BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0012.p (660 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5S0.1 B3K5S0_9BACI PTS system, trehalose-specific IIBC subunit 846 0.0 B7GI26.1 B7GI26_ANOFW Phosphotransferase system (PTS) trehalose-... 608 e-172 Q2B448.1 Q2B448_9BACI PTS system, trehalose-specific enzyme II, ... 578 e-163 C5DAU6.1 C5DAU6_9BACI PTS system, trehalose-specific IIBC subunit 577 e-162 C3IZJ8.1 C3IZJ8_9BACI PTS system, trehalose-specific IIBC subunit 572 e-161 >B3K5S0.1 B3K5S0_9BACI PTS system, trehalose-specific IIBC subunit Length = 678 Score = 846 bits (2185), Expect = 0.0, Method: Compositional matrix adjust. Identities = 425/639 (66%), Positives = 502/639 (78%), Gaps = 12/639 (1%) Query: 1 MAINRQNVEEIVKAVGGKENIEAATHCVTRLRFALHDESQVDTDALEKNDLVKGHFSSQG 60 MAI+R+NVE IV+AVGGK+NIEAATHCVTRLRFAL D+S+VD+ AL++NDLVKG FSSQG Sbjct: 1 MAIDRKNVERIVEAVGGKDNIEAATHCVTRLRFALVDDSKVDSRALDENDLVKGQFSSQG 60 Query: 61 QFQVIIGPGLVDKVYDEMLSITGGSRATKDEVKTAASKKQNPLQQAIKTLADIFIPILPA 120 QFQV+IGPGLVD+VY+EM+ ITGG+R +KDEVK ASKKQNPLQ+AIKTLADIFIPILPA Sbjct: 61 QFQVVIGPGLVDQVYNEMMDITGGARVSKDEVKNIASKKQNPLQRAIKTLADIFIPILPA 120 Query: 121 IVMAXXXXXXXXXXTGPGIFFDSKSLVEVYPQWKDFASIINTIASTAFTFLPALIGWSAV 180 IVMA TGPGIF++ +SL++V+P+W D ASIIN IASTAFTFLP LIGWSAV Sbjct: 121 IVMAGLLLGINNLLTGPGIFYEGQSLIQVHPEWADVASIINLIASTAFTFLPVLIGWSAV 180 Query: 181 TRFGGSPXXXXXXXXXXXHPDLLSAYGYADAKVEGTVPVWDLFGWHVNKIGYQGQVLPVL 240 +FGG+P +PDLLSAY YADAK+EGTVPVW+LFG+ V KIGYQGQVLPVL Sbjct: 181 RQFGGNPLLGIVLGLILVNPDLLSAYQYADAKLEGTVPVWNLFGFEVEKIGYQGQVLPVL 240 Query: 241 VSAYLLARLERWLNQKVPDSFKLLIVAPVALLITGFLAFTVIGPITFVIANAITSGLVSI 300 VSAY+LA++E++LN++V DS KLL+VAPVALL+TGFLAF ++GPITF I N +TSGLVSI Sbjct: 241 VSAYILAKIEKFLNKRVHDSIKLLVVAPVALLVTGFLAFVLVGPITFAIGNVLTSGLVSI 300 Query: 301 FGGFPXXXXXXXXXXXXXXVITGMHHTFLAVDIQLIGSEGGTFLWPMLALSNIAQGAAAL 360 F F VITGMHHTFLAVDIQLIGSEGGTFLWPMLALSNIAQGAAAL Sbjct: 301 FDSFAALGGLIYGGLYALLVITGMHHTFLAVDIQLIGSEGGTFLWPMLALSNIAQGAAAL 360 Query: 361 GMMFVAKQEKTKGLAVTSSISAFLGVTEPAIFGVNVRFKYPFIFGMLGSAIAGVLLAMNN 420 MMF+ K++K KGLAVTSSISAFLGVTEPAIFGVNVR KYPFIFGM+GS IAGVLLA+NN Sbjct: 361 AMMFIMKEQKAKGLAVTSSISAFLGVTEPAIFGVNVRHKYPFIFGMIGSGIAGVLLAVNN 420 Query: 421 VLASSIGVGGIPGFLSIFPSQWGVFFIGMAIVLIVPFTATVLFGKVR----------SRG 470 VLASSIGVGGIPGFLSIFP+QWGVFFIGMAIVL+VPF T+LFG+ + + G Sbjct: 421 VLASSIGVGGIPGFLSIFPNQWGVFFIGMAIVLVVPFAGTLLFGRYQLSKQSRHATAADG 480 Query: 471 XXXXXXXXXXXXXXXXXXEPSAPSHAQVHTDSVPVSSPATAVNVLEVLSPVRGRIVPLEQ 530 + S A T + P + A +EV +P++G++VPL + Sbjct: 481 NAAGIDGRAQAVNTESNGSLQSGSFASTETAN-PSTVEAEQQGAVEVKAPIKGKVVPLSE 539 Query: 531 VPDPAFAERQMGKGVAVEPFEGKVTAPFDATVAHVI-KSKHAVILEHASGVQLLIHIGIN 589 VPDPAFA++QMG+G+A+ P EGKV APFD VAH+I KSKHA+IL A+G+Q+LIH+GIN Sbjct: 540 VPDPAFADKQMGQGIAIIPSEGKVYAPFDGQVAHLIKKSKHAIILTDANGIQILIHVGIN 599 Query: 590 TVSLKGNGFTSHVAAGDKVKAGQLLLEFDRDAIAAGGFP 628 TVSLKG GFT+HV G +++ GQLLLEFD D I G P Sbjct: 600 TVSLKGEGFTAHVETGAEIRKGQLLLEFDIDRIQQAGLP 638 >B7GI26.1 B7GI26_ANOFW Phosphotransferase system (PTS) trehalose-specific enzyme II, BC component Length = 467 Score = 608 bits (1569), Expect = e-172, Method: Compositional matrix adjust. Identities = 297/463 (64%), Positives = 363/463 (78%) Query: 5 RQNVEEIVKAVGGKENIEAATHCVTRLRFALHDESQVDTDALEKNDLVKGHFSSQGQFQV 64 RQ+VE+IV+AVGGKENI AATHCVTRLRFAL DE +VD +ALE D+VKG FS GQFQV Sbjct: 2 RQSVEQIVQAVGGKENIIAATHCVTRLRFALKDEGKVDKEALENIDIVKGSFSVNGQFQV 61 Query: 65 IIGPGLVDKVYDEMLSITGGSRATKDEVKTAASKKQNPLQQAIKTLADIFIPILPAIVMA 124 +IG GLVDKVY+EM+ +TG RATK E+K AA KK NPLQ+AIKTLADIFIPILPAIV A Sbjct: 62 VIGQGLVDKVYNEMVEMTGIGRATKQEIKDAAEKKLNPLQRAIKTLADIFIPILPAIVTA 121 Query: 125 XXXXXXXXXXTGPGIFFDSKSLVEVYPQWKDFASIINTIASTAFTFLPALIGWSAVTRFG 184 TGPGIF++ KS+V+V+ QW+DFA +IN IA+TAF FLP LIGWSAV +FG Sbjct: 122 GLLMGINNILTGPGIFYEGKSIVDVHEQWRDFADMINLIANTAFVFLPGLIGWSAVKKFG 181 Query: 185 GSPXXXXXXXXXXXHPDLLSAYGYADAKVEGTVPVWDLFGWHVNKIGYQGQVLPVLVSAY 244 GS HP LL+A+ YA A EGTVP W+LFG+ V KIGYQGQVLP+L+S+Y Sbjct: 182 GSELLGIVLGLMLVHPALLNAWAYASAVQEGTVPYWNLFGFDVQKIGYQGQVLPILISSY 241 Query: 245 LLARLERWLNQKVPDSFKLLIVAPVALLITGFLAFTVIGPITFVIANAITSGLVSIFGGF 304 +LA+LE +L +++P++F+LL+VAP+ALL+TGF++F IGP+TF IANA+T GLV++F F Sbjct: 242 VLAKLEVFLKRRIPEAFQLLLVAPIALLLTGFISFIAIGPVTFGIANALTDGLVALFDRF 301 Query: 305 PXXXXXXXXXXXXXXVITGMHHTFLAVDIQLIGSEGGTFLWPMLALSNIAQGAAALGMMF 364 V+TGMHHTFLAVD+QLIGS GGTFLWPMLALSNIAQG+AAL MMF Sbjct: 302 AALGGLVYGGLYAVLVVTGMHHTFLAVDLQLIGSTGGTFLWPMLALSNIAQGSAALAMMF 361 Query: 365 VAKQEKTKGLAVTSSISAFLGVTEPAIFGVNVRFKYPFIFGMLGSAIAGVLLAMNNVLAS 424 K EK KGL+ TS++SA+LG+TEPA+FGVN+RF+YPFI ++GSA AG+ + +N V AS Sbjct: 362 ATKDEKLKGLSFTSAVSAYLGITEPAMFGVNLRFRYPFISALIGSATAGIFITINKVKAS 421 Query: 425 SIGVGGIPGFLSIFPSQWGVFFIGMAIVLIVPFTATVLFGKVR 467 SIGVGG+PGFLSIFP +W FFIGMAIVLIVPFT T + K + Sbjct: 422 SIGVGGLPGFLSIFPDKWVPFFIGMAIVLIVPFTLTYILAKTK 464 >Q2B448.1 Q2B448_9BACI PTS system, trehalose-specific enzyme II, BC component Length = 467 Score = 578 bits (1490), Expect = e-163, Method: Compositional matrix adjust. Identities = 291/467 (62%), Positives = 345/467 (73%), Gaps = 3/467 (0%) Query: 1 MAINRQNVEEIVKAVGGKENIEAATHCVTRLRFALHDESQVDTDALEKNDLVKGHFSSQG 60 M+ R++ E IV+AVGGKENI AATHCVTRLRFAL DES+VD +ALE D VKG FS+ G Sbjct: 1 MSNYRESAERIVEAVGGKENIAAATHCVTRLRFALKDESKVDKEALENIDSVKGSFSTNG 60 Query: 61 QFQVIIGPGLVDKVYDEMLSITGGSRATKDEVKTAASKKQNPLQQAIKTLADIFIPILPA 120 QFQV+IG GLVDKVY EM + TG +K+EVK AAS+K NPLQ+AIKTLADIFIPILPA Sbjct: 61 QFQVVIGQGLVDKVYKEMAAATGIGELSKEEVKAAASEKMNPLQRAIKTLADIFIPILPA 120 Query: 121 IVMAXXXXXXXXXXTGPGIFFDSKSLVEVYPQWKDFASIINTIASTAFTFLPALIGWSAV 180 IV A T P IF+ KS ++V+P W D A+IIN IA+TAF FLP LIGWSAV Sbjct: 121 IVTAGLLMGINNIMTAPDIFYTGKSFIDVHPGWADLANIINMIANTAFVFLPGLIGWSAV 180 Query: 181 TRFGGSPXXXXXXXXXXXHPDLLSAYGYADAKVEGTVPVWDLFGWHVNKIGYQGQVLPVL 240 RFGGSP HPDLL+A+GY VPVW+LFG + K+GYQGQVLPVL Sbjct: 181 NRFGGSPLLGMVLGLMLVHPDLLNAWGYGSTD---KVPVWNLFGLEIEKVGYQGQVLPVL 237 Query: 241 VSAYLLARLERWLNQKVPDSFKLLIVAPVALLITGFLAFTVIGPITFVIANAITSGLVSI 300 ++Y+LAR+E +L +++PDS +LL VAP+ALLITGFL+F IGP+TF I IT G+VSI Sbjct: 238 AASYVLARIEIFLRKRIPDSIQLLTVAPIALLITGFLSFIAIGPVTFTIGGWITDGVVSI 297 Query: 301 FGGFPXXXXXXXXXXXXXXVITGMHHTFLAVDIQLIGSEGGTFLWPMLALSNIAQGAAAL 360 F P VITGMHHTFLAVD+QLIGS GGTFLWPM+ALSNIAQG+A+L Sbjct: 298 FDAVPALGGLIYGGLYAPLVITGMHHTFLAVDLQLIGSIGGTFLWPMVALSNIAQGSASL 357 Query: 361 GMMFVAKQEKTKGLAVTSSISAFLGVTEPAIFGVNVRFKYPFIFGMLGSAIAGVLLAMNN 420 M F+ K EK KGLA TSS+SAFLGVTEPA+FGVN+RFKYPFI ++GSA+ G+ + + Sbjct: 358 AMFFLNKNEKVKGLAATSSLSAFLGVTEPAMFGVNLRFKYPFIAAVIGSALGGMFITIKG 417 Query: 421 VLASSIGVGGIPGFLSIFPSQWGVFFIGMAIVLIVPFTATVLFGKVR 467 V+A SIGVGG+P F SI WG FFIGMAIVL+VPF T LFGK R Sbjct: 418 VIAPSIGVGGLPAFFSILSENWGSFFIGMAIVLVVPFVLTFLFGKFR 464 >C5DAU6.1 C5DAU6_9BACI PTS system, trehalose-specific IIBC subunit Length = 471 Score = 577 bits (1486), Expect = e-162, Method: Compositional matrix adjust. Identities = 290/469 (61%), Positives = 358/469 (76%) Query: 1 MAINRQNVEEIVKAVGGKENIEAATHCVTRLRFALHDESQVDTDALEKNDLVKGHFSSQG 60 M Q+V +IV+A+GGKENI AATHCVTRLRFAL DE +VD + LE D+VKG FS+ G Sbjct: 1 MGAYEQSVAKIVEAIGGKENIVAATHCVTRLRFALKDEGKVDKEKLESIDIVKGSFSANG 60 Query: 61 QFQVIIGPGLVDKVYDEMLSITGGSRATKDEVKTAASKKQNPLQQAIKTLADIFIPILPA 120 QFQV+IG GLVDKVY+EM+ +TG RATK E+K AA K NPLQ+AIKTLADIFIPILPA Sbjct: 61 QFQVVIGQGLVDKVYNEMVEMTGIGRATKQEIKDAAEAKLNPLQRAIKTLADIFIPILPA 120 Query: 121 IVMAXXXXXXXXXXTGPGIFFDSKSLVEVYPQWKDFASIINTIASTAFTFLPALIGWSAV 180 IV A TGPGIF++ KS VEV+ +W D AS+IN IA+TAF FLP LIGWSAV Sbjct: 121 IVTAGLLMGINNILTGPGIFYEGKSFVEVHKEWADLASMINLIANTAFVFLPGLIGWSAV 180 Query: 181 TRFGGSPXXXXXXXXXXXHPDLLSAYGYADAKVEGTVPVWDLFGWHVNKIGYQGQVLPVL 240 T+FGGSP HPDLL+A+G+ AK +G +P+W+LFG+ V K+GYQGQVLPVL Sbjct: 181 TKFGGSPLLGIVLGLMLVHPDLLNAWGWGAAKEKGEIPIWNLFGFEVQKVGYQGQVLPVL 240 Query: 241 VSAYLLARLERWLNQKVPDSFKLLIVAPVALLITGFLAFTVIGPITFVIANAITSGLVSI 300 V++Y+LA++E++L +++PD+F+LL+VAP+ALLITGFLAF IGPITF I NAIT+ VSI Sbjct: 241 VASYVLAKIEQFLRKRIPDAFQLLLVAPLALLITGFLAFIAIGPITFAIGNAITNVFVSI 300 Query: 301 FGGFPXXXXXXXXXXXXXXVITGMHHTFLAVDIQLIGSEGGTFLWPMLALSNIAQGAAAL 360 F P V+TGMHHTFL VD+QLI S GGTFLWP+L +SN+AQG+AAL Sbjct: 301 FDNVPAIGGFLYGALYAPLVVTGMHHTFLPVDLQLIASTGGTFLWPILVMSNVAQGSAAL 360 Query: 361 GMMFVAKQEKTKGLAVTSSISAFLGVTEPAIFGVNVRFKYPFIFGMLGSAIAGVLLAMNN 420 MMF AK EK KGL+ TS++SA+LG+TEPA+FGVN+RF+YPFI M G+AIAG+ + +N Sbjct: 361 AMMFAAKDEKLKGLSFTSAVSAYLGITEPAMFGVNLRFRYPFISAMTGAAIAGMFITLNK 420 Query: 421 VLASSIGVGGIPGFLSIFPSQWGVFFIGMAIVLIVPFTATVLFGKVRSR 469 V+A SIGVGG+PGFLSI P +W FFIGMAI +IVPF T +F K R Sbjct: 421 VIAPSIGVGGLPGFLSIVPQKWAPFFIGMAIAIIVPFALTFVFSKFRKE 469 >C3IZJ8.1 C3IZJ8_9BACI PTS system, trehalose-specific IIBC subunit Length = 472 Score = 572 bits (1474), Expect = e-161, Method: Compositional matrix adjust. Identities = 286/468 (61%), Positives = 361/468 (77%) Query: 1 MAINRQNVEEIVKAVGGKENIEAATHCVTRLRFALHDESQVDTDALEKNDLVKGHFSSQG 60 M +Q +IV+A+GGKENI AATHCVTRLRFAL +E +V+ +ALE+ D+VKG FS+ G Sbjct: 1 MGAYQQAAAQIVEAIGGKENIVAATHCVTRLRFALKEEGKVNKEALERIDVVKGSFSANG 60 Query: 61 QFQVIIGPGLVDKVYDEMLSITGGSRATKDEVKTAASKKQNPLQQAIKTLADIFIPILPA 120 QFQV+IG GLVDKVYD+++ +TG RATK E+K AA K NPLQ+AIKTLADIFIPILPA Sbjct: 61 QFQVVIGQGLVDKVYDDLIELTGIGRATKQEIKDAAEAKLNPLQRAIKTLADIFIPILPA 120 Query: 121 IVMAXXXXXXXXXXTGPGIFFDSKSLVEVYPQWKDFASIINTIASTAFTFLPALIGWSAV 180 IV A TGPGIF++ KS VEV+ +W D A++IN IA+TAF FLP LIGWSAV Sbjct: 121 IVTAGLLMGINNVLTGPGIFYEGKSFVEVHKEWADLANMINLIANTAFVFLPGLIGWSAV 180 Query: 181 TRFGGSPXXXXXXXXXXXHPDLLSAYGYADAKVEGTVPVWDLFGWHVNKIGYQGQVLPVL 240 T+FGGSP HPDLL+A+G+ AK +G +PVW+LFG+ V K+GYQGQVLPVL Sbjct: 181 TKFGGSPLLGIVLGLMLVHPDLLNAWGWGAAKEKGEIPVWNLFGFEVQKVGYQGQVLPVL 240 Query: 241 VSAYLLARLERWLNQKVPDSFKLLIVAPVALLITGFLAFTVIGPITFVIANAITSGLVSI 300 V++Y+LA+LE +L +++PD+F+LL+VAP+ALLITGFLAF IGP+TF I NAIT V+I Sbjct: 241 VASYVLAKLELFLRKRIPDAFQLLLVAPLALLITGFLAFIAIGPVTFAIGNAITHLFVTI 300 Query: 301 FGGFPXXXXXXXXXXXXXXVITGMHHTFLAVDIQLIGSEGGTFLWPMLALSNIAQGAAAL 360 F P V+TGMHHTFL VD+QLI + GGTFLWP+L +SNIAQG+AAL Sbjct: 301 FDTVPALGGLLYGALYAPLVVTGMHHTFLPVDLQLIANTGGTFLWPILVMSNIAQGSAAL 360 Query: 361 GMMFVAKQEKTKGLAVTSSISAFLGVTEPAIFGVNVRFKYPFIFGMLGSAIAGVLLAMNN 420 MMFVAK+EK +GL+ TS++SA+LG+TEPA+FGVN+RF++PFI M G+AIAG+ + +N Sbjct: 361 AMMFVAKEEKLRGLSFTSAVSAYLGITEPAMFGVNLRFRFPFIAAMTGAAIAGMFITLNK 420 Query: 421 VLASSIGVGGIPGFLSIFPSQWGVFFIGMAIVLIVPFTATVLFGKVRS 468 V+A SIGVGG+PGFLSI P +W FFIGMAI ++VPF T +FGKVR Sbjct: 421 VIAPSIGVGGLPGFLSIVPQKWTPFFIGMAIAIVVPFALTFVFGKVRK 468 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.321 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 3,474,465,148 Number of extensions: 136569188 Number of successful extensions: 360666 Number of sequences better than 1.0e-05: 3030 Number of HSP's gapped: 353604 Number of HSP's successfully gapped: 3438 Length of query: 660 Length of database: 3,060,696,203 Length adjustment: 143 Effective length of query: 517 Effective length of database: 1,713,491,058 Effective search space: 885874876986 Effective search space used: 885874876986 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 135 (56.6 bits)