BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0045.p (387 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A8FC56.1 A8FC56_BACP2 MFS family major facilitator transporter 488 e-136 B4AF33.1 B4AF33_BACPU MFS family major facilitator transporter 486 e-135 Q5WAR7.1 Q5WAR7_BACSK Putative uncharacterized protein 323 1e-86 A3I8L7.1 A3I8L7_9BACI Putative uncharacterized protein 269 3e-70 A9VI24.1 A9VI24_BACWK Major facilitator superfamily MFS_1 251 1e-64 >A8FC56.1 A8FC56_BACP2 MFS family major facilitator transporter Length = 384 Score = 488 bits (1257), Expect = e-136, Method: Compositional matrix adjust. Identities = 255/385 (66%), Positives = 304/385 (78%), Gaps = 1/385 (0%) Query: 1 MKKLFSLFALCIAAMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVA 60 MKK+F L AL +AA+NLRP+ITSVAS++ IQS LG+NALTASLLTTLPVLCMG+F+PVA Sbjct: 1 MKKIFYLTALFLAALNLRPIITSVASMMSMIQSELGVNALTASLLTTLPVLCMGVFAPVA 60 Query: 61 TVLSHRIGLERTIFFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKK 120 T LS R GLERT+FFS+ LIT+AT LRG+ SV +LL+TA GG+GIS AGP+LSSFIKK Sbjct: 61 TTLSRRFGLERTLFFSILLITIATALRGTSQSVAILLMTAFAGGVGISFAGPLLSSFIKK 120 Query: 121 YFPEKPDIVSIYSISMTIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFV 180 YFP P IVS+YSISMTIGAALASGFTIP+Y S ++LP ALSCWAILG IAL+ W+ Sbjct: 121 YFPRNPGIVSVYSISMTIGAALASGFTIPIYLRS-HHLPFALSCWAILGVIALILWVGIA 179 Query: 181 RNKHHQTNATIRQRLPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMF 240 R T+ +LP+ NKKAIQFT+FFG M+SMFYS+T+WISPIALSFG S + +AM Sbjct: 180 RKNEQSDLVTLPLKLPLRNKKAIQFTLFFGFMSSMFYSLTAWISPIALSFGNSPQYAAML 239 Query: 241 LTIFTLIQIPIAFLVPGSVKRSGKPKXXXXXXXXXXXIGVGLLLFQFPMLLAVLFLGIGA 300 LTIFTLIQIP+A LVP V R GKPK IG+ LLL P+L AV+FLG+GA Sbjct: 240 LTIFTLIQIPVALLVPAVVTRLGKPKLFLILCSLSELIGLILLLLPMPILPAVIFLGMGA 299 Query: 301 GGLFPLALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNSSIFAM 360 GGLFPLALMLPI ET T EEAGTWSAMSQMGGY+MG FGP +IG + D SG+F +SI AM Sbjct: 300 GGLFPLALMLPIIETDTPEEAGTWSAMSQMGGYIMGGFGPFIIGLVFDLSGHFYASIVAM 359 Query: 361 LIIIMVMIGVQVSMTMGKKDTEVQE 385 L+I+M+MI VQ+SM +G+K+ EV Sbjct: 360 LVIVMLMISVQLSMKLGRKEEEVSS 384 >B4AF33.1 B4AF33_BACPU MFS family major facilitator transporter Length = 384 Score = 486 bits (1250), Expect = e-135, Method: Compositional matrix adjust. Identities = 254/380 (66%), Positives = 303/380 (79%), Gaps = 1/380 (0%) Query: 1 MKKLFSLFALCIAAMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVA 60 MKK+F L AL +AA+NLRP+ITSVAS++ IQS LG+NALTASLLTTLPVLCMG+F+PVA Sbjct: 1 MKKIFYLAALFLAALNLRPIITSVASMMSMIQSELGVNALTASLLTTLPVLCMGVFAPVA 60 Query: 61 TVLSHRIGLERTIFFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKK 120 T LS R GLERT+FFS+ LIT+AT LRG+ SV +LL+TA GG+GIS AGP+LSSFIKK Sbjct: 61 TTLSRRFGLERTLFFSILLITIATALRGTSQSVAILLMTAFAGGVGISFAGPLLSSFIKK 120 Query: 121 YFPEKPDIVSIYSISMTIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFV 180 YFP+ P IVS+YSISMTIGAALASGFTIP+Y S ++LP ALSCWAILG IAL+ W+ Sbjct: 121 YFPKNPGIVSVYSISMTIGAALASGFTIPIYLMS-HHLPFALSCWAILGVIALILWVGIA 179 Query: 181 RNKHHQTNATIRQRLPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMF 240 R + T+ +LP+ NKKAIQFT+FFG M+SMFYS+T+WISPIALSFG S + +AM Sbjct: 180 RKNEQSDHVTLPLKLPLRNKKAIQFTLFFGFMSSMFYSLTAWISPIALSFGNSPQYAAML 239 Query: 241 LTIFTLIQIPIAFLVPGSVKRSGKPKXXXXXXXXXXXIGVGLLLFQFPMLLAVLFLGIGA 300 LTIFTLIQIP+A LVP V R GKPK IG+ LL P+L AV+FLG+GA Sbjct: 240 LTIFTLIQIPVALLVPAVVTRLGKPKLFLILCSLSELIGLIFLLLPMPILPAVIFLGMGA 299 Query: 301 GGLFPLALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNSSIFAM 360 GGLFPLALMLPI ET+T EEAGTWSAMSQMGGY+MG FGP IG + D SG+F +SI AM Sbjct: 300 GGLFPLALMLPIIETRTPEEAGTWSAMSQMGGYIMGGFGPFFIGLVFDLSGHFYASIVAM 359 Query: 361 LIIIMVMIGVQVSMTMGKKD 380 L+I+M+MIGVQ+SM +GKK Sbjct: 360 LVIVMLMIGVQLSMNLGKKR 379 >Q5WAR7.1 Q5WAR7_BACSK Putative uncharacterized protein Length = 381 Score = 323 bits (829), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 182/353 (51%), Positives = 245/353 (69%), Gaps = 1/353 (0%) Query: 1 MKKLFSLFALCIAAMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVA 60 M+ ++ + AL +A++N+RP ITSVA LL TIQ LG++ L +SLLTTLPVLCMGIF+P A Sbjct: 1 MRTIYFILALILASLNMRPAITSVAPLLDTIQQQLGMSGLASSLLTTLPVLCMGIFAPFA 60 Query: 61 TVLSHRIGLERTIFFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKK 120 +S +IGLE+ IF SL L+T AT +RG SV++L+VTA + GIGI +AGP+LS FIK+ Sbjct: 61 AKISQKIGLEKAIFLSLLLLTAATAVRGITGSVIVLIVTAFLAGIGIGIAGPLLSGFIKQ 120 Query: 121 YFPEKPDIVSIYSISMTIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFV 180 YFP +P VSIYS+SM++GA +A+G ++P+Y + N AL+ WA+LG AL WI Sbjct: 121 YFPTRPGFVSIYSVSMSLGAGIAAGLSVPIYNSAGQNWGFALASWAVLGVAALTFWIKLA 180 Query: 181 RNKHHQTNATIRQRLPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMF 240 K + A + RLP+ NK+AI T+FF LM+SMFYS+T+WI+PI GYS+ ++A Sbjct: 181 LQKRTEVTAA-KARLPLKNKRAILVTLFFALMSSMFYSVTAWIAPIVQDMGYSKSTAAAM 239 Query: 241 LTIFTLIQIPIAFLVPGSVKRSGKPKXXXXXXXXXXXIGVGLLLFQFPMLLAVLFLGIGA 300 LT+FT IQIP + L+P V + G+ G+G+LL L A L LGIGA Sbjct: 240 LTVFTFIQIPTSMLLPTLVAKFGRLTTVLVACSMLELAGIGILLAGLHPLPAALLLGIGA 299 Query: 301 GGLFPLALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNF 353 GGLFPLALMLPI ET + +EA WS+M+Q GGY++ A GPL +G ISD G+F Sbjct: 300 GGLFPLALMLPIVETDSPQEASAWSSMNQGGGYILAAIGPLAVGAISDMFGSF 352 >A3I8L7.1 A3I8L7_9BACI Putative uncharacterized protein Length = 390 Score = 269 bits (688), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 160/357 (44%), Positives = 220/357 (61%), Gaps = 14/357 (3%) Query: 9 ALCIAAMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHRIG 68 A+ +A++NLRP+ITSV+ LLGTI+ L ++A +ASLLT+L VLCMG+F+P+A L++R Sbjct: 12 AIILASLNLRPVITSVSPLLGTIRESLHMSAASASLLTSLAVLCMGLFAPIALKLANRWS 71 Query: 69 LERTIFFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEKPDI 128 +ER I +SL LI LAT R S ++ VTAL GIGI++ GP+LS FIK F I Sbjct: 72 IERAIAYSLILIGLATTSRYFAYSAWVMHVTALFAGIGIAIVGPLLSGFIKAKFANPSRI 131 Query: 129 VSIYSISMTIGAALASGFTIPVYT--HSEYNLPIALSCWAILGFIALLAWIAFVRNKHHQ 186 V IYS+++ +GAAL SG +IP+ T HS I+ + WA+L IAL W + K H Sbjct: 132 VGIYSLALVVGAALGSGLSIPLSTIFHS---WQISAASWALLAIIALFFWWKPIERKTHS 188 Query: 187 TNAT-------IRQRLPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAM 239 T+ T I+Q L ++KA T FFGLMA MFY+I +W+ PIA GYS+ + M Sbjct: 189 TSTTGNKNTISIKQIL--TSEKAWLLTGFFGLMAFMFYTIMAWLPPIAEQMGYSKHDAGM 246 Query: 240 FLTIFTLIQIPIAFLVPGSVKRSGKPKXXXXXXXXXXXIGVGLLLFQFPMLLAVLFLGIG 299 LT+ T+ Q+P FLVP RS IG+ +LLF L+ + +GIG Sbjct: 247 MLTLLTIAQMPATFLVPILNNRSQHRAVWLVACSLLELIGLSMLLFSINPWLSSVLIGIG 306 Query: 300 AGGLFPLALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNSS 356 AGGLF L L LPI E + +EA +AM+Q G+V A GPL +G + D +GNF ++ Sbjct: 307 AGGLFSLGLTLPIDEAEDIQEASFLAAMTQSVGFVFAALGPLFVGLVRDYTGNFTAA 363 >A9VI24.1 A9VI24_BACWK Major facilitator superfamily MFS_1 Length = 396 Score = 251 bits (640), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 147/380 (38%), Positives = 222/380 (58%), Gaps = 5/380 (1%) Query: 7 LFALCIAAMNLRPMITSVASLLGTIQSHLGINALTASLLTTLPVLCMGIFSPVATVLSHR 66 + AL ++NLR ITSV+ LL TI+ L I+ + S LT +PV CMG F+ + + + Sbjct: 13 ILALFFVSINLRIGITSVSPLLETIRQDLNISNFSVSFLTAIPVFCMGTFALLTGKVIKK 72 Query: 67 IGLERTIFFSLFLITLATVLRGSDSSVMLLLVTALVGGIGISLAGPMLSSFIKKYFPEKP 126 G ER I L LI AT +R SS+ L ++L GIGI+LAGP+LS FIK+ FP K Sbjct: 73 YGAERAIMTCLILIGFATCMRAFTSSISTLFASSLFIGIGIALAGPLLSGFIKEKFPTKI 132 Query: 127 DI-VSIYSISMTIGAALASGFTIPVYTHSEYNLPIALSCWAILGFIALLAWIAFV-RNKH 184 + + IYS+ M GA+L++G TIP+ + + +AL+ W +L IA++ W + R K+ Sbjct: 133 GLMIGIYSVGMGTGASLSAGLTIPLQHVLKDDWNMALAFWGVLTIIAIIFWYPVMKRKKN 192 Query: 185 HQTNATIRQRLPIANKKAIQFTIFFGLMASMFYSITSWISPIALSFGYSRESSAMFLTIF 244 T LP+ NKKA FTIFFGL + +FYSIT+W++P + G S E + +T+F Sbjct: 193 TSTQNKKNNSLPLRNKKAWLFTIFFGLQSGIFYSITTWLAPANQNMGVSSEQAGTLITVF 252 Query: 245 TLIQIPIAFLVPGSVKRSGKPKXXXXXXXXXXXIGVGLLLFQF--PMLLAVLFLGIGAGG 302 T++Q+ +FL+P +G+ L+++ P + ++L LGIG GG Sbjct: 253 TVVQMICSFLIPTLADIYKNRALWLLGSICFVLVGLSLMIYPLTTPWIPSIL-LGIGLGG 311 Query: 303 LFPLALMLPISETKTAEEAGTWSAMSQMGGYVMGAFGPLLIGWISDQSGNFNSSIFAMLI 362 +FPLALMLP+ ETKT+E+A W+AM Q GGY+MG F P+L G D ++ M + Sbjct: 312 VFPLALMLPLYETKTSEDASAWTAMMQSGGYIMGGFIPVLAGIARDYFNSYTQVFIIMAL 371 Query: 363 IIMVMIGVQVSMTMGKKDTE 382 + ++ + + M K++ E Sbjct: 372 LSFILFLLTLVMNKRKRNAE 391 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.328 0.140 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,013,894,262 Number of extensions: 78308875 Number of successful extensions: 383156 Number of sequences better than 1.0e-05: 833 Number of HSP's gapped: 381709 Number of HSP's successfully gapped: 840 Length of query: 387 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 249 Effective length of database: 1,760,596,133 Effective search space: 438388437117 Effective search space used: 438388437117 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)