BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0008.p (289 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B1DBK0.1 B1DBK0_9BACL Shikimate 5-dehydrogenase 289 2e-76 B3KFY0.1 B3KFY0_9BACI Shikimate 5-dehydrogenase 287 8e-76 A8VU65.1 A8VU65_9BACI Multiple resistance and pH regulation prot... 217 8e-55 Q5KWX7.1 Q5KWX7_GEOKA Shikimate 5-dehydrogenase EC=1.1.1.25 208 4e-52 C2WCB2.1 C2WCB2_BACCE Shikimate dehydrogenase 204 7e-51 >B1DBK0.1 B1DBK0_9BACL Shikimate 5-dehydrogenase Length = 293 Score = 289 bits (740), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 138/285 (48%), Positives = 185/285 (64%), Gaps = 4/285 (1%) Query: 5 NGQYGNK----DILLGVMGDPIAHTKSPLMHTAALKALGIPGAYVPLHIAPGQLGEAIQA 60 NG GN+ +L GV+GDP+ H+KSP+M A + GI GAY+ HI+P +LGE + Sbjct: 8 NGITGNRIDSQTVLYGVIGDPVRHSKSPIMMNRAFRETGINGAYMAFHISPDKLGEFVAG 67 Query: 61 IRTLGFRGVNVTIPHKVAVMEYIDRIDDSAXXXXXXXXXXXXXXXLTGYNTDGIGYVRSL 120 IR +G RGVNVTIPHK+ +M+Y+D ID A LTGYNTDGIGYVRSL Sbjct: 68 IRAMGIRGVNVTIPHKLDIMKYMDEIDAGAQAIGAVNTIVNDNGRLTGYNTDGIGYVRSL 127 Query: 121 KAEATPDLAGSXXXXXXXXXXXXXXXXXXXXEHPAAVVIANRTVEKSKQLAEAFQGKGTV 180 K EA P+LAG E P+ + IANR+VE++++LA++F+ + Sbjct: 128 KEEAEPELAGKRILVIGAGGASRGIVYALAGEQPSGITIANRSVERAEELAQSFRSLADI 187 Query: 181 NGIGNDEIAAHIADMDIVINTTSVGMYPHIDAMPIDPALLREGMIVSDLIYNPLQTRLLT 240 I ND + A + DIVINTTSVGM+P+ DA PID A L+ G + SDLIYNPL+T+ L Sbjct: 188 RAIANDGLQAACKEADIVINTTSVGMHPNTDATPIDAAWLKPGAVASDLIYNPLRTKFLL 247 Query: 241 ESLQRGCRIHGGLGMFVYQGAYSLEYWSGRSAPVDIMRQTIIDCL 285 ++ + GCR+HGGLGMF+YQGAY+ EYW+G+ AP MR+T++ L Sbjct: 248 DAERHGCRVHGGLGMFIYQGAYAFEYWTGQQAPAAAMRETVLQSL 292 >B3KFY0.1 B3KFY0_9BACI Shikimate 5-dehydrogenase Length = 288 Score = 287 bits (734), Expect = 8e-76, Method: Compositional matrix adjust. Identities = 140/273 (51%), Positives = 177/273 (64%) Query: 13 ILLGVMGDPIAHTKSPLMHTAALKALGIPGAYVPLHIAPGQLGEAIQAIRTLGFRGVNVT 72 +LLGVMGDPI H+KSP MH AA+ ALG+ GAYVPLHI P LG+AIQA++ LGFRGVNVT Sbjct: 12 LLLGVMGDPIGHSKSPAMHDAAINALGLSGAYVPLHIRPQGLGDAIQAVKVLGFRGVNVT 71 Query: 73 IPHKVAVMEYIDRIDDSAXXXXXXXXXXXXXXXLTGYNTDGIGYVRSLKAEATPDLAGSX 132 IPHKV VM+Y+D +D+ A LTGYNTDGIGYVRSLK EA PDL G Sbjct: 72 IPHKVEVMKYLDVVDEGAQRIGAVNTIVNDNGKLTGYNTDGIGYVRSLKDEACPDLKGKR 131 Query: 133 XXXXXXXXXXXXXXXXXXXEHPAAVVIANRTVEKSKQLAEAFQGKGTVNGIGNDEIAAHI 192 E P + I NRT K+ LAE + + G G ++ + Sbjct: 132 IVVIGAGGAARGIIYALIGEGPEKISIVNRTSAKALALAEEWGSLADLRGYGEEQAGEAL 191 Query: 193 ADMDIVINTTSVGMYPHIDAMPIDPALLREGMIVSDLIYNPLQTRLLTESLQRGCRIHGG 252 +D D++INTTSVGM+P + +PI P +REG++VSDLIYNPL+T LL + R C IHGG Sbjct: 192 SDADVIINTTSVGMFPRVSELPIPPESIREGIVVSDLIYNPLKTELLRQGELRRCTIHGG 251 Query: 253 LGMFVYQGAYSLEYWSGRSAPVDIMRQTIIDCL 285 LGMF+ QGAY+ EYW+G +APV M++ ++ Sbjct: 252 LGMFINQGAYAFEYWTGLTAPVQAMKEAVLTSF 284 >A8VU65.1 A8VU65_9BACI Multiple resistance and pH regulation protein F Length = 277 Score = 217 bits (553), Expect = 8e-55, Method: Compositional matrix adjust. Identities = 115/274 (41%), Positives = 155/274 (56%) Query: 14 LLGVMGDPIAHTKSPLMHTAALKALGIPGAYVPLHIAPGQLGEAIQAIRTLGFRGVNVTI 73 + GV GDPI H+ SP MH AAL GI GAY H+ P L +A++ +R LG RGVNVTI Sbjct: 4 IFGVFGDPIEHSMSPAMHEAALSHAGIDGAYHAFHVKPEGLQDAVKGVRALGIRGVNVTI 63 Query: 74 PHKVAVMEYIDRIDDSAXXXXXXXXXXXXXXXLTGYNTDGIGYVRSLKAEATPDLAGSXX 133 PHKV VM+++D ID A LTG+NTDG GY+ L+ T L Sbjct: 64 PHKVDVMKHLDAIDPLAERIGAVNTIVNDDGVLTGFNTDGAGYLEGLRPLLTHPLTDMRV 123 Query: 134 XXXXXXXXXXXXXXXXXXEHPAAVVIANRTVEKSKQLAEAFQGKGTVNGIGNDEIAAHIA 193 + + +ANRT K+ +LA+A + + A + Sbjct: 124 LIIGAGGAARAVAMTLASKEVKELYVANRTETKAGELAKACKPYAASRSLTLSLAQARLM 183 Query: 194 DMDIVINTTSVGMYPHIDAMPIDPALLREGMIVSDLIYNPLQTRLLTESLQRGCRIHGGL 253 + D+VINTTSVGM P++D +PI +L IV+DLIYNP++TRLL + Q+G + GL Sbjct: 184 EFDLVINTTSVGMSPNVDNIPISLEMLGRDTIVTDLIYNPMETRLLKMARQKGAKTQNGL 243 Query: 254 GMFVYQGAYSLEYWSGRSAPVDIMRQTIIDCLGG 287 MFV QG + E W+G++AP DIMRQT++D LGG Sbjct: 244 NMFVLQGGLAFEKWTGKTAPRDIMRQTVLDQLGG 277 >Q5KWX7.1 Q5KWX7_GEOKA Shikimate 5-dehydrogenase EC=1.1.1.25 Length = 276 Score = 208 bits (530), Expect = 4e-52, Method: Compositional matrix adjust. Identities = 109/271 (40%), Positives = 155/271 (57%), Gaps = 3/271 (1%) Query: 16 GVMGDPIAHTKSPLMHTAALKALGIPGAYVPLHIAPGQLGEAIQAIRTLGFRGVNVTIPH 75 G++G P+ H+ SPLMH A LGIP Y + PGQ+G AI +R LG GVNVTIPH Sbjct: 6 GLIGFPVEHSLSPLMHNDAFARLGIPARYHLFSVEPGQVGAAIAGVRALGIAGVNVTIPH 65 Query: 76 KVAVMEYIDRIDDSAXXXXXXXXXXXXXXXLTGYNTDGIGYVRSLKAEATPDLAGSXXXX 135 K+AV+ ++D +D+ A L GYNTDG+GYV++L+ E L G Sbjct: 66 KLAVIPFLDEVDEHARRIGAVNTIINNDGRLVGYNTDGLGYVQALEEEMNITLDGKRILV 125 Query: 136 XXXXXXXXXXXXXXXXEHPAAVVIANRTVEKSKQLA-EAFQGKGTVNGIGNDEIAAHIAD 194 + +ANRTVEK+++L E + + + E +A+ Sbjct: 126 IGAGGGARGIYFSLLSTAAERIDMANRTVEKAERLVREGDERRSAYFSLAEAE--TRLAE 183 Query: 195 MDIVINTTSVGMYPHIDAMPIDPALLREGMIVSDLIYNPLQTRLLTESLQRGCRIHGGLG 254 DI+INTTSVGM+P ++ P+ LR G+IVSD+IYNPL+T+ L E+ RG R+ G+G Sbjct: 184 YDIIINTTSVGMHPRVEVQPLSLERLRPGVIVSDIIYNPLETKWLKEAKARGARVQNGVG 243 Query: 255 MFVYQGAYSLEYWSGRSAPVDIMRQTIIDCL 285 M VYQGA + E W+G+ V+ M+Q +I+ L Sbjct: 244 MLVYQGALAFEKWTGQWPDVNRMKQLVIEAL 274 >C2WCB2.1 C2WCB2_BACCE Shikimate dehydrogenase Length = 277 Score = 204 bits (519), Expect = 7e-51, Method: Compositional matrix adjust. Identities = 106/274 (38%), Positives = 153/274 (55%) Query: 14 LLGVMGDPIAHTKSPLMHTAALKALGIPGAYVPLHIAPGQLGEAIQAIRTLGFRGVNVTI 73 L GV+G+PI H+ SPLMH A + I Y + LGEA++ ++ LG G NVT Sbjct: 4 LYGVIGNPIGHSLSPLMHNDAFENCSIDAHYHAFLVEEMALGEAVKGLKALGVSGFNVTT 63 Query: 74 PHKVAVMEYIDRIDDSAXXXXXXXXXXXXXXXLTGYNTDGIGYVRSLKAEATPDLAGSXX 133 PHKV++M+Y+D ID A L GYNTDGIGYVRSL++ + L Sbjct: 64 PHKVSIMKYLDEIDPLAKQIGAVNTVVQRDEKLIGYNTDGIGYVRSLQSISKDPLDQKRI 123 Query: 134 XXXXXXXXXXXXXXXXXXEHPAAVVIANRTVEKSKQLAEAFQGKGTVNGIGNDEIAAHIA 193 + IANRTVEK+ QL QGK T + + ++ Sbjct: 124 LLLGAGGACRAIYFSLADAGVKEIDIANRTVEKANQLIAGCQGKITSHALSLEQATEKQK 183 Query: 194 DMDIVINTTSVGMYPHIDAMPIDPALLREGMIVSDLIYNPLQTRLLTESLQRGCRIHGGL 253 + DI+I+TT++GMYPH+ + P+ L++G IVSD+IYNP +T+LL ++ +G + G+ Sbjct: 184 NYDIIIHTTTIGMYPHVQSTPLQIQSLKQGTIVSDIIYNPFETKLLQDAKAQGAIVQNGI 243 Query: 254 GMFVYQGAYSLEYWSGRSAPVDIMRQTIIDCLGG 287 MFVYQGA + E W+GR ++ M+Q ++ LGG Sbjct: 244 DMFVYQGALAFEMWTGRIPNIERMKQLVMRKLGG 277 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,527,181,849 Number of extensions: 56650734 Number of successful extensions: 130730 Number of sequences better than 1.0e-05: 1770 Number of HSP's gapped: 127901 Number of HSP's successfully gapped: 2007 Length of query: 289 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 155 Effective length of database: 1,798,280,193 Effective search space: 278733429915 Effective search space used: 278733429915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)