BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0545.0001.p (330 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q9EWV3.2 Q9EWV3_STRCO Putative oxidoreductase 486 e-136 C1WFQ0.1 C1WFQ0_9ACTO Predicted oxidoreductase similar to aryl-a... 471 e-131 Q82GC1.1 Q82GC1_STRAW Putative oxidoreductase 439 e-121 C2AA54.1 C2AA54_THECU Predicted oxidoreductase similar to aryl-a... 400 e-109 Q0C022.1 Q0C022_HYPNA Oxidoreductase, aldo/keto reductase family 396 e-108 >Q9EWV3.2 Q9EWV3_STRCO Putative oxidoreductase Length = 330 Score = 486 bits (1252), Expect = e-136, Method: Compositional matrix adjust. Identities = 239/330 (72%), Positives = 282/330 (85%) Query: 1 MQTRILGQQGLKVSSIGLGTMGMTMAYGPSNENEGIATIRRAYELGVNFFDTAELYGYGN 60 MQ R LGQQGL+VS++GLG MGM++AYGPSN+ +GI+TI RA+ELGV+FFDTAELYG G Sbjct: 1 MQHRTLGQQGLRVSALGLGVMGMSLAYGPSNDEDGISTIHRAHELGVDFFDTAELYGQGT 60 Query: 61 GHNEQLLGKAVKDFRDKVVLATKFGFDMTAEQLGTRFNSRPENIRKVAENSLRYLQTDYI 120 G NE LLG AV DFRD+VVLATKFGFDMT++ LG+ NSRPENIR+VAENSLRYL+TD I Sbjct: 61 GSNETLLGNAVNDFRDEVVLATKFGFDMTSKSLGSGVNSRPENIREVAENSLRYLRTDRI 120 Query: 121 DVFYQHIPDPDVPIEEVAGVVGDLIKEGKVKYFGLSNTGSKTIRRAHAVTPVSVLQTEYS 180 D+FYQHI DPDVP EEVAG VGDLI EGKV+YFGLSN G + IRRAHAVTPV+ LQ EYS Sbjct: 121 DLFYQHISDPDVPAEEVAGTVGDLITEGKVRYFGLSNVGPRYIRRAHAVTPVTALQYEYS 180 Query: 181 IFEREIEDDILPTLNELGIGLVPYSPLGRGFLTDAVKPSHEYPEGDMRRFDERWQGENYI 240 IFERE+ED+ILP + ELGIGLVPYSPLGRGFL+ V+P+ EYPE DMRR+DERWQGENY Sbjct: 181 IFEREVEDEILPVVRELGIGLVPYSPLGRGFLSGVVRPAGEYPEDDMRRWDERWQGENYA 240 Query: 241 YNLQATEQLKELASDKGISVAELALAWLLNQGENIVPIPGTRSVTRVEQNINAVNVNLTE 300 YNL A EQL++LA+ KGI+ A+LALAWLL QGE++VPIPGTRS R+E+N+ A++ LTE Sbjct: 241 YNLNAAEQLRKLAATKGITPAQLALAWLLAQGEDVVPIPGTRSAARLEENVGAIDAELTE 300 Query: 301 ADLGRIKEILPHGSAGSRYPAGMIENFTRD 330 ADL RI+E++P GSAGSRYPA M+ F D Sbjct: 301 ADLARIREVIPQGSAGSRYPASMMAGFRTD 330 >C1WFQ0.1 C1WFQ0_9ACTO Predicted oxidoreductase similar to aryl-alcohol dehydrogenase Length = 330 Score = 471 bits (1212), Expect = e-131, Method: Compositional matrix adjust. Identities = 232/330 (70%), Positives = 263/330 (79%), Gaps = 1/330 (0%) Query: 1 MQTRILGQQGLKVSSIGLGTMGMTMAYGPSNENEGIATIRRAYELGVNFFDTAELYGYGN 60 MQ R LG+QGL VS++GLGTMGMTMAYG ++ GIATIRRAYELG+ FFDTAELYG G Sbjct: 1 MQQRTLGRQGLHVSALGLGTMGMTMAYGAADAEGGIATIRRAYELGITFFDTAELYGSGT 60 Query: 61 GHNEQLLGKAVKDFRDKVVLATKFGFDMTAEQL-GTRFNSRPENIRKVAENSLRYLQTDY 119 G NEQLLG+AV FRD+VVLATKFGFDMT L G NSRPENIR+VAENSLRYL TD+ Sbjct: 61 GSNEQLLGRAVAGFRDEVVLATKFGFDMTKNPLEGEALNSRPENIRQVAENSLRYLGTDH 120 Query: 120 IDVFYQHIPDPDVPIEEVAGVVGDLIKEGKVKYFGLSNTGSKTIRRAHAVTPVSVLQTEY 179 +DVFYQH DP VPIEEVAGVVG+LI+ GKV+YFGLS G + IRRAHAV PVSVLQTEY Sbjct: 121 LDVFYQHRVDPAVPIEEVAGVVGELIQAGKVRYFGLSEAGPEAIRRAHAVQPVSVLQTEY 180 Query: 180 SIFEREIEDDILPTLNELGIGLVPYSPLGRGFLTDAVKPSHEYPEGDMRRFDERWQGENY 239 S+FERE+ED +LP + ELGIG VPYSPLGRGFLT V+P+HEYPE DMR FD+RWQG+NY Sbjct: 181 SLFEREVEDTVLPVVRELGIGFVPYSPLGRGFLTGDVRPAHEYPEDDMRSFDDRWQGKNY 240 Query: 240 IYNLQATEQLKELASDKGISVAELALAWLLNQGENIVPIPGTRSVTRVEQNINAVNVNLT 299 NL A +L A KGI+V +LALAWLL QG +IVPIPGTR V RVE+N AV V L Sbjct: 241 EANLAAVRELTAFAEGKGITVTQLALAWLLAQGNDIVPIPGTRKVHRVEENAGAVEVQLG 300 Query: 300 EADLGRIKEILPHGSAGSRYPAGMIENFTR 329 ADL RI+EILPHGS GSRY A + + R Sbjct: 301 AADLERIREILPHGSYGSRYLAQHMPEWAR 330 >Q82GC1.1 Q82GC1_STRAW Putative oxidoreductase Length = 340 Score = 439 bits (1129), Expect = e-121, Method: Compositional matrix adjust. Identities = 214/328 (65%), Positives = 256/328 (78%), Gaps = 1/328 (0%) Query: 1 MQTRILGQQGLKVSSIGLGTMGMTMAYGPSNENEGIATIRRAYELGVNFFDTAELYGYGN 60 + R LG QGL+ +IGLGTMGMTMAYG +E GIATI RAYELGV FDTAELYG G Sbjct: 13 LPKRTLGTQGLEAGAIGLGTMGMTMAYGAGDEPGGIATIHRAYELGVTLFDTAELYGMGT 72 Query: 61 GHNEQLLGKAVKDFRDKVVLATKFGFDMT-AEQLGTRFNSRPENIRKVAENSLRYLQTDY 119 G NEQLLG+AVK FRD+VVLATKFGFD++ +G +SRP +IR+V ENSLR+L TD+ Sbjct: 73 GSNEQLLGRAVKGFRDEVVLATKFGFDLSDPANVGVALDSRPGHIREVTENSLRHLGTDH 132 Query: 120 IDVFYQHIPDPDVPIEEVAGVVGDLIKEGKVKYFGLSNTGSKTIRRAHAVTPVSVLQTEY 179 IDV YQH DPDVPIE+VAG VG+LI EGKV+YFGLS G + IRRAHAV PVSVLQTEY Sbjct: 133 IDVLYQHRVDPDVPIEDVAGTVGELIAEGKVRYFGLSEAGPEIIRRAHAVHPVSVLQTEY 192 Query: 180 SIFEREIEDDILPTLNELGIGLVPYSPLGRGFLTDAVKPSHEYPEGDMRRFDERWQGENY 239 S+FER +E D+LP + ELGIG VPYSPLGRGFLT AV+P+ YP DMR +D+RWQ NY Sbjct: 193 SVFERAVEADVLPVVRELGIGFVPYSPLGRGFLTGAVRPASAYPADDMRSWDDRWQPGNY 252 Query: 240 IYNLQATEQLKELASDKGISVAELALAWLLNQGENIVPIPGTRSVTRVEQNINAVNVNLT 299 N+ A +L LA+ KGISV +LALAWLL QG++IVPIPGTRS R+ +N+ A +V LT Sbjct: 253 EKNVAAVSELTTLAAAKGISVTQLALAWLLAQGDDIVPIPGTRSPQRLAENVAAAHVTLT 312 Query: 300 EADLGRIKEILPHGSAGSRYPAGMIENF 327 ADL R++EILP G+AGSRYP M+ ++ Sbjct: 313 PADLVRVQEILPKGAAGSRYPEVMMPSW 340 >C2AA54.1 C2AA54_THECU Predicted oxidoreductase similar to aryl-alcohol dehydrogenase Length = 324 Score = 400 bits (1027), Expect = e-109, Method: Compositional matrix adjust. Identities = 203/330 (61%), Positives = 249/330 (75%), Gaps = 10/330 (3%) Query: 1 MQTRILGQQGLKVSSIGLGTMGMTMAYGPSNENEGIATIRRAYELGVNFFDTAELYGYGN 60 M+ R LG+QGL VS +G G MG+++AYGPS+E EG ATIRRA+ELGV FFDTAELYG Sbjct: 1 MRQRALGRQGLVVSELGYGAMGISIAYGPSDEKEGEATIRRAHELGVTFFDTAELYG--Q 58 Query: 61 GHNEQLLGKAVKDFRDKVVLATKFGFDMTAEQLGTR---FNSRPENIRKVAENSLRYLQT 117 G NE+++G A+ RD+VV+ATKFGFD T GTR +SRPE IR+V +NSLR+L T Sbjct: 59 GANERIVGAALAPVRDEVVIATKFGFDFT----GTRPSGLDSRPERIRQVVDNSLRHLGT 114 Query: 118 DYIDVFYQHIPDPDVPIEEVAGVVGDLIKEGKVKYFGLSNTGSKTIRRAHAVTPVSVLQT 177 D ID+ YQH DPDVPIEEVAG VG+LI+ GKV+YFGLS G TIRRAHAV PVS LQT Sbjct: 115 DVIDLLYQHRVDPDVPIEEVAGTVGELIEAGKVRYFGLSEAGENTIRRAHAVCPVSALQT 174 Query: 178 EYSIFEREIEDDILPTLNELGIGLVPYSPLGRGFLTDAVKPSHEYPEGDMRRFDERWQGE 237 EYS+FER++E + P L ELGIG V YSPLGRGFLT + KP EY D R +D R+Q Sbjct: 175 EYSLFERDVEA-LFPVLRELGIGFVAYSPLGRGFLTGSAKPGPEYDPSDFRHWDPRFQPG 233 Query: 238 NYIYNLQATEQLKELASDKGISVAELALAWLLNQGENIVPIPGTRSVTRVEQNINAVNVN 297 NY NL+AT L ELA+ KG +VA+LALAWLL +GE++VPIPGTRS R+E+N+ A ++ Sbjct: 234 NYEKNLEATRALAELAAAKGATVAQLALAWLLTRGEDVVPIPGTRSPKRLEENVAAADLV 293 Query: 298 LTEADLGRIKEILPHGSAGSRYPAGMIENF 327 L+ ADL RI EILP+G G+RYP GM+ + Sbjct: 294 LSRADLERIAEILPNGGFGARYPEGMLPTW 323 >Q0C022.1 Q0C022_HYPNA Oxidoreductase, aldo/keto reductase family Length = 323 Score = 396 bits (1018), Expect = e-108, Method: Compositional matrix adjust. Identities = 200/327 (61%), Positives = 243/327 (74%), Gaps = 5/327 (1%) Query: 1 MQTRILGQQGLKVSSIGLGTMGMTMAYGPSNENEGIATIRRAYELGVNFFDTAELYGYGN 60 M TR LG+ G VS+IG G MG YG ++ G+ IRRAY+ GVNFFDTAE+YG+G Sbjct: 1 MHTRKLGRNGPDVSAIGYGAMGFHAHYGAADPQAGLDLIRRAYDQGVNFFDTAEMYGWGE 60 Query: 61 GHNEQLLGKAVKDFRDKVVLATKFGFDMTAEQLGTRFNSRPENIRKVAENSLRYLQTDYI 120 NE+ +G+AVK FRD+VVLATKFGF GT NS+P +IRKV ENSLR+L D+I Sbjct: 61 --NEKFIGQAVKGFRDEVVLATKFGFTYEDGVYGT--NSQPGHIRKVTENSLRHLGVDHI 116 Query: 121 DVFYQHIPDPDVPIEEVAGVVGDLIKEGKVKYFGLSNTGSKTIRRAHAVTPVSVLQTEYS 180 D+ YQH DPDVPIE+VAG + DLI EGKVKYFGLS G +TIR+AHAV PV+VLQTEYS Sbjct: 117 DILYQHRVDPDVPIEDVAGTIRDLIAEGKVKYFGLSEAGPQTIRKAHAVQPVTVLQTEYS 176 Query: 181 IFEREIEDDILPTLNELGIGLVPYSPLGRGFLTDAVKPSHEYPEGDMRRFDERWQGENYI 240 +FER +E +LPTL ELGIG V YSPLGRGFLT AVKPS EY + DMRRFD RWQ N+ Sbjct: 177 VFERNVET-VLPTLRELGIGFVAYSPLGRGFLTSAVKPSAEYEDTDMRRFDPRWQPGNFE 235 Query: 241 YNLQATEQLKELASDKGISVAELALAWLLNQGENIVPIPGTRSVTRVEQNINAVNVNLTE 300 NL A QL ELA DKGI+V++LALAWLL QGE+IVPIPGTR + R+E+N+ + V L+ Sbjct: 236 KNLDAVRQLGELAKDKGITVSQLALAWLLAQGEDIVPIPGTRRIDRLEENLASAEVTLST 295 Query: 301 ADLGRIKEILPHGSAGSRYPAGMIENF 327 DL I+ ILP G+ G+RY M+ ++ Sbjct: 296 DDLAGIRNILPEGAFGARYAGDMVPDW 322 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.137 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,042,445,770 Number of extensions: 89421994 Number of successful extensions: 263719 Number of sequences better than 1.0e-05: 6839 Number of HSP's gapped: 246577 Number of HSP's successfully gapped: 6873 Length of query: 330 Length of database: 3,060,696,203 Length adjustment: 136 Effective length of query: 194 Effective length of database: 1,779,438,163 Effective search space: 345211003622 Effective search space used: 345211003622 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)