BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0543.0016.p (458 letters) Database: pfamseq 9,421,015 sequences; 3,060,696,203 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value B3KKZ1.1 B3KKZ1_9BACI Glutamate 5-kinase 447 e-124 B1DL84.1 B1DL84_9BACL Glutamate 5-kinase EC=2.7.2.11 407 e-111 C3BKH6.1 C3BKH6_9BACI Glutamate 5-kinase 288 9e-76 C2QDC3.1 C2QDC3_BACCE Glutamate 5-kinase 287 1e-75 C3B3N8.1 C3B3N8_BACMY Glutamate 5-kinase 287 2e-75 >B3KKZ1.1 B3KKZ1_9BACI Glutamate 5-kinase Length = 389 Score = 447 bits (1151), Expect = e-124, Method: Compositional matrix adjust. Identities = 217/313 (69%), Positives = 253/313 (80%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 VVKIGSSSLT EGGLN +VAFFA E+AGL++ G EVLLVTSGAVAAGFR IGY RPK Sbjct: 28 VVKIGSSSLTTDEGGLNLDSVAFFAGELAGLREAGHEVLLVTSGAVAAGFREIGYETRPK 87 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 LLHEKQA+AA+GQ +LM+AYQ A A + + AQILLTR DF +R+ MNNA MT+ ELL+Q Sbjct: 88 LLHEKQASAALGQALLMRAYQNALASHQLKAAQILLTRMDFANRKRMNNASMTITELLKQ 147 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 G +PI NENDTVS+DELKFGDNDTLSALVANL++A L++LTD DGLY+ DPRK P A + Sbjct: 148 GVIPIINENDTVSIDELKFGDNDTLSALVANLVRAQQLIILTDTDGLYTADPRKSPDAIK 207 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 YE + +IT EIY++ MRSKIDAAKIATRGGVPVF+G+A EPGDL A Sbjct: 208 YEEIHDITDEIYSMAGGAGSSVGTGGMRSKIDAAKIATRGGVPVFIGKANEPGDLVKALE 267 Query: 241 GTGKGTYFDTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEGS 300 +GKGTYF T LS+LPVKKQWLGFMS+PLG + VD GA +AL+HGGHSLLPVGVKRIEGS Sbjct: 268 HSGKGTYFATHLSALPVKKQWLGFMSSPLGRIVVDPGAEKALIHGGHSLLPVGVKRIEGS 327 Query: 301 FHSGDVVEVLGPD 313 FH+GDVVEV+ D Sbjct: 328 FHAGDVVEVVNMD 340 >B1DL84.1 B1DL84_9BACL Glutamate 5-kinase EC=2.7.2.11 Length = 368 Score = 407 bits (1045), Expect = e-111, Method: Compositional matrix adjust. Identities = 199/311 (63%), Positives = 239/311 (76%), Gaps = 2/311 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 VVKIGSSSLT EGGLNR+ + +FA+E+A L K GC ++LVTSGAVAAGFR IGY ARPK Sbjct: 6 VVKIGSSSLTSEEGGLNRERITYFASELAALHKQGCPLILVTSGAVAAGFRQIGYAARPK 65 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 +HEKQAAAAVGQ +LMQAYQEAF E+GI AQILLTR DF +R+ + NA+ T+EELLR Sbjct: 66 AVHEKQAAAAVGQALLMQAYQEAFGEFGIGVAQILLTRADFSNRKRIQNALKTIEELLRL 125 Query: 121 GAVPIFNENDTVSVDEL--KFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGA 178 +PI NENDTV+ DEL KFGDND LSALVA + KAS L+++TDMDGLY+ DPRK+P A Sbjct: 126 RIIPIINENDTVATDELELKFGDNDKLSALVAAMTKASSLVMITDMDGLYTEDPRKNPEA 185 Query: 179 TRYERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLA 238 + ERV++I+ EI + MRSKI+AA+I+ RGGV F+G+ +PGDLQLA Sbjct: 186 VKIERVDQISEEIMQLAGGAGSAVGTGGMRSKIEAARISMRGGVRAFIGKVQQPGDLQLA 245 Query: 239 AAGTGKGTYFDTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIE 298 GTGKGTYFDT L SLP+KKQWLGF S P G + VD+GA ALL G SLLP G++ I+ Sbjct: 246 LNGTGKGTYFDTQLHSLPMKKQWLGFHSNPHGRIIVDDGAEHALLVRGKSLLPAGIREIQ 305 Query: 299 GSFHSGDVVEV 309 G FHSGDVVEV Sbjct: 306 GDFHSGDVVEV 316 >C3BKH6.1 C3BKH6_9BACI Glutamate 5-kinase Length = 367 Score = 288 bits (737), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 151/317 (47%), Positives = 206/317 (64%), Gaps = 1/317 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 V+KIGSSSL +GG++ + ++ A +A L+++G E+LL+TSGAVAAGF A+GYP RP Sbjct: 7 VIKIGSSSLAANQGGISSEQLSDHVAALARLKQEGHEILLITSGAVAAGFSALGYPGRPV 66 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 + KQAAAAVGQ +LMQAY E F +YGI TAQ+LLTR+DF + +NA T+ ELL + Sbjct: 67 TIKGKQAAAAVGQSLLMQAYTEEFLKYGIVTAQLLLTRSDFSRKEQYSNAYATLSELLDR 126 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 A+PI NEND++SV+EL FGDND LSALV+ L+ A L++ TD++GLY +P+K+P A + Sbjct: 127 SALPIINENDSISVEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDRNPQKNPDAKK 186 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 Y + ++T EI A+ MRSKIDAAK A GV VF+G + Sbjct: 187 YYFLPDVTEEIDALAGDAGSKLGTGGMRSKIDAAKTALSLGVSVFIGTGSGQEKFLDVLK 246 Query: 241 GTGKGTYF-DTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 G G GTY +T + + KQW+ S G + VD GA A++H G SLLP GV + G Sbjct: 247 GKGDGTYIGNTPQKVIKMNKQWIALHSLVRGQIEVDAGAAAAIVHHGKSLLPAGVTGVFG 306 Query: 300 SFHSGDVVEVLGPDARL 316 F +G+VVEV+ R+ Sbjct: 307 IFQAGEVVEVITQKGRV 323 >C2QDC3.1 C2QDC3_BACCE Glutamate 5-kinase Length = 376 Score = 287 bits (735), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 151/317 (47%), Positives = 203/317 (64%), Gaps = 1/317 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 VVKIGSSSL GG+++K ++ A +A L+++G EVLL+TSGAVAAGF A+GYP+RP Sbjct: 16 VVKIGSSSLADSHGGISKKQISDHVAALARLKEEGHEVLLITSGAVAAGFSALGYPSRPV 75 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 + KQAAAAVGQ +LMQAY E F +YGI TAQ+LLTR+DF + +NA T+ ELL + Sbjct: 76 TIKGKQAAAAVGQSLLMQAYTEEFRKYGIVTAQLLLTRSDFSRKEQYSNAYATLGELLNR 135 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 A+PI NEND++S++EL FGDND LSALV+ L+ A L++ TD++GLY +P+KH A + Sbjct: 136 SALPIINENDSISLEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDKNPQKHADAKK 195 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 Y + E+T EI ++ M+SKIDAAK A GV VF+G Sbjct: 196 YYFLPEVTEEIASLAGEAGSKLGTGGMKSKIDAAKTALSLGVSVFIGTGRGQEKFVDVLK 255 Query: 241 GTGKGTYF-DTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 G G GTY + + + KQW+ S G + VD GAV A++ G SLLP GV + G Sbjct: 256 GKGDGTYVGNAPQKEMKMNKQWIALHSLVSGQIEVDAGAVTAIIQQGKSLLPAGVTNVSG 315 Query: 300 SFHSGDVVEVLGPDARL 316 F G+VVEV+ R+ Sbjct: 316 FFQVGEVVEVVTQQGRV 332 >C3B3N8.1 C3B3N8_BACMY Glutamate 5-kinase Length = 367 Score = 287 bits (734), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 151/317 (47%), Positives = 205/317 (64%), Gaps = 1/317 (0%) Query: 1 VVKIGSSSLTGPEGGLNRKAVAFFAAEIAGLQKDGCEVLLVTSGAVAAGFRAIGYPARPK 60 V+KIGSSSL +GG++ + ++ A +A L+++G E+LL+TSGAVAAGF A+GYP RP Sbjct: 7 VIKIGSSSLAANQGGISSEQLSDHVAALARLKQEGHEILLITSGAVAAGFSALGYPGRPV 66 Query: 61 LLHEKQAAAAVGQVMLMQAYQEAFAEYGIPTAQILLTRTDFCSRRAMNNAVMTVEELLRQ 120 + KQAAAAVGQ +LMQAY E F +YGI TAQ+LLTR+DF + +NA T+ ELL + Sbjct: 67 TIKGKQAAAAVGQSLLMQAYTEEFLKYGIVTAQLLLTRSDFSRKEQYSNAYATLSELLDR 126 Query: 121 GAVPIFNENDTVSVDELKFGDNDTLSALVANLLKASHLLVLTDMDGLYSGDPRKHPGATR 180 A+PI NEND++SV+EL FGDND LSALV+ L+ A L++ TD++GLY +P+K+P A + Sbjct: 127 SALPIINENDSISVEELTFGDNDMLSALVSGLVSADMLMIFTDVNGLYDRNPQKNPDAKK 186 Query: 181 YERVEEITPEIYAIXXXXXXXXXXXXMRSKIDAAKIATRGGVPVFVGRATEPGDLQLAAA 240 Y + ++T EI A+ MRSKIDAAK A GV VF+G Sbjct: 187 YYFLPDVTEEIDALAGDAGSKLGTGGMRSKIDAAKTALSLGVSVFIGTGRGQEKFLDVLK 246 Query: 241 GTGKGTYF-DTTLSSLPVKKQWLGFMSTPLGSLYVDEGAVEALLHGGHSLLPVGVKRIEG 299 G G GTY +T + + KQW+ S G + VD GA A++H G SLLP GV + G Sbjct: 247 GKGDGTYIGNTPQKVIKMNKQWIALHSLVRGQIEVDAGAAAAIVHHGKSLLPAGVTGVFG 306 Query: 300 SFHSGDVVEVLGPDARL 316 F +G+VVEV+ R+ Sbjct: 307 IFQAGEVVEVITQKGRV 323 Database: pfamseq Posted date: Aug 5, 2010 3:22 PM Number of letters in database: 3,060,696,203 Number of sequences in database: 9,421,015 Lambda K H 0.319 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 9421015 Number of Hits to DB: 2,527,168,105 Number of extensions: 98516838 Number of successful extensions: 236009 Number of sequences better than 1.0e-05: 1497 Number of HSP's gapped: 234257 Number of HSP's successfully gapped: 1497 Length of query: 458 Length of database: 3,060,696,203 Length adjustment: 139 Effective length of query: 319 Effective length of database: 1,751,175,118 Effective search space: 558624862642 Effective search space used: 558624862642 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)