BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0002.p (256 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KFY6.1 B3KFY6_9BACI Methyltransferase type 12 394 e-108 B1DBK7.1 B1DBK7_9BACL Methyltransferase type 12 290 7e-77 C0ZB12.1 C0ZB12_BREBN Putative uncharacterized protein yqeM 213 2e-53 C3B887.1 C3B887_BACMY Methyltransferase 181 6e-44 C3AQT9.1 C3AQT9_BACMY Methyltransferase 181 6e-44 >B3KFY6.1 B3KFY6_9BACI Methyltransferase type 12 Length = 256 Score = 394 bits (1011), Expect = e-108, Method: Compositional matrix adjust. Identities = 185/254 (72%), Positives = 208/254 (81%) Query: 3 SYGKFAYVYDELMADMPYPDWLAFAEAAWSKYGKPLTVAELGCGTGSITIPLAASGYHMT 62 SY KFAYVYD+LM DMPYPDW+ FA AW K+G P +V ELGCGTGSITIPL SG+ +T Sbjct: 3 SYRKFAYVYDQLMEDMPYPDWIRFARTAWEKHGMPKSVVELGCGTGSITIPLVNSGFEVT 62 Query: 63 GIDLSSDMLSVAQMKMERHPQGRRFLREGSVRWVRQNMKEWELPEPVDSVISFCDCLNYV 122 GIDLS+DMLSVA+ K+E PQG R REGSVRWV+Q+M+ WELPE VDSVISFCDC+NY+ Sbjct: 63 GIDLSADMLSVARRKLEGMPQGHRLFREGSVRWVQQDMRSWELPEQVDSVISFCDCVNYL 122 Query: 123 LEERDITSVFECTYAGLKAGGTFLFDVHHPNTLIRYEEEQPFVLDEPAVSYIWTCEMDVP 182 LEE+DI S F TY GLK GGTFLFDVHHPNTL+RY+EEQPFVLDE VSYIWTC++D Sbjct: 123 LEEKDIRSAFARTYNGLKEGGTFLFDVHHPNTLLRYDEEQPFVLDEREVSYIWTCDLDER 182 Query: 183 RREIEHHLSIFAREEGGSGLYRRFEETHIQRAYDPEWMAEELRKAGFSEVKVYADFEWVE 242 R EIEHHLSIFAR++ +YRRFEETHIQR YDPEWM EL KAGF +VK YADFEW E Sbjct: 183 RMEIEHHLSIFARDDQDRTIYRRFEETHIQRGYDPEWMKVELIKAGFRDVKCYADFEWTE 242 Query: 243 ADDSAGRLFYVAVK 256 A A RLFYVAVK Sbjct: 243 AGQDASRLFYVAVK 256 >B1DBK7.1 B1DBK7_9BACL Methyltransferase type 12 Length = 247 Score = 290 bits (742), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 145/248 (58%), Positives = 173/248 (69%), Gaps = 5/248 (2%) Query: 10 VYDELMADMPYPDWLAFAEAAWSKYGKPLTVAELGCGTGSITIPLAASGYHMTGIDLSSD 69 VYD LM DMPY DWL FA +W +YG P TVA+LGCGTGSI IPLA SG + GIDLS+D Sbjct: 4 VYDRLMQDMPYEDWLQFAHDSWERYGMPKTVADLGCGTGSIAIPLALSGLEVYGIDLSAD 63 Query: 70 MLSVAQMK-MERHPQGRRFLREGSVRWVRQNMKEWELPEPVDSVISFCDCLNYVLEERDI 128 ML+VA K E Q R G+V W+ Q+M++W L EPVDSVISFCDC+NY+ EE D+ Sbjct: 64 MLTVASSKWNEASTQMAR--ARGNVTWLEQDMRDWTLAEPVDSVISFCDCVNYLTEEEDV 121 Query: 129 TSVFECTYAGLKAGGTFLFDVHHPNTLIRYEEEQPFVLDEPAVSYIWTCEMDVPRREIEH 188 + F+ Y L+ GG FLFDVH P +RY EEQPFVLDE V+YIWTCE+D R +IEH Sbjct: 122 EAAFKAAYDNLREGGLFLFDVHDPKVFLRYAEEQPFVLDEDEVAYIWTCELDESRLQIEH 181 Query: 189 HLSIFAREEGGSGLYRRFEETHIQRAYDPEWMAEELRKAGFSEVKVYADFEWVEADDSAG 248 HL+IFA E+ SG + RFEE H QRAY WM L++AGF V YADFE A D + Sbjct: 182 HLTIFAEEK--SGKFERFEEMHEQRAYGRGWMEAALKRAGFRTVDCYADFELEPAGDRSE 239 Query: 249 RLFYVAVK 256 RLF+ AVK Sbjct: 240 RLFFAAVK 247 >C0ZB12.1 C0ZB12_BREBN Putative uncharacterized protein yqeM Length = 249 Score = 213 bits (541), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 113/254 (44%), Positives = 154/254 (60%), Gaps = 11/254 (4%) Query: 3 SYGKFAYVYDELMADMPYPDWLAFAEAAWSKYGKPLTVAELGCGTGSITIPLAASGYHMT 62 +Y A VYD LMAD PY WL + E W K KP+ V +LGCGTG+I IPLA GY +T Sbjct: 2 AYAHMAAVYDRLMADTPYDQWLDWVERHWKKGEKPVRVIDLGCGTGTIAIPLAKRGYRVT 61 Query: 63 GIDLSSDMLSVAQMKMERHPQGRRFLREGSVRWVRQNMKEWELPEPVDSVISFCDCLNYV 122 G+DLS++ML++A KM + + V WV Q+M+E LPE D+VIS CD L+Y+ Sbjct: 62 GVDLSTEMLAIAYDKMRQ--------EQVEVAWVEQDMRELSLPE-ADAVISLCDSLSYL 112 Query: 123 LEERDITSVFECTYAGLKAGGTFLFDVHHPNTLIRYEEEQPFVLDEPAVSYIWTCEMDVP 182 EE D+ F+ +A L GG+FLFDVH P ++ ++ F E VSYIW C D Sbjct: 113 TEEADVQETFKRVFAHLAPGGSFLFDVHSPYKMLHIFGDETFTHVEDEVSYIWQCFCDPL 172 Query: 183 RREIEHHLSIFAREEGGSGLYRRFEETHIQRAYDPEWMAEELRKAGFSEVKVYADFEWVE 242 R E+EH L+ F R+ +GLY R EE H QRAY P + L AGF+++ + AD+ + Sbjct: 173 RLEVEHQLTFFIRQ--PNGLYERMEEEHWQRAYQPVQLMRWLTDAGFTDIIITADYSDLP 230 Query: 243 ADDSAGRLFYVAVK 256 +++ RLF+ A K Sbjct: 231 PQEASERLFFSARK 244 >C3B887.1 C3B887_BACMY Methyltransferase Length = 249 Score = 181 bits (459), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 94/254 (37%), Positives = 146/254 (57%), Gaps = 10/254 (3%) Query: 4 YGKFAYVYDELMADMPYPDWLAFAEAAWSKYG-KPLTVAELGCGTGSITIPLAASGYHMT 62 Y +FA +YDELM D+PY W+ F E + + G K + ++ CGTG++T+PL GY M Sbjct: 3 YEQFALLYDELMNDVPYDKWVEFTEESLQQAGMKEAKILDVACGTGNVTLPLVRKGYDMI 62 Query: 63 GIDLSSDMLSVAQMKMERHPQGRRFLREGSVRWVRQNMKEWELPEPVDSVISFCDCLNYV 122 G+DLS +ML+VAQ K+ G + + + +Q+M+E ++P D V FCD LNYV Sbjct: 63 GVDLSEEMLTVAQQKL----GGEGYF----IPFYQQDMRELDVPGEFDCVTIFCDSLNYV 114 Query: 123 LEERDITSVFECTYAGLKAGGTFLFDVHHPNTLIRYEEEQPFVLDEPAVSYIWTCEMDVP 182 L+E I F + L+ G FLFDVH + + + + ++ ++ IW C Sbjct: 115 LQEEGIQETFRRVFHHLRQDGLFLFDVHSLYKIYHVFQNETYTVNGEEIALIWNCFPGEE 174 Query: 183 RREIEHHLSIFAREEGGSGLYRRFEETHIQRAYDPEWMAEELRKAGFSEVKVYADFEWVE 242 +EH LS F ++ G+Y RF+E H+QRAY E + + L +AGF+ ++V DFE +E Sbjct: 175 SDSVEHDLSFFVQDP-EEGVYHRFDECHMQRAYSVELLTKWLEEAGFTVLRVTGDFERME 233 Query: 243 ADDSAGRLFYVAVK 256 + R+F++A K Sbjct: 234 VTEQTERIFFMAKK 247 >C3AQT9.1 C3AQT9_BACMY Methyltransferase Length = 249 Score = 181 bits (459), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 94/254 (37%), Positives = 146/254 (57%), Gaps = 10/254 (3%) Query: 4 YGKFAYVYDELMADMPYPDWLAFAEAAWSKYG-KPLTVAELGCGTGSITIPLAASGYHMT 62 Y +FA +YDELM D+PY W+ F E + + G K + ++ CGTG++T+PL GY M Sbjct: 3 YEQFALLYDELMNDVPYDKWVEFTEESLQQAGMKEAKILDVACGTGNVTLPLVRKGYDMI 62 Query: 63 GIDLSSDMLSVAQMKMERHPQGRRFLREGSVRWVRQNMKEWELPEPVDSVISFCDCLNYV 122 G+DLS +ML+VAQ K+ G + + + +Q+M+E ++P D V FCD LNYV Sbjct: 63 GVDLSEEMLTVAQQKL----GGEGYF----IPFYQQDMRELDVPGEFDCVTIFCDSLNYV 114 Query: 123 LEERDITSVFECTYAGLKAGGTFLFDVHHPNTLIRYEEEQPFVLDEPAVSYIWTCEMDVP 182 L+E I F + L+ G FLFDVH + + + + ++ ++ IW C Sbjct: 115 LQEEGIQETFRRVFHHLRQDGLFLFDVHSLYKIYHVFQNETYTVNGEEIALIWNCFPGEE 174 Query: 183 RREIEHHLSIFAREEGGSGLYRRFEETHIQRAYDPEWMAEELRKAGFSEVKVYADFEWVE 242 +EH LS F ++ G+Y RF+E H+QRAY E + + L +AGF+ ++V DFE +E Sbjct: 175 SDSVEHDLSFFVQDP-EEGVYHRFDECHMQRAYSVELLTKWLEEAGFTVLRVTGDFERME 233 Query: 243 ADDSAGRLFYVAVK 256 + R+F++A K Sbjct: 234 VTEQTERIFFMAKK 247 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,704,249,041 Number of extensions: 71571415 Number of successful extensions: 176819 Number of sequences better than 1.0e-05: 514 Number of HSP's gapped: 176004 Number of HSP's successfully gapped: 514 Length of query: 256 Length of database: 3,060,696,203 Length adjustment: 133 Effective length of query: 123 Effective length of database: 1,807,701,208 Effective search space: 222347248584 Effective search space used: 222347248584 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)