BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0027.p (433 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKW5.1 B3KKW5_9BACI Amidohydrolase 667 0.0 B1DLA2.1 B1DLA2_9BACL Amidohydrolase 588 e-166 C0ZCH7.1 C0ZCH7_BREBN Putative uncharacterized protein 479 e-133 C0GHU5.1 C0GHU5_9FIRM Amidohydrolase 425 e-117 B5YDN9.1 MTAD_DICT6 5-methylthioadenosine/S-adenosylhomocysteine... 415 e-114 >B3KKW5.1 B3KKW5_9BACI Amidohydrolase Length = 433 Score = 667 bits (1721), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/430 (75%), Positives = 368/430 (85%) Query: 1 MSSNKTVIVNGRFLVPGTAQPVLSGYMTIEKDLITYIGEVKPVIEEGTQTIDGSRLLFMP 60 M++ K ++ NG F V + VL GYM +E D ITY+GE +PV+EEGT+ IDG+ L F+P Sbjct: 1 MTTTKWMVKNGTFAVLEEDRSVLRGYMVVENDRITYLGEEEPVVEEGTEIIDGTHLFFLP 60 Query: 61 GLVNTHGHTAMSLLRGYGDDMVLQNWLQEKMWPMEEKFTGDDVYWGTALSVLEMLKGGTT 120 GLVNTHGH AMSLLRGYGDD+ LQ WLQEKMWPME KFT DDVYWGT+LSVLEM+KGGTT Sbjct: 61 GLVNTHGHAAMSLLRGYGDDLALQVWLQEKMWPMEAKFTSDDVYWGTSLSVLEMIKGGTT 120 Query: 121 TFLDMYDHMDRVAEVTEQSGIRAVLMRGVIGLCPEDVQNHKLAEAVAFARNWHGKADGRI 180 TF+DMYDHMD+VA+V + SG+R VL RGVIGLCP +VQ KL EAVAFA++WHGKADGRI Sbjct: 121 TFVDMYDHMDQVAKVVQDSGMRGVLTRGVIGLCPPEVQQQKLDEAVAFAKDWHGKADGRI 180 Query: 181 TTMISPHAPYTCPPEFFVKFVQAAHDLDLPMHTHMSETRREVEQNAADYGLRPVAHLEKL 240 TTMISPHAPYTCPP+F KFVQA+HDL+LP+HTHMSET EV QN DYGLRPVAHLEKL Sbjct: 181 TTMISPHAPYTCPPDFIEKFVQASHDLNLPLHTHMSETETEVAQNVKDYGLRPVAHLEKL 240 Query: 241 GMFTRPSLVAHGVHLNDEEIEILARYHVGVSHNPGSNLKLASGVARVPELLRAGVTVSLG 300 GMF+RP+L+AH VHL DEEIE+LA++ V VSHNPGSNLKLASGVARVP LL+AG+TVSLG Sbjct: 241 GMFSRPTLLAHAVHLTDEEIEVLAKHQVAVSHNPGSNLKLASGVARVPALLKAGITVSLG 300 Query: 301 TDGAASNNNLDMFEEMRLAALIHKGVSGDPTAVPAQEALLMATEYGAQSIFLEGVGRLAP 360 TDG ASNNNLDMFEEMRLAALIHKGVSGDPTA+PA EAL M TEYGA+S FLE +G LA Sbjct: 301 TDGPASNNNLDMFEEMRLAALIHKGVSGDPTAIPASEALRMGTEYGAKSAFLEDIGMLAV 360 Query: 361 GMKADFIALDIDQPHFLPHTDLLSHAVYSASAKDVAHVWVDGKQVVKHGQCLTLDEEQIR 420 GMKAD IAL+ DQ HFLP TD +SHA+YSASAKDV HVWVDGKQVVK+G LTLDEE+IR Sbjct: 361 GMKADMIALNTDQAHFLPRTDYISHAIYSASAKDVEHVWVDGKQVVKNGASLTLDEERIR 420 Query: 421 RKAQETFEGL 430 R+AQ FE L Sbjct: 421 REAQRAFERL 430 >B1DLA2.1 B1DLA2_9BACL Amidohydrolase Length = 409 Score = 588 bits (1516), Expect = e-166, Method: Compositional matrix adjust. Identities = 276/408 (67%), Positives = 333/408 (81%), Gaps = 2/408 (0%) Query: 27 MTIEKDLITYIGEVKPV-IEEGTQTIDGSRLLFMPGLVNTHGHTAMSLLRGYGDDMVLQN 85 M + + ITYIG P ++E + +DGS+L FMPGL+NTHGH AMSLLRGY DD LQ Sbjct: 1 MVVTDNQITYIGAEAPTGLDERAERLDGSKLAFMPGLINTHGHAAMSLLRGYSDDQNLQV 60 Query: 86 WLQEKMWPMEEKFTGDDVYWGTALSVLEMLKGGTTTFLDMYDHMDRVAEVTEQSGIRAVL 145 WL++KMWPME K+ D G+AL+++EMLK GTT F+DMYD MD+VA++ EQSGIR+ L Sbjct: 61 WLEQKMWPMEAKYVDQDTRAGSALAIVEMLKTGTTAFVDMYDRMDQVAQMVEQSGIRSAL 120 Query: 146 MRGVIGLCPEDVQNHKLAEAVAFARNWHGKADGRITTMISPHAPYTCPPEFFVKFVQAAH 205 RGVIGLC E++Q KL EA+AFAR+W+GKADGRITTMISPHAPYTCPP++ KFVQAAH Sbjct: 121 TRGVIGLCSEEIQQAKLKEAIAFARDWNGKADGRITTMISPHAPYTCPPDYIEKFVQAAH 180 Query: 206 DLDLPMHTHMSETRREVEQNAADYGLRPVAHLEKLGMFTRPSLVAHGVHLNDEEIEILAR 265 D DLP+HTHMSET EVEQN DYG RPV HL+KLG F+RP+LVAH VHLNDEEI +LA Sbjct: 181 DYDLPVHTHMSETLAEVEQNVRDYGSRPVEHLDKLGFFSRPALVAHAVHLNDEEIALLAE 240 Query: 266 YHVGVSHNPGSNLKLASGVARVPELLRAGVTVSLGTDGAASNNNLDMFEEMRLAALIHKG 325 +V VSHNP SNLKLASGVARVPELLRAGVTVSLGTD ASNNNLD+F+E++ AAL+HKG Sbjct: 241 RNVAVSHNPVSNLKLASGVARVPELLRAGVTVSLGTDSVASNNNLDLFKEIKFAALLHKG 300 Query: 326 VSGDPTAVPAQEALLMATEYGAQSIFLEG-VGRLAPGMKADFIALDIDQPHFLPHTDLLS 384 +SGDPT +PA EAL M T YGA+SI+ EG +G+LA GMKADFIA+D+DQPH+ P TD++S Sbjct: 301 ISGDPTVIPAMEALRMGTVYGARSIWQEGSIGQLATGMKADFIAIDLDQPHYYPLTDIVS 360 Query: 385 HAVYSASAKDVAHVWVDGKQVVKHGQCLTLDEEQIRRKAQETFEGLLK 432 H VYS S +DV HVWVDGK+VV +G+C +DEE+IR +AQ +FE LL Sbjct: 361 HLVYSGSGRDVKHVWVDGKKVVHNGECTMMDEEKIRFEAQASFERLLN 408 >C0ZCH7.1 C0ZCH7_BREBN Putative uncharacterized protein Length = 434 Score = 479 bits (1232), Expect = e-133, Method: Compositional matrix adjust. Identities = 233/432 (53%), Positives = 310/432 (71%), Gaps = 4/432 (0%) Query: 5 KTVIVNGRFL-VPGTAQPVLSGYMTIEKDLITYIGEVKPVIEEG--TQTIDGSRLLFMPG 61 KT++++ + V T + + G + E + ITY+G + E + ID +PG Sbjct: 3 KTILIHATVITVNDTNEVIHDGAVAFEGNKITYVGPTPEDLSEAGYDEVIDQKGDYILPG 62 Query: 62 LVNTHGHTAMSLLRGYGDDMVLQNWLQEKMWPMEEKFTGDDVYWGTALSVLEMLKGGTTT 121 L+NTHGH MSLLRGY DD+ LQ WL++KMWP+E +FTGD V WGT LS++EM++ GTTT Sbjct: 63 LINTHGHAGMSLLRGYADDLPLQQWLEDKMWPLEAQFTGDTVKWGTQLSLIEMIRTGTTT 122 Query: 122 FLDMYDHMDRVAEVTEQSGIRAVLMRGVIGLCPEDVQNHKLAEAVAFARNWHGKADGRIT 181 F+DMYDHMD VA+ + +G+RA L RG+IGLC E+ + KL +A FA+ WH +ADGRIT Sbjct: 123 FVDMYDHMDVVAKEVDAAGMRARLCRGMIGLCSEEERQTKLKDATLFAKEWHNQADGRIT 182 Query: 182 TMISPHAPYTCPPEFFVKFVQAAHDLDLPMHTHMSETRREVEQNAADYGLRPVAHLEKLG 241 M++PHAPYTC PEF + ++ A +L LP+H HMSET EV QN DYG RPVAHLEKLG Sbjct: 183 VMMAPHAPYTCSPEFITQIIEKADELSLPLHIHMSETAWEVGQNEKDYGQRPVAHLEKLG 242 Query: 242 MFTRPSLVAHGVHLNDEEIEILARYHVGVSHNPGSNLKLASGVARVPELLRAGVTVSLGT 301 MF RP+LVAH VHL DEEI+ILA+Y+V VSHN SNLKLASGVA VP++L GV+VSLGT Sbjct: 243 MFNRPTLVAHAVHLTDEEIDILAKYNVRVSHNVVSNLKLASGVAPVPKMLAKGVSVSLGT 302 Query: 302 DGAASNNNLDMFEEMRLAALIHKGVSGDPTAVPAQEALLMATEYGAQSIF-LEGVGRLAP 360 D +ASNNNL++FEE++LAA++HKGV+ DP AVPA+EAL MAT YGA+ +F E +G + Sbjct: 303 DSSASNNNLNLFEELKLAAILHKGVNNDPVAVPAEEALRMATRYGAEGVFQQETLGSIEV 362 Query: 361 GMKADFIALDIDQPHFLPHTDLLSHAVYSASAKDVAHVWVDGKQVVKHGQCLTLDEEQIR 420 G +AD I +D Q HF P +SH VY+A+ +DV V GK ++++ + LT+DEE+ Sbjct: 363 GKQADLIVMDSHQAHFHPAHQPISHVVYAANGRDVKDTIVAGKFLMRNHKLLTIDEERAI 422 Query: 421 RKAQETFEGLLK 432 +A F+ L + Sbjct: 423 YEANRVFQTLKR 434 >C0GHU5.1 C0GHU5_9FIRM Amidohydrolase Length = 433 Score = 425 bits (1093), Expect = e-117, Method: Compositional matrix adjust. Identities = 200/399 (50%), Positives = 279/399 (69%), Gaps = 2/399 (0%) Query: 27 MTIEKDLITYIGEVKPVIEEGTQTIDGSRLLFMPGLVNTHGHTAMSLLRGYGDDMVLQNW 86 + ++ ++I+Y+G K +E+ + I+G L PG VN HGH AMSLLR DD+ L W Sbjct: 25 VVVDNNIISYVGPKKEWVEDFAKVINGRGKLVAPGFVNAHGHAAMSLLRSLADDVPLMYW 84 Query: 87 LQEKMWPMEEKFTGDDVYWGTALSVLEMLKGGTTTFLDMYDHMDRVAEVTEQSGIRAVLM 146 L++++WP+E K +DVYWGT L++LEM+KGGTTTF DMY MD+VAE TE++GIRAVL Sbjct: 85 LEKRIWPVEAKLKREDVYWGTMLAILEMIKGGTTTFTDMYFFMDQVAEATEETGIRAVLA 144 Query: 147 RGVIGLCPEDVQNHKLAEAVAFARNWHGKADGRITTMISPHAPYTCPPEFFVKFVQAAHD 206 RG++G+ + L E+ F NW G ADGRI+TM+ PHAPYTCPP++ + + Sbjct: 145 RGLVGIG--HMSEQGLEESQQFVENWQGGADGRISTMLGPHAPYTCPPDYLKRVLALQEK 202 Query: 207 LDLPMHTHMSETRREVEQNAADYGLRPVAHLEKLGMFTRPSLVAHGVHLNDEEIEILARY 266 LD+P+ H+ ETR EV++ ++G+ PV + G+F P + AH VHL ++I+IL + Sbjct: 203 LDVPVQIHLCETRDEVDRIQKEHGVTPVELVRDTGLFQAPVIAAHCVHLTVDDIDILREF 262 Query: 267 HVGVSHNPGSNLKLASGVARVPELLRAGVTVSLGTDGAASNNNLDMFEEMRLAALIHKGV 326 V V+HNPGSNLKL SG++ VP+LL+ G+TV LGTDGAASNNNLDM EEMRLAAL+HKG Sbjct: 263 DVRVAHNPGSNLKLGSGISPVPDLLKRGITVGLGTDGAASNNNLDMMEEMRLAALLHKGS 322 Query: 327 SGDPTAVPAQEALLMATEYGAQSIFLEGVGRLAPGMKADFIALDIDQPHFLPHTDLLSHA 386 DPTA+ A++AL M T AQ++FLE VG + GMKAD I +D+ +PH P DL++H Sbjct: 323 RMDPTAITARQALAMGTRESAQALFLEDVGTIEAGMKADLIMMDLQKPHLTPQHDLVAHL 382 Query: 387 VYSASAKDVAHVWVDGKQVVKHGQCLTLDEEQIRRKAQE 425 VY+A D+ V V+G+ +++ G T+DEE+I +AQ+ Sbjct: 383 VYAAQPSDITLVMVNGRILMEDGNLTTMDEEKILFQAQQ 421 >B5YDN9.1 MTAD_DICT6 5-methylthioadenosine/S-adenosylhomocysteine deaminase EC=3.5.4.n1 EC=3.5.4.28 Length = 426 Score = 415 bits (1066), Expect = e-114, Method: Compositional matrix adjust. Identities = 204/407 (50%), Positives = 275/407 (67%), Gaps = 3/407 (0%) Query: 27 MTIEKDLITYIGEVKPVIEEGTQTIDGSRLLFMPGLVNTHGHTAMSLLRGYGDDMVLQNW 86 + IE D+I I + K E IDG + +PGLVNTH H AM+L RG+ DD+ L+ W Sbjct: 22 ILIENDIIKQISDSKS-FERIDYIIDGKNKIALPGLVNTHTHLAMTLFRGFADDLPLEEW 80 Query: 87 LQEKMWPMEEKFTGDDVYWGTALSVLEMLKGGTTTFLDMYDHMDRVAEVTEQSGIRAVLM 146 L+EK+WP E K T DDVYWG+ L + EM++GGT F DMY MD +A+ +SGI+A L Sbjct: 81 LEEKIWPQEAKLTADDVYWGSLLGICEMIRGGTIAFSDMYFFMDEMAKAVAESGIKASLS 140 Query: 147 RGVIGLCPEDVQNHKLAEAVAFARNWHGKADGRITTMISPHAPYTCPPEFFVKFVQAAHD 206 G+IG+ + +N L V+FA+NWH DGRI M+ PHAPYTCPP F K + A + Sbjct: 141 VGMIGIAGD--ENETLNRGVSFAKNWHNAEDGRIRVMLGPHAPYTCPPSFLEKVIDKAIE 198 Query: 207 LDLPMHTHMSETRREVEQNAADYGLRPVAHLEKLGMFTRPSLVAHGVHLNDEEIEILARY 266 + L +HTH+SET EVE YGL P+ ++++G+F P L AH V ++DEEIEIL+ Sbjct: 199 MSLGIHTHLSETYLEVENIKNMYGLTPIRLMDRVGLFNVPVLAAHCVFVDDEEIEILSEK 258 Query: 267 HVGVSHNPGSNLKLASGVARVPELLRAGVTVSLGTDGAASNNNLDMFEEMRLAALIHKGV 326 VGV+HNP SNLKLASGVA V ++L GV V LGTDG ASNNNLDM+EE+RL A +HKGV Sbjct: 259 GVGVAHNPQSNLKLASGVAPVKKMLEKGVKVGLGTDGPASNNNLDMWEEIRLVATLHKGV 318 Query: 327 SGDPTAVPAQEALLMATEYGAQSIFLEGVGRLAPGMKADFIALDIDQPHFLPHTDLLSHA 386 DP VPA++AL+MAT+ G + + E G + G KAD I +D+++PHF P +L+SH Sbjct: 319 ERDPICVPARDALIMATKNGMEILGFENSGIIKEGYKADLILVDVNKPHFYPRHNLVSHL 378 Query: 387 VYSASAKDVAHVWVDGKQVVKHGQCLTLDEEQIRRKAQETFEGLLKR 433 VYSAS+ DV V VDG+ +++ + TLDEE+I +A++ L+K+ Sbjct: 379 VYSASSSDVDTVIVDGRILMEKRELKTLDEEKIMYEAEKRAFELIKK 425 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,645,828,553 Number of extensions: 112022809 Number of successful extensions: 289336 Number of sequences better than 1.0e-05: 1928 Number of HSP's gapped: 285615 Number of HSP's successfully gapped: 1935 Length of query: 433 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 294 Effective length of database: 1,751,175,118 Effective search space: 514845484692 Effective search space used: 514845484692 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)