BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0018.p (288 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKY9.1 B3KKY9_9BACI Pyrroline-5-carboxylate reductase EC=1.5.1.2 392 e-107 B1DL87.1 B1DL87_9BACL Pyrroline-5-carboxylate reductase EC=1.5.1.2 266 2e-69 C0ZL56.1 C0ZL56_BREBN Pyrroline-5-carboxylate reductase EC=1.5.1.2 235 3e-60 C2ZU12.1 C2ZU12_BACCE Pyrroline-5-carboxylate reductase 229 3e-58 C2ZCI3.1 C2ZCI3_BACCE Pyrroline-5-carboxylate reductase 229 3e-58 >B3KKY9.1 B3KKY9_9BACI Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 289 Score = 392 bits (1007), Expect = e-107, Method: Compositional matrix adjust. Identities = 193/289 (66%), Positives = 233/289 (80%), Gaps = 1/289 (0%) Query: 1 MCQQPAI-PLLNHQIVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXY 59 MCQ P PL++ I FYGAGSMAEAIVRG+++R+VV NI MLN Y Sbjct: 1 MCQSPQNRPLIDKAITFYGAGSMAEAIVRGLVSRSVVLPENITMLNRSNAQRLLELSERY 60 Query: 60 GVLGSNDPEQKNEYLRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQ 119 GV +ND K E L+T+PVIVLAMKPKDAA+AL+ LGP LS DQL+IS+IAG++I T Q Sbjct: 61 GVQINNDEAAKIEILKTSPVIVLAMKPKDAAKALKELGPKLSSDQLIISVIAGLSIYTTQ 120 Query: 120 GLLGKSQPVVRTMPNTSSSIGLGATGIAYSKEVDEQSRRTALNIFEAVGLTSVIDEERME 179 LLG QP+ RTMPNTS+SIGLG+TGIA+SKE+ E+ R+ L +FEAVG +VI+EE+M+ Sbjct: 121 TLLGNQQPIARTMPNTSASIGLGSTGIAFSKEISEEQRQLVLTLFEAVGHVTVIEEEKMD 180 Query: 180 TLTGISGSGPAYIYYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALR 239 LTGISGSGPAY YYMMEAM AAGIRGGL EQSRELT+QT+LGAARMVQQTGE+P++LR Sbjct: 181 ILTGISGSGPAYFYYMMEAMTAAGIRGGLTPEQSRELTLQTILGAARMVQQTGEQPSSLR 240 Query: 240 KKVTSPNGSTQAALEVLEKGDFFETVISAVNRCAERSREMGLALEKELS 288 K+TSPNGSTQAALE L+KGDFFETVI+AVNRCAERS+EMG L++++S Sbjct: 241 AKITSPNGSTQAALETLDKGDFFETVIAAVNRCAERSKEMGAMLKEQIS 289 >B1DL87.1 B1DL87_9BACL Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 293 Score = 266 bits (680), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 132/273 (48%), Positives = 191/273 (69%) Query: 13 QIVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXYGVLGSNDPEQKNE 72 +I FYGAGSMAEAIVRG+I + + + I MLN YGV + + Sbjct: 17 KISFYGAGSMAEAIVRGLINQKLTKPEQISMLNRQNADRLNELSEKYGVQTVLNGSTNEQ 76 Query: 73 YLRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQGLLGKSQPVVRTM 132 +++ A ++ LAMKPKDA A+ S+ LLS QL++S+IAG++I +++ +LG+S +VRTM Sbjct: 77 FIQDADIVFLAMKPKDAVSAISSIKHLLSEKQLIVSVIAGLSISSIERVLGRSAAIVRTM 136 Query: 133 PNTSSSIGLGATGIAYSKEVDEQSRRTALNIFEAVGLTSVIDEERMETLTGISGSGPAYI 192 PNTSS+IGLG TGI+YS V E+ R+ A +F ++G+ +V++E +++ G+SGSGPAY+ Sbjct: 137 PNTSSTIGLGVTGISYSGSVTEEQRKLAETMFASIGINAVVEESLQDSIVGVSGSGPAYV 196 Query: 193 YYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALRKKVTSPNGSTQAA 252 Y+ MEAM+ A + GLPA+Q++EL VQTVLGAA MV+ TGEEPA LR+KVTSPNG+TQAA Sbjct: 197 YFFMEAMMEAAEKLGLPADQAKELIVQTVLGAAEMVRTTGEEPAELRRKVTSPNGTTQAA 256 Query: 253 LEVLEKGDFFETVISAVNRCAERSREMGLALEK 285 +E + + V+ + R AER+ E+G +E+ Sbjct: 257 IESMAASGLTDAVVRGMLRSAERAGEIGADIER 289 >C0ZL56.1 C0ZL56_BREBN Pyrroline-5-carboxylate reductase EC=1.5.1.2 Length = 280 Score = 235 bits (600), Expect = 3e-60, Method: Compositional matrix adjust. Identities = 127/275 (46%), Positives = 181/275 (65%), Gaps = 3/275 (1%) Query: 6 AIPLLNHQIVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXYGVLGSN 65 A + +I F GAGS+ EA++ G+ + ++ S I + N YGV + Sbjct: 5 ATAITEGRIGFLGAGSIVEAMLSGIGKKGLLPSERISVTNRNNMERLEQLANDYGVTVTQ 64 Query: 66 DPEQKNEYLRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQGLLGKS 125 D K + +R++ +++LA+KPKDAAEAL L ++ DQL+IS+IAG++ + LG Sbjct: 65 D---KFDVVRSSDILILAIKPKDAAEALLDLRGVVRSDQLIISVIAGVSTSLIGEWLGAE 121 Query: 126 QPVVRTMPNTSSSIGLGATGIAYSKEVDEQSRRTALNIFEAVGLTSVIDEERMETLTGIS 185 P++RTMPNTSS++GL ATG+ + V E+ A +FEA+G + EE ++ +TG+S Sbjct: 122 CPIIRTMPNTSSAVGLSATGLCANPFVQEEHCELATKLFEAIGTVYEVAEEELDIITGLS 181 Query: 186 GSGPAYIYYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALRKKVTSP 245 GSGPAYIYY++EAM+ AG + GL E +R+LT+QTV+GAA M+ T EEP+ LR+KVTSP Sbjct: 182 GSGPAYIYYLVEAMMGAGAKAGLDREMARQLTLQTVIGAAHMLLDTREEPSILRQKVTSP 241 Query: 246 NGSTQAALEVLEKGDFFETVISAVNRCAERSREMG 280 G+TQA LEVLE F E V +A+ R AERSREMG Sbjct: 242 GGTTQAGLEVLEAYQFQEAVTAAILRAAERSREMG 276 >C2ZU12.1 C2ZU12_BACCE Pyrroline-5-carboxylate reductase Length = 279 Score = 229 bits (583), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 116/273 (42%), Positives = 180/273 (65%), Gaps = 3/273 (1%) Query: 13 QIVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXYGVLGSNDPEQKNE 72 I F GAGS+AEAI+ G++ NVV+ +I + N YGV G+++ K E Sbjct: 5 NISFLGAGSIAEAIIGGLLNANVVKGEHITVSNRSNETRLQELHTKYGVKGTHN---KKE 61 Query: 73 YLRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQGLLGKSQPVVRTM 132 L A ++ LAMKPKD AEA+ L + D L+IS++AG++ +++ LL K P++R M Sbjct: 62 LLADANILFLAMKPKDVAEAMIPLKEYIHNDLLIISLLAGVSTHSIRNLLQKDVPIIRAM 121 Query: 133 PNTSSSIGLGATGIAYSKEVDEQSRRTALNIFEAVGLTSVIDEERMETLTGISGSGPAYI 192 PNTS++I AT I+ S+ E+ R A ++FE +GL SV++EE M +T +SGSGPAYI Sbjct: 122 PNTSAAILKSATAISPSEHTTEEHIRIATSLFETIGLVSVVEEEDMHAVTALSGSGPAYI 181 Query: 193 YYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALRKKVTSPNGSTQAA 252 YY++EAM A + GL + ++ L +QT++GAA M++ + + P+ LRK++TSP G+T+A Sbjct: 182 YYVVEAMEEAAKKIGLKEDVAKSLILQTMIGAAEMLKASEKHPSILRKEITSPGGTTEAG 241 Query: 253 LEVLEKGDFFETVISAVNRCAERSREMGLALEK 285 +EVL++ DF +T+IS + + +RS ++G LEK Sbjct: 242 IEVLQEHDFQQTLISCITQATKRSHDLGKTLEK 274 >C2ZCI3.1 C2ZCI3_BACCE Pyrroline-5-carboxylate reductase Length = 279 Score = 229 bits (583), Expect = 3e-58, Method: Compositional matrix adjust. Identities = 116/273 (42%), Positives = 180/273 (65%), Gaps = 3/273 (1%) Query: 13 QIVFYGAGSMAEAIVRGMIARNVVESGNIVMLNXXXXXXXXXXXXXYGVLGSNDPEQKNE 72 I F GAGS+AEAI+ G++ NVV+ +I + N YGV G+++ K E Sbjct: 5 NISFLGAGSIAEAIIGGLLNANVVKGEHITVSNRSNETRLQELHTKYGVKGTHN---KKE 61 Query: 73 YLRTAPVIVLAMKPKDAAEALRSLGPLLSPDQLVISMIAGMTIRTMQGLLGKSQPVVRTM 132 L A ++ LAMKPKD AEA+ L + D L+IS++AG++ +++ LL K P++R M Sbjct: 62 LLADANILFLAMKPKDVAEAMIPLKEYIHNDLLIISLLAGVSTHSIRNLLQKDVPIIRAM 121 Query: 133 PNTSSSIGLGATGIAYSKEVDEQSRRTALNIFEAVGLTSVIDEERMETLTGISGSGPAYI 192 PNTS++I AT I+ S+ E+ R A ++FE +GL SV++EE M +T +SGSGPAYI Sbjct: 122 PNTSAAILKSATAISPSEHTTEEHIRIATSLFETIGLVSVVEEEDMHAVTALSGSGPAYI 181 Query: 193 YYMMEAMIAAGIRGGLPAEQSRELTVQTVLGAARMVQQTGEEPAALRKKVTSPNGSTQAA 252 YY++EAM A + GL + ++ L +QT++GAA M++ + + P+ LRK++TSP G+T+A Sbjct: 182 YYVVEAMEEAAKKIGLKEDVAKSLILQTMIGAAEMLKASEKHPSILRKEITSPGGTTEAG 241 Query: 253 LEVLEKGDFFETVISAVNRCAERSREMGLALEK 285 +EVL++ DF +T+IS + + +RS ++G LEK Sbjct: 242 IEVLQEHDFQQTLISCITQATKRSHDLGKTLEK 274 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.315 0.131 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 1,442,433,037 Number of extensions: 52084481 Number of successful extensions: 158626 Number of sequences better than 1.0e-05: 2068 Number of HSP's gapped: 155642 Number of HSP's successfully gapped: 2070 Length of query: 288 Length of database: 3,060,696,203 Length adjustment: 134 Effective length of query: 154 Effective length of database: 1,798,280,193 Effective search space: 276935149722 Effective search space used: 276935149722 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)