BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0027.p (284 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value A5V0H0.1 A5V0H0_ROSS1 Short-chain dehydrogenase/reductase SDR 185 4e-45 C1Y143.1 C1Y143_9ACTO Putative uncharacterized protein 177 7e-43 Q07P18.1 Q07P18_RHOP5 Short-chain dehydrogenase/reductase SDR 172 4e-41 Q1DDZ7.1 Q1DDZ7_MYXXD Oxidoreductase, short chain dehydrogenase/... 171 5e-41 C3ZQD5.1 C3ZQD5_BRAFL Putative uncharacterized protein 169 2e-40 >A5V0H0.1 A5V0H0_ROSS1 Short-chain dehydrogenase/reductase SDR Length = 292 Score = 185 bits (469), Expect = 4e-45, Method: Compositional matrix adjust. Identities = 107/272 (39%), Positives = 161/272 (59%), Gaps = 7/272 (2%) Query: 13 VFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDISEATGNNRGDWVVADLS 72 + LI+G TSG+G+ A LAR G VVI+ RSAE A + I +ATG + ++ADLS Sbjct: 6 ICLITGATSGIGEVTARELARRGMHVVIVGRSAERVAATVARIKQATGVEI-ETLIADLS 64 Query: 73 LQSSIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVLSHYLLTNRMLD 132 Q+ +R V++ +++ +L VL+N G + ++++A+GI+ T+A+N +S++LLTN ++D Sbjct: 65 SQAGVRSVAEAFLQRHRRLDVLINNAGGFFASRQVSADGIELTWALNHMSYFLLTNLLID 124 Query: 133 ILQESGPARVITVSGNPRFFKNPVINFDDIQMERQYSGMSAANQSLFAKTLFAFELARRL 192 L+ S PARVI VS + + V+ +DD+ R Y+G +A QS A LF+ ELARRL Sbjct: 125 TLRASAPARVINVSSDAH--RGGVMRWDDLLFTRGYNGWAAYAQSKLANILFSNELARRL 182 Query: 193 EGTGVTSNAFYPGFVKSNLSRNAPWYLRAAAPLMNAWVKAECEIG----VYLASAKELER 248 EGTGVTSNA +PGFV + + N LM E G +YLA+A E+ Sbjct: 183 EGTGVTSNALHPGFVATRFAHNNGIIWGGLMALMQRLFAITPEEGAQTSIYLATAPEVAA 242 Query: 249 TNGVFFDDKKQTVPIYTKFDDGAGNKLWTLCE 280 +G +F ++T P D A +LW + E Sbjct: 243 ISGRYFVKSRETSPAPQAQDMAAAARLWEISE 274 >C1Y143.1 C1Y143_9ACTO Putative uncharacterized protein Length = 326 Score = 177 bits (450), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 104/261 (39%), Positives = 158/261 (60%), Gaps = 7/261 (2%) Query: 2 MNETRTDIENRVFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDISEATGN 61 MN R + + L++GGT G+G A ATGLA L A+V I+ RSA G AA + + Sbjct: 1 MN-ARDLMGGKTVLVTGGTGGIGLAAATGLAGLRARVGIVGRSAARGAAAADAVRSKVPS 59 Query: 62 NRGDWVVADLSLQSSIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVL 121 + D ADLS QS +R ++ +K Y +L VLVN G + + +TA+G++ TFA+N L Sbjct: 60 AQVDVFEADLSAQSEVRRLAAEVKATYSRLDVLVNNAGGYWSHRHVTADGLEHTFALNHL 119 Query: 122 SHYLLTNRMLDILQESGPARVITVSGNPRFFKNPVINFDDIQMERQYSGMSAANQSLFAK 181 + +LLT+ + D+L S PARV+TVS + I+FDD+Q ER Y+G A NQS A Sbjct: 120 APFLLTHELHDLLVASAPARVVTVSSGAQAMGR--IDFDDLQGERSYNGQRAYNQSKLAN 177 Query: 182 TLFAFELARRLEGTGVTSNAFYPGFVKSNLSR-NAPWYLRAAAPLMNAWVKAE---CEIG 237 LF +ELARRLEGTGVT+ +PG V+++ + ++ ++R PL+ ++K Sbjct: 178 VLFTYELARRLEGTGVTATVLHPGVVRTSFGQEDSDRWMRLVLPLVRPFMKTPDQGAATP 237 Query: 238 VYLASAKELERTNGVFFDDKK 258 +YLAS+ E++ +G +F ++ Sbjct: 238 IYLASSPEVDGVSGAYFAHQR 258 >Q07P18.1 Q07P18_RHOP5 Short-chain dehydrogenase/reductase SDR Length = 291 Score = 172 bits (435), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 113/285 (39%), Positives = 161/285 (56%), Gaps = 17/285 (5%) Query: 10 ENRVFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDISEATGNNRGDWVVA 69 E R L++G SG+GKAIAT LA GA+V ++ R G+AAL +I ATGN+ VA Sbjct: 7 EKRTALVTGANSGLGKAIATALAADGARVGLVVRDRARGEAALAEIRAATGNDDLHLFVA 66 Query: 70 DLSLQSSIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVLSHYLLTNR 129 DL+ Q++IR ++ ++ + QLH+LVN G + ++ L+ EGI+R A+N L +LLTN Sbjct: 67 DLADQAAIRALAQTVRNHFQQLHLLVNNAGTSFPERRLSPEGIERALAINHLGPFLLTNL 126 Query: 130 MLDILQESGPARVITVSGNPRFFKNPVINFDDIQMERQ-YSGMSAANQSLFAKTLFAFEL 188 +LD+L+ S PAR++ V + ++FDD+ ER+ Y M A QS F FEL Sbjct: 127 LLDLLKASAPARIVNVGTR----MDTAMDFDDLNWERRPYRMMQAYGQSKLGNLHFTFEL 182 Query: 189 ARRLEGTGVTSNAFYPGFVKSNL-----SRNAPWYLRAAAPLMNAWVKAECEIG----VY 239 ARRLEG+GVT N +PG KSNL ++ W L A L+ W E +Y Sbjct: 183 ARRLEGSGVTVNCVFPGVFKSNLGGTDGAQGLFWKLVA---LLLGWAIPSPEKAARRVLY 239 Query: 240 LASAKELERTNGVFFDDKKQTVPIYTKFDDGAGNKLWTLCEGLTS 284 +A+A EL +G +F D K D A +LW + E LT+ Sbjct: 240 VANAPELAEVSGEYFGDGKTIKAPAQARDPEANRRLWQISEALTA 284 >Q1DDZ7.1 Q1DDZ7_MYXXD Oxidoreductase, short chain dehydrogenase/reductase family Length = 280 Score = 171 bits (434), Expect = 5e-41, Method: Compositional matrix adjust. Identities = 101/278 (36%), Positives = 159/278 (57%), Gaps = 5/278 (1%) Query: 9 IENRVFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDISEATGNNRGDWVV 68 ++ +V LI+G T G+G A LAR+GA VV++ R A +AA+ + EA + + DW+ Sbjct: 1 MDGKVCLITGATGGIGLEAAKALARMGATVVLVGRDAGRTEAAVAAVKEAAPSAQVDWLR 60 Query: 69 ADLSLQSSIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVLSHYLLTN 128 ADL+ S+RE++ + +Y +L VL+N G I ++++T +G++ T A N + +LLTN Sbjct: 61 ADLASLKSVRELAQTFRSRYPRLDVLLNNAGLIIDRRQVTEDGLEATMATNHFAPFLLTN 120 Query: 129 RMLDILQESGPARVITVSGNPRFFKNPVINFDDIQMERQYSGMSAANQSLFAKTLFAFEL 188 +LD+++ +GPAR+I VS + ++FDD+Q ER + G S A LF L Sbjct: 121 LLLDVMKATGPARIINVSSDAHAAGK--LDFDDLQSERGFIGFRVYGTSKLANILFTRAL 178 Query: 189 ARRLEGTGVTSNAFYPGFVKSNLSRNAPWYLRAAAPLMNAW-VKAE--CEIGVYLASAKE 245 A+RLEGT VT+NA +PG V++ N + R L A+ + AE VYLAS+ E Sbjct: 179 AKRLEGTQVTTNALHPGVVRTGFGHNTQGFFRHLVKLGAAFMISAEKGARTSVYLASSPE 238 Query: 246 LERTNGVFFDDKKQTVPIYTKFDDGAGNKLWTLCEGLT 283 +E +G +F + P +D +LW + E LT Sbjct: 239 VEAVSGQYFYKCRPKKPSSAARNDALAERLWQVSEQLT 276 >C3ZQD5.1 C3ZQD5_BRAFL Putative uncharacterized protein Length = 323 Score = 169 bits (429), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 116/285 (40%), Positives = 154/285 (54%), Gaps = 17/285 (5%) Query: 9 IENRVFLISGGTSGVGKAIATGLARLGAKVVIISRSAESGQAALKDISEATGNNRGDWVV 68 ++ + +I+G G GK A LA GA+V++ R AA DI + TGN G+ VV Sbjct: 36 LDGKTVIITGANKGTGKETARALAAKGARVILACRDITKADAAANDIRQTTGN--GNVVV 93 Query: 69 ADLSLQS--SIREVSDVIKRKYDQLHVLVNACGAIYFKKELTAEGIDRTFAVNVLSHYLL 126 +L+L S SIR+ + IK++ L +L+N K +T +G +R FA N L H+LL Sbjct: 94 EELNLASLASIRKFAAKIKKRETSLDILINNAAVSACPKWVTEDGFERQFATNHLGHFLL 153 Query: 127 TNRMLDILQESGPARVITVSGNPRFFKNPVINFDDIQMERQYSGMSAANQSLFAKTLFAF 186 TN +LD+L+ S P+RVI VS +K INFDDI E+ YS A QS+ A LF Sbjct: 154 TNLLLDLLKTSAPSRVIVVSA--VLYKRGKINFDDINGEKSYSPHGAYCQSMLASVLFMR 211 Query: 187 ELARRLEGTGVTSNAFYPGFVKSNLSRNAP----WYLRAAAPLMNAWV-------KAECE 235 ELA+RLEGTGVT+NA +PG V + LSRN W + P AWV K + Sbjct: 212 ELAKRLEGTGVTANALHPGVVSTELSRNFSTTLGWIMLLLGPFFTAWVYLFAKTAKQGAQ 271 Query: 236 IGVYLASAKELERTNGVFFDDKKQTVPIYTKFDDGAGNKLWTLCE 280 V LA KELE T+G +F D K DD KLW + E Sbjct: 272 TTVRLAVDKELETTSGAYFSDCKPHELAPVGKDDVTARKLWQVSE 316 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,614,803,042 Number of extensions: 62976789 Number of successful extensions: 239422 Number of sequences better than 1.0e-05: 5900 Number of HSP's gapped: 243566 Number of HSP's successfully gapped: 5905 Length of query: 284 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 150 Effective length of database: 1,798,280,193 Effective search space: 269742028950 Effective search space used: 269742028950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)