BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0034.p (375 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKV8.1 B3KKV8_9BACI Biotin--acetyl-CoA-carboxylase ligase EC=6... 224 1e-56 B1DM04.1 B1DM04_9BACL Biotin--acetyl-CoA-carboxylase ligase EC=6... 196 2e-48 A6CRW9.1 A6CRW9_9BACI BirA 177 2e-42 C3IYW6.1 C3IYW6_9BACI Bifunctional BirA, biotin operon repressor... 176 3e-42 B7Y7N2.1 B7Y7N2_9BACI Bifunctional BirA, biotin operon repressor... 176 3e-42 >B3KKV8.1 B3KKV8_9BACI Biotin--acetyl-CoA-carboxylase ligase EC=6.3.4.15 Length = 329 Score = 224 bits (570), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 120/260 (46%), Positives = 160/260 (61%), Gaps = 8/260 (3%) Query: 118 RLAGGRDTFVPGWLDR-----VQLLETVVSTQEEAKKLAESGAPQGTTVRAEEQTGGRGR 172 RL+G D G R ++LL+ STQE+A++LAE GA +GT V +EEQTGGRGR Sbjct: 68 RLSG--DQLTAGMTSRYFGKSIKLLDKTTSTQEDARQLAEEGAAEGTLVISEEQTGGRGR 125 Query: 173 MGRKWHSPRGKGIWMSVVLRPEXXXXXXXXXXXXAGVAVCLAIREVTGVQAGIKWPNDLL 232 MGRK+HSPRGKGIWMS+VLRP+ GVAVC AI + GVQ IKWPND+L Sbjct: 126 MGRKFHSPRGKGIWMSLVLRPKQPLHLTQQLTLLTGVAVCRAIAKSAGVQTDIKWPNDIL 185 Query: 233 AGGRKICGILLESSLREGRLHYAIAGIGIAANLTEEDYPPDLQGIGTSLLIEGGGIPVDR 292 G+K+CGILLES+ + R+ Y IAGIGI+ANL E D+P +L+ + TS+ I GG V+R Sbjct: 186 YQGKKVCGILLESATEDERVRYCIAGIGISANLKEADFPEELRNVATSIRI-AGGKTVNR 244 Query: 293 SALTAAVLTXXXXXXXXXXXQGFAPIRELWESMSVTLGRQVGFNTPQGRCEGTAVXXXXX 352 ++L ++++ QGF P+ LWE++S T+GR+V T + R G A Sbjct: 245 TSLIQSIMSEMEVLYELYNEQGFEPVASLWEALSGTVGREVQVQTARDRFSGIATGLNQD 304 Query: 353 XXXXXXXXTGSVVSVLSGEI 372 G +V V SG+I Sbjct: 305 GALLVRNQHGELVPVYSGDI 324 >B1DM04.1 B1DM04_9BACL Biotin--acetyl-CoA-carboxylase ligase EC=6.3.4.15 Length = 326 Score = 196 bits (499), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 109/235 (46%), Positives = 134/235 (57%) Query: 138 ETVVSTQEEAKKLAESGAPQGTTVRAEEQTGGRGRMGRKWHSPRGKGIWMSVVLRPEXXX 197 E V STQ A+KLAE GAP+GT V AE+Q GRGRMGR W SP+GKGIWMS+VLRP Sbjct: 87 EAVDSTQNLAQKLAEEGAPEGTLVIAEQQHNGRGRMGRGWVSPQGKGIWMSMVLRPSVPI 146 Query: 198 XXXXXXXXXAGVAVCLAIREVTGVQAGIKWPNDLLAGGRKICGILLESSLREGRLHYAIA 257 VA+C ++R +T + GIKWPNDLL G+KI GILLES+ E RL Y IA Sbjct: 147 HFAPQLTLLTAVALCRSLRRITSLPIGIKWPNDLLIEGKKISGILLESTAEEERLRYVIA 206 Query: 258 GIGIAANLTEEDYPPDLQGIGTSLLIEGGGIPVDRSALTAAVLTXXXXXXXXXXXQGFAP 317 GIGI+ NL + DYP ++ TSL I GG DR + L +GFAP Sbjct: 207 GIGISVNLEKTDYPEEMLEKVTSLRIANGGDGFDRITVITDFLQEWEQLYNLYLDKGFAP 266 Query: 318 IRELWESMSVTLGRQVGFNTPQGRCEGTAVXXXXXXXXXXXXXTGSVVSVLSGEI 372 I LWE++SV+L + TPQG +G + GS V V S E+ Sbjct: 267 IITLWEALSVSLNKPARLITPQGTIDGVPIGLDASGALTVRKDDGSTVVVFSAEM 321 >A6CRW9.1 A6CRW9_9BACI BirA Length = 317 Score = 177 bits (449), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 97/241 (40%), Positives = 133/241 (55%), Gaps = 1/241 (0%) Query: 134 VQLLETVVSTQEEAKKLAESGAPQGTTVRAEEQTGGRGRMGRKWHSPRGKGIWMSVVLRP 193 ++ +E+V STQ+ A LA+ GA +GTTV A+EQT GRGR+ R WHSP+G GIWMS++L+P Sbjct: 77 IKYIESVPSTQKIAHALAQEGAEEGTTVIADEQTEGRGRLLRSWHSPKGTGIWMSIILKP 136 Query: 194 EXXXXXXXXXXXXAGVAVCLAIREVTGVQAGIKWPNDLLAGGRKICGILLESSLREGRLH 253 + A VAV AI EV G+ IKWPND+L GRK+ GIL E +++ Sbjct: 137 QLPPQRAPQFTLIAAVAVVQAIEEVCGLSPEIKWPNDILLNGRKLTGILTELQAEADKIN 196 Query: 254 YAIAGIGIAANLTEEDYPPDLQGIGTSLLIEGGGIPVDRSALTAAVLTXXXXXXXXXXXQ 313 I GIG+ N ED+P +LQ + TSL +E V R L V+ + Sbjct: 197 SIIIGIGMNVNQKAEDFPEELQSVATSLFMEKQE-KVSRVKLIQRVMEKLEIYYDLYMEK 255 Query: 314 GFAPIRELWESMSVTLGRQVGFNTPQGRCEGTAVXXXXXXXXXXXXXTGSVVSVLSGEIE 373 GF PI+ LWES ++++GRQ+ T G G A+ G V V S +IE Sbjct: 256 GFTPIKLLWESYAISIGRQITARTITGSIHGKALGINEDGVLRIEDAEGQVHEVYSADIE 315 Query: 374 M 374 + Sbjct: 316 L 316 >C3IYW6.1 C3IYW6_9BACI Bifunctional BirA, biotin operon repressor/biotin--acetyl-CoA-carboxylase ligase EC=6.3.4.15 Length = 329 Score = 176 bits (446), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 98/213 (46%), Positives = 120/213 (56%), Gaps = 1/213 (0%) Query: 134 VQLLETVVSTQEEAKKLAESGAPQGTTVRAEEQTGGRGRMGRKWHSPRGKGIWMSVVLRP 193 + + V STQ A +LA GAP+GT V AEEQ GRGR+ RKW SP+G GIWMS++LRP Sbjct: 90 IHFFDEVDSTQRIAARLAYEGAPEGTLVVAEEQKAGRGRLDRKWFSPKGTGIWMSLILRP 149 Query: 194 EXXXXXXXXXXXXAGVAVCLAIREVTGVQAGIKWPNDLLAGGRKICGILLESSLREGRLH 253 A VAV AI+EVTG+ IKWPND+L GRK GIL E R+H Sbjct: 150 AIPPQRAPQLTLLAAVAVSQAIQEVTGLVPDIKWPNDILLDGRKCVGILTELQADPDRVH 209 Query: 254 YAIAGIGIAANLTEEDYPPDLQGIGTSLLIEGGGIPVDRSALTAAVLTXXXXXXXXXXXQ 313 I GIGI N T E +P D++ I TSL IE GG PV R+ L +L Sbjct: 210 SVIIGIGINVNQTIEQFPEDIRAIATSLAIEKGG-PVKRAPLIQEILFRLERLYEQYLAH 268 Query: 314 GFAPIRELWESMSVTLGRQVGFNTPQGRCEGTA 346 GF PI+ LWE +V++G+ V T G G A Sbjct: 269 GFRPIKLLWEGYAVSIGKPVTARTLNGVIHGIA 301 >B7Y7N2.1 B7Y7N2_9BACI Bifunctional BirA, biotin operon repressor/biotin--acetyl-CoA-carboxylase ligase EC=6.3.4.15 Length = 329 Score = 176 bits (446), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 98/213 (46%), Positives = 120/213 (56%), Gaps = 1/213 (0%) Query: 134 VQLLETVVSTQEEAKKLAESGAPQGTTVRAEEQTGGRGRMGRKWHSPRGKGIWMSVVLRP 193 + + V STQ A +LA GAP+GT V AEEQ GRGR+ RKW SP+G GIWMS++LRP Sbjct: 90 IHFFDEVDSTQRIAARLAYEGAPEGTLVVAEEQKAGRGRLDRKWFSPKGTGIWMSLILRP 149 Query: 194 EXXXXXXXXXXXXAGVAVCLAIREVTGVQAGIKWPNDLLAGGRKICGILLESSLREGRLH 253 A VAV AI+EVTG+ IKWPND+L GRK GIL E R+H Sbjct: 150 AIPPQRAPQLTLLAAVAVSQAIQEVTGLVPDIKWPNDILLDGRKCVGILTELQADPDRVH 209 Query: 254 YAIAGIGIAANLTEEDYPPDLQGIGTSLLIEGGGIPVDRSALTAAVLTXXXXXXXXXXXQ 313 I GIGI N T E +P D++ I TSL IE GG PV R+ L +L Sbjct: 210 SVIIGIGINVNQTIEQFPEDIRAIATSLAIEKGG-PVKRAPLIQEILFRLERLYEQYLAH 268 Query: 314 GFAPIRELWESMSVTLGRQVGFNTPQGRCEGTA 346 GF PI+ LWE +V++G+ V T G G A Sbjct: 269 GFRPIKLLWEGYAVSIGKPVTARTLNGVIHGIA 301 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.318 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,090,113,007 Number of extensions: 81542155 Number of successful extensions: 182346 Number of sequences better than 1.0e-05: 1345 Number of HSP's gapped: 180925 Number of HSP's successfully gapped: 1345 Length of query: 375 Length of database: 3,060,696,203 Length adjustment: 137 Effective length of query: 238 Effective length of database: 1,770,017,148 Effective search space: 421264081224 Effective search space used: 421264081224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)