BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0028.p (307 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q032L3.1 Q032L3_LACLS Predicted nucleoside-diphosphate sugar epi... 301 6e-80 A2RHW4.1 A2RHW4_LACLM Putative uncharacterized protein 299 2e-79 Q88T91.1 Q88T91_LACPL Putative uncharacterized protein lp_3091 236 3e-60 C2FLM4.1 C2FLM4_LACPL NmrA family protein 236 3e-60 C2D333.1 C2D333_LACBR NmrA family protein 221 5e-56 >Q032L3.1 Q032L3_LACLS Predicted nucleoside-diphosphate sugar epimerase Length = 292 Score = 301 bits (770), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 152/294 (51%), Positives = 199/294 (67%), Gaps = 7/294 (2%) Query: 13 MKILVLGSLGNINRYYLPRLIADGHDVTVITSSSDRIADIEALGAKSAVGSNRDADFLTR 72 MKI +LGSLGNINR+++PRLIADGH+VTVITS + A IE LGAK+AVG N D DFL+R Sbjct: 1 MKITLLGSLGNINRHFIPRLIADGHEVTVITSRPEGAAAIEKLGAKAAVGLNTDLDFLSR 60 Query: 73 AFKGSDVVYLMISGINPASGIDMWQSAIELAENYKTAVQNSGVKNVVNLSSVGADNPNAG 132 F GSD+VYLMI+GI +S M +LA+ Y A++ + VK V+NLSSVGA +P AG Sbjct: 61 TFTGSDLVYLMITGIAFSSATSMS----DLAKIYSQAIKKADVKKVINLSSVGAQSPEAG 116 Query: 133 ILYMYHFAEDALNSLEDVNVAHIRPVGFYSNLFADMQSLKTMQTLFSPVSANIEHSWVSP 192 ILY YH ED L+ L V V HIRPVGFY+NL ++ LK +F +S +WV P Sbjct: 117 ILYEYHAMEDGLDELTGVTVKHIRPVGFYTNLLTEIALLKAQGKIFEAISPETMRAWVHP 176 Query: 193 IDIADVAYDLIDHTPAGKST---KFVVSDWATGNDWLKALAENGIEAKYQQIPIEALVEN 249 DIAD Y+ I+ K+ K+VVSDWATG DW+ AL N I A+Y I +E L+EN Sbjct: 177 SDIADAVYNRINDLLTDKAVSKIKYVVSDWATGTDWVNALGTNNISAQYVTISVEDLIEN 236 Query: 250 MIKLGFHEDTANRFAQMSRAGESPDEFYASLRATDYHLGKVKLEDFAKIFAAIY 303 +K G + A+ F QM++ +SPD+ Y +++++YH GKVK+ DFAK FA +Y Sbjct: 237 FVKAGLPKVVADGFGQMNQFQQSPDKLYQEIKSSNYHHGKVKINDFAKEFAQVY 290 >A2RHW4.1 A2RHW4_LACLM Putative uncharacterized protein Length = 292 Score = 299 bits (766), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 152/294 (51%), Positives = 198/294 (67%), Gaps = 7/294 (2%) Query: 13 MKILVLGSLGNINRYYLPRLIADGHDVTVITSSSDRIADIEALGAKSAVGSNRDADFLTR 72 MKI +LGSLGNINR+++PRLIADGH+VTVITS + A IE LGAK+AVG N D DFL+R Sbjct: 1 MKITLLGSLGNINRHFIPRLIADGHEVTVITSRPEGAAAIEKLGAKAAVGLNTDLDFLSR 60 Query: 73 AFKGSDVVYLMISGINPASGIDMWQSAIELAENYKTAVQNSGVKNVVNLSSVGADNPNAG 132 F GSD+VYLMI+GI +S M +LA+ Y A++ + VK V+NLSSVGA +P AG Sbjct: 61 TFTGSDLVYLMITGIAFSSATSMS----DLAKIYSQAIKKADVKKVINLSSVGAQSPEAG 116 Query: 133 ILYMYHFAEDALNSLEDVNVAHIRPVGFYSNLFADMQSLKTMQTLFSPVSANIEHSWVSP 192 ILY YH ED L+ L V V HIRPVGFY+NL ++ LK +F +S +WV P Sbjct: 117 ILYEYHAMEDGLDELTGVTVKHIRPVGFYTNLLTEIALLKAQGKIFEAISPETMRAWVHP 176 Query: 193 IDIADVAYDLIDHTPAGKST---KFVVSDWATGNDWLKALAENGIEAKYQQIPIEALVEN 249 DIAD Y+ I+ K+ K VVSDWATG DW+ AL N I A+Y I +E L+EN Sbjct: 177 SDIADAVYNRINDLLTDKAVSKIKHVVSDWATGTDWVNALGTNNISAQYVTISVEDLIEN 236 Query: 250 MIKLGFHEDTANRFAQMSRAGESPDEFYASLRATDYHLGKVKLEDFAKIFAAIY 303 +K G + A+ F QM++ +SPD+ Y +++++YH GKVK+ DFAK FA +Y Sbjct: 237 FVKAGLPKVVADGFGQMNQFQQSPDKLYQEIKSSNYHHGKVKINDFAKEFAQVY 290 >Q88T91.1 Q88T91_LACPL Putative uncharacterized protein lp_3091 Length = 304 Score = 236 bits (601), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 131/297 (44%), Positives = 180/297 (60%), Gaps = 6/297 (2%) Query: 13 MKILVLGSLGNINRYYLPRLIADGHDVTVITSSSDRIADIEALGAKSAVGSNRDADFLTR 72 MKI +LGSLGNINR +P+L+ GHDVTVI++S R A IEA+GA +A+G+ D DFL+ Sbjct: 1 MKIAMLGSLGNINRIVVPKLVQAGHDVTVISTSPKRQASIEAIGAHAAIGTMTDVDFLST 60 Query: 73 AFKGSDVVYLMISGINPASGIDMWQSAIELAENYKTAVQNSGVKNVVNLSSVGAD-NPNA 131 F DVVYLM+SG G D ++ A+ A+ +K A++N+GV NVVNLSS+GAD + A Sbjct: 61 TFTDKDVVYLMLSG---GIGDDPFEGAVAQAKIWKQAIENAGVTNVVNLSSIGADADEVA 117 Query: 132 GILYMYHFAEDALNSLEDVNVAHIRPVGFYSNLFADMQSLKTMQTLFSPVSANIEHSWVS 191 G L+ Y+ E L L+ VN+A +RP GFY+NLF ++ ++K ++S A +V+ Sbjct: 118 GSLHAYNLIEHELRQLDGVNLAFVRPTGFYANLFGNLATIKAEHKIYSSTPAASAQKYVA 177 Query: 192 PIDIADVAYDLIDHTPAGKSTKFVVSDWATGNDWLKAL--AENGIEAKYQQIPIEALVEN 249 P DIA V Y LIDHTPAG + K+ SD TG+ ++ L A N + ++ QI E N Sbjct: 178 PEDIASVVYPLIDHTPAGITVKYAFSDTFTGDQFVADLRDALNMPDLQWVQISDEQYQAN 237 Query: 250 MIKLGFHEDTANRFAQMSRAGESPDEFYASLRATDYHLGKVKLEDFAKIFAAIYKEE 306 + G A Q SR P+ YA L A+D G+VKL DF + F Y E Sbjct: 238 LTNHGVPVKIAASLVQTSRYQREPEGLYADLNASDTSAGQVKLADFVRTFVVAYNSE 294 >C2FLM4.1 C2FLM4_LACPL NmrA family protein Length = 304 Score = 236 bits (601), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 131/297 (44%), Positives = 180/297 (60%), Gaps = 6/297 (2%) Query: 13 MKILVLGSLGNINRYYLPRLIADGHDVTVITSSSDRIADIEALGAKSAVGSNRDADFLTR 72 MKI +LGSLGNINR +P+L+ GHDVTVI++S R A IEA+GA +A+G+ D DFL+ Sbjct: 1 MKIAMLGSLGNINRIVVPKLVQAGHDVTVISTSPKRQASIEAVGAHAAIGTMTDVDFLST 60 Query: 73 AFKGSDVVYLMISGINPASGIDMWQSAIELAENYKTAVQNSGVKNVVNLSSVGAD-NPNA 131 F DVVYLM+SG G D ++ A+ A+ +K A++N+GV NVVNLSS+GAD + A Sbjct: 61 TFTDKDVVYLMLSG---GIGDDPFEGAVAQAKIWKQAIENAGVTNVVNLSSIGADADEVA 117 Query: 132 GILYMYHFAEDALNSLEDVNVAHIRPVGFYSNLFADMQSLKTMQTLFSPVSANIEHSWVS 191 G L+ Y+ E L L+ VN+A +RP GFY+NLF ++ ++K ++S A +V+ Sbjct: 118 GSLHAYNLIEHELRQLDGVNLAFVRPTGFYANLFGNLATIKAEHKIYSSTPAASAQKYVA 177 Query: 192 PIDIADVAYDLIDHTPAGKSTKFVVSDWATGNDWLKAL--AENGIEAKYQQIPIEALVEN 249 P DIA V Y LIDHTPAG + K+ SD TG+ ++ L A N + ++ QI E N Sbjct: 178 PEDIASVVYPLIDHTPAGITVKYAFSDTFTGDQFVADLRDALNMPDLQWVQISDEQYQAN 237 Query: 250 MIKLGFHEDTANRFAQMSRAGESPDEFYASLRATDYHLGKVKLEDFAKIFAAIYKEE 306 + G A Q SR P+ YA L A+D G+VKL DF + F Y E Sbjct: 238 LTNHGVPVKIAASLVQTSRYQREPEGLYADLNASDTSAGQVKLADFVRTFVVAYNSE 294 >C2D333.1 C2D333_LACBR NmrA family protein Length = 305 Score = 221 bits (564), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 121/297 (40%), Positives = 177/297 (59%), Gaps = 5/297 (1%) Query: 13 MKILVLGSLGNINRYYLPRLIADGHDVTVITSSSDRIADIEALGAKSAVGSNRDADFLTR 72 MKI +LGSLG+I P L+ DGHDVT+IT+S R A I+ LGA +A+G+ D DFL+R Sbjct: 1 MKITLLGSLGHIGSIVAPALLKDGHDVTIITTSQKRAAQIKELGANAAIGTMTDEDFLSR 60 Query: 73 AFKGSDVVYLMISGINPASGIDMWQSAIELAENYKTAVQNSGVKNVVNLSSVGADNPNAG 132 F+ +DVVYLMISG + S ++++A + + + AV+ + VKNVV+LSS+GA P AG Sbjct: 61 QFQNADVVYLMISGNSDGS---IFENAKKQGQIFSNAVRKANVKNVVDLSSIGAYEPEAG 117 Query: 133 ILYMYHFAEDALNSLEDVNVAHIRPVGFYSNLFADMQSLKTMQTLFSPVSANIEHSWVSP 192 LY YHF ED L L DVNVA +RPVGFYSNL+ ++QS++ ++S + E VSP Sbjct: 118 SLYAYHFIEDKLRELNDVNVAFVRPVGFYSNLYTNVQSIRQNHVIYSNIPEQTEGKRVSP 177 Query: 193 IDIADVAYDLIDHTPAGKSTKFVVSDWATGNDWLKALAEN--GIEAKYQQIPIEALVENM 250 IDIA V L++ TP GK+ +V SD + + ++ L + + + QI + + Sbjct: 178 IDIAKVVLKLLEQTPEGKTIHYVYSDTFSMHQLIEMLKVSLPMPDLHFVQITDQEAENQL 237 Query: 251 IKLGFHEDTANRFAQMSRAGESPDEFYASLRATDYHLGKVKLEDFAKIFAAIYKEER 307 + G A F QM+ +P++ Y L G KL DF + F A + +++ Sbjct: 238 LSNGIPMSIAKSFVQMNAFQRNPEKLYEDLNRQSPVAGHYKLTDFVQEFTASFNDQK 294 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.317 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,789,469,505 Number of extensions: 72235589 Number of successful extensions: 205480 Number of sequences better than 1.0e-05: 94 Number of HSP's gapped: 205650 Number of HSP's successfully gapped: 94 Length of query: 307 Length of database: 3,060,696,203 Length adjustment: 135 Effective length of query: 172 Effective length of database: 1,788,859,178 Effective search space: 307683778616 Effective search space used: 307683778616 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)