BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0033.p (288 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKV9.1 B3KKV9_9BACI 3-methyl-2-oxobutanoate hydroxymethyltrans... 444 e-123 B1DM03.1 B1DM03_9BACL 3-methyl-2-oxobutanoate hydroxymethyltrans... 379 e-103 C1PF21.1 C1PF21_BACCO 3-methyl-2-oxobutanoate hydroxymethyltrans... 318 5e-85 C0ZCH0.1 C0ZCH0_BREBN 3-methyl-2-oxobutanoate hydroxymethyltrans... 316 2e-84 A4J0V6.1 A4J0V6_DESRM 3-methyl-2-oxobutanoate hydroxymethyltrans... 308 4e-82 >B3KKV9.1 B3KKV9_9BACI 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Length = 295 Score = 444 bits (1141), Expect = e-123, Method: Compositional matrix adjust. Identities = 213/286 (74%), Positives = 242/286 (84%), Gaps = 4/286 (1%) Query: 1 MADKQALNIVKMKKMKADGVPLSMLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTT 60 MA KQ LNIVK+K MK GVP++MLTAYDYPSA LAEEA ID+ILVGDSLGNVVLGY++T Sbjct: 1 MAGKQPLNIVKLKNMKKKGVPITMLTAYDYPSAKLAEEADIDMILVGDSLGNVVLGYNST 60 Query: 61 LPVTIEDMVYHTRSVTRGARNTFIVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEG 120 +PVT++DMVYHTR+V RGA +TFIVADMPFMTYHGGIDE+LR VRRLMQEG AHAVKMEG Sbjct: 61 IPVTLDDMVYHTRAVARGAEDTFIVADMPFMTYHGGIDESLRGVRRLMQEGHAHAVKMEG 120 Query: 121 GLEICSTVSAVVSAGVPVLGHIGLTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGV 180 G EI TV +V +GVPVLGHIGLTPQSVN IGGYRIQGKDA+DAQRLM++AKALEAAG Sbjct: 121 GEEIADTVKRIVQSGVPVLGHIGLTPQSVNQIGGYRIQGKDAQDAQRLMNDAKALEAAGA 180 Query: 181 FGIXXXXXXXXXXXXISKAVSIPTIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYA 240 F I ISKA+SIPTIGIGAGR+CDGQVLVFHD+L+YASPYR+KRFVKTYA Sbjct: 181 FAIVLELVTEEAAADISKALSIPTIGIGAGRYCDGQVLVFHDILKYASPYRDKRFVKTYA 240 Query: 241 DVGTLIREGISSYVQEVKDRSFPAESHVF----NADEHVLDSLYGA 282 DVG++IREGI+ YV+EVKDR+FPAE HVF NA +LYG+ Sbjct: 241 DVGSVIREGITQYVKEVKDRAFPAEEHVFKTENNAGSETTTALYGS 286 >B1DM03.1 B1DM03_9BACL 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Length = 306 Score = 379 bits (972), Expect = e-103, Method: Compositional matrix adjust. Identities = 180/279 (64%), Positives = 220/279 (78%) Query: 4 KQALNIVKMKKMKADGVPLSMLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTTLPV 63 ++ L I +KKMK D P+SMLTAYDYPSA+LAEEAG+D+ILVGDSLGNVVLGYDTT+PV Sbjct: 2 RKRLTITNLKKMKQDKNPISMLTAYDYPSAVLAEEAGLDVILVGDSLGNVVLGYDTTIPV 61 Query: 64 TIEDMVYHTRSVTRGARNTFIVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEGGLE 123 T++DM+YHTR+V RGA+NTFIV DMPFMTY G + TLR+ RLMQEG A A+K+EGG E Sbjct: 62 TLDDMIYHTRAVARGAKNTFIVTDMPFMTYGIGRETTLRNAARLMQEGGAQALKLEGGTE 121 Query: 124 ICSTVSAVVSAGVPVLGHIGLTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGVFGI 183 I S V A+V AG+PV+GHIGLTPQSV+ +GGY++QGK +A++LM++AKALEAAG F I Sbjct: 122 IASEVEALVKAGIPVMGHIGLTPQSVHQLGGYKVQGKLDAEAEKLMEDAKALEAAGAFAI 181 Query: 184 XXXXXXXXXXXXISKAVSIPTIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYADVG 243 ISK + IPTIGIGAGR CDGQVLV+HD+L+Y+SPY EK+ VKTYAD+G Sbjct: 182 VLELVTEPLAAAISKELKIPTIGIGAGRGCDGQVLVYHDILQYSSPYIEKKMVKTYADIG 241 Query: 244 TLIREGISSYVQEVKDRSFPAESHVFNADEHVLDSLYGA 282 + IR ISSYV EVK +FPAE H F A ++ LYGA Sbjct: 242 STIRNAISSYVNEVKAGAFPAEEHSFGAKPAAINHLYGA 280 >C1PF21.1 C1PF21_BACCO 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Length = 279 Score = 318 bits (814), Expect = 5e-85, Method: Compositional matrix adjust. Identities = 152/282 (53%), Positives = 205/282 (72%), Gaps = 4/282 (1%) Query: 4 KQALNIVKMKKMKADGVPLSMLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTTLPV 63 KQ + VKMK A ++M+TAYDYPSA LAEEAG+D+ILVGDSLG VVLGYD+T+PV Sbjct: 2 KQTTDFVKMK---AQEEKIAMVTAYDYPSAKLAEEAGVDMILVGDSLGMVVLGYDSTIPV 58 Query: 64 TIEDMVYHTRSVTRGARNTFIVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEGGLE 123 T+ DMV+HT++V RGA+NTFIV DMPFM+YH +ETL++ R++Q+G AHAVK+EGG E Sbjct: 59 TVADMVHHTKAVKRGAKNTFIVTDMPFMSYHLSREETLKNGARIIQDGGAHAVKLEGGKE 118 Query: 124 ICSTVSAVVSAGVPVLGHIGLTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGVFGI 183 + + + A+ +G+PV+ HIGLTPQ V + GGY++QGK A+ A++L+++AKA E AG F + Sbjct: 119 VAAQIEALTESGIPVMAHIGLTPQKVGIFGGYKVQGKTAEAAKKLLEDAKACEEAGAFAV 178 Query: 184 XXXXXXXXXXXXISKAVSIPTIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYADVG 243 ++ A+SIPTIGIGAG DGQVLVFHDL++Y S + +FVK +AD Sbjct: 179 VLECVPRQLSEEVTAALSIPTIGIGAGLDVDGQVLVFHDLVKYGSE-KVAKFVKQFADAD 237 Query: 244 TLIREGISSYVQEVKDRSFPAESHVFNADEHVLDSLYGAAGK 285 ++R G+ YVQEVK+R+FP E H + E L +LYG K Sbjct: 238 AVMRAGLRQYVQEVKNRTFPEEKHTYTMKEEELATLYGGHAK 279 >C0ZCH0.1 C0ZCH0_BREBN 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Length = 287 Score = 316 bits (809), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 146/279 (52%), Positives = 204/279 (73%), Gaps = 1/279 (0%) Query: 3 DKQALNIVKMKKMKADGVPLSMLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTTLP 62 + + + ++K K P++M+TAYDYP+A L +EAG+D+ILVGDSLG VVLGYD+T+P Sbjct: 4 NDRPITTSSIRKKKETRTPITMVTAYDYPAAKLVDEAGVDMILVGDSLGMVVLGYDSTIP 63 Query: 63 VTIEDMVYHTRSVTRGARNTFIVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEGGL 122 VT+EDM++HT++VTRGA+ F+V D+PF++YHG ++E +++ RLMQEG A AVKMEGG Sbjct: 64 VTMEDMLHHTKAVTRGAKRAFVVTDLPFLSYHGTVEEAVKNAGRLMQEGLAKAVKMEGGR 123 Query: 123 EICSTVSAVVSAGVPVLGHIGLTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGVFG 182 E+ ++ V AG+PV+GHIGLTPQSV+ +GGY++QG+D + A++L+DEA A++ AG F Sbjct: 124 ELAPIITRCVQAGIPVVGHIGLTPQSVHQLGGYKVQGRDLEAAKKLLDEALAIQEAGAFA 183 Query: 183 IXXXXXXXXXXXXISKAVSIPTIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYADV 242 I I+ + IP IGIGAG CDGQVLVFHD++ YAS K FVK YA++ Sbjct: 184 IVLECVPEEVAGMIADKLDIPVIGIGAGATCDGQVLVFHDMVGYASDITPK-FVKRYANI 242 Query: 243 GTLIREGISSYVQEVKDRSFPAESHVFNADEHVLDSLYG 281 G IRE + +Y +EV+ RSFP + HVF+A E + LYG Sbjct: 243 GETIREAVETYNKEVEARSFPGQEHVFHASEETIKELYG 281 >A4J0V6.1 A4J0V6_DESRM 3-methyl-2-oxobutanoate hydroxymethyltransferase EC=2.1.2.11 Length = 277 Score = 308 bits (789), Expect = 4e-82, Method: Compositional matrix adjust. Identities = 145/269 (53%), Positives = 198/269 (73%), Gaps = 1/269 (0%) Query: 12 MKKMKADGVPLSMLTAYDYPSALLAEEAGIDLILVGDSLGNVVLGYDTTLPVTIEDMVYH 71 ++MK + P++MLTAYDYP+A+L + +GID ILVGDSLGNVVLGYD+T+PVT++DM++H Sbjct: 10 FRRMKQEAKPIAMLTAYDYPTAMLVDASGIDGILVGDSLGNVVLGYDSTVPVTMDDMIHH 69 Query: 72 TRSVTRGARNTFIVADMPFMTYHGGIDETLRSVRRLMQEGRAHAVKMEGGLEICSTVSAV 131 R+VTRGA+ +V DMPF++YH +E++R+ RLMQEG A AVK+EGG E+ TV A+ Sbjct: 70 LRAVTRGAKRALVVGDMPFLSYHISREESVRNAGRLMQEGLAQAVKLEGGREVADTVKAI 129 Query: 132 VSAGVPVLGHIGLTPQSVNMIGGYRIQGKDAKDAQRLMDEAKALEAAGVFGIXXXXXXXX 191 AG+PV+GH+GLTPQSV+ +GGY++QGKD + A+RL+++AKALEAAGVF I Sbjct: 130 TEAGIPVMGHLGLTPQSVHQMGGYKVQGKDGEAARRLLEDAKALEAAGVFAIVLECIPQQ 189 Query: 192 XXXXISKAVSIPTIGIGAGRFCDGQVLVFHDLLRYASPYREKRFVKTYADVGTLIREGIS 251 +++AV +PTIGIGAG CDGQ+LV HDLL S + K FVK YA++ I + Sbjct: 190 LAKVVTEAVKVPTIGIGAGSDCDGQILVTHDLLGMFSDFTPK-FVKKYANLKEQIIKACE 248 Query: 252 SYVQEVKDRSFPAESHVFNADEHVLDSLY 280 Y QEV+D++FP HVFN + L +Y Sbjct: 249 DYQQEVRDKAFPGPGHVFNMADEELKKIY 277 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,600,376,914 Number of extensions: 62936489 Number of successful extensions: 154347 Number of sequences better than 1.0e-05: 1336 Number of HSP's gapped: 151730 Number of HSP's successfully gapped: 1336 Length of query: 288 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 154 Effective length of database: 1,798,280,193 Effective search space: 276935149722 Effective search space used: 276935149722 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)