BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0018.p (358 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value Q1IMR1.1 Q1IMR1_ACIBL NAD-dependent epimerase/dehydratase 284 9e-75 B4W1K8.1 B4W1K8_9CYAN NAD dependent epimerase/dehydratase family 275 7e-72 O06485.2 YFNG_BACSU Putative sugar dehydratase/epimerase yfnG 267 1e-69 B0BZZ2.1 B0BZZ2_ACAM1 NAD-dependent epimerase/dehydratase 263 3e-68 A7Z2B2.1 A7Z2B2_BACA2 YfnG 258 4e-67 >Q1IMR1.1 Q1IMR1_ACIBL NAD-dependent epimerase/dehydratase Length = 324 Score = 284 bits (726), Expect = 9e-75, Method: Compositional matrix adjust. Identities = 138/319 (43%), Positives = 212/319 (66%), Gaps = 9/319 (2%) Query: 26 DCRALVTGASGFVGAWLTHHLIEQGAEVTSVLSEFNPRSQFAKLGLDKKIRCYYGSIADY 85 D LVTGA+G +G WLT HL+EQGA VT+++ + P+S+F + + +++ G ++ Sbjct: 8 DRSVLVTGATGLLGGWLTRHLLEQGASVTALVRDSVPQSEFERCLMRQRVNVVQGDLSKP 67 Query: 86 NLIERAITDGKINTIFHLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQDQISKII 145 L+ER + + ++ T+FHLAA ++ +A + P TFE+N++GT+NLLE CR +S I+ Sbjct: 68 QLLERVLGEYEVETVFHLAAQTIVGIANRNPVSTFESNIRGTWNLLEACR-RSPNVSAIV 126 Query: 146 VASSDKVYGDSPDLPYDEGMPIQGRNPYDASKSCSDLISQSYRHSFNLPIVIGRFGNIFG 205 +ASSDK YGD LPY E MP+QGR+PYD SKSC+DLI+QSY H+F +P+ I R GN +G Sbjct: 127 LASSDKAYGDQTVLPYTEDMPLQGRHPYDVSKSCADLIAQSYAHTFRVPVAITRCGNFYG 186 Query: 206 GGDLNFRRLIPGTIQRLYAQLPPIIRIPSNGXXXXXXXXFLYIQDLMAAYMAMYHY--VN 263 GGDLN+ R++PGTI+ ++ PIIR S+G + YI+D AAYM + V+ Sbjct: 187 GGDLNWNRVVPGTIRSVFRGERPIIR--SDG---KFVRDYFYIEDGAAAYMLLAERLTVD 241 Query: 264 NEGTGNVFNFGTGKLWDIKKVIEIIQRVMNLEHIEPLYEHQKNSEILHQYLSTEKAKSAL 323 + G+ FNF ++ ++ I + MN +++P ++Q N+EI HQ+LS E+A+ L Sbjct: 242 KKLIGSAFNFSNEAQINVLDLVNTILQKMN-SNLKPEIQNQANNEIRHQFLSAERARKQL 300 Query: 324 NWSAETSLEEGIRKTVDWY 342 NW A+ +L+EG+ +T+ WY Sbjct: 301 NWRAQYTLDEGLERTIAWY 319 >B4W1K8.1 B4W1K8_9CYAN NAD dependent epimerase/dehydratase family Length = 334 Score = 275 bits (702), Expect = 7e-72, Method: Compositional matrix adjust. Identities = 137/327 (41%), Positives = 207/327 (63%), Gaps = 7/327 (2%) Query: 20 MKSSLSDCRALVTGASGFVGAWLTHHLIEQGAEVTSVLSEFNPRSQFAKLGLDKKIRCYY 79 M S D VTG +G +G+W+T L+ +GA V ++ ++ P+S+ GL KKI Y Sbjct: 1 MDSFWQDRSVFVTGCTGLLGSWMTQELVNRGANVVGLVRDWVPQSRLFTEGLSKKIVTVY 60 Query: 80 GSIADYNLIERAITDGKINTIFHLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQD 139 G + D +ER +++ +I+T+FHLAA ++ +A + P TFE N+KGT+NLLE CR Sbjct: 61 GRVEDIATLERTLSEYEIDTVFHLAAQTIVGVANREPLGTFEANIKGTWNLLEACR-RVG 119 Query: 140 QISKIIVASSDKVYGDSPDLPYDEGMPIQGRNPYDASKSCSDLISQSYRHSFNLPIVIGR 199 +S+I+VASSDK YGD LPYDE P+QG +PYD SKSC+DLI ++Y S+ LP+ + R Sbjct: 120 GVSRIVVASSDKAYGDQEILPYDETTPLQGEHPYDVSKSCADLICRTYFVSYKLPVCVTR 179 Query: 200 FGNIFGGGDLNFRRLIPGTIQRLYAQLPPIIRIPSNGXXXXXXXXFLYIQDLMAAYMAMY 259 GN +GGGDLNF R++PGTI+ + PIIR S+G + Y++D + AY+ + Sbjct: 180 CGNFYGGGDLNFNRIVPGTIRSILRGERPIIR--SDG---SYIRDYFYVKDGVLAYLHLA 234 Query: 260 HYVNNEGT-GNVFNFGTGKLWDIKKVIEIIQRVMNLEHIEPLYEHQKNSEILHQYLSTEK 318 + + G FNF + +++E I +M+ + +EPL +Q +EI+HQYLS +K Sbjct: 235 EQMERQEIWGEAFNFSNELQITVLELVEKIIGLMDRKDLEPLILNQVKNEIIHQYLSAQK 294 Query: 319 AKSALNWSAETSLEEGIRKTVDWYHNY 345 A+ LNW E SL+E +++T+DWY + Sbjct: 295 ARRLLNWEPEYSLDEALKETIDWYRRF 321 >O06485.2 YFNG_BACSU Putative sugar dehydratase/epimerase yfnG Length = 322 Score = 267 bits (682), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 138/318 (43%), Positives = 205/318 (64%), Gaps = 8/318 (2%) Query: 30 LVTGASGFVGAWLTHHLIEQGAEVTSVLSEFNPRSQFAKLGLDKKIRCYYGSIADYNLIE 89 VTG +G +G++L LIEQGA VT ++ + P+S + KK+ GS+ D +IE Sbjct: 10 FVTGCTGLLGSYLVKELIEQGANVTGLVRDHVPQSNLYQGEHIKKMNIVRGSLEDLAVIE 69 Query: 90 RAITDGKINTIFHLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQDQISKIIVASS 149 RA+ + +I+T+FHLAA ++ +A + P TFE N+ GT+N+LE CR H I ++IVASS Sbjct: 70 RALGEYEIDTVFHLAAQAIVGVANRNPISTFEANILGTWNILEACRKH-PLIKRVIVASS 128 Query: 150 DKVYGDSPDLPYDEGMPIQGRNPYDASKSCSDLISQSYRHSFNLPIVIGRFGNIFGGGDL 209 DK YGD +LPYDE MP+QG++PYD SKSC+DLIS +Y H++ LP+ I R GN++GGGDL Sbjct: 129 DKAYGDQENLPYDENMPLQGKHPYDVSKSCADLISHTYFHTYGLPVCITRCGNLYGGGDL 188 Query: 210 NFRRLIPGTIQRLYAQLPPIIRIPSNGXXXXXXXXFLYIQDLMAAYMAMYHYV-NNEGTG 268 NF R+IP TIQ + P IR S+G + YI+D + AY+ + + N G Sbjct: 189 NFNRIIPQTIQLVLNGEAPEIR--SDG---TFVRDYFYIEDAVQAYLLLAEKMEENNLAG 243 Query: 269 NVFNFGTGKLWDIKKVIEIIQRVMNLEHIEPLYEHQKNSEILHQYLSTEKAKSALNWSAE 328 FNF + +++E I + MN +++P +Q ++EI HQYLS EKA+ LNW+ Sbjct: 244 EAFNFSNEIQLTVLELVEKILKKMN-SNLKPKVLNQGSNEIKHQYLSAEKARKLLNWTPA 302 Query: 329 TSLEEGIRKTVDWYHNYW 346 +++EG+ KT++WY ++ Sbjct: 303 YTIDEGLEKTIEWYTEFF 320 >B0BZZ2.1 B0BZZ2_ACAM1 NAD-dependent epimerase/dehydratase Length = 327 Score = 263 bits (671), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 132/332 (39%), Positives = 202/332 (60%), Gaps = 7/332 (2%) Query: 20 MKSSLSDCRALVTGASGFVGAWLTHHLIEQGAEVTSVLSEFNPRSQFAKLGLDKKIRCYY 79 M D L+TG +G +G+W+T L+ +GA V ++ ++ P+S+ GL KI Y Sbjct: 1 MSDFWQDRSVLITGCTGLLGSWMTQELVARGARVVGLVRDWVPQSRLFTEGLSDKITTVY 60 Query: 80 GSIADYNLIERAITDGKINTIFHLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQD 139 G I D ++ERAI + +++T+FHLAA ++ +A + P TFE N+KGT+N+LE CR Sbjct: 61 GGIEDLGVLERAINEYEVDTVFHLAAQTIVGVANREPLGTFEANIKGTWNVLEACR-RVG 119 Query: 140 QISKIIVASSDKVYGDSPDLPYDEGMPIQGRNPYDASKSCSDLISQSYRHSFNLPIVIGR 199 +S+I+VASSDK YGD LPYDE P+QG +PYD SKSC+DL+ ++Y ++NLP+ I R Sbjct: 120 GVSRIVVASSDKAYGDQEILPYDEQTPLQGEHPYDVSKSCADLLCRTYYVTYNLPVCITR 179 Query: 200 FGNIFGGGDLNFRRLIPGTIQRLYAQLPPIIRIPSNGXXXXXXXXFLYIQDLMAAYMAMY 259 GN +GGGDLNF R++P TI+ P +IR S+G + Y++D + AY+ + Sbjct: 180 CGNFYGGGDLNFNRIVPDTIRSTLRDKPIVIR--SDG---TYIRDYFYVKDGVLAYLHLA 234 Query: 260 HYVNN-EGTGNVFNFGTGKLWDIKKVIEIIQRVMNLEHIEPLYEHQKNSEILHQYLSTEK 318 ++ E G FNF + +++ I ++M H+EP +Q +EI HQYLS EK Sbjct: 235 EQMDRPEILGEAFNFSNELQISVLELVHKILKLMGKPHLEPTILNQAQNEIKHQYLSAEK 294 Query: 319 AKSALNWSAETSLEEGIRKTVDWYHNYWNSDS 350 A+ L+W + L+ + +T+ WY + S S Sbjct: 295 ARRLLDWKSPYDLDAALLETIQWYTAFLESTS 326 >A7Z2B2.1 A7Z2B2_BACA2 YfnG Length = 322 Score = 258 bits (660), Expect = 4e-67, Method: Compositional matrix adjust. Identities = 132/318 (41%), Positives = 203/318 (63%), Gaps = 8/318 (2%) Query: 30 LVTGASGFVGAWLTHHLIEQGAEVTSVLSEFNPRSQFAKLGLDKKIRCYYGSIADYNLIE 89 VTG +G +G+ L LI+QGA VT ++ + P+S + K++ G++ D ++IE Sbjct: 10 FVTGCTGLLGSCLVKELIDQGANVTGLVRDTVPKSNLYQGEQVKQMNIVQGALEDLDVIE 69 Query: 90 RAITDGKINTIFHLAAVSLQDLAYQIPWQTFETNVKGTYNLLEVCRIHQDQISKIIVASS 149 RA+ + +I+T+FHLAA ++ +A + P TFE N+ GT+N+LE CR H I ++IVASS Sbjct: 70 RALGEYEIDTVFHLAAQAIVGVANRNPISTFEANILGTWNILEACRRHP-LIKRVIVASS 128 Query: 150 DKVYGDSPDLPYDEGMPIQGRNPYDASKSCSDLISQSYRHSFNLPIVIGRFGNIFGGGDL 209 DK YGD P LPYDE MP+QG++PYD SKSC+DL+S +Y +++ LP+ I R GN++GGGDL Sbjct: 129 DKAYGDQPTLPYDENMPLQGKHPYDVSKSCADLLSHTYFNTYGLPVCITRCGNLYGGGDL 188 Query: 210 NFRRLIPGTIQRLYAQLPPIIRIPSNGXXXXXXXXFLYIQDLMAAYMAMYHYVNNEG-TG 268 NF R+IP TIQ + P IR S+G + YI+D + AY+ + + G Sbjct: 189 NFNRIIPQTIQLVLNGEAPEIR--SDG---TFIRDYFYIEDAVEAYLLLAEKMEELNLAG 243 Query: 269 NVFNFGTGKLWDIKKVIEIIQRVMNLEHIEPLYEHQKNSEILHQYLSTEKAKSALNWSAE 328 FNF + +++E I + M+ + ++P +Q + EI HQYLS EKA+ LNW+ Sbjct: 244 EAFNFSNEIQLTVLELVEKILKAMDSD-LKPKVLNQGSHEIKHQYLSAEKARKLLNWTPA 302 Query: 329 TSLEEGIRKTVDWYHNYW 346 +++EG+ KT++WY ++ Sbjct: 303 HTIDEGLEKTIEWYKAFF 320 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,141,905,366 Number of extensions: 87973143 Number of successful extensions: 253988 Number of sequences better than 1.0e-05: 7037 Number of HSP's gapped: 242801 Number of HSP's successfully gapped: 7071 Length of query: 358 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 221 Effective length of database: 1,770,017,148 Effective search space: 391173789708 Effective search space used: 391173789708 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)