BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0023.p (500 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKW8.1 B3KKW8_9BACI Vesicle-fusing ATPase EC=3.6.4.6 855 0.0 B1DL99.1 B1DL99_9BACL AAA ATPase central domain protein 687 0.0 C0ZCI2.1 C0ZCI2_BREBN Probable ATP-dependent zinc metallopeptida... 607 e-172 A4J5U1.1 A4J5U1_DESRM Vesicle-fusing ATPase EC=3.6.4.6 511 e-143 B7DPS6.1 B7DPS6_9BACL Microtubule-severing ATPase EC=3.6.4.3 507 e-142 >B3KKW8.1 B3KKW8_9BACI Vesicle-fusing ATPase EC=3.6.4.6 Length = 500 Score = 855 bits (2209), Expect = 0.0, Method: Compositional matrix adjust. Identities = 406/500 (81%), Positives = 459/500 (91%) Query: 1 MPKFWKEVLIGFVPVLVIFMAFVGINIFPVIIAAGMVGALLLIAHLRGGLTVNAGGDKKR 60 MPK KE+++GF+PVL++F+ FVG+NI P++IAAG+VG LL IA ++GG+ V AG ++KR Sbjct: 1 MPKLAKEIMLGFIPVLLVFLVFVGVNIVPLLIAAGLVGGLLFIAKMKGGIAVGAGNERKR 60 Query: 61 KKNGPSKLTFEEIGGQDNAKQELREALDFLIRHEEISKFGIRPLKGILLTGPPGTGKTLM 120 KK+GP+KLTFEEIGGQDNAKQELREALDFLIRHE+I KFGIRPLKGILLTGPPGTGKTLM Sbjct: 61 KKSGPAKLTFEEIGGQDNAKQELREALDFLIRHEDIKKFGIRPLKGILLTGPPGTGKTLM 120 Query: 121 AKAAAHYTNSVFVAASGSEFVEMYVGVGAGRVRDLFKDARARALKENKQSAIIFIDEIDV 180 AKAAAHYT+S+FVAASGSEFVEMYVGVGAGR+RDLFKDAR RA KENKQ+AIIFIDEIDV Sbjct: 121 AKAAAHYTDSIFVAASGSEFVEMYVGVGAGRIRDLFKDARNRAAKENKQNAIIFIDEIDV 180 Query: 181 IGGKREGGQQREYDQTLNQLLTEMDGIYNNDTPRILLVAATNRKEMLDSALLRPGRFDRH 240 IGGKREGGQQREYDQTLNQLLTEMDGIY+ DTPRIL++AATNRKEMLDSALLRPGRFDRH Sbjct: 181 IGGKREGGQQREYDQTLNQLLTEMDGIYSQDTPRILVIAATNRKEMLDSALLRPGRFDRH 240 Query: 241 IQVDMPDKKGRRSILDLHAQNKPLHEEVDLDKIAEEAYGFSGAQLESVMNEAAIYMMREN 300 IQVD+PDKKGR IL+LHA NKPLH++VDL+KIAEEAYGFSGAQLESVMNEAAIY MREN Sbjct: 241 IQVDLPDKKGRTHILNLHAGNKPLHQDVDLEKIAEEAYGFSGAQLESVMNEAAIYTMREN 300 Query: 301 LTQVEQRHLSLAIDKVMMGEKTDRETNHEEKKRVAIHELGHAIMAELLRPGSVSQVTLTP 360 + QRHLS+AIDKVMMGE++DRE+N EEKKRVAIHELGHAIMAEL+RPGSV QV L+P Sbjct: 301 EQFIHQRHLSMAIDKVMMGERSDRESNLEEKKRVAIHELGHAIMAELVRPGSVKQVALSP 360 Query: 361 RGQALGYVRHNPQTEQYLYTKDYLENQIMIALGGAVAEEMYYGGRSTGSRGDFDQALNIV 420 RG+ALGYVRHNPQ EQYLYTK +LE QIMIALGGA AEE+YYGGRSTGS DF+Q+LNIV Sbjct: 361 RGKALGYVRHNPQQEQYLYTKTFLEEQIMIALGGAAAEEIYYGGRSTGSSNDFEQSLNIV 420 Query: 421 ETMMKSGLTSLGIANLQMVTTEELMKENSKILDELMVRTHELLGKQRNVFDYSLDILMKE 480 ETMMKSGLTSLGI N+ MVTTEELMKEN+ IL+ELM RT LL ++R +FD SLDIL+KE Sbjct: 421 ETMMKSGLTSLGIVNMNMVTTEELMKENNVILEELMNRTKTLLEQKRPIFDNSLDILLKE 480 Query: 481 EVLSGEQFRCQFRDSVLLPA 500 E+LSGEQFRCQFR++ LLPA Sbjct: 481 EILSGEQFRCQFRENALLPA 500 >B1DL99.1 B1DL99_9BACL AAA ATPase central domain protein Length = 499 Score = 687 bits (1774), Expect = 0.0, Method: Compositional matrix adjust. Identities = 328/490 (66%), Positives = 403/490 (82%) Query: 1 MPKFWKEVLIGFVPVLVIFMAFVGINIFPVIIAAGMVGALLLIAHLRGGLTVNAGGDKKR 60 M K+W E+ GFV ++V+F+AF GINI PV++ A + G + + G G K+ Sbjct: 1 MRKYWVEITAGFVLMVVVFLAFRGINIIPVLLLACLAGGVWFASRGGKGRMTALPGSKRS 60 Query: 61 KKNGPSKLTFEEIGGQDNAKQELREALDFLIRHEEISKFGIRPLKGILLTGPPGTGKTLM 120 K+ ++F++IGGQD AKQEL EALDFL++ +EI+K GIRP+KGILLTGPPGTGKTL+ Sbjct: 61 KQIHAVSISFDQIGGQDRAKQELTEALDFLVKQDEIAKLGIRPMKGILLTGPPGTGKTLL 120 Query: 121 AKAAAHYTNSVFVAASGSEFVEMYVGVGAGRVRDLFKDARARALKENKQSAIIFIDEIDV 180 AKAAAHYT+S+++AASGSEFVEMYVGVGAGR+RDLFK+AR +A K +K+SA+IFIDEIDV Sbjct: 121 AKAAAHYTDSIYLAASGSEFVEMYVGVGAGRIRDLFKEAREKARKASKKSAVIFIDEIDV 180 Query: 181 IGGKREGGQQREYDQTLNQLLTEMDGIYNNDTPRILLVAATNRKEMLDSALLRPGRFDRH 240 IGGKR+GGQ REYDQTLNQLLTEMDGI++++ PRIL++AATNRKEMLDSALLRPGRFDRH Sbjct: 181 IGGKRDGGQHREYDQTLNQLLTEMDGIHSDEAPRILIIAATNRKEMLDSALLRPGRFDRH 240 Query: 241 IQVDMPDKKGRRSILDLHAQNKPLHEEVDLDKIAEEAYGFSGAQLESVMNEAAIYMMREN 300 I VD+PDKKGR IL LHA NKPL E VDL++IA E++GFSGAQLESVMNE AIY MRE Sbjct: 241 IGVDLPDKKGRLHILRLHAANKPLDESVDLERIASESFGFSGAQLESVMNEGAIYAMREQ 300 Query: 301 LTQVEQRHLSLAIDKVMMGEKTDRETNHEEKKRVAIHELGHAIMAELLRPGSVSQVTLTP 360 + ++HL++AIDKVMMGEKTDRE EE++RVA+HELGHAI AEL+RPGSVSQV L+P Sbjct: 301 NETILEQHLAMAIDKVMMGEKTDREATAEERERVALHELGHAISAELVRPGSVSQVALSP 360 Query: 361 RGQALGYVRHNPQTEQYLYTKDYLENQIMIALGGAVAEEMYYGGRSTGSRGDFDQALNIV 420 RGQALGYVRHNPQ ++YLYTK++LE+QIMIALGGA AEE++YGGRSTGSRGDFDQA+NIV Sbjct: 361 RGQALGYVRHNPQQDRYLYTKEFLESQIMIALGGAAAEEIFYGGRSTGSRGDFDQAMNIV 420 Query: 421 ETMMKSGLTSLGIANLQMVTTEELMKENSKILDELMVRTHELLGKQRNVFDYSLDILMKE 480 TM++SGLT LGI + M+T ++ ILDELM +T +L +R VF SLD+L+KE Sbjct: 421 RTMVESGLTELGIIDANMMTPDKWANITRSILDELMRKTKAMLEAKREVFVQSLDVLIKE 480 Query: 481 EVLSGEQFRC 490 E L G+ FR Sbjct: 481 ETLGGDDFRA 490 >C0ZCI2.1 C0ZCI2_BREBN Probable ATP-dependent zinc metallopeptidase EC=3.4.24.- Length = 503 Score = 607 bits (1566), Expect = e-172, Method: Compositional matrix adjust. Identities = 295/490 (60%), Positives = 384/490 (78%), Gaps = 5/490 (1%) Query: 6 KEVLIGFVPVLVIFMAFVGINIFPVIIAAGMVGAL--LLIAHLRG-GLTVNAGGDKKRKK 62 KE+LIG VP L+IF+ F+G+NI P ++ ++GA+ L+I G G V GG +K K Sbjct: 3 KEILIGVVPALLIFLVFLGVNITPFLVFGAVLGAIYFLVIRQQNGQGGNVALGGKRKANK 62 Query: 63 N--GPSKLTFEEIGGQDNAKQELREALDFLIRHEEISKFGIRPLKGILLTGPPGTGKTLM 120 + S + F +IGGQ+ AK+EL+E+LDFL+ ++I ++GIRP+KG+LLTGPPGTGKTLM Sbjct: 63 HELPKSDVQFADIGGQERAKKELKESLDFLVYKDKIEQYGIRPIKGVLLTGPPGTGKTLM 122 Query: 121 AKAAAHYTNSVFVAASGSEFVEMYVGVGAGRVRDLFKDARARALKENKQSAIIFIDEIDV 180 AKAAA+YTNS FVAASGS+FVEMYVGVGA RVR+LF++A+A A K + SAIIFIDEIDV Sbjct: 123 AKAAANYTNSAFVAASGSQFVEMYVGVGAQRVRELFQEAKALAEKNGQDSAIIFIDEIDV 182 Query: 181 IGGKREGGQQREYDQTLNQLLTEMDGIYNNDTPRILLVAATNRKEMLDSALLRPGRFDRH 240 +GGKR+G QQREYDQTLNQLLTEMDG+ D PRIL++AATNRK+MLD+ALLRPGRFDRH Sbjct: 183 VGGKRDGQQQREYDQTLNQLLTEMDGVATTDKPRILVMAATNRKDMLDAALLRPGRFDRH 242 Query: 241 IQVDMPDKKGRRSILDLHAQNKPLHEEVDLDKIAEEAYGFSGAQLESVMNEAAIYMMREN 300 I VD+PDK R IL +H NKPL E+V L+K+A+E +GFSGAQLESV NEAAIY MR+ Sbjct: 243 ISVDLPDKPAREQILTIHTANKPLGEDVTLEKVAQETFGFSGAQLESVANEAAIYAMRDR 302 Query: 301 LTQVEQRHLSLAIDKVMMGEKTDRETNHEEKKRVAIHELGHAIMAELLRPGSVSQVTLTP 360 ++ +H A+DKVM+GEK DR+ + EEKKRVA+HELGHAI++E +RPGSVSQVTL+P Sbjct: 303 QDKITAKHFGYAVDKVMLGEKVDRQASEEEKKRVALHELGHAIVSESVRPGSVSQVTLSP 362 Query: 361 RGQALGYVRHNPQTEQYLYTKDYLENQIMIALGGAVAEEMYYGGRSTGSRGDFDQALNIV 420 RG+ALGYVR NP ++YLYTK+ +E QIM++LGGAVAEE+YYGGRSTGS+ DF+QAL + Sbjct: 363 RGKALGYVRQNPMEDRYLYTKEAMEKQIMVSLGGAVAEEIYYGGRSTGSKNDFEQALGMA 422 Query: 421 ETMMKSGLTSLGIANLQMVTTEELMKENSKILDELMVRTHELLGKQRNVFDYSLDILMKE 480 + +++SG++ LGI +LQ V + E ++L+ L+ T LL + +VF L L++ Sbjct: 423 QEIVQSGMSELGIVHLQYVDKNRIHDEVERLLEGLLAETRNLLRQFDSVFQTGLHTLLEA 482 Query: 481 EVLSGEQFRC 490 EVL G++FR Sbjct: 483 EVLQGDEFRA 492 >A4J5U1.1 A4J5U1_DESRM Vesicle-fusing ATPase EC=3.6.4.6 Length = 494 Score = 511 bits (1316), Expect = e-143, Method: Compositional matrix adjust. Identities = 255/489 (52%), Positives = 345/489 (70%), Gaps = 7/489 (1%) Query: 5 WKEVLIGFVPVLVIFMAFVGINIFPVIIAAGMVGALLLIAHLRGGLTVNAGGDKKRKKNG 64 KE+ +G L++F G +I P I GAL + + + GL G K Sbjct: 2 LKEISLGLGAALLVFGVVAGYDITPFIFLLAFGGALYYLTNAKTGLKSGIGKASTIPKR- 60 Query: 65 PSKLTFEEIGGQDNAKQELREALDFLIRHEEISKFGIRPLKGILLTGPPGTGKTLMAKAA 124 +++FE+IGGQ +A +EL+EALDF+ ++EI + GIRPLKGI++TGPPGTGKTLMA+AA Sbjct: 61 -QEISFEDIGGQSSAIKELKEALDFIKNYQEIKRLGIRPLKGIIMTGPPGTGKTLMARAA 119 Query: 125 AHYTNSVFVAASGSEFVEMYVGVGAGRVRDLFKDARARALKENKQSAIIFIDEIDVIGGK 184 A YT+S+FV+ASGS+FVEMY GVGA RVR +F+ AR A+K+ K+S IIFIDE++V+ K Sbjct: 120 ASYTDSIFVSASGSDFVEMYAGVGAQRVRKIFQTARESAIKQKKKSGIIFIDEVEVLASK 179 Query: 185 R-EGGQQREYDQTLNQLLTEMDGIYNNDTPRILLVAATNRKEMLDSALLRPGRFDRHIQV 243 R + EYDQTLN LL EMDG+ +D R+L++AATNR +M+D ALLRPGRFDR ++V Sbjct: 180 RGQNASHMEYDQTLNALLVEMDGLKVDDNVRLLIMAATNRVDMMDPALLRPGRFDRQVKV 239 Query: 244 DMPDKKGRRSILDLHAQNKPLHEEVDLDKIAEEAYGFSGAQLESVMNEAAIYMMRENLTQ 303 D+PDK GR IL LH NKPL + VDL ++A E +GFSGA LES+ NEAAI MRE L + Sbjct: 240 DLPDKNGRLEILKLHTSNKPLAQNVDLLQVARETFGFSGAHLESLTNEAAILAMREGLKE 299 Query: 304 VEQRHLSLAIDKVMMGEKTDRETNHEEKKRVAIHELGHAIMAELLRPGSVSQVTLTPRGQ 363 + RH A+DKV+MGEK DR N EE KRVA+HE GHA+M+EL RPGSVS +T+TPRG Sbjct: 300 IYHRHFHEAVDKVIMGEKLDRRPNPEELKRVAVHETGHALMSELSRPGSVSALTVTPRGG 359 Query: 364 ALGYVRHNPQTEQYLYTKDYLENQIMIALGGAVAEEMYYGGRSTGSRGDFDQALNIVETM 423 ALGY+R P+ + +LYTKDYLENQI + L GAVAEE+ YG RSTG+ D+++AL ET+ Sbjct: 360 ALGYMRQTPEDDTFLYTKDYLENQIAVMLAGAVAEEVIYGNRSTGASNDYEKALQTAETI 419 Query: 424 MKSGLTSLGIANLQMVTTEELMKEN--SKILDELMVRTHELLGKQRNVFDYSLDILMKEE 481 ++SG++ LG+ +L+ + + +K N S+IL E R + + ++D+L+++E Sbjct: 420 IRSGMSRLGVVSLENLPGD--LKHNVVSEILKEQEQRVMNYITSHKEFLILTMDVLLEKE 477 Query: 482 VLSGEQFRC 490 +SGE FR Sbjct: 478 KISGEDFRS 486 >B7DPS6.1 B7DPS6_9BACL Microtubule-severing ATPase EC=3.6.4.3 Length = 503 Score = 507 bits (1306), Expect = e-142, Method: Compositional matrix adjust. Identities = 266/485 (54%), Positives = 346/485 (71%), Gaps = 6/485 (1%) Query: 6 KEVLIGFVPVLVIFMAFVG-INIFPVIIAAGMVGALLLIAHLRGGLTVNAGGDKKRKKNG 64 +E LIG +V F+ + I I PV+ AG+ GA+L + R AG D + N Sbjct: 4 REWLIGTGIAVVAFLGMLHVIQIIPVLFLAGL-GAMLWVVLDRRNAQSPAGRDVSARPN- 61 Query: 65 PSKLTFEEIGGQDNAKQELREALDFLIRHEEISKFGIRPLKGILLTGPPGTGKTLMAKAA 124 ++F+EIGGQ++AK+EL EALDFL + I++ GIRP+KGILLTGPPGTGKTLMAKAA Sbjct: 62 ---VSFDEIGGQESAKRELMEALDFLRYRDRIAQLGIRPIKGILLTGPPGTGKTLMAKAA 118 Query: 125 AHYTNSVFVAASGSEFVEMYVGVGAGRVRDLFKDARARALKENKQSAIIFIDEIDVIGGK 184 A YT+SVF++ASGSEFVEMYVGVGA RVRDLF+ ARA A +ENK SAIIF+DEIDV+GG+ Sbjct: 119 ATYTDSVFLSASGSEFVEMYVGVGASRVRDLFRRARALAKRENKDSAIIFLDEIDVVGGR 178 Query: 185 REGGQQREYDQTLNQLLTEMDGIYNNDTPRILLVAATNRKEMLDSALLRPGRFDRHIQVD 244 R +EYDQTLNQLLTEMDG+ + P +L++AATNR +MLD ALLRPGRFDR I+VD Sbjct: 179 RGQHSHQEYDQTLNQLLTEMDGMNTSQHPFVLVMAATNRPDMLDPALLRPGRFDRQIRVD 238 Query: 245 MPDKKGRRSILDLHAQNKPLHEEVDLDKIAEEAYGFSGAQLESVMNEAAIYMMRENLTQV 304 +PDK+GR IL + +NKPL +VDL+ IA E +GFSGAQLE+V+NEAAI +R+ + + Sbjct: 239 LPDKEGRLHILRIQTKNKPLAPDVDLEHIARETFGFSGAQLEAVVNEAAIMALRKGESMI 298 Query: 305 EQRHLSLAIDKVMMGEKTDRETNHEEKKRVAIHELGHAIMAELLRPGSVSQVTLTPRGQA 364 Q A+DKV+MGEKT R EE +RVA+HE+GHAI+ ELLRPG+VS +T+ PRG+A Sbjct: 299 RQEMFRDAVDKVLMGEKTGRRPTDEELERVAVHEIGHAIVTELLRPGAVSHITIAPRGRA 358 Query: 365 LGYVRHNPQTEQYLYTKDYLENQIMIALGGAVAEEMYYGGRSTGSRGDFDQALNIVETMM 424 LG+VR P+ +QYLYTK+ LE QI IAL G +AEE+ YG RSTG++ DF QA + TM+ Sbjct: 359 LGFVRQIPEDDQYLYTKEQLEQQIDIALAGCLAEELQYGNRSTGAQNDFQQASQLARTMV 418 Query: 425 KSGLTSLGIANLQMVTTEELMKENSKILDELMVRTHELLGKQRNVFDYSLDILMKEEVLS 484 + GL+ LGI + + + L +E IL E T ELL + +++EE + Sbjct: 419 RCGLSRLGIVDEEHLPERLLEREVRFILAERERATRELLRPYQGEIARLAKQVVREESVD 478 Query: 485 GEQFR 489 G +FR Sbjct: 479 GAEFR 483 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.137 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,902,073,597 Number of extensions: 124404588 Number of successful extensions: 481410 Number of sequences better than 1.0e-05: 7730 Number of HSP's gapped: 454665 Number of HSP's successfully gapped: 8523 Length of query: 500 Length of database: 3,060,696,203 Length adjustment: 140 Effective length of query: 360 Effective length of database: 1,741,754,103 Effective search space: 627031477080 Effective search space used: 627031477080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)