BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0544.0011.p (378 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3K5S1.1 B3K5S1_9BACI Alpha,alpha-phosphotrehalase 521 e-146 A4INV3.1 A4INV3_GEOTN Alpha-glucosidase 443 e-122 B4BSG9.1 B4BSG9_9BACI Alpha,alpha-phosphotrehalase 443 e-122 C5DAU7.1 C5DAU7_9BACI Alpha,alpha-phosphotrehalase 441 e-122 Q3MUG8.1 Q3MUG8_BACST Trehalose-6-phosphate hydrolase 439 e-121 >B3K5S1.1 B3K5S1_9BACI Alpha,alpha-phosphotrehalase Length = 571 Score = 521 bits (1342), Expect = e-146, Method: Compositional matrix adjust. Identities = 242/377 (64%), Positives = 291/377 (77%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 + FWA+KGV GFRMDVINLISKD ++ RRFYTDGPRVHEYIKEL KVF Sbjct: 192 LTFWADKGVSGFRMDVINLISKDPAYPDDDGSVAPGDGRRFYTDGPRVHEYIKELNEKVF 251 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 PYNLVTVGEMSSTTLEHCIRYS P+E EFSMTFNFHHLKVDY NGQKWEL+PYDF ELK Sbjct: 252 KPYNLVTVGEMSSTTLEHCIRYSNPKENEFSMTFNFHHLKVDYPNGQKWELKPYDFEELK 311 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 + + WQ GMQ+ GGWNALFWNNHDQPRAL+RF +DG+YR ESAKMLATTLHG+QGTPYV Sbjct: 312 RVLSDWQIGMQKGGGWNALFWNNHDQPRALTRFTNDGEYRSESAKMLATTLHGLQGTPYV 371 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEE+GMP+P W+DISEF D+ES NMY ILQE+G + E+A I+R RSRDNSR PM W Sbjct: 372 YQGEEIGMPDPKWNDISEFRDVESLNMYTILQEQGRTPEEAAHIVRVRSRDNSRLPMPWH 431 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 AGFT+GTPW+K+D RY IN Q+E +P SI+ HY++LI +RK PV+TDG + RL Sbjct: 432 DGDQAGFTTGTPWIKLDERYPLINAQSEVDNPQSIYHHYRKLIEMRKNLPVMTDGEYVRL 491 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 DEGHP+I+AYAR +TL+V+S+FS F ++ + S++ +LLIGNT + P Sbjct: 492 DEGHPEIYAYARTNKEQTLVVISNFSDRDVSFELPEKMKDNLTESRSVQLLIGNTTEEPK 551 Query: 361 WSQSLEIPPYGSYMWII 377 ++ + PY SYMW+I Sbjct: 552 LMPTVALRPYASYMWLI 568 >A4INV3.1 A4INV3_GEOTN Alpha-glucosidase Length = 563 Score = 443 bits (1140), Expect = e-122, Method: Compositional matrix adjust. Identities = 209/378 (55%), Positives = 273/378 (72%), Gaps = 3/378 (0%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 M FW +KGVDGFR+DVINL+SKDQ F RRFYTDGPR+HE+++E+ +VF Sbjct: 187 MHFWLKKGVDGFRLDVINLLSKDQHFPDDDGSIPPGDGRRFYTDGPRIHEFLQEMNREVF 246 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 Y+++TVGEMSSTT++HCIRY+ PE E +MTFNFHHLKVDY NG+KW + P+DF LK Sbjct: 247 SKYDVMTVGEMSSTTIDHCIRYTNPENNELNMTFNFHHLKVDYPNGEKWAVAPFDFLALK 306 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 + + WQ M + GGWNALFW NHDQPR +SR+ DDG+Y KESAKMLATT+H MQGTPY+ Sbjct: 307 RILSEWQVRMHEGGGWNALFWCNHDQPRIVSRYGDDGEYWKESAKMLATTIHLMQGTPYI 366 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEE+GM +P ++DI ++ D+ES NMYRILQE+G S ++ L+I+R +SRDNSRTPMQWD Sbjct: 367 YQGEEIGMTDPKFNDIGDYRDVESLNMYRILQEQGKSEQEVLEILRRKSRDNSRTPMQWD 426 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 S HAGFTSGTPW++V Y+ INV+ D DSIF HYK+LI LRKQ +IT G ++ L Sbjct: 427 DSLHAGFTSGTPWIRVADNYRYINVKQALADRDSIFYHYKRLIELRKQYDIITTGRYELL 486 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 P +FAY R G+ E L+VV++F + F+ +E + N ELLI N P Sbjct: 487 LADDPHLFAYMRHGDGEKLLVVNNFYPVETTFTLPEE---AEANDYTGELLIANYPDAPA 543 Query: 361 WSQSLEIPPYGSYMWIIR 378 + + + PY S ++++R Sbjct: 544 DFRRMALRPYESVVYLLR 561 >B4BSG9.1 B4BSG9_9BACI Alpha,alpha-phosphotrehalase Length = 563 Score = 443 bits (1140), Expect = e-122, Method: Compositional matrix adjust. Identities = 209/378 (55%), Positives = 273/378 (72%), Gaps = 3/378 (0%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 M FW +KGVDGFR+DVINL+SKDQ F RRFYTDGPR+HE+++E+ +VF Sbjct: 187 MHFWLKKGVDGFRLDVINLLSKDQHFPDDDGSIPPGDGRRFYTDGPRIHEFLQEMNREVF 246 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 Y+++TVGEMSSTT++HCIRY+ PE E +MTFNFHHLKVDY NG+KW + P+DF LK Sbjct: 247 SKYDVMTVGEMSSTTIDHCIRYTNPENNELNMTFNFHHLKVDYPNGEKWAVAPFDFLALK 306 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 + + WQ M + GGWNALFW NHDQPR +SR+ DDG+Y KESAKMLATT+H MQGTPY+ Sbjct: 307 RILSEWQVRMHEGGGWNALFWCNHDQPRIVSRYGDDGEYWKESAKMLATTIHLMQGTPYI 366 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEE+GM +P ++DI ++ D+ES NMYRILQE+G S ++ L+I+R +SRDNSRTPMQWD Sbjct: 367 YQGEEIGMTDPKFNDIGDYRDVESLNMYRILQEQGKSEQEVLEILRRKSRDNSRTPMQWD 426 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 S HAGFTSGTPW++V Y+ INV+ D DSIF HYK+LI LRKQ +IT G ++ L Sbjct: 427 DSLHAGFTSGTPWIRVADNYRYINVKQALADRDSIFYHYKRLIELRKQYDIITTGRYELL 486 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 P +FAY R G+ E L+VV++F + F+ +E + N ELLI N P Sbjct: 487 LADDPHLFAYMRHGDGEKLLVVNNFYPVETTFTLPEE---AEANDYTGELLIANYPDAPA 543 Query: 361 WSQSLEIPPYGSYMWIIR 378 + + + PY S ++++R Sbjct: 544 DFRRMALRPYESVVYLLR 561 >C5DAU7.1 C5DAU7_9BACI Alpha,alpha-phosphotrehalase Length = 563 Score = 441 bits (1133), Expect = e-122, Method: Compositional matrix adjust. Identities = 202/377 (53%), Positives = 275/377 (72%), Gaps = 3/377 (0%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 M FW +KGVDGFR+DVINL+SKDQ F R+FYTDGPR+HE+++E+ +VF Sbjct: 187 MHFWFQKGVDGFRLDVINLLSKDQRFLDDDGSVPPGDGRKFYTDGPRIHEFLQEMNREVF 246 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 Y+++TVGEMSSTT++HCI+Y+ P RE +MTFNFHHLKVDY NG+KW + +DF LK Sbjct: 247 SKYDIMTVGEMSSTTIDHCIKYTNPNRRELNMTFNFHHLKVDYPNGEKWAVADFDFLSLK 306 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 + + WQ M + GGWNALFW NHDQPR +SR+ DDGKY KESAKMLAT +H MQGTPY+ Sbjct: 307 RILSEWQVKMHKGGGWNALFWCNHDQPRIVSRYGDDGKYHKESAKMLATVIHMMQGTPYI 366 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEE+GM +P + I ++ D+ES NMYRILQE+G S ++ L+I++ +SRDNSRTPMQWD Sbjct: 367 YQGEEIGMTDPKFERIDDYRDVESLNMYRILQEQGKSEQEVLEILKRKSRDNSRTPMQWD 426 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 S HAGFT+GTPW++V YK+INV+ DP S+F HY++LI+LRKQ +IT G ++ L Sbjct: 427 DSEHAGFTTGTPWIRVAPNYKQINVKKALEDPTSVFYHYQRLIQLRKQYDIITTGDYQLL 486 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 E HP IFAY R G E L+VV++F G + F D+ ++ ++E+L+ N + +P Sbjct: 487 LEDHPDIFAYLRNGENEKLLVVNNFYGRETTFVLPDDV---DVSGYSSEILLSNYDDSPS 543 Query: 361 WSQSLEIPPYGSYMWII 377 +++ + PY S ++ + Sbjct: 544 DFRTISLRPYESIVYYL 560 >Q3MUG8.1 Q3MUG8_BACST Trehalose-6-phosphate hydrolase Length = 563 Score = 439 bits (1129), Expect = e-121, Method: Compositional matrix adjust. Identities = 201/377 (53%), Positives = 272/377 (72%), Gaps = 3/377 (0%) Query: 1 MIFWAEKGVDGFRMDVINLISKDQSFXXXXXXXXXXXXRRFYTDGPRVHEYIKELYAKVF 60 M FW +KG+DGFR+DVINL+SKDQ R FYTDGPR+HE+++E+ +VF Sbjct: 187 MHFWFQKGIDGFRLDVINLLSKDQRILDDDGSVPPGDGREFYTDGPRIHEFLQEMNREVF 246 Query: 61 GPYNLVTVGEMSSTTLEHCIRYSAPEEREFSMTFNFHHLKVDYKNGQKWELQPYDFAELK 120 Y+++TVGEMSSTT++HCI+Y+ PE +E +MTFNFHHLKVDY NG+KW + +DF LK Sbjct: 247 SKYDIMTVGEMSSTTIDHCIKYTNPERKELNMTFNFHHLKVDYPNGEKWAVADFDFLALK 306 Query: 121 ELFTRWQTGMQQAGGWNALFWNNHDQPRALSRFADDGKYRKESAKMLATTLHGMQGTPYV 180 + + WQ M + GGWNALFW NHDQPR +SR+ DDGKY KESAKMLAT +H MQGTPY+ Sbjct: 307 RILSTWQVEMHKGGGWNALFWCNHDQPRIVSRYGDDGKYHKESAKMLATVIHMMQGTPYI 366 Query: 181 YQGEELGMPNPDWHDISEFNDIESKNMYRILQERGLSGEQALDIIRERSRDNSRTPMQWD 240 YQGEE+GM +P + I ++ D+ES NMY+ILQE+G S E+ LDI++ +SRDNSRTPMQWD Sbjct: 367 YQGEEIGMTDPKFERIEDYRDVESLNMYKILQEKGKSKEEVLDILKRKSRDNSRTPMQWD 426 Query: 241 ASGHAGFTSGTPWLKVDRRYKEINVQAEQIDPDSIFAHYKQLIRLRKQEPVITDGIFKRL 300 S HAGFT+GTPW++V YK+INV+ DP SIF HY++LI+LRKQ +IT G ++ L Sbjct: 427 DSEHAGFTTGTPWIRVASNYKDINVKNALADPTSIFYHYQRLIQLRKQYDIITTGDYQLL 486 Query: 301 DEGHPQIFAYARVGNTETLIVVSSFSGTQAGFSFADEFWSSRINSQAAELLIGNTEQTPV 360 E HP IFAY R G E L+VV++F G + F E ++ +++L+ N E +P Sbjct: 487 LEDHPDIFAYIRNGKNEKLLVVNNFYGKETTFILPSEV---DVSGYTSQILLSNYEDSPE 543 Query: 361 WSQSLEIPPYGSYMWII 377 +++ + PY + ++ + Sbjct: 544 QFKTIVLRPYEAIVYYL 560 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,389,987,749 Number of extensions: 100951649 Number of successful extensions: 226537 Number of sequences better than 1.0e-05: 1891 Number of HSP's gapped: 222155 Number of HSP's successfully gapped: 1920 Length of query: 378 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 241 Effective length of database: 1,770,017,148 Effective search space: 426574132668 Effective search space used: 426574132668 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)