BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0020.p (431 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKX1.1 B3KKX1_9BACI Asparaginyl-tRNA synthetase 797 0.0 C0ZCI4.1 C0ZCI4_BREBN Asparaginyl-tRNA synthetase EC=6.1.1.22 712 0.0 A8VXZ0.1 A8VXZ0_9BACI Amidohydrolase 684 0.0 B7GHT8.1 B7GHT8_ANOFW Asparaginyl-tRNA synthetase 668 0.0 Q65I63.1 SYN_BACLD Asparaginyl-tRNA synthetase EC=6.1.1.22 657 0.0 >B3KKX1.1 B3KKX1_9BACI Asparaginyl-tRNA synthetase Length = 431 Score = 797 bits (2059), Expect = 0.0, Method: Compositional matrix adjust. Identities = 371/431 (86%), Positives = 405/431 (93%) Query: 1 MANKSVIKNVNEHVGEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQVWD 60 MA KSVI+NVN+HVGE V IG W+NNKRSSGKIQFLQLRDG+GYIQGVVVKSEV EQVW+ Sbjct: 1 MATKSVIRNVNQHVGETVTIGAWINNKRSSGKIQFLQLRDGSGYIQGVVVKSEVSEQVWE 60 Query: 61 DAKSLTQESSLYVTGIIREEPRSQSGYEMTVTGIEVLHLTENYPITPKEHGVDFLMDHRH 120 DAKSLTQESSLYVTG+IREEPRSQSGYEMTVTGIE++HLTENYPITPKEHGVD+LMDHRH Sbjct: 61 DAKSLTQESSLYVTGVIREEPRSQSGYEMTVTGIEIIHLTENYPITPKEHGVDYLMDHRH 120 Query: 121 LWLRSAKQRAVLVIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLFHTKYFEEDA 180 LWLR+ KQRA++VIRAEIIRAVQ++F+ NGFT VDPPILTPTSAEGTTNLFHTKYF+EDA Sbjct: 121 LWLRTQKQRAIMVIRAEIIRAVQEYFDTNGFTLVDPPILTPTSAEGTTNLFHTKYFDEDA 180 Query: 181 YLTQSGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESLRVQ 240 YLTQSGQLYMEAAAMALG+VYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESL+VQ Sbjct: 181 YLTQSGQLYMEAAAMALGKVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESLQVQ 240 Query: 241 EEFISFVVQSVLKNCRAELEAVGRDISKLENIKAPFPRITYDDAIKFLNEKGYEIAWGED 300 E+FIS VVQSV+KNCR ELE +GRDISKLE I+APFPRITYD+AI+FL+ + ++I WGED Sbjct: 241 EQFISHVVQSVVKNCRQELETIGRDISKLEAIQAPFPRITYDEAIEFLHTQDFDIPWGED 300 Query: 301 FGAPHETAIEKANDKPVFITHYPASFKAFYMKPHPERPEVVLCADMIAPEGYGEIIGGSQ 360 FGAPHETAI + DKPVFITHYPA KAFYMKP P PEVVLCADMIAPEGYGEIIGGSQ Sbjct: 301 FGAPHETAIAEKYDKPVFITHYPAGIKAFYMKPDPNCPEVVLCADMIAPEGYGEIIGGSQ 360 Query: 361 RIDDPALLEERFKEHNLSMDAYKWYMDLRTYGSVPHSGFGLGLERTVAWICGLDHVRETI 420 RIDDP LLEERFKEH+LSM+ Y+WYMDLR YG+VPHSGFGLGLERTVAWICGLDHVRETI Sbjct: 361 RIDDPQLLEERFKEHDLSMETYQWYMDLRKYGTVPHSGFGLGLERTVAWICGLDHVRETI 420 Query: 421 PFPRTLYRLYP 431 PFPRTLYR+YP Sbjct: 421 PFPRTLYRMYP 431 >C0ZCI4.1 C0ZCI4_BREBN Asparaginyl-tRNA synthetase EC=6.1.1.22 Length = 429 Score = 712 bits (1839), Expect = 0.0, Method: Compositional matrix adjust. Identities = 326/427 (76%), Positives = 374/427 (87%) Query: 5 SVIKNVNEHVGEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQVWDDAKS 64 + I + +HVG+ V +GCW+ NKRSSGKIQFLQLRDG+G+IQGVVVK+EV E+VW++A Sbjct: 3 TTIARIGQHVGQEVRLGCWLYNKRSSGKIQFLQLRDGSGFIQGVVVKAEVAEEVWENASQ 62 Query: 65 LTQESSLYVTGIIREEPRSQSGYEMTVTGIEVLHLTENYPITPKEHGVDFLMDHRHLWLR 124 LTQESSLY+TG +R + R+ SGYE+TVTG+E++ + + YPI+ KEHGVDFLMDHRHLWLR Sbjct: 63 LTQESSLYITGSVRADDRAPSGYELTVTGVEIIQIAQEYPISLKEHGVDFLMDHRHLWLR 122 Query: 125 SAKQRAVLVIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLFHTKYFEEDAYLTQ 184 S +QRAV+ +R+E+IRA+ +FF+ENGF KVDPPILTPTSAEGTTNLFHTKYF+EDAYL+Q Sbjct: 123 SPRQRAVMAVRSEVIRAMYEFFHENGFVKVDPPILTPTSAEGTTNLFHTKYFDEDAYLSQ 182 Query: 185 SGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESLRVQEEFI 244 SGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAF DHEE+LR+QE F+ Sbjct: 183 SGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFVDHEENLRIQEAFV 242 Query: 245 SFVVQSVLKNCRAELEAVGRDISKLENIKAPFPRITYDDAIKFLNEKGYEIAWGEDFGAP 304 S VVQSVLKNC EL+ + RD +KL+N+ FPRI+YDDAIK L EKG EI WG+DFGAP Sbjct: 243 SHVVQSVLKNCERELKTLERDTTKLQNVTGSFPRISYDDAIKLLQEKGSEIKWGDDFGAP 302 Query: 305 HETAIEKANDKPVFITHYPASFKAFYMKPHPERPEVVLCADMIAPEGYGEIIGGSQRIDD 364 ET I DKPVFITHYP KAFYMKPHPERPEVVLCADMIAPEGYGEIIGGSQRIDD Sbjct: 303 DETMIADHFDKPVFITHYPTEIKAFYMKPHPERPEVVLCADMIAPEGYGEIIGGSQRIDD 362 Query: 365 PALLEERFKEHNLSMDAYKWYMDLRTYGSVPHSGFGLGLERTVAWICGLDHVRETIPFPR 424 P LLE+RF EH LS +AY+WYMDLR YG+VPHSGFGLGLERT+AWICGLDHVRETIPFPR Sbjct: 363 PELLEKRFAEHELSEEAYRWYMDLRKYGTVPHSGFGLGLERTIAWICGLDHVRETIPFPR 422 Query: 425 TLYRLYP 431 L RLYP Sbjct: 423 MLNRLYP 429 >A8VXZ0.1 A8VXZ0_9BACI Amidohydrolase Length = 429 Score = 684 bits (1764), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/428 (73%), Positives = 370/428 (86%) Query: 4 KSVIKNVNEHVGEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQVWDDAK 63 K+ I V ++V ++V IG W+ NKRSSGKI FLQLRDGTG+IQGVVVK+EV E ++ AK Sbjct: 2 KTTISEVGKYVDQSVTIGAWLANKRSSGKIAFLQLRDGTGFIQGVVVKAEVEEDIFKLAK 61 Query: 64 SLTQESSLYVTGIIREEPRSQSGYEMTVTGIEVLHLTENYPITPKEHGVDFLMDHRHLWL 123 LTQESSLYVTG +RE+ R+ SGYE+TV +EV+ + E+YPITPKEHG +FLMDHRHLWL Sbjct: 62 ELTQESSLYVTGKVREDDRAPSGYELTVESVEVIQMAEDYPITPKEHGTEFLMDHRHLWL 121 Query: 124 RSAKQRAVLVIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLFHTKYFEEDAYLT 183 RS KQ A++ IR EIIRA +FFNE GFTK+DPPILT +SAEGTTNLFHTKYF+EDAYL+ Sbjct: 122 RSRKQHAIMKIRNEIIRATYEFFNEQGFTKIDPPILTGSSAEGTTNLFHTKYFDEDAYLS 181 Query: 184 QSGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESLRVQEEF 243 QSGQLYMEAAAMA G+V+SFGPTFRAEKSKTRRHLIEFWMIEPEMAF DH+ESL +QE + Sbjct: 182 QSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFMDHDESLVLQENY 241 Query: 244 ISFVVQSVLKNCRAELEAVGRDISKLENIKAPFPRITYDDAIKFLNEKGYEIAWGEDFGA 303 +SFVVQ+VL+NC+ EL+ + RD + LE I APFPRI+YD+AI FL ++GYEI WGEDFGA Sbjct: 242 VSFVVQAVLQNCKLELKTLERDTATLEKISAPFPRISYDEAISFLQKEGYEIEWGEDFGA 301 Query: 304 PHETAIEKANDKPVFITHYPASFKAFYMKPHPERPEVVLCADMIAPEGYGEIIGGSQRID 363 PHETAI +A++KPVFIT+YP KAFYMKPHPER +VVLCAD+IAPEGYGEIIGGSQRID Sbjct: 302 PHETAIAEAHEKPVFITNYPKDIKAFYMKPHPERDDVVLCADLIAPEGYGEIIGGSQRID 361 Query: 364 DPALLEERFKEHNLSMDAYKWYMDLRTYGSVPHSGFGLGLERTVAWICGLDHVRETIPFP 423 D L+++R+ EHNLS DAYKWY+DLR YG+VPHSGFGLGLERTVAWI G +HVRETIPFP Sbjct: 362 DAELMKQRYAEHNLSEDAYKWYLDLRKYGTVPHSGFGLGLERTVAWISGTEHVRETIPFP 421 Query: 424 RTLYRLYP 431 R L RLYP Sbjct: 422 RLLNRLYP 429 >B7GHT8.1 B7GHT8_ANOFW Asparaginyl-tRNA synthetase Length = 454 Score = 668 bits (1724), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/429 (71%), Positives = 372/429 (86%), Gaps = 1/429 (0%) Query: 4 KSVIKNVNEHVGEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQVWDDAK 63 K+ I V+++VG+ V IG W+ NKRSSGKI FLQLRDGTG+IQGVV K++V E V+ AK Sbjct: 26 KTTIAEVHKYVGQEVTIGAWLANKRSSGKIAFLQLRDGTGFIQGVVEKAQVDEDVFQTAK 85 Query: 64 SLTQESSLYVTGIIREEPRSQSGYEMTVTGIEVLHLTENYPITPKEHGVDFLMDHRHLWL 123 S+TQESSLYVTG++RE+ RS GYE++VT IE++H +YPITPKEHGV+FLMDHRHLWL Sbjct: 86 SMTQESSLYVTGVVREDARSPFGYELSVTNIEIIHTAVDYPITPKEHGVEFLMDHRHLWL 145 Query: 124 RSAKQRAVLVIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLFHTKYFEEDAYLT 183 RS +Q A++ IR E+IRA +FFNENGF KVDPPILT ++ EGTT LFHTKYF+EDAYL+ Sbjct: 146 RSKRQHAIMKIRNEVIRATYEFFNENGFVKVDPPILTGSAPEGTTELFHTKYFDEDAYLS 205 Query: 184 QSGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESLRVQEEF 243 QSGQLYMEAAAMALG+V+SFGPTFRAEKSKTRRHLIEFWMIEPEMAF E++L+VQE++ Sbjct: 206 QSGQLYMEAAAMALGKVFSFGPTFRAEKSKTRRHLIEFWMIEPEMAFYQFEDNLQVQEQY 265 Query: 244 ISFVVQSVLKNCRAELEAVGRDISKLENIKAPFPRITYDDAIKFLNEKGY-EIAWGEDFG 302 +S +VQSVLK+C+ EL+ +GRD SKLEN+K PF RITYD+AI+ L+EKG+ +I WG+DFG Sbjct: 266 VSHIVQSVLKHCQLELKTLGRDTSKLENVKPPFARITYDEAIQLLHEKGFDDIQWGDDFG 325 Query: 303 APHETAIEKANDKPVFITHYPASFKAFYMKPHPERPEVVLCADMIAPEGYGEIIGGSQRI 362 APHETAI ++ DKPVFITHYP + K FYM+P P RPEVVLCAD+IAPEGYGEIIGGS+RI Sbjct: 326 APHETAIAESFDKPVFITHYPTTLKPFYMQPDPNRPEVVLCADLIAPEGYGEIIGGSERI 385 Query: 363 DDPALLEERFKEHNLSMDAYKWYMDLRTYGSVPHSGFGLGLERTVAWICGLDHVRETIPF 422 D LL++R +EH LS+DAYKWY++LR YGSVPHSGFGLGLERT+AWICG++HVRETIPF Sbjct: 386 HDYDLLKQRLEEHQLSLDAYKWYLELRQYGSVPHSGFGLGLERTIAWICGVEHVRETIPF 445 Query: 423 PRTLYRLYP 431 PR L RLYP Sbjct: 446 PRLLNRLYP 454 >Q65I63.1 SYN_BACLD Asparaginyl-tRNA synthetase EC=6.1.1.22 Length = 430 Score = 657 bits (1694), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/429 (71%), Positives = 359/429 (83%), Gaps = 1/429 (0%) Query: 4 KSVIKNVNEHVGEAVVIGCWVNNKRSSGKIQFLQLRDGTGYIQGVVVKSEVPEQVWDDAK 63 K+ I V +HVGE V IG W+ NKRSSGKI FLQLRDGTG+IQGVVVK+EV E+ + AK Sbjct: 2 KTTINQVYKHVGEEVTIGAWIANKRSSGKIAFLQLRDGTGFIQGVVVKAEVDEETFQTAK 61 Query: 64 SLTQESSLYVTGIIREEPRSQSGYEMTVTGIEVLHLTENYPITPKEHGVDFLMDHRHLWL 123 S+TQE+SLYV G+++E+ RS GYE+ VT +EV+H +YPITPKEHG +FLMDHRHLWL Sbjct: 62 SVTQETSLYVKGVVKEDERSPLGYELAVTSLEVIHEATDYPITPKEHGTEFLMDHRHLWL 121 Query: 124 RSAKQRAVLVIRAEIIRAVQQFFNENGFTKVDPPILTPTSAEGTTNLFHTKYFEEDAYLT 183 RS +Q A + IR EIIRA +FFN+ GF KVDPPILT ++ EGTT LF TKYF+EDAYL+ Sbjct: 122 RSKRQHATMKIRNEIIRATYEFFNKEGFVKVDPPILTGSAPEGTTELFATKYFDEDAYLS 181 Query: 184 QSGQLYMEAAAMALGRVYSFGPTFRAEKSKTRRHLIEFWMIEPEMAFTDHEESLRVQEEF 243 QSGQLYMEAAAMALG+V+SFGPTFRAEKSKT+RHLIEFWMIEPEMAF + E+L VQE + Sbjct: 182 QSGQLYMEAAAMALGKVFSFGPTFRAEKSKTKRHLIEFWMIEPEMAFVEFNENLEVQENY 241 Query: 244 ISFVVQSVLKNCRAELEAVGRDISKLENIKAPFPRITYDDAIKFLNEKGY-EIAWGEDFG 302 +SF+VQSVL+NC+ EL +GRD SKLE IKAPFPRITYD+AI+FL EKG+ +I WG+DFG Sbjct: 242 VSFIVQSVLENCKIELNTLGRDTSKLELIKAPFPRITYDEAIQFLKEKGFDDIEWGDDFG 301 Query: 303 APHETAIEKANDKPVFITHYPASFKAFYMKPHPERPEVVLCADMIAPEGYGEIIGGSQRI 362 APHETAI ++ DKPVFITHYP S K FYM+P P+R +VVLCAD+IAPEGYGEIIGGS+RI Sbjct: 302 APHETAIAESYDKPVFITHYPTSLKPFYMQPAPDREDVVLCADLIAPEGYGEIIGGSERI 361 Query: 363 DDPALLEERFKEHNLSMDAYKWYMDLRTYGSVPHSGFGLGLERTVAWICGLDHVRETIPF 422 D LLE R KEH L DAY+WY +LR YGSVPHSGFGLGLERTVAWICG HVRETIPF Sbjct: 362 HDLELLEARLKEHGLESDAYQWYAELRKYGSVPHSGFGLGLERTVAWICGSPHVRETIPF 421 Query: 423 PRTLYRLYP 431 PR L RLYP Sbjct: 422 PRLLNRLYP 430 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.136 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,655,758,125 Number of extensions: 112297871 Number of successful extensions: 261207 Number of sequences better than 1.0e-05: 4573 Number of HSP's gapped: 251990 Number of HSP's successfully gapped: 4669 Length of query: 431 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 292 Effective length of database: 1,751,175,118 Effective search space: 511343134456 Effective search space used: 511343134456 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)