BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0019.p (382 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q5WI80.1 Q5WI80_BACSK Epoxide hydrolase 1 EC=3.3.2.9 642 0.0 B3KER4.1 B3KER4_9BACI Epoxide hydrolase domain protein 639 0.0 A6LXC9.1 A6LXC9_CLOB8 Epoxide hydrolase domain protein 597 e-169 Q97L46.1 Q97L46_CLOAB Epoxide hydrolase, similar to eukaryotic 593 e-167 C2C455.1 C2C455_LISGR Possible microsomal epoxide hydrolase EC=3... 464 e-129 >Q5WI80.1 Q5WI80_BACSK Epoxide hydrolase 1 EC=3.3.2.9 Length = 385 Score = 642 bits (1655), Expect = 0.0, Method: Compositional matrix adjust. Identities = 295/381 (77%), Positives = 339/381 (88%) Query: 1 MAVEPFHIHVSDEILDDLQYKLHHIRWPDELENSGWDRGTDLSFLKSLVSYWRDHYDWRA 60 M +E F I VSDEIL+DLQY+++HIRWPD+LEN+ W+RGT+L++LKSLVSYWRDHYDWRA Sbjct: 1 MTIERFQIQVSDEILNDLQYRINHIRWPDQLENADWERGTELNYLKSLVSYWRDHYDWRA 60 Query: 61 QESMLNRFSQFRSNIDGIDVHFVHERGKGPNPLPIILTHGWPDSYLRYQKIIPLLSDPAS 120 QE+ LNRFSQFR IDGIDVHFVHERGKGP+PLP+ILTHGWPDS LRYQKIIPLL+DPAS Sbjct: 61 QEAKLNRFSQFRCKIDGIDVHFVHERGKGPDPLPLILTHGWPDSSLRYQKIIPLLTDPAS 120 Query: 121 YGGNPEDSFDVIVPSLPGFGFSGHPXXXXVNNFRVSQMWAELMTKELGFPKFAAAGGDMG 180 +GGNPEDSFDVIVPSLPGFGFS P VNNFRV++MWA+LMT++LG+ KFAAAGGDMG Sbjct: 121 HGGNPEDSFDVIVPSLPGFGFSSRPKHPRVNNFRVAEMWAKLMTEKLGYSKFAAAGGDMG 180 Query: 181 SGVTRYLAANRPELLYGIHLTDVGIIRSLLTLQDESECMEEERQYKKNASEWISQEGGYM 240 SGVTRYLAAN PE LYGIHLTD+GIIR L+ D+ EEE QYK AS W++QEGGYM Sbjct: 181 SGVTRYLAANHPERLYGIHLTDIGIIRDLIASSDQGTLSEEEWQYKNKASAWMAQEGGYM 240 Query: 241 AIQSTKPQTLAYGLSDSPVGLAAWIIEKFRTWSDCKGDLSQKYSKDELLTHIMIYWVTNT 300 +IQST+PQTLAYGLSDSPVGLA WI EKFR+WSDC GDL+QK+S+DELLTHIM+YWVTNT Sbjct: 241 SIQSTRPQTLAYGLSDSPVGLAGWITEKFRSWSDCNGDLAQKFSEDELLTHIMVYWVTNT 300 Query: 301 IGSTAHMYYENTHSLPPLGRIEVPTGIAIFPGDVLQPPKSWAVRNLNVTRWTTMPAGGHF 360 IGSTAHMYY+N HSLPP+G IEVPTG+A+FP D+L PPK WA+RNLNVTRWT+MP GGHF Sbjct: 301 IGSTAHMYYDNAHSLPPIGYIEVPTGLALFPADILLPPKEWAMRNLNVTRWTSMPRGGHF 360 Query: 361 TAMEEPEALANDIRAFYRSFR 381 TA+EEPE A+DIRAF+R FR Sbjct: 361 TALEEPELFADDIRAFFRPFR 381 >B3KER4.1 B3KER4_9BACI Epoxide hydrolase domain protein Length = 386 Score = 639 bits (1648), Expect = 0.0, Method: Compositional matrix adjust. Identities = 294/381 (77%), Positives = 342/381 (89%) Query: 1 MAVEPFHIHVSDEILDDLQYKLHHIRWPDELENSGWDRGTDLSFLKSLVSYWRDHYDWRA 60 M++E +HIHVSDEIL DL+Y+LHHIRWPD+LE+SGW+RGT+LS+LKSLVSYWRDHYDWRA Sbjct: 1 MSMERYHIHVSDEILADLKYRLHHIRWPDQLEHSGWERGTELSYLKSLVSYWRDHYDWRA 60 Query: 61 QESMLNRFSQFRSNIDGIDVHFVHERGKGPNPLPIILTHGWPDSYLRYQKIIPLLSDPAS 120 QE+ LNR+SQ+ NID IDVHFVHERGKGPNP+PIILTHGWPDSYLRYQKIIPLL+DPAS Sbjct: 61 QEAELNRYSQYSCNIDDIDVHFVHERGKGPNPMPIILTHGWPDSYLRYQKIIPLLTDPAS 120 Query: 121 YGGNPEDSFDVIVPSLPGFGFSGHPXXXXVNNFRVSQMWAELMTKELGFPKFAAAGGDMG 180 +GG+PEDSFDV+VPSLPGFGFS P NN RVS+MWA+LMTKELG+ KFAAAGGD+G Sbjct: 121 HGGHPEDSFDVVVPSLPGFGFSSSPKQPGFNNNRVSEMWAKLMTKELGYKKFAAAGGDIG 180 Query: 181 SGVTRYLAANRPELLYGIHLTDVGIIRSLLTLQDESECMEEERQYKKNASEWISQEGGYM 240 SGVTRYLAAN PELL+GIHLTD+GIIR+L+ ++ +EE +YK +ASEWI+ EGGYM Sbjct: 181 SGVTRYLAANHPELLFGIHLTDIGIIRNLMNASGQANLSQEELRYKTSASEWIALEGGYM 240 Query: 241 AIQSTKPQTLAYGLSDSPVGLAAWIIEKFRTWSDCKGDLSQKYSKDELLTHIMIYWVTNT 300 +IQST+PQTLAYGLSDSP GLA W+IEKFR+WSDCKG+L Q++SKDELLTHIMIYWVTNT Sbjct: 241 SIQSTRPQTLAYGLSDSPAGLAGWMIEKFRSWSDCKGNLQQRFSKDELLTHIMIYWVTNT 300 Query: 301 IGSTAHMYYENTHSLPPLGRIEVPTGIAIFPGDVLQPPKSWAVRNLNVTRWTTMPAGGHF 360 +GS+ MYYEN+HSLPPLG IEVPTGIA+FP D+L PPK WA RNLNVTRWT+MP+GGHF Sbjct: 301 MGSSTRMYYENSHSLPPLGYIEVPTGIALFPEDILLPPKEWASRNLNVTRWTSMPSGGHF 360 Query: 361 TAMEEPEALANDIRAFYRSFR 381 TAMEEPE LA DIRAFY+ +R Sbjct: 361 TAMEEPELLARDIRAFYKPYR 381 >A6LXC9.1 A6LXC9_CLOB8 Epoxide hydrolase domain protein Length = 380 Score = 597 bits (1538), Expect = e-169, Method: Compositional matrix adjust. Identities = 270/381 (70%), Positives = 332/381 (87%), Gaps = 1/381 (0%) Query: 1 MAVEPFHIHVSDEILDDLQYKLHHIRWPDELENSGWDRGTDLSFLKSLVSYWRDHYDWRA 60 M+++ F+I VSDE+L+DL+Y+L HIRWP+ LEN W+RGT++S+LKSLVSYW+D +DWRA Sbjct: 1 MSIKKFNIQVSDEVLNDLKYRLDHIRWPELLENLSWERGTNISYLKSLVSYWKDEFDWRA 60 Query: 61 QESMLNRFSQFRSNIDGIDVHFVHERGKGPNPLPIILTHGWPDSYLRYQKIIPLLSDPAS 120 QE LNRFS F N+DGID+HF+HE+GKGPNP PIILTHGWPDS++RYQKIIPLL+DPAS Sbjct: 61 QEKELNRFSHFCCNVDGIDIHFIHEKGKGPNPTPIILTHGWPDSFIRYQKIIPLLTDPAS 120 Query: 121 YGGNPEDSFDVIVPSLPGFGFSGHPXXXXVNNFRVSQMWAELMTKELGFPKFAAAGGDMG 180 YGG+P DSFDVI+PSLPGFGFS +NN+RVS++WA+LMT++LG+ KFAAAGGDMG Sbjct: 121 YGGDPNDSFDVIIPSLPGFGFSSASKHSGMNNYRVSELWAKLMTEKLGYSKFAAAGGDMG 180 Query: 181 SGVTRYLAANRPELLYGIHLTDVGIIRSLLTLQDESECMEEERQYKKNASEWISQEGGYM 240 SGVTRYLA N PELL GIHLTD+GIIR L+ + +++E ++E YKK ASEW+S E GYM Sbjct: 181 SGVTRYLALNHPELLIGIHLTDIGIIRDLM-MSNDTELSKDELNYKKIASEWMSNEAGYM 239 Query: 241 AIQSTKPQTLAYGLSDSPVGLAAWIIEKFRTWSDCKGDLSQKYSKDELLTHIMIYWVTNT 300 +IQSTKPQT+AYGLSDSPVGLAAWIIEKF +WSDC GDL++ +KDELLT+IMIYWVTNT Sbjct: 240 SIQSTKPQTIAYGLSDSPVGLAAWIIEKFLSWSDCNGDLNKILTKDELLTNIMIYWVTNT 299 Query: 301 IGSTAHMYYENTHSLPPLGRIEVPTGIAIFPGDVLQPPKSWAVRNLNVTRWTTMPAGGHF 360 IGS+A+ YYENTHSLPP+G I+VPTG+A+FP DVL PPK WA+RNLN+TRWTTMP GGHF Sbjct: 300 IGSSANAYYENTHSLPPMGHIDVPTGMALFPADVLLPPKEWAMRNLNITRWTTMPKGGHF 359 Query: 361 TAMEEPEALANDIRAFYRSFR 381 TAME+P+ A +IR F+R++R Sbjct: 360 TAMEDPKHFAEEIRTFFRTYR 380 >Q97L46.1 Q97L46_CLOAB Epoxide hydrolase, similar to eukaryotic Length = 381 Score = 593 bits (1528), Expect = e-167, Method: Compositional matrix adjust. Identities = 272/381 (71%), Positives = 331/381 (86%) Query: 1 MAVEPFHIHVSDEILDDLQYKLHHIRWPDELENSGWDRGTDLSFLKSLVSYWRDHYDWRA 60 M+++ F+I VSDE+L++L+ +L +++WPD+LE+ G++RGTDL++LKSLVSYW + +DWRA Sbjct: 1 MSIKRFNIKVSDEVLNNLKSRLKNVKWPDQLEDLGFERGTDLNYLKSLVSYWIEDFDWRA 60 Query: 61 QESMLNRFSQFRSNIDGIDVHFVHERGKGPNPLPIILTHGWPDSYLRYQKIIPLLSDPAS 120 QE+ LN FSQF IDGID+HF+HE GKGPNP+PIILTHGWPDSY+RY+K+IPLL+DPA Sbjct: 61 QETELNSFSQFHCKIDGIDIHFIHEHGKGPNPIPIILTHGWPDSYIRYKKLIPLLTDPAK 120 Query: 121 YGGNPEDSFDVIVPSLPGFGFSGHPXXXXVNNFRVSQMWAELMTKELGFPKFAAAGGDMG 180 YGGNPEDSFDVIVPSLPGFGFSG P +NN VS++WA+LMT++LG+ KFAAAGGD+G Sbjct: 121 YGGNPEDSFDVIVPSLPGFGFSGKPKYGGMNNSNVSELWAKLMTEKLGYNKFAAAGGDVG 180 Query: 181 SGVTRYLAANRPELLYGIHLTDVGIIRSLLTLQDESECMEEERQYKKNASEWISQEGGYM 240 SGVTRYLA N PELL GIHLTDVGII++L+T Q + EE QYKKNAS+WIS+EG YM Sbjct: 181 SGVTRYLALNHPELLIGIHLTDVGIIKALMTSQGNLKLSGEEVQYKKNASKWISEEGAYM 240 Query: 241 AIQSTKPQTLAYGLSDSPVGLAAWIIEKFRTWSDCKGDLSQKYSKDELLTHIMIYWVTNT 300 +IQSTKPQTLAYGLSDSPVGLAAWIIEKFR WSDCKG+LS+ ++ DELLT+IMIYWVTNT Sbjct: 241 SIQSTKPQTLAYGLSDSPVGLAAWIIEKFRAWSDCKGNLSRSFNMDELLTNIMIYWVTNT 300 Query: 301 IGSTAHMYYENTHSLPPLGRIEVPTGIAIFPGDVLQPPKSWAVRNLNVTRWTTMPAGGHF 360 IGS+ ++YYENTHSLP L I+VPTG+AIFP D+L PPK W RNLN+TRWTTMP GGHF Sbjct: 301 IGSSVNIYYENTHSLPSLSYIDVPTGMAIFPADILLPPKEWVERNLNITRWTTMPRGGHF 360 Query: 361 TAMEEPEALANDIRAFYRSFR 381 TAMEEPE LA +IRAF++ +R Sbjct: 361 TAMEEPELLAEEIRAFFKPYR 381 >C2C455.1 C2C455_LISGR Possible microsomal epoxide hydrolase EC=3.3.2.9 Length = 383 Score = 464 bits (1194), Expect = e-129, Method: Compositional matrix adjust. Identities = 206/378 (54%), Positives = 279/378 (73%) Query: 3 VEPFHIHVSDEILDDLQYKLHHIRWPDELENSGWDRGTDLSFLKSLVSYWRDHYDWRAQE 62 +E F I ++D++L DL+++L +RWP +LE GW++GT+ FL+ L YW + YDWR QE Sbjct: 6 LEVFKISITDDVLADLRHRLELVRWPSQLEAIGWEQGTERHFLQKLTDYWLNDYDWRKQE 65 Query: 63 SMLNRFSQFRSNIDGIDVHFVHERGKGPNPLPIILTHGWPDSYLRYQKIIPLLSDPASYG 122 ++LN+F Q+++ IDG+ +HFV+E+G+ +P+ILTHGWPDSYLRYQK+IPLL++P YG Sbjct: 66 ALLNQFQQYKTTIDGLSIHFVYEKGQTSRRIPLILTHGWPDSYLRYQKVIPLLTNPRRYG 125 Query: 123 GNPEDSFDVIVPSLPGFGFSGHPXXXXVNNFRVSQMWAELMTKELGFPKFAAAGGDMGSG 182 + + SFD+IVPS+PGFGFS P NN V+ +W +LMT+ LG+ KF A+GGDMGSG Sbjct: 126 IDSDLSFDIIVPSMPGFGFSEVPKQAGFNNASVADLWVKLMTETLGYEKFVASGGDMGSG 185 Query: 183 VTRYLAANRPELLYGIHLTDVGIIRSLLTLQDESECMEEERQYKKNASEWISQEGGYMAI 242 VTRYLAA E L GIHLTDVG+I++L+ + E+ Y + S+W+ E GYMAI Sbjct: 186 VTRYLAAKYSEQLLGIHLTDVGLIQALIQKSGSQIENKSEQTYVETVSKWLRDEAGYMAI 245 Query: 243 QSTKPQTLAYGLSDSPVGLAAWIIEKFRTWSDCKGDLSQKYSKDELLTHIMIYWVTNTIG 302 QSTKPQTLAYGLSDSPVGLA W++EK+R+WSDC GDL +++S DE LTH+MIYWVTNTI Sbjct: 246 QSTKPQTLAYGLSDSPVGLAGWLLEKYRSWSDCDGDLRKRFSNDEFLTHVMIYWVTNTIA 305 Query: 303 STAHMYYENTHSLPPLGRIEVPTGIAIFPGDVLQPPKSWAVRNLNVTRWTTMPAGGHFTA 362 S+ MY N+H+LP LG I +PT I++F D+ PPK+W R NV +W+ P GGHFTA Sbjct: 306 SSIRMYDANSHTLPKLGTITIPTSISLFSADINLPPKAWVERAFNVQQWSVNPKGGHFTA 365 Query: 363 MEEPEALANDIRAFYRSF 380 EEPE + ++ F ++ Sbjct: 366 FEEPEWFSKELFRFAKTL 383 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,617,183,426 Number of extensions: 117858386 Number of successful extensions: 292269 Number of sequences better than 1.0e-05: 505 Number of HSP's gapped: 290570 Number of HSP's successfully gapped: 520 Length of query: 382 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 245 Effective length of database: 1,770,017,148 Effective search space: 433654201260 Effective search space used: 433654201260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)