BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0022.p (293 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKW9.1 B3KKW9_9BACI 3-hydroxybutyryl-CoA dehydrogenase EC=1.1.... 441 e-122 B1DL98.1 B1DL98_9BACL 3-hydroxybutyryl-CoA dehydrogenase EC=1.1.... 387 e-106 Q67L77.1 Q67L77_SYMTH 3-hydroxybutyryl-CoA dehydrogenase 260 9e-68 C1DIM5.1 C1DIM5_AZOVD 3-hydroxybutyryl-CoA dehydrogenase 212 4e-53 Q476P6.1 Q476P6_RALEJ 3-hydroxyacyl-CoA dehydrogenase EC=1.1.1.35 206 3e-51 >B3KKW9.1 B3KKW9_9BACI 3-hydroxybutyryl-CoA dehydrogenase EC=1.1.1.157 Length = 290 Score = 441 bits (1133), Expect = e-122, Method: Compositional matrix adjust. Identities = 214/293 (73%), Positives = 240/293 (81%), Gaps = 3/293 (1%) Query: 1 MNFKKXXXXXXXXXXXXXAEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAIT 60 M FKK AEMLAAKGLDV+LVEKT E+LDYSY MIETSLDKQLEKW IT Sbjct: 1 MYFKKIGVVGGGTMGQGIAEMLAAKGLDVLLVEKTPEKLDYSYRMIETSLDKQLEKWGIT 60 Query: 61 QAEKKLILSRIQKVTHFAELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXX 120 QAEKKLIL+RIQKVTHFAELS+CDMVIETI EDL+AK++VF QLDQV H+I Sbjct: 61 QAEKKLILNRIQKVTHFAELSTCDMVIETIIEDLDAKKEVFTQLDQVCP---GHVILASN 117 Query: 121 XXXXXXXXXXXXXMYPERVIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEK 180 YPERVIGMHFIHPV+KVDLVEI+RGLKTSD TF +TK FV+E+ +K Sbjct: 118 TSTLSLTELASSTKYPERVIGMHFIHPVSKVDLVEIIRGLKTSDHTFNETKRFVEEVSDK 177 Query: 181 KGVMIYESPGFVSSRLICLFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFG 240 KG+M+YESPGFV++R+I L INEAMH+LQEGVAS EDIDDAMRIGY FQHGPLEMADRFG Sbjct: 178 KGIMVYESPGFVTTRMITLMINEAMHLLQEGVASAEDIDDAMRIGYNFQHGPLEMADRFG 237 Query: 241 LDSVLAALENMFREYGELKYRPSTILKKMVRAGQLGMKSGEGFFKYDKDGDRI 293 LDS+LAALE MFREYGELKYRPS +LKK+VRAGQLG+K+GEGFFKYDKDGDR+ Sbjct: 238 LDSILAALERMFREYGELKYRPSIVLKKLVRAGQLGVKTGEGFFKYDKDGDRV 290 >B1DL98.1 B1DL98_9BACL 3-hydroxybutyryl-CoA dehydrogenase EC=1.1.1.157 Length = 290 Score = 387 bits (995), Expect = e-106, Method: Compositional matrix adjust. Identities = 182/275 (66%), Positives = 225/275 (81%), Gaps = 3/275 (1%) Query: 19 AEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAITQAEKKLILSRIQKVTHFA 78 AEMLA+KGLDV L+E + ERL Y MIE +LDKQ+EKWA+TQAEKKLIL+RI ++ Sbjct: 19 AEMLASKGLDVYLIESSEERLTYGMQMIELNLDKQIEKWALTQAEKKLILNRIHTCKNYE 78 Query: 79 ELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXXXXXXXXXXXXXXXMYPER 138 +L+SC++VIE+ITEDLE+K++V +LD++ +I +PER Sbjct: 79 DLTSCELVIESITEDLESKKQVIKKLDEICD---DKVIIASNTSTLSLTELASVTGHPER 135 Query: 139 VIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEKKGVMIYESPGFVSSRLIC 198 VIG+HFI+PV+K+DLVEIVRGLKTSD+TF+ TK FVDE+++KKG+M++ESPGFV++RLIC Sbjct: 136 VIGLHFIYPVSKIDLVEIVRGLKTSDATFDATKEFVDEVIQKKGIMVFESPGFVTTRLIC 195 Query: 199 LFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFGLDSVLAALENMFREYGEL 258 LFINEA+HVL+EGVAS EDID+AMRIGY FQ+GP EMADRFGLDSVLAAL+ MFREYGEL Sbjct: 196 LFINEALHVLEEGVASAEDIDNAMRIGYSFQNGPFEMADRFGLDSVLAALDRMFREYGEL 255 Query: 259 KYRPSTILKKMVRAGQLGMKSGEGFFKYDKDGDRI 293 KYRPS +LKKMVR G LG+KSG GFFKYDKDGDRI Sbjct: 256 KYRPSIVLKKMVRGGHLGVKSGIGFFKYDKDGDRI 290 >Q67L77.1 Q67L77_SYMTH 3-hydroxybutyryl-CoA dehydrogenase Length = 296 Score = 260 bits (665), Expect = 9e-68, Method: Compositional matrix adjust. Identities = 124/275 (45%), Positives = 183/275 (66%), Gaps = 4/275 (1%) Query: 19 AEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAITQAEKKLILSRIQKVTHFA 78 A+++A +GL+V++V ++ E L+ + ++ +L ++EKWA+TQ+EK+ IL+RI T Sbjct: 19 AQLVATQGLEVIMVGRSEEELEQARRQLDLALQHEIEKWALTQSEKRAILARISMTTDIN 78 Query: 79 ELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXXXXXXXXXXXXXXXMYPER 138 EL+ D VI T+ ++ +++F LDQV +I P++ Sbjct: 79 ELAKADFVIATLVVEIAEDKEIFRTLDQVCR---REVILASNTSTLSITEMASATNRPDK 135 Query: 139 VIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEKKGVMIYESPGFVSSRLIC 198 VIG HF+ P+ + +V++VRGLKTSD T A ++ + + GV ++ESPG++++RLI Sbjct: 136 VIGCHFLQPIPRTRVVQVVRGLKTSDETVSQVMALMERL-GRTGVEVFESPGYITTRLIV 194 Query: 199 LFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFGLDSVLAALENMFREYGEL 258 INEA VL EGVAS EDID AMR+G++ GPLE+ADR GLD+VL E ++REYG+L Sbjct: 195 PLINEACQVLMEGVASAEDIDTAMRLGFEMPRGPLEIADRIGLDTVLVMAERLWREYGDL 254 Query: 259 KYRPSTILKKMVRAGQLGMKSGEGFFKYDKDGDRI 293 KYRP+ ILKK+VRAG LG+++GEGFFKYD DG RI Sbjct: 255 KYRPAPILKKLVRAGHLGVETGEGFFKYDADGARI 289 >C1DIM5.1 C1DIM5_AZOVD 3-hydroxybutyryl-CoA dehydrogenase Length = 291 Score = 212 bits (539), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 108/268 (40%), Positives = 164/268 (61%), Gaps = 4/268 (1%) Query: 19 AEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAITQAEKKLILSRIQKVTHFA 78 A++ A G V+LV+ + L+ A + +L++Q++K I +A+++ L RIQ TH+ Sbjct: 27 AQVCALAGRQVILVDISDNALERGIATLSGNLERQVKKGLIDEADRQAALGRIQPQTHYG 86 Query: 79 ELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXXXXXXXXXXXXXXXMYPER 138 L+ ++VIE +E+LE K+++ +++ S + PER Sbjct: 87 ALAGSELVIEAASENLELKRQILTLIEEATE---SDALIASNTSSLSISELASVLGKPER 143 Query: 139 VIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEKKGVMIYESPGFVSSRLIC 198 IG+HF +PV + LVE++RGL+T+++T+ AF +I K + + SPGFV +R++C Sbjct: 144 FIGLHFFNPVPVMTLVEVIRGLQTAEATYAKALAFAGQI-GKTAISVRNSPGFVVNRILC 202 Query: 199 LFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFGLDSVLAALENMFREYGEL 258 INEA+ VLQEG+AS EDID MR+G GPL +AD GLD++LA LE R++G+ Sbjct: 203 PMINEAIFVLQEGLASAEDIDTGMRLGCNHPIGPLALADLIGLDTLLAILETFHRDFGDP 262 Query: 259 KYRPSTILKKMVRAGQLGMKSGEGFFKY 286 KYRPST+LK+MV AG LG KSG GF Y Sbjct: 263 KYRPSTLLKEMVAAGYLGRKSGRGFHSY 290 >Q476P6.1 Q476P6_RALEJ 3-hydroxyacyl-CoA dehydrogenase EC=1.1.1.35 Length = 284 Score = 206 bits (523), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 105/270 (38%), Positives = 162/270 (60%), Gaps = 4/270 (1%) Query: 19 AEMLAAKGLDVMLVEKTAERLDYSYAMIETSLDKQLEKWAITQAEKKLILSRIQKVTHFA 78 A+ A GLDV++V+ + + A + SLD+ ++K I++A+K L+RI T + Sbjct: 19 AQACAVVGLDVVMVDISDAAVQKGIATVAGSLDRLIKKDKISEADKMTALARIHGSTAYD 78 Query: 79 ELSSCDMVIETITEDLEAKQKVFNQLDQVXXXXGSHIIXXXXXXXXXXXXXXXXXMYPER 138 +L D+VIE TE+ + K K+ Q+D + G ++I PER Sbjct: 79 DLKKADIVIEAATENFDLKVKILKQIDSIV---GENVIIASNTSSISITKLAAVTSRPER 135 Query: 139 VIGMHFIHPVAKVDLVEIVRGLKTSDSTFEDTKAFVDEIVEKKGVMIYESPGFVSSRLIC 198 IGMHF +PV + LVE++RGL+TSD+ D +A E+ K + + SPGFV +R++C Sbjct: 136 FIGMHFFNPVPVMALVELIRGLQTSDAAHADVEALAKEL-GKYPITVKNSPGFVVNRILC 194 Query: 199 LFINEAMHVLQEGVASPEDIDDAMRIGYQFQHGPLEMADRFGLDSVLAALENMFREYGEL 258 INEA VL EG+ASPE+ID+ M++G GPL +AD GLD++LA +E ++ E+ + Sbjct: 195 PMINEAFCVLGEGLASPEEIDEGMKLGCNHPIGPLALADMIGLDTMLAVMEVLYTEFADP 254 Query: 259 KYRPSTILKKMVRAGQLGMKSGEGFFKYDK 288 KYRP+ ++++MV AG LG K+G G + Y K Sbjct: 255 KYRPAMLMREMVAAGYLGRKTGRGVYVYSK 284 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.320 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,384,231,583 Number of extensions: 49311760 Number of successful extensions: 141516 Number of sequences better than 1.0e-05: 3040 Number of HSP's gapped: 135416 Number of HSP's successfully gapped: 3293 Length of query: 293 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 159 Effective length of database: 1,798,280,193 Effective search space: 285926550687 Effective search space used: 285926550687 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)