BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0544.0010.p
         (89 letters)

Database: pfamseq 
           9,421,015 sequences; 3,060,696,203 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

B3K5S1.1 B3K5S1_9BACI Alpha,alpha-phosphotrehalase                    137   2e-31
Q9L872.1 Q9L872_9BACI Alpha-glucosidase                               124   2e-27
Q5KZ55.1 Q5KZ55_GEOKA Alpha,alpha-phosphotrehalase EC=3.2.1.93        124   3e-27
C5DAU7.1 C5DAU7_9BACI Alpha,alpha-phosphotrehalase                    124   4e-27
C3IZJ9.1 C3IZJ9_9BACI Alpha,alpha-phosphotrehalase                    121   2e-26

>B3K5S1.1 B3K5S1_9BACI Alpha,alpha-phosphotrehalase
          Length = 571

 Score =  137 bits (346), Expect = 2e-31,   Method: Compositional matrix adjust.
 Identities = 62/77 (80%), Positives = 69/77 (89%)

Query: 1   MEDFEELIRELKKRGMHLMTDIVVNHSSTEHPWFQEARKSKDNPYRNYYIWRDPAPDGGV 60
           M+DF+EL+ ELKKR MHLM DIVVNHSSTEH WF+EA  S+DNPYR+YYIWRDPA DGG 
Sbjct: 84  MDDFDELVAELKKRDMHLMIDIVVNHSSTEHRWFKEAISSRDNPYRDYYIWRDPAEDGGP 143

Query: 61  PNNWQSKFGGPAWQFDE 77
           PNNWQSKFGGPAWQ+DE
Sbjct: 144 PNNWQSKFGGPAWQYDE 160


>Q9L872.1 Q9L872_9BACI Alpha-glucosidase
          Length = 562

 Score =  124 bits (312), Expect = 2e-27,   Method: Compositional matrix adjust.
 Identities = 52/77 (67%), Positives = 65/77 (84%)

Query: 1   MEDFEELIRELKKRGMHLMTDIVVNHSSTEHPWFQEARKSKDNPYRNYYIWRDPAPDGGV 60
           MEDF+ L+ E+ +RGM L+ D+VVNH+STEH WF++AR SKDNPYR++YIWRDP PDG  
Sbjct: 78  MEDFDRLLEEVHRRGMKLIMDMVVNHTSTEHEWFKQARTSKDNPYRHFYIWRDPKPDGSA 137

Query: 61  PNNWQSKFGGPAWQFDE 77
           P NWQSKFGG AW++DE
Sbjct: 138 PTNWQSKFGGSAWEYDE 154


>Q5KZ55.1 Q5KZ55_GEOKA Alpha,alpha-phosphotrehalase EC=3.2.1.93
          Length = 563

 Score =  124 bits (311), Expect = 3e-27,   Method: Compositional matrix adjust.
 Identities = 52/77 (67%), Positives = 65/77 (84%)

Query: 1   MEDFEELIRELKKRGMHLMTDIVVNHSSTEHPWFQEARKSKDNPYRNYYIWRDPAPDGGV 60
           M+DF+ L+ E+  RGM L+ D+VVNH+ST+H WFQ+AR SK NPYR++YIWRDP PDGG 
Sbjct: 79  MDDFDRLLHEVHARGMKLVMDMVVNHTSTDHEWFQQARSSKTNPYRSFYIWRDPKPDGGA 138

Query: 61  PNNWQSKFGGPAWQFDE 77
           PNNWQSKFGG AW++DE
Sbjct: 139 PNNWQSKFGGSAWEYDE 155


>C5DAU7.1 C5DAU7_9BACI Alpha,alpha-phosphotrehalase
          Length = 563

 Score =  124 bits (310), Expect = 4e-27,   Method: Compositional matrix adjust.
 Identities = 51/77 (66%), Positives = 65/77 (84%)

Query: 1   MEDFEELIRELKKRGMHLMTDIVVNHSSTEHPWFQEARKSKDNPYRNYYIWRDPAPDGGV 60
           M+DF+ L+ E+ +RGM L+ D+VVNH+STEH WF++AR SKDNPYR++YIWRDP PDG  
Sbjct: 79  MDDFDRLLEEVHRRGMKLIMDMVVNHTSTEHEWFKQARTSKDNPYRDFYIWRDPKPDGSA 138

Query: 61  PNNWQSKFGGPAWQFDE 77
           P NWQSKFGG AW++DE
Sbjct: 139 PTNWQSKFGGSAWEYDE 155


>C3IZJ9.1 C3IZJ9_9BACI Alpha,alpha-phosphotrehalase
          Length = 563

 Score =  121 bits (303), Expect = 2e-26,   Method: Compositional matrix adjust.
 Identities = 51/77 (66%), Positives = 64/77 (83%)

Query: 1   MEDFEELIRELKKRGMHLMTDIVVNHSSTEHPWFQEARKSKDNPYRNYYIWRDPAPDGGV 60
           M DF+ L+ E+  RGM L+ D+VVNH+ST+H WF++AR SK NPYR++YIWRDP PDGG 
Sbjct: 79  MGDFDRLLHEVHARGMKLVMDMVVNHTSTDHEWFKQARSSKTNPYRSFYIWRDPKPDGGA 138

Query: 61  PNNWQSKFGGPAWQFDE 77
           PNNWQSKFGG AW++DE
Sbjct: 139 PNNWQSKFGGSAWEYDE 155


  Database: pfamseq
    Posted date:  Aug 5, 2010  3:22 PM
  Number of letters in database: 3,060,696,203
  Number of sequences in database:  9,421,015
  
Lambda     K      H
   0.318    0.138    0.472 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 9421015
Number of Hits to DB: 652,966,702
Number of extensions: 25048635
Number of successful extensions: 59788
Number of sequences better than 1.0e-05: 2239
Number of HSP's gapped: 57971
Number of HSP's successfully gapped: 2239
Length of query: 89
Length of database: 3,060,696,203
Length adjustment: 59
Effective length of query: 30
Effective length of database: 2,504,856,318
Effective search space: 75145689540
Effective search space used: 75145689540
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 125 (52.8 bits)