BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0029.p (189 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q032L1.1 Q032L1_LACLS Transcriptional regulator 107 6e-22 A2RHW6.1 A2RHW6_LACLM Transcriptional regulator, TetR family 107 7e-22 Q9CIW9.1 Q9CIW9_LACLA Transcriptional regulator 104 4e-21 Q5YRI3.1 Q5YRI3_NOCFA Putative transcriptional regulator 97 8e-19 Q2IK84.1 Q2IK84_ANADE Putative transcriptional regulator, TetR f... 92 2e-17 >Q032L1.1 Q032L1_LACLS Transcriptional regulator Length = 181 Score = 107 bits (266), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 62/175 (35%), Positives = 103/175 (58%), Gaps = 9/175 (5%) Query: 2 IKLKSKEKLQAAATKLFMEKGYENTTVQEIASLAGVTERTFFRQFKDKSDVLFDTNNELG 61 +K S+EKL AA +L +GY+NTTV +IA+ AG+TERTFFR+FK+K+D+ F G Sbjct: 1 MKKSSQEKLNEAALELITAQGYDNTTVLQIATKAGLTERTFFRKFKNKADIFFAG----G 56 Query: 62 QRVAAFITDNV--DVEKKPLKLAVEGFVSTSXXXXXXXGSRERVLARNQIIQSNPDLQER 119 ++ + + + + E PL + ++G+ + RER + R +II S+PDL+ER Sbjct: 57 EQYSEMLLNKMRESKETNPLFIVLDGYFYAADFFDE---HRERTVKRQKIIASHPDLEER 113 Query: 120 ELLKGQRLIASVTNSLAATYDSDLSELAARFAVGIFHMAFMKWIKSAEKGLAEQL 174 ELLK ++ + L Y+ + LA R A I+ +A+ +W+++ + L + L Sbjct: 114 ELLKQSKIEGLLVEYLLTDYEERTARLAVRLARAIYSVAWEEWLENETESLRQLL 168 >A2RHW6.1 A2RHW6_LACLM Transcriptional regulator, TetR family Length = 181 Score = 107 bits (266), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 62/175 (35%), Positives = 103/175 (58%), Gaps = 9/175 (5%) Query: 2 IKLKSKEKLQAAATKLFMEKGYENTTVQEIASLAGVTERTFFRQFKDKSDVLFDTNNELG 61 +K S+EKL AA +L +GY+NTTV +IA+ AG+TERTFFR+FK+K+D+ F G Sbjct: 1 MKKSSQEKLYEAALELITAQGYDNTTVLQIATKAGLTERTFFRKFKNKADIFFAG----G 56 Query: 62 QRVAAFITDNV--DVEKKPLKLAVEGFVSTSXXXXXXXGSRERVLARNQIIQSNPDLQER 119 ++ + + + + E PL + ++G+ + RER + R +II S+PDL+ER Sbjct: 57 EQYSEMLLNKMRESNETNPLFIVLDGYFYAADFFDE---HRERTVKRQKIIASHPDLEER 113 Query: 120 ELLKGQRLIASVTNSLAATYDSDLSELAARFAVGIFHMAFMKWIKSAEKGLAEQL 174 ELLK ++ + L Y+ + LA R A I+ +A+ +W+++ + L + L Sbjct: 114 ELLKQSKIEGLLVEYLLTDYEERTARLAVRLARAIYSVAWEEWLENETESLRQLL 168 >Q9CIW9.1 Q9CIW9_LACLA Transcriptional regulator Length = 181 Score = 104 bits (259), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 59/164 (35%), Positives = 95/164 (57%), Gaps = 5/164 (3%) Query: 2 IKLKSKEKLQAAATKLFMEKGYENTTVQEIASLAGVTERTFFRQFKDKSDVLFDTNNELG 61 +K S+EKL AA +L +GYENTTV +IA+ AG+TERTFFR+FK+K+D+ F + Sbjct: 1 MKKTSQEKLYEAALELITAQGYENTTVLQIATKAGLTERTFFRKFKNKADIFFAGGEQYS 60 Query: 62 QRVAAFITDNVDVEKKPLKLAVEGFVSTSXXXXXXXGSRERVLARNQIIQSNPDLQEREL 121 Q + + ++ PL + +E + + RER + R +II S+PDL+EREL Sbjct: 61 QMLLTKMKES--KANNPLAIVLESYFYAADFFDE---HRERTVKRQKIIASHPDLEEREL 115 Query: 122 LKGQRLIASVTNSLAATYDSDLSELAARFAVGIFHMAFMKWIKS 165 LK ++ + L Y + LA R A ++ +A+ +W+++ Sbjct: 116 LKQSKIEELLVEYLLTAYGERSARLAVRLARAVYSVAWEEWLEN 159 >Q5YRI3.1 Q5YRI3_NOCFA Putative transcriptional regulator Length = 201 Score = 97.1 bits (240), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 60/163 (36%), Positives = 85/163 (52%), Gaps = 3/163 (1%) Query: 6 SKEKLQAAATKLFMEKGYENTTVQEIASLAGVTERTFFRQFKDKSDVLFDTNNELGQRVA 65 + E+L+ AA LF G++ TTV EIA AGVTERTF+R F DK +VLF +L Q Sbjct: 8 ASERLRQAALDLFAANGFDATTVAEIARRAGVTERTFYRYFADKREVLFAGEEQLEQVFT 67 Query: 66 AFITDNVDVEKKPLKLAVEGFVSTSXXXXXXXGSRERVLARNQIIQSNPDLQERELLKGQ 125 +T PL + + R+ ARN +I +N LQERELLK Sbjct: 68 TAVTSA--PADAPLSRLLAAALEAGGRELQDRPGRDYARARNAVITANEQLQERELLKMA 125 Query: 126 RLIASVTNSLAATYDSDL-SELAARFAVGIFHMAFMKWIKSAE 167 +L ++T++ A ++ + + LA AV +F AF +WI E Sbjct: 126 KLSRALTSAFVARGNAPMAARLAGELAVSVFGTAFARWIAPGE 168 >Q2IK84.1 Q2IK84_ANADE Putative transcriptional regulator, TetR family Length = 207 Score = 92.0 bits (227), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 64/173 (36%), Positives = 95/173 (54%), Gaps = 10/173 (5%) Query: 6 SKEKLQAAATKLFMEKGYENTTVQEIASLAGVTERTFFRQFKDKSDVLFDTNNELGQRVA 65 ++ +L+ AA +L+ E+GY+ TTV EIA+ AG+TERTFFR F DK +VLF L QR+ Sbjct: 12 ARGRLERAAMELYRERGYDQTTVAEIAARAGLTERTFFRHFADKREVLFWGAAALRQRIV 71 Query: 66 AFITDNVDVEKKPLKLAVEGFVSTSXXXXXXXGSRERVLARNQ--IIQSNPDLQERELLK 123 I E PL + V ST+ R +AR + +I ++P+LQERE++K Sbjct: 72 HGIA-QAPAEAAPLDVVVGALESTAPLFEE-----RRAVARQRQALIAAHPELQEREVMK 125 Query: 124 GQRLIASVTNSLAA-TYDSDLSELAARFAVGIFHMAFMKWIKSAE-KGLAEQL 174 L A+ +L A + LAA + +F +AF W+ A+ +GL L Sbjct: 126 LTLLAAAAAEALRARGVAGPAAWLAAEAGMAVFKLAFEGWVGDAKGRGLPHHL 178 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.131 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 894,710,580 Number of extensions: 30307430 Number of successful extensions: 96288 Number of sequences better than 1.0e-05: 134 Number of HSP's gapped: 96379 Number of HSP's successfully gapped: 134 Length of query: 189 Length of database: 3,060,696,203 Length adjustment: 128 Effective length of query: 61 Effective length of database: 1,854,806,283 Effective search space: 113143183263 Effective search space used: 113143183263 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)