BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0031.p (570 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A2ICH1.1 A2ICH1_THESJ Xylosidase 412 e-113 A9BJ32.1 A9BJ32_PETMO Putative outer membrane protein 411 e-112 B7AMQ6.1 B7AMQ6_9BACE Putative uncharacterized protein 405 e-111 C5EM91.1 C5EM91_9FIRM Xylosidase 400 e-109 A7VQ33.1 A7VQ33_9CLOT Putative uncharacterized protein 394 e-107 >A2ICH1.1 A2ICH1_THESJ Xylosidase Length = 638 Score = 412 bits (1059), Expect = e-113, Method: Compositional matrix adjust. Identities = 231/561 (41%), Positives = 311/561 (55%), Gaps = 62/561 (11%) Query: 1 VYLDGKSLYESDSANKLHNPQI-------WPQA----KCAEDSLMQWYAEASSTTTTIWA 49 VYL+G S YE + L NPQ+ W Q K AE + WYAE TTI+A Sbjct: 126 VYLNGMSFYEVTNYEDLFNPQLRTEVLDHWTQKIVPIKNAEQTKYVWYAEVDREKTTIYA 185 Query: 50 NFGGKDPRQENVEINVRPYCFWPEKTGRNYITVRGFTLQQASPQWAPPTALQEGLIGPHW 109 NF G DP +E VEINVR CF+P +TG +YITV+GF + A+ WAPPTA Q GLIGP+W Sbjct: 186 NFQGADPNEEFVEINVRRSCFYPVETGIDYITVKGFEMAHAATPWAPPTADQPGLIGPNW 245 Query: 110 SKGWIIENNVINESKCTGISLGKDITAGPNEYSNNRSKGGTQREQEVIFRALRKDWHKDH 169 SKGWIIE+N+I+++KC+ IS+GK+ T G N S + K G Q + E +F A R W K+ Sbjct: 246 SKGWIIEDNIIHDAKCSAISIGKEATTGNNYRSIRKDKPGYQYQLEAVFNAKRNGWSKEK 305 Query: 170 IGSHIVRNNVIHDCEQAGIVGHLGAAFSRIYQNRIYNIHHKRIFHGAEVGGIKLHAALDT 229 IGSHI+RNN I+DC Q IVGHLG FS IY N IYNI KR F+G E+ GIKLHAA+D Sbjct: 306 IGSHIIRNNTIYDCGQNAIVGHLGGVFSEIYNNHIYNIALKREFYGHEIAGIKLHAAIDV 365 Query: 230 QICGNVIYSSYRALWLDWQAQGTRISRNIFYDNLSEDLFVEVCHGPYMVDHNLFLSPMNF 289 QI N I+ LWLDW+AQGTR+S+N+FY+N + D+FVEV HGPY+VDHN+ S Sbjct: 366 QIHHNRIHDCSLGLWLDWEAQGTRVSKNLFYNN-NRDVFVEVSHGPYLVDHNILSSEYAI 424 Query: 290 RNMAQGGAFVHNLFTGRFVVRSELTRYTPYHMPHETAVAGYSNITGGDDRYYNNIFIGDD 349 NM+QGGA+++NL G+ R L R T YH+PH T VAG++ + GGDDR+YNNIFIG + Sbjct: 425 DNMSQGGAYINNLIAGKMNQRKVLNRSTQYHLPHSTEVAGFAFVYGGDDRFYNNIFIGKE 484 Query: 350 DPGKEPLPMTFFEHLPLTPREDMDYGDAAVMDGVPDNSRCYLHPAGLGGYNDYPSAHQKQ 409 G E + + + + + E ++ + P LG + Sbjct: 485 --GLENVGTSHYNNCTTSLEEYIEKVNEV--------------PGDLGEF---------- 518 Query: 410 WWEYTKEEIAALKESGKQWQPDKMALPVTIQGNLYLKAAVPSAHESNAKVYVNNGIEIEI 469 +++ PV I N Y A P E + V + ++ I Sbjct: 519 ---------------------ERVEQPVYINKNAYFNGAEPFEKEKDNLVKKDFDPKLAI 557 Query: 470 DPATCRVQVHVHSPEIIRGAAPTVVTTNLLGRSYHAEMHYEKPDGSPYRFDHDFFHKLRP 529 V + + P+ + +T L R + YE PDG D D+ +P Sbjct: 558 IDEGDEVYLSLQLPDEFENIVGDIHSTKTLERVRIVDAEYESPDGKELVLDTDYLDAKKP 617 Query: 530 EKNVTPGPFEIF--GNSLVKM 548 E N + GP + GN+ +K+ Sbjct: 618 E-NSSIGPIALLKKGNNYIKV 637 >A9BJ32.1 A9BJ32_PETMO Putative outer membrane protein Length = 638 Score = 411 bits (1056), Expect = e-112, Method: Compositional matrix adjust. Identities = 235/552 (42%), Positives = 304/552 (55%), Gaps = 66/552 (11%) Query: 1 VYLDGKSLYESDSANKLHNPQI-------WPQAKCA----EDSLMQWYAEASSTTTTIWA 49 VYL+G S YE++S L +P+I W Q K E + WYAE TTI+A Sbjct: 126 VYLNGMSFYEANSFEDLKDPKIRTETVDHWTQKKVPVLNPEQTKYVWYAEVDDQKTTIYA 185 Query: 50 NFGGKDPRQENVEINVRPYCFWPEKTGRNYITVRGFTLQQASPQWAPPTALQEGLIGPHW 109 NF G +P +E VEINVR CF+P++ GR+YITV+GF + A+ W PPTA Q GLIG +W Sbjct: 186 NFHGANPNEELVEINVRRSCFYPDQIGRDYITVKGFEMAHAATPWVPPTADQPGLIGANW 245 Query: 110 SKGWIIENNVINESKCTGISLGKDITAGPNEYSNNRSKGGTQREQEVIFRALRKDWHKDH 169 SKGWIIENN+I++SKC+ IS+GK+ + G +S + K G Q + E +F A W K+ Sbjct: 246 SKGWIIENNIIHDSKCSAISIGKEGSTGHLYHSTRKDKTGHQYQLESVFSAEHIGWSKES 305 Query: 170 IGSHIVRNNVIHDCEQAGIVGHLGAAFSRIYQNRIYNIHHKRIFHGAEVGGIKLHAALDT 229 IGSHI+RNN I+DC Q IVGHLG FS IY N IY I KR F G E+ GIKLHAA+D Sbjct: 306 IGSHIIRNNTIYDCGQNAIVGHLGCVFSEIYNNHIYRIAIKREFWGHEIAGIKLHAAIDV 365 Query: 230 QICGNVIYSSYRALWLDWQAQGTRISRNIFYDNLSEDLFVEVCHGPYMVDHNLFLSPMNF 289 QI N I+ LWLDWQAQGTR+S+N+FY N + DLFVEV HGPY++DHN+ S Sbjct: 366 QIYHNRIHDCSLGLWLDWQAQGTRVSKNLFYKN-NRDLFVEVSHGPYVIDHNILASEYAI 424 Query: 290 RNMAQGGAFVHNLFTGRFVVRSELTRYTPYHMPHETAVAGYSNITGGDDRYYNNIFIGDD 349 NM+QGGA+++NL G+ R L RYTPYH PH T VAGY+ + GGDDR+YNNIFIG D Sbjct: 425 DNMSQGGAYINNLIAGKMEKRKVLNRYTPYHRPHSTTVAGYACVYGGDDRFYNNIFIGKD 484 Query: 350 DPGKEPLPMTFFEHLPLTPREDMDYGDAAVMDGVPDNSRCYLHPAGLGGYNDYPSAHQKQ 409 ++GV G Y ++ ++ + Sbjct: 485 G-----------------------------IEGV-----------GTSHYMNHTTSLE-- 502 Query: 410 WWEYTKEEIAALKESGKQWQPDKMAL---PVTIQGNLYLKAAVPSAHESNAKVYVNNGIE 466 EY K K K W + L PV I N Y A P E V + + Sbjct: 503 --EYMK------KVDEKGWDIKTLELIEQPVYINNNAYFNGAEPFEREKEKLVDKSFDSK 554 Query: 467 IEIDPATCRVQVHVHSPEIIRGAAPTVVTTNLLGRSYHAEMHYEKPDGSPYRFDHDFFHK 526 + V + P+ A V +TN L R + +E+PDG D DF K Sbjct: 555 FSVIEKGEEVYMFCELPDTFEDIAGKVCSTNSLERVRIVDADFERPDGGNLVLDTDFLDK 614 Query: 527 LRPEKNVTPGPF 538 +PEK+ + GP Sbjct: 615 QKPEKS-SLGPI 625 >B7AMQ6.1 B7AMQ6_9BACE Putative uncharacterized protein Length = 676 Score = 405 bits (1041), Expect = e-111, Method: Compositional matrix adjust. Identities = 220/539 (40%), Positives = 306/539 (56%), Gaps = 45/539 (8%) Query: 1 VYLDGKSLYESDSANKLHNPQIWPQAKCAEDSLMQWYAEASSTTTTIWANFGGKDPRQEN 60 V+L+GKS YE DS +K+ NPQ +P +K SL QWYAE T T IWANF +P +E Sbjct: 165 VFLNGKSFYEVDSLSKVRNPQEFPDSKNKAQSLYQWYAEVGDTETVIWANFHKYNPNKEL 224 Query: 61 VEINVRPYCFWPEKTGRNYITVRGFTLQQASPQWAPPTALQEGLIGPHWSKGWIIENNVI 120 VEIN RP CF+P++ G NYITV+GF + QA+ QWAPPT +Q+GL+GP+WSKGWIIE N I Sbjct: 225 VEINARPACFFPKEQGVNYITVQGFKMSQAAVQWAPPTGVQKGLLGPNWSKGWIIEGNEI 284 Query: 121 NESKCTGISLGKDITAGPNEYSNNRSKGGTQREQEVIFRALRKDWHKDHIGSHIVRNNVI 180 + +KC+GISLGKD +G N ++ +S G RE E + +A R W+K++IGSH+VRNNVI Sbjct: 285 SNAKCSGISLGKDFASGNNLWTLEQSIVGFNREIESVIKAWRMGWNKENIGSHVVRNNVI 344 Query: 181 HDCEQAGIVGHLGAAFSRIYQNRIYNIHHKRIFHGAEVGGIKLHAALDTQICGNVIYSSY 240 DCEQ GI GH+GA FS I N IY+IH KR F GAE+G IKLHA +D + N + + Y Sbjct: 345 FDCEQTGICGHMGAIFSMIENNHIYDIHTKRQFTGAEIGCIKLHAPIDVVVKDNWLDNGY 404 Query: 241 RALWLDWQAQGTRISRNIFYDNLSEDLFVEVCHGPYMVDHNLFLSPMNFRNMAQGGAFVH 300 R +WLDWQ GTRI+ N+F +N D+ +EV HGP ++D+N+ LS + N++QG A+VH Sbjct: 405 RGIWLDWQCIGTRITGNLFTNNDFYDIMIEVTHGPCLIDNNILLSANSVNNLSQGTAWVH 464 Query: 301 NLFTGRFVVRSELTRYTPYHMPHETAVAGYSNITGGDDRYYNNIFIGDDDPGKEPLPMTF 360 NL G VR RYTPYH PH T + G ++I GD+R YNNI +P + Sbjct: 465 NLIGGVVTVRPIPIRYTPYHEPHSTDIIGLTSILCGDERLYNNILT--------VVPGSQ 516 Query: 361 FEHLPLTPREDMDYGDAAVMDGVPDNSRCYLHPAGLGGYNDYPSAHQKQWWEYTKEEIAA 420 EH +D G Y+ +P + + E + + Sbjct: 517 SEH-------KLD---------------------GFSPYDSHPEYKEGIYQEMNRTSRGS 548 Query: 421 LKESGKQWQPDKMALPVTIQGNLYLKAAVPSAHESNAKVYVNNGIEIEIDPATCRVQVHV 480 + Q L + NLY P AHE + N+ + I+ V + + Sbjct: 549 V-------QSKDFTLAMYTGANLYYDGVKPYAHEP-YNIVSNHALAPTIEQRGDEVVLKL 600 Query: 481 HSPEIIRGAAPTVVTTNLLGRSYHAEMHYEKPDGSPYRFDHDFFHKLRPEKNVTPGPFE 539 + I ++ T +G ++ + ++E PDG+P + D+ R + GPFE Sbjct: 601 NFDNNISQLQTQIIDTEKMGVTFFSNGYFENPDGTPITLNTDYLGVSRGDA-PKAGPFE 658 >C5EM91.1 C5EM91_9FIRM Xylosidase Length = 665 Score = 400 bits (1027), Expect = e-109, Method: Compositional matrix adjust. Identities = 235/547 (42%), Positives = 309/547 (56%), Gaps = 30/547 (5%) Query: 1 VYLDGKSLYESDSANKLHNPQIWPQAKCAEDSLM-QWYAEASSTTTTIWANFGGKDPRQE 59 VY +G++LYE D +KL N + A E S +WY E T I+A F P Sbjct: 126 VYFNGQALYEEDCLDKLWNDEPLEWAWDREYSRKHKWYTEQDGDVTVIYARFPDGAPHGN 185 Query: 60 NVEINVRPYCFWPEKTGRNYITVRGFTLQQASPQWAPPTALQEGLIGPHWSKGWIIENNV 119 VEINVRP F+PE TG +YITV GFTL+QA+ QWAPPTA Q+GLIGPHWS GWIIE+ V Sbjct: 186 LVEINVRPRVFFPEHTGMDYITVSGFTLRQAATQWAPPTAFQDGLIGPHWSLGWIIEDCV 245 Query: 120 INESKCTGISLGKDITAGPNEYSNNRSKGGTQREQEVIFRALRKDWHKDHIGSHIVRNNV 179 I++S+C+GISLGK N ++ R K GTQ E++++ A W ++ +GSHIVR N Sbjct: 246 ISDSRCSGISLGKCRHPSDNRWTKERVKSGTQLERDLVHYAQHTGWSRETVGSHIVRRNR 305 Query: 180 IHDCEQAGIVGHLGAAFSRIYQNRIYNIHHKRIFHGAEVGGIKLHAALDTQICGNVIYSS 239 I +C Q GIVGHLG FS I N I++I+ F G+E+GGIKLH A+DT I N I+ Sbjct: 306 IFNCGQTGIVGHLGGIFSLIEGNEIHHINTYGEFSGSELGGIKLHGAIDTVIRRNHIHHC 365 Query: 240 YRALWLDWQAQGTRISRNIFYDN-------LSEDLFVEVCHGPYMVDHNLFLSPMNFRNM 292 +R +WLDWQ QGTR+S N+F+DN SEDL++EV HGP +VD+NLFLS R Sbjct: 366 HRGIWLDWQTQGTRVSCNLFHDNHGEEDTRTSEDLYIEVSHGPALVDNNLFLSRFALRER 425 Query: 293 AQGGAFVHNLFTGRFVVRSELTRYTPYHMPHETAVAGYSNITGGDDRYYNNIFIGDDDPG 352 +QG AFVHN+ G + S R+TPYH PHET + G I GDDR+YNNIFI Sbjct: 426 SQGLAFVHNIIAGPIDIGSSEGRFTPYHFPHETDIMGCMTINNGDDRFYNNIFI------ 479 Query: 353 KEPLPMTFFEHLPLTPREDMDYGDAAVMDGVPDNSRCYLHPAGLGGYNDYPSAHQKQWWE 412 P P+E + V V N + + AGL Y+DYP H + + + Sbjct: 480 -----------QPDLPQEKRQVRNPEVARLVGMNPQVNIE-AGLHIYDDYP-LHDEYYHQ 526 Query: 413 YTKEEIAALKESGKQWQPDKMALPVTIQGNLYLKAAVPSAHESNAKVYVNNGIEIEIDPA 472 +T E G+++ LPV I GN Y A P E V N + + Sbjct: 527 FTDEGTPDTS-WGRRYDYTSH-LPVYISGNAYFNGARPYVKEQLNHVDTENRVYFNLIEK 584 Query: 473 TCRVQVHVHSPEIIRGAAPTVVTTNLLGRSYHAEMHYEKPDGSPYRFDHDFFHKLRPEKN 532 + + + ++T++LG S+ E YE PDGSP R D DF + R + + Sbjct: 585 EGSYYFSTNLYDYLPRMETEFISTSMLGMSFETEQPYENPDGSPVRIDEDFLGRKRGQ-H 643 Query: 533 VTPGPFE 539 TPGPFE Sbjct: 644 PTPGPFE 650 >A7VQ33.1 A7VQ33_9CLOT Putative uncharacterized protein Length = 669 Score = 394 bits (1013), Expect = e-107, Method: Compositional matrix adjust. Identities = 231/568 (40%), Positives = 325/568 (57%), Gaps = 62/568 (10%) Query: 1 VYLDGKSLYESDSANKLHNPQIWPQAKCAEDSLMQWYAEASSTTTTIWANFGGKDPRQEN 60 VYL+GK++YE+ S ++ NP ++ ++ + ++ QWY T I+ANF G DP QE Sbjct: 125 VYLNGKAMYEAVSLERVINPVVYQESWDPDFTVYQWYTRQEGGYTEIYANFQGADPNQET 184 Query: 61 VEINVRPYCFWPEKTGRNYITVRGFTLQQASPQWAPPTALQEGLIGPHWSKGWIIENNVI 120 VEINVR CF+P+KTG NYIT+ GFT++QA+ WAPPTA QEG+IGPHWSKGWIIE+ + Sbjct: 185 VEINVRRNCFYPDKTGVNYITLSGFTVKQAATTWAPPTAYQEGMIGPHWSKGWIIEDCEV 244 Query: 121 NESKCTGISLGKDITA-GPNEYSNNRSKGGTQREQEVIFRALRKDWHKDHIGSHIVRNNV 179 ++S+C+GISLGK + N++S K GTQ E++ I +A + W K+ IGSHIVR Sbjct: 245 SDSRCSGISLGKYLQPNNENKWSTKFLKDGTQTERDAICQAQVEGWSKETIGSHIVRRCN 304 Query: 180 IHDCEQAGIVGHLGAAFSRIYQNRIYNIHHKRIFHGAEVGGIKLHAALDTQICGNVIYSS 239 IHDC Q GIVGHLG FS I N I++I++K+ GAE+GGIK+HAA+D I N + Sbjct: 305 IHDCGQTGIVGHLGGVFSVIEDNHIHHINNKQDLAGAEIGGIKMHAAIDVMIRRNHFHHC 364 Query: 240 YRALWLDWQAQGTRISRNIFYDN-------------LSEDLFVEVCHGPYMVDHNLFLSP 286 R LWLDWQAQGTR+++N+F+DN L ED+FVEV HGP ++D+NL LS Sbjct: 365 TRGLWLDWQAQGTRVTQNLFHDNVPPQGTKITNSLALGEDIFVEVSHGPTLIDNNLLLSS 424 Query: 287 MNFRNMAQGGAFVHNLFTGRFV----------VRSELTRYTPYHMPHETAVAGYSNITGG 336 R QG A VHNL G F R RYTPYH+PH T VAG+ I G Sbjct: 425 CAGRLSTQGLALVHNLIAGSFTWVGAGTDNNGKRFPTPRYTPYHIPHRTEVAGFMTILHG 484 Query: 337 DDRYYNNIFIGDDDPGKEPLPMTFFEHLPLTPREDMD-YGDA---AVMDGVPDNSRCYLH 392 D R+YNNIF+ + R+D+ Y ++ + +DG+ C Sbjct: 485 DARFYNNIFVQQE------------------VRKDLTAYSESIGKSTLDGI--QFLCGTK 524 Query: 393 PAGLGGYNDYPSAHQKQWWEYTKEEIAALKESGKQWQPDKMALPVTIQGNLYLKAAVPSA 452 P Y+ YP+A + +++ A ++ G + LPV GN+Y A P Sbjct: 525 P-----YDGYPTAEEY----FSRFGYGAAEDRGNR-DIYYDHLPVYTGGNVYFNGAQPCD 574 Query: 453 HESNAKVYVNNGIEIEI--DPATCRVQVHVHSPEIIRGAAPTVVTTNLLGRSYHAEMHYE 510 E N ++ + I +++ D +Q +++ E + +V+T LLG ++ E +E Sbjct: 575 AEENYQMDSQHPIFLKLGEDGEGWYLQTNLY--EYLPRFETQMVSTELLGEAFEPEQKFE 632 Query: 511 KPDGSPYRFDHDFFHKLRPEKNVTPGPF 538 PDG+P F+ D+F R GPF Sbjct: 633 SPDGTPIVFNQDYFGARREPVMPLAGPF 660 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,914,883,797 Number of extensions: 180102151 Number of successful extensions: 313628 Number of sequences better than 1.0e-05: 28 Number of HSP's gapped: 313554 Number of HSP's successfully gapped: 28 Length of query: 570 Length of database: 3,060,696,203 Length adjustment: 141 Effective length of query: 429 Effective length of database: 1,732,333,088 Effective search space: 743170894752 Effective search space used: 743170894752 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)