BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0021.p (402 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKX0.1 B3KKX0_9BACI Acetate kinase 702 0.0 B1DL97.1 B1DL97_9BACL Acetate kinase 604 e-171 B0TGV2.1 ACKA_HELMI Acetate kinase EC=2.7.2.1 494 e-138 Q67L76.1 ACKA_SYMTH Acetate kinase EC=2.7.2.1 491 e-137 B4BH76.1 B4BH76_CLOTM Acetate kinase 480 e-134 >B3KKX0.1 B3KKX0_9BACI Acetate kinase Length = 402 Score = 702 bits (1811), Expect = 0.0, Method: Compositional matrix adjust. Identities = 334/401 (83%), Positives = 370/401 (92%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 MNILVIN+GSSSLKYQLY+MTDESVLAKGLVERIGMDSSIL HKPTGKQ+VTEVSEILEH Sbjct: 1 MNILVINAGSSSLKYQLYDMTDESVLAKGLVERIGMDSSILTHKPTGKQDVTEVSEILEH 60 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 TAIRKVL LT++EHGVI SI+ I AVGHRVVHGGE FK SALVD+DAK +IR+LFDLA Sbjct: 61 TTAIRKVLGMLTNEEHGVIDSIESIQAVGHRVVHGGESFKESALVDSDAKAEIRRLFDLA 120 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNPA++MGI A E NMPGVPQVVVFDTAFHQTMPEK+Y+YAIPRVLYNKYKVRRYGAH Sbjct: 121 PLHNPASMMGIKAAETNMPGVPQVVVFDTAFHQTMPEKSYLYAIPRVLYNKYKVRRYGAH 180 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH +VS+AAA++L RPLEDLKIITCHIGNGAS+TAV GG+SIDTSMGMTPLEGLMMGT Sbjct: 181 GTSHAYVSQAAAEFLNRPLEDLKIITCHIGNGASLTAVKGGKSIDTSMGMTPLEGLMMGT 240 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 RSGDLDPAIVP+VMNKEEL+V EVNSMLNKHSGLLAISG+SSDMR+I +G EKG+PNSTL Sbjct: 241 RSGDLDPAIVPFVMNKEELTVNEVNSMLNKHSGLLAISGISSDMREITEGMEKGDPNSTL 300 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 AF+MYEYR+RKYIGSYAAAMNGVDVIVFTAGVGEN+ VLR +VL LT+LG+ELD E N Sbjct: 301 AFEMYEYRVRKYIGSYAAAMNGVDVIVFTAGVGENSIVLRQRVLEQLTYLGVELDEELNS 360 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQGIN 401 +RSG+PRRIS SKV+VLV+PTNEELVIARDT+RIVQ N Sbjct: 361 IRSGEPRRISAANSKVEVLVIPTNEELVIARDTHRIVQASN 401 >B1DL97.1 B1DL97_9BACL Acetate kinase Length = 402 Score = 604 bits (1558), Expect = e-171, Method: Compositional matrix adjust. Identities = 285/399 (71%), Positives = 344/399 (86%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 M +LVIN+GSSSLKYQLYNM +E+VLA G VERIGMDSSI+ H+P GK +VT+VSEIL+H Sbjct: 1 MKVLVINAGSSSLKYQLYNMENETVLASGRVERIGMDSSIVTHEPPGKPDVTQVSEILDH 60 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 TA++KV+ LT EHGV++S+ EI+AVGHR+VHGGE FK+SALV A+AK +I++LFDLA Sbjct: 61 ITAVKKVIDMLTHPEHGVVASMGEIDAVGHRIVHGGEVFKSSALVTAEAKQEIKKLFDLA 120 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNPA +MGI+A E NMP VPQVVVFDTAFHQTMP +Y+Y IPRVLY ++KVRRYG H Sbjct: 121 PLHNPAHMMGINAVETNMPNVPQVVVFDTAFHQTMPNTSYLYPIPRVLYRRHKVRRYGFH 180 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH +VS+ AA YL +PLE LK+I+CHIGNGAS TA+ G+S DTSMGMTPLEGLMMGT Sbjct: 181 GTSHQYVSQKAAKYLGKPLESLKMISCHIGNGASATAILDGKSFDTSMGMTPLEGLMMGT 240 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 RSGDLDPAIVPYVMNKEEL++ EVNSMLNKHSG+LAISG+SSDMR+I++ ++G+ N+ L Sbjct: 241 RSGDLDPAIVPYVMNKEELTLSEVNSMLNKHSGMLAISGISSDMREIVEAMDEGDANAKL 300 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 AFDMY YR+RKYIG+YAAAMNGVDVI+FTAGVGEN+ VLR V ++FLG+ELD E N Sbjct: 301 AFDMYTYRVRKYIGAYAAAMNGVDVILFTAGVGENSNVLRKAVCEGISFLGVELDEERNN 360 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQG 399 +RS + R IST S+V+VLVVPTNEEL+IARDTY IV+G Sbjct: 361 IRSKEVREISTDASRVKVLVVPTNEELLIARDTYAIVKG 399 >B0TGV2.1 ACKA_HELMI Acetate kinase EC=2.7.2.1 Length = 397 Score = 494 bits (1272), Expect = e-138, Method: Compositional matrix adjust. Identities = 235/399 (58%), Positives = 307/399 (76%), Gaps = 2/399 (0%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 M +LVINSGSSSLKYQL++M E++LAKGLVERIG+ S+L H+P GK +V +EIL H Sbjct: 1 MIVLVINSGSSSLKYQLFDMRKEALLAKGLVERIGLSGSMLTHRPIGKDQVVIETEILNH 60 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 + AI+ + LT ++HGV++S+ EI+AVGHRV HGG+ F SA+V+ + IR LF++A Sbjct: 61 DRAIQLTVEALTHEDHGVVASMREIDAVGHRVAHGGDTFSDSAVVNEQSLANIRALFEVA 120 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNP AVMGI A + +PGVPQV VFDTAFHQT+P AY YA+P L K+ +RRYG H Sbjct: 121 PLHNPPAVMGIEACQHMLPGVPQVAVFDTAFHQTIPGYAYNYALPYELAAKHSLRRYGFH 180 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH +V++ AA L RPLEDL+IITCH+GNGAS+TA+D G+S+DTSMG TPLEGL+MGT Sbjct: 181 GTSHKYVAQWAAAMLGRPLEDLRIITCHLGNGASITAIDRGRSVDTSMGFTPLEGLIMGT 240 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 R GD+DPA VP++M KE L+ G++N ++NK SG+L +SGVSSD RD+ + A KG L Sbjct: 241 RVGDMDPAAVPFLMEKEGLTTGQINDLMNKKSGVLGVSGVSSDFRDLEEEAAKGNERCQL 300 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 A DM+ YR++KYIG+YAA MNGVD IVFTAG+GEN+ +R V + L++LG++LD E NK Sbjct: 301 ALDMFAYRVKKYIGAYAAVMNGVDAIVFTAGLGENSPSMRQSVCSGLSYLGVQLDEEKNK 360 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQG 399 R IS + +V+VVPTNEEL+IARDTYR+++G Sbjct: 361 GRG--EADISAAGATCRVMVVPTNEELMIARDTYRLLKG 397 >Q67L76.1 ACKA_SYMTH Acetate kinase EC=2.7.2.1 Length = 398 Score = 491 bits (1263), Expect = e-137, Method: Compositional matrix adjust. Identities = 234/398 (58%), Positives = 298/398 (74%), Gaps = 2/398 (0%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 M ILV+N GSSS+KYQL +M ESVLA G VER+GM+ +I +K G +++ E+ IL+H Sbjct: 1 MKILVLNCGSSSIKYQLLDMETESVLAIGKVERVGMEDAIFEYKGAG-EKIKEIGTILDH 59 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 +RKVL LTD HGVI + +EI AVGHRVVHGGE F S ++ + K +R+LFDLA Sbjct: 60 TAGVRKVLKALTDPVHGVIQAPEEIAAVGHRVVHGGEAFVKSVIITDEVKRTLRELFDLA 119 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNPA + GI A E +P P V VFDTAFH TMP KA++YA+P +Y +KVRRYG H Sbjct: 120 PLHNPANLTGILAAEAALPHAPGVAVFDTAFHATMPRKAFLYALPYAMYKGHKVRRYGFH 179 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH +VS+ AAD L RPLE++ +ITCH+GNG+SVTAV GG+S+DTSMG TPL GL+MGT Sbjct: 180 GTSHKYVSQRAADLLGRPLEEVNLITCHLGNGSSVTAVQGGKSVDTSMGFTPLAGLIMGT 239 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 R+GDLDP ++PY+M +EE+ + EVNSMLNKHSG+L ISG+SSDMR I + EKG+P + Sbjct: 240 RAGDLDPGVIPYIMAREEIGISEVNSMLNKHSGILGISGISSDMRQIEEEMEKGDPRAVE 299 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 F+M EYRLRKYIG+YAA + VD IVFT G+GEN+ + R V NL FLG+ D EAN Sbjct: 300 TFEMMEYRLRKYIGAYAAVLGRVDGIVFTGGIGENSPLFRKAVCQNLGFLGVTFDEEANN 359 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQ 398 R G R IS +SKV V+V+PTNEEL+IAR+T +++ Sbjct: 360 CR-GQERIISGPDSKVAVMVIPTNEELMIARETAELIR 396 >B4BH76.1 B4BH76_CLOTM Acetate kinase Length = 399 Score = 480 bits (1236), Expect = e-134, Method: Compositional matrix adjust. Identities = 225/400 (56%), Positives = 306/400 (76%), Gaps = 1/400 (0%) Query: 1 MNILVINSGSSSLKYQLYNMTDESVLAKGLVERIGMDSSILNHKPTGKQEVTEVSEILEH 60 MNILVIN+GSSSLKYQL +MT+ESVLAKG+ +RIG++ S L H TG + V ++ H Sbjct: 1 MNILVINTGSSSLKYQLIDMTNESVLAKGVCDRIGLEHSFLKHTKTGGETVVIEKDLYNH 60 Query: 61 NTAIRKVLACLTDKEHGVISSIDEINAVGHRVVHGGEFFKASALVDADAKTKIRQLFDLA 120 AI++V++ LTD++ GVI S+ EI+AVGHR+VHGGE FK SA++D D IR +LA Sbjct: 61 KLAIQEVISALTDEKIGVIKSMSEISAVGHRIVHGGEKFKESAIIDEDVMKAIRDCVELA 120 Query: 121 PLHNPAAVMGISATEVNMPGVPQVVVFDTAFHQTMPEKAYMYAIPRVLYNKYKVRRYGAH 180 PLHNP+ ++GI A + +P VP V VFDTAFHQTMP AY+YA+P +Y KYK+R+YG H Sbjct: 121 PLHNPSNIIGIEACKQILPDVPMVAVFDTAFHQTMPRHAYIYALPYEIYEKYKLRKYGFH 180 Query: 181 GTSHDFVSKAAADYLERPLEDLKIITCHIGNGASVTAVDGGQSIDTSMGMTPLEGLMMGT 240 GTSH +V+ AA L +P+E LK+ITCH+GNGAS+ AV GG+S+DTSMG TPL+GL MGT Sbjct: 181 GTSHKYVAHRAAQMLGKPIESLKLITCHLGNGASICAVKGGKSVDTSMGFTPLQGLCMGT 240 Query: 241 RSGDLDPAIVPYVMNKEELSVGEVNSMLNKHSGLLAISGVSSDMRDIIDGAEKGEPNSTL 300 RSG++DPA++ Y+M KE++++ ++N+ LNK SG+L ISGVSSD RD+ D AEKG+ + L Sbjct: 241 RSGNVDPAVITYLMEKEKMNINDINNFLNKKSGVLGISGVSSDFRDVQDAAEKGDDRAQL 300 Query: 301 AFDMYEYRLRKYIGSYAAAMNGVDVIVFTAGVGENAAVLRAKVLNNLTFLGIELDAEANK 360 A D++ Y +RKYIG Y A +NGVD +VFTAG+GEN A +R +VL ++ F GI++D + N+ Sbjct: 301 ALDIFCYGVRKYIGKYIAVLNGVDAVVFTAGIGENNAYIRREVLKDMDFFGIKIDLDKNE 360 Query: 361 VRSGDPRRISTVESKVQVLVVPTNEELVIARDTYRIVQGI 400 V+ G IS ++KV+ LV+PTNEEL IAR+T R+V+ + Sbjct: 361 VK-GKEADISAPDAKVKTLVIPTNEELEIARETLRLVKNL 399 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.316 0.133 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,241,484,102 Number of extensions: 91794179 Number of successful extensions: 247861 Number of sequences better than 1.0e-05: 1981 Number of HSP's gapped: 242864 Number of HSP's successfully gapped: 1983 Length of query: 402 Length of database: 3,060,696,203 Length adjustment: 138 Effective length of query: 264 Effective length of database: 1,760,596,133 Effective search space: 464797379112 Effective search space used: 464797379112 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)