI DY Sbjct: 131 QCKEHGLRTEDIVLCSAKKNQGFERLLETVAHYRGDRDVYVVGATNVGKSSLINRLIRDY 190 Query: 191 SDLEQELTTSRYPGTTLDTVKIPLDDGHYIIDTPGIVYPWRYSELVERKDLGAVMPENPL 250 SDL+QELT SRYPGTTLD V IPLDDG YIIDTPGIVYPWRYSELV R+DLG VMPENPL Sbjct: 191 SDLDQELTVSRYPGTTLDMVNIPLDDGRYIIDTPGIVYPWRYSELVSREDLGTVMPENPL 250 Query: 251 KPAVYQLNEGQSLFFGGLGRFDFVKGERQSFTCYISGTLKIHRTKLERADSLYQEHRGEL 310 KP VYQLNEGQ+LFFGG GRFDF++GE QSFTC+ISG L IHRTKLERADSL+ EH GEL Sbjct: 251 KPMVYQLNEGQTLFFGGFGRFDFLQGEHQSFTCFISGRLGIHRTKLERADSLFAEHAGEL 310 Query: 311 LSPPGTADMDKLPQWQRHEFRIGRNSQSDLFISGLGWIKVNGTGGAVVAVHVPKGIKVLT 370 LSPP +++LP+W RHE RI + ++ D+FISGLGWIK+NG GA+VAVHVPKGIKVL+ Sbjct: 311 LSPPTKERLEELPEWTRHEIRIPKGARQDVFISGLGWIKINGEQGALVAVHVPKGIKVLS 370 Query: 371 RPSLI 375 RPSLI Sbjct: 371 RPSLI 375 >B1DBJ9.1 B1DBJ9_9BACL Nitric-oxide synthase EC=1.14.13.39 Length = 374 Score = 520 bits (1340), Expect = e-146, Method: Compositional matrix adjust. Identities = 241/363 (66%), Positives = 291/363 (80%) Query: 13 CSGCGIKLQTTDKDLPGYIPGVAFEREPVICQRCFRIKNYNEASSVSVDQDEFLHLLSGV 72 C+GCG+KLQT + GY+P A REPVICQRCFRI+NYNEASS++VDQD+FL LL + Sbjct: 12 CAGCGVKLQTEHPEESGYLPEAALSREPVICQRCFRIRNYNEASSITVDQDDFLRLLGSI 71 Query: 73 GEKNALVIHIVDLFDFEGSLISGLQRFVGNNPVILAVNKSDLLPKVTNWNKLRNWMQQRC 132 G ++LV+HIVDLFDFEGSLISGLQRFVGNNPV+L VNK DLLP+ N N+LRNW+Q++ Sbjct: 72 GSTDSLVVHIVDLFDFEGSLISGLQRFVGNNPVVLVVNKVDLLPRSVNLNRLRNWVQKQA 131 Query: 133 KEHGLRTAEIVLCSAKRNQGFDRLLEAVSELRGQRDVYVVGATNVGKSTLINRLISDYSD 192 K GLRT +IVLCSAKRN GF+ ++EA+ R RDVYVVGATNVGKSTLINRLI DYSD Sbjct: 132 KAEGLRTVDIVLCSAKRNIGFEHVIEAIERHRKDRDVYVVGATNVGKSTLINRLIHDYSD 191 Query: 193 LEQELTTSRYPGTTLDTVKIPLDDGHYIIDTPGIVYPWRYSELVERKDLGAVMPENPLKP 252 +E+ELTTSRYPGTTLD V IPLDDG IIDTPGIVYP R +E+V R L A++P+ P+KP Sbjct: 192 MERELTTSRYPGTTLDAVHIPLDDGKSIIDTPGIVYPTRMTEIVPRGFLQALLPDKPIKP 251 Query: 253 AVYQLNEGQSLFFGGLGRFDFVKGERQSFTCYISGTLKIHRTKLERADSLYQEHRGELLS 312 VYQLN+ QSLF L RFDFV+GERQSFT YIS L +HRTKLERA+ LY +HRGELL Sbjct: 252 LVYQLNDQQSLFINSLVRFDFVEGERQSFTFYISNALHVHRTKLERAEELYADHRGELLG 311 Query: 313 PPGTADMDKLPQWQRHEFRIGRNSQSDLFISGLGWIKVNGTGGAVVAVHVPKGIKVLTRP 372 P ++D++P W RH RI R ++ D+F+SG+GWI+ NGT GA+V ++ PKGIKVL R Sbjct: 312 GPSREELDEMPAWTRHSIRIPRGAKKDIFVSGMGWIQANGTSGAIVDIYAPKGIKVLLRD 371 Query: 373 SLI 375 SL+ Sbjct: 372 SLV 374 >Q2B1B3.1 Q2B1B3_9BACI GTP-binding protein Length = 367 Score = 399 bits (1024), Expect = e-109, Method: Compositional matrix adjust. Identities = 182/363 (50%), Positives = 253/363 (69%), Gaps = 2/363 (0%) Query: 13 CSGCGIKLQTTDKDLPGYIPGVAFEREPVICQRCFRIKNYNEASSVSVDQDEFLHLLSGV 72 C GCG+K+QT + + GY P A E+E ++CQRCFR+K+YNE VS+ D+FL +L+ + Sbjct: 7 CIGCGVKVQTENPEELGYAPKSALEKEAIVCQRCFRLKHYNEVQDVSLTDDDFLKILNEI 66 Query: 73 GEKNALVIHIVDLFDFEGSLISGLQRFVGNNPVILAVNKSDLLPKVTNWNKLRNWMQQRC 132 G +AL++ IVD+FDF GS + GL RF GNN V+L NK+DLLPK NKL NWM+Q Sbjct: 67 GRSDALIVKIVDIFDFNGSWLPGLHRFTGNNKVLLVGNKADLLPKSVKRNKLINWMKQES 126 Query: 133 KEHGLRTAEIVLCSAKRNQGFDRLLEAVSELRGQRDVYVVGATNVGKSTLINRLISDYSD 192 K GLR ++ L SA + Q + A+ E R +DVYVVG TNVGKST INR++ + + Sbjct: 127 KNLGLRPEDVFLVSAAKGQSIQEVASAIDEYREGKDVYVVGCTNVGKSTFINRILKEVTG 186 Query: 193 LEQELTTSRYPGTTLDTVKIPLDDGHYIIDTPGIVYPWRYSELVERKDLGAVMPENPLKP 252 +TTS +PGTTLD ++IPL+DG ++DTPGI+ + + V+++D +MP+ +KP Sbjct: 187 EGDIITTSHFPGTTLDIIEIPLEDGKALVDTPGIINHHQMAHFVDKRDFKVIMPKKEIKP 246 Query: 253 AVYQLNEGQSLFFGGLGRFDFVKGERQSFTCYISGTLKIHRTKLERADSLYQEHRGELLS 312 VYQLNEGQ+LFFGGL RFDF+ G RQ+F C++S + IHRTKLE AD LY++H GE+L+ Sbjct: 247 KVYQLNEGQTLFFGGLARFDFISGSRQAFICHVSNEINIHRTKLENADELYEKHAGEMLT 306 Query: 313 PPGTADMDKLPQWQRHEFRIGRNSQSDLFISGLGWIKVNGTGGAVVAVHVPKGIKVLTRP 372 PP MD+ P+ RHEF + + ++D+ ISGLGW+ V+ G VA +VPKG++ + R Sbjct: 307 PPRPEQMDEFPELVRHEFTV-KEGKTDIVISGLGWVTVSEPGTK-VAAYVPKGVQAMVRK 364 Query: 373 SLI 375 SLI Sbjct: 365 SLI 367 >Q65H33.1 Q65H33_BACLD YqeH Length = 366 Score = 395 bits (1016), Expect = e-108, Method: Compositional matrix adjust. Identities = 186/363 (51%), Positives = 248/363 (68%), Gaps = 2/363 (0%) Query: 13 CSGCGIKLQTTDKDLPGYIPGVAFEREPVICQRCFRIKNYNEASSVSVDQDEFLHLLSGV 72 C GCG+ +QT DK+ GY P + +E VICQRCFR+KNYNE VS+ D+FL +L G+ Sbjct: 6 CIGCGVAIQTEDKNKLGYAPEASLLKEDVICQRCFRLKNYNEIQDVSLTDDDFLKILHGI 65 Query: 73 GEKNALVIHIVDLFDFEGSLISGLQRFVGNNPVILAVNKSDLLPKVTNWNKLRNWMQQRC 132 GE ++L++ IVD+FDF GS I+GL RFVG NP++L NK+D+LPK +L NWM++ Sbjct: 66 GETDSLIVKIVDIFDFNGSWINGLNRFVGGNPILLIGNKADILPKSVKRERLVNWMKREA 125 Query: 133 KEHGLRTAEIVLCSAKRNQGFDRLLEAVSELRGQRDVYVVGATNVGKSTLINRLISDYSD 192 KE GLR +++L SA R QG ++A+ R +DVYVVG TNVGKST INR+I + S Sbjct: 126 KELGLRPVDVLLVSAGRGQGMKDAIDAIEHYRQGKDVYVVGCTNVGKSTFINRIIKEVSG 185 Query: 193 LEQELTTSRYPGTTLDTVKIPLDDGHYIIDTPGIVYPWRYSELVERKDLGAVMPENPLKP 252 + +TTS+YPGTTLD ++IPLDDG + DTPGI+ + + V ++DL + P+ LKP Sbjct: 186 EDDIITTSQYPGTTLDAIEIPLDDGSAMYDTPGIINHHQMAHYVAKRDLKILTPKKELKP 245 Query: 253 AVYQLNEGQSLFFGGLGRFDFVKGERQSFTCYISGTLKIHRTKLERADSLYQEHRGELLS 312 +QLNE Q+LFFGGL RFD+ G R SF CY+ LKIHRTKLE AD LY++H GE+L+ Sbjct: 246 RTFQLNEEQTLFFGGLSRFDYASGGRSSFVCYMPNELKIHRTKLENADQLYEKHAGEMLA 305 Query: 313 PPGTADMDKLPQWQRHEFRIGRNSQSDLFISGLGWIKVNGTGGAVVAVHVPKGIKVLTRP 372 PP D+D P +H F I + ++D+ SGLGW+ V G +VA H PKG+ V R Sbjct: 306 PPAKEDLDSFPALVKHTFTI-KEPKTDVVFSGLGWVTVQGANQKIVA-HAPKGVHVFLRR 363 Query: 373 SLI 375 SLI Sbjct: 364 SLI 366 >A7GT29.1 A7GT29_BACCN Nitric-oxide synthase EC=1.14.13.39 Length = 368 Score = 394 bits (1011), Expect = e-108, Method: Compositional matrix adjust. Identities = 184/365 (50%), Positives = 256/365 (70%), Gaps = 3/365 (0%) Query: 12 KCSGCGIKLQTTDKDLPGYIPGVAFEREPVICQRCFRIKNYNEASSVSVDQDEFLHLLSG 71 KC GCG+++QT +K GY P + ++E VICQRCFR+KNYNE VS+ D+FL +L+G Sbjct: 6 KCIGCGVEIQTENKSEVGYAPASSLQKEQVICQRCFRLKNYNEIQDVSLTDDDFLRILNG 65 Query: 72 VGEKNALVIHIVDLFDFEGSLISGLQRFVGNNPVILAVNKSDLLPKVTNWNKLRNWMQQR 131 +G+ ALV+ IVD+FDF GS + GL RFVGNN V+L NK+DL+PK +K+++WM+ Sbjct: 66 IGQSEALVVKIVDIFDFNGSWLPGLHRFVGNNKVLLVGNKADLIPKSVKHDKMKHWMRYS 125 Query: 132 CKEHGLRTAEIVLCSAKRNQGFDRLLEAVSELRGQRDVYVVGATNVGKSTLINRLISDYS 191 K+ GL+ ++ L SA + QG L EA+ + R +DVYVVG TNVGKST INR+I ++S Sbjct: 126 AKQLGLKAEDVFLISAAKGQGIQELAEAIEKYRNGKDVYVVGCTNVGKSTFINRMIKEFS 185 Query: 192 -DLEQELTTSRYPGTTLDTVKIPLDDGHYIIDTPGIVYPWRYSELVERKDLGAVMPENPL 250 + E +TTS +PGTTLD + IPLDD + DTPGI+ + + V ++ L + P + Sbjct: 186 EETENVITTSHFPGTTLDLIDIPLDDTSSLYDTPGIINHHQMAHYVGKQSLKIITPTKEI 245 Query: 251 KPAVYQLNEGQSLFFGGLGRFDFVKGERQSFTCYISGTLKIHRTKLERADSLYQEHRGEL 310 KP V+QLNE Q+LFF GL RFD+V G R+SFTCY S L IHRTKLE+AD L+++H GEL Sbjct: 246 KPMVFQLNEEQTLFFSGLARFDYVSGGRRSFTCYFSNRLTIHRTKLEKADQLHEKHAGEL 305 Query: 311 LSPPGTADMDKLPQWQRHEFRIGRNSQSDLFISGLGWIKVNGTGGAVVAVHVPKGIKVLT 370 L+PP +++ +P++ ++EF I R ++D+ SGLGW+ VN G +VA H+PKG+ V Sbjct: 306 LNPPTPEELESMPEFVKYEFNI-REPKTDVVFSGLGWVTVNEPGAKIVA-HIPKGVSVSL 363 Query: 371 RPSLI 375 R SLI Sbjct: 364 RKSLI 368 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.138 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,348,323,017 Number of extensions: 101481911 Number of successful extensions: 331874 Number of sequences better than 1.0e-05: 499 Number of HSP's gapped: 332202 Number of HSP's successfully gapped: 525 Length of query: 375 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 238 Effective length of database: 1,770,017,148 Effective search space: 421264081224 Effective search space used: 421264081224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)